Biological oil displacement agent and production method and use thereof
An oil washing agent and biological technology, applied in the field of oil extraction industry, can solve the problems of high investment risk, large impact, secondary pollution of the formation, etc.
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Embodiment 1
[0157] Embodiment 1: the preparation of biological oil washing agent of the present invention
[0158] (1) Strain rejuvenation
[0159] Take the preserved Methylomonas (Methylomonas) LT1 strain, scrape a small amount of slant culture medium consisting of beef extract 0.5g, peptone 1.0g, NaCl 0.5g, agar 1.5-2g, deionized water 100mL, pH 7.2 with an inoculation needle Then, cultured at 37°C for 12 hours, activated twice in succession, and then measured the ability of the strain to produce an oil-washing agent. The yield of crude biosurfactant is >11.0g / L; the yield of crude bioenzyme is >2.0g / L.
[0160] (2) Shake flask seed culture
[0161] Pick a loop of the above-mentioned activated strain and inoculate it in a 150mL solution containing 20g of glucose, 2g of yeast extract, 5g of NaCl, NaNO 3 2g, MgSO 4 0.25g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 5g, K 2 HPO 4 10g, peptone 1g, deionized water 1000ml, pH adjusted to 7.2 in a 500mL shake flask of the seed medium, placed ...
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