Biological oil displacement agent and production method and use thereof
A production method and technology of oil washing agent, applied in the field of biological oil washing agent, can solve problems such as unsuitable for acid-sensitive formations, secondary pollution of formations, adverse effects of derivatives, etc.
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Embodiment 1
[0157] Embodiment 1: the preparation of biological oil washing agent of the present invention
[0158] (1) Strain rejuvenation
[0159] Take the preserved Methylomonas (Methylomonas) LT1 strain, scrape a small amount of slant culture medium consisting of beef extract 0.5g, peptone 1.0g, NaCl 0.5g, agar 1.5-2g, deionized water 100mL, pH 7.2 with an inoculation needle Then, cultured at 37°C for 12 hours, activated twice in succession, and then measured the ability of the strain to produce an oil-washing agent. The yield of crude biosurfactant is >11.0g / L; the yield of crude bioenzyme is >2.0g / L.
[0160] (2) Shake flask seed culture
[0161] Pick a loop of the above-mentioned activated strain and inoculate it in a 150mL solution containing 20g of glucose, 2g of yeast extract, 5g of NaCl, NaNO 3 2g, MgSO 4 0.25g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 5g, K 2 HPO 4 10g, peptone 1g, deionized water 1000ml, pH adjusted to 7.2 in a 500mL shake flask of the seed medium, placed on a sh...
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