Biological oil displacement agent and production method and use thereof

An oil washing agent and biological technology, applied in the field of oil extraction industry, can solve the problems of high investment risk, large impact, secondary pollution of the formation, etc.

Inactive Publication Date: 2009-06-10
BEIJING FLTY GASOLINEEUM SCI& TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the block removal and displacement methods adopted in the oil field include: (1) Acidification method, which basically uses strong acids such as hydrochloric acid and hydrofluoric acid. Dissolve the cement in the rock skeleton, resulting in loose formation and sand production. If the gas and insoluble matter generated during the acidification process are not removed in time, the acidification residual acid will cause secondary pollution of the formation
In addition, this acid treatment also has the problem of corroding metal pipelines, wellbore and other equipment, and the H 2 S will also cause some damage to the human body, and acidification and plugging removal are not suitable for the transformation of acid-sensitive formations
(2) Fracturing method, this method is an effective measure for the transformation of tight reservoirs, which can improve the conductivity and increase production capacity, but the investment risk of fracturing operation is high and the process is compli

Method used

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  • Biological oil displacement agent and production method and use thereof
  • Biological oil displacement agent and production method and use thereof
  • Biological oil displacement agent and production method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0157] Embodiment 1: the preparation of biological oil washing agent of the present invention

[0158] (1) Strain rejuvenation

[0159] Take the preserved Methylomonas (Methylomonas) LT1 strain, scrape a small amount of slant culture medium consisting of beef extract 0.5g, peptone 1.0g, NaCl 0.5g, agar 1.5-2g, deionized water 100mL, pH 7.2 with an inoculation needle Then, cultured at 37°C for 12 hours, activated twice in succession, and then measured the ability of the strain to produce an oil-washing agent. The yield of crude biosurfactant is >11.0g / L; the yield of crude bioenzyme is >2.0g / L.

[0160] (2) Shake flask seed culture

[0161] Pick a loop of the above-mentioned activated strain and inoculate it in a 150mL solution containing 20g of glucose, 2g of yeast extract, 5g of NaCl, NaNO 3 2g, MgSO 4 0.25g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 5g, K 2 HPO 4 10g, peptone 1g, deionized water 1000ml, pH adjusted to 7.2 in a 500mL shake flask of the seed medium, placed ...

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Abstract

The invention relates to a biological oil cleaning agent, a method for producing the same and application thereof. The biological oil cleaning agent contains 1.85 to 2.75g/L of coarse biological enzyme extract and 9.75 to 13.45g/L of coarse biosurfactant product. The biological oil cleaning agent has good effect of deplugging and oil displacement; a core displacement test shows the permeability restoration ratio of rock core deplugged by the biological oil cleaning agent averagely exceeds 95 percent and can further improve the extraction yield by over 17 percent on the basis of driving water to ultimate water content.

Description

【Technical field】 [0001] The invention relates to the technical field of oil exploitation industry. More specifically, the present invention relates to bio-washing agents and their production methods and their use. 【Background technique】 [0002] During the production operations such as drilling, well completion, well workover, well killing, well washing, sand washing, acidizing, fracturing and oil production in oil fields, various oil layer damages are often caused, such as emulsification blockage, water lock, suspended particle blockage, Paraffin and colloidal asphaltene deposits, scale, etc., especially the composite plugging of inorganic scale, solid particles and organic scale (paraffin, colloidal asphaltene) wrapped together, and changes in the wettability of oil layer rocks, all of which have caused serious damage to the oil field. Normal production has brought adverse effects. [0003] At present, the block removal and displacement methods adopted in the oil field ...

Claims

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Application Information

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IPC IPC(8): C09K8/58C09K8/52C12N1/20E21B43/22E21B37/06C12R1/26
Inventor 王建国
Owner BEIJING FLTY GASOLINEEUM SCI& TECH
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