The invention provides an electrified cleaning agent for precise instruments and a preparation method of the electrified cleaning agent. The cleaning agent comprises cocamidopropyl betaine, nonylphenol polyoxyethylene ether, tetradecyl dimethyl benzyl ammonium, sodium tripolyphosphate, trisodium phosphate, sodium bicarbonate, sodium nitrite, 2-phenoxyethyl alcohol, pine oil, an antistatic agent, polyhydroxy polyamine, ethyl acetate and ethyl alcohol. The preparation method comprises the following steps: firstly adding cocamidopropyl betaine, nonylphenol polyoxyethylene ether, tetradecyl dimethyl benzyl ammonium and sodium tripolyphosphate to ethyl alcohol, so as to obtain a mixture I; adding trisodium phosphate, sodium bicarbonate, the sodium nitrite and 2-phenoxyethyl alcohol to ethyl acetate, so as to obtain a mixture II; and finally mixing the mixture I with the mixture II, and then adding the residual components, so as to obtain the cleaning agent. The cleaning agent is excellent in performance, and can be used as the electrified cleaning agent for the precise instruments.