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41 results about "Kefir Grain" patented technology

Method for preparing Kefir sour milk

The invention discloses a method for preparing Kefir sour milk which comprises water, sugar and sour milk prepared from the fermentation of two different Kefir grains; the preparing method comprises the follow steps: 1. raw milk is sterilized and then cooled to the temperature of 20 DEG C to 30 DEG C; 2. the two different Kefir grains are activated and cultured separately; 3. the activated and cultured Kefir grains with inoculum size of 2 percent to 5 percen are respectively inoculated to the sterilized raw milk; 4. the raw milk is fermented for 15 hours to 24 hours at the temperature of 20 DEG C to 30 DEG C; 5. the Kefir grains are filtered out to obtain two filtrates which are fermentation agents; 6. the two fermentation agents are mixed; 7. the mixed fermentation agents with the inoculum size of 2 percent to 5 percent are inoculated to the sterilized original milks and then fermented at the variable temperature ranging from 20 DEG C to 45 DEG C for 4 hours to 6 hours; and 8. the fermented milk are blended, homogenized and canned. With the adoption of the fermentation agents for fermenting at the variable temperature, the flavor of Kefir milk is well improved. The ferment time of the product is short, the yeasts in the mixed fermentation agents are more stable and the difference between flavors of Kefir sour milk prepared in different batches is small. The product has health-care functions of regulating beneficial bacteria in intestinal canals, lowering blood lipids, resisting tumors and the like, thus being a low-cost functional beverage.
Owner:于岚 +2

Kefiranofaciens subsp.kefiranofaciens ZW3 strain

The present invention relates to a Kefiranofaciens subsp.kefiranofaciens ZW3 strain which is obtained from the Kefir grains through filtering separation and is preserved in Laboratory of culture collection of the Microbiology, Chinese Academy of Science. The preservation data is December 18, 2008, and the preserving number is CGMCC2809. The ZW3 strain of the invention has the advantages of excellent thermal stability, strong emulsifying capacity and high yield of exopolysaccharide. The exopolysaccharide which is obtained by separating and purifying the fermentation broth of the ZW3 strain is the macromolecule biological polysaccharides. The exopolysaccharide not only can be used as the thickening agent and the emulsifier in the food industry, but also can be used as the probiotics for promoting the growth of other probiotics in the intestional canal. The exopolysaccharide is also related with the functions of anti-tumor, anti-fistula, immunoregulation, cholesterol lowering or blood pressure regulating of the probiotics. Furthermore the exopolysaccharide has biodegradability and has no harm to the human body and the environment. The exopolysaccharide can be used as an effective flocculant in the food fields of wastewater treatment, drinking water product, downstream processing, etc.
Owner:TIANJIN UNIV OF SCI & TECH

Bean yoghourt fermenting agent with processing suitability as well as preparation method and application thereof

The invention discloses a bean yoghourt fermenting agent with processing suitability as well as a preparation method and application thereof, and belongs to the field of bioengineering. The preparation method of the bean yoghourt fermenting agent with the processing suitability comprises the following steps: an exogenous microbial population is added into reconstituted milk, and the exogenous microbial population, the reconstituted milk and Kefir grains are mixed and fermented; after multi-generation domestication, a Kefir fermenting agent is reformed, so that the microbial population structure of the reformed Kefir fermenting agent is changed, and the reformed Kefir fermenting agent is used for fermenting bean milk so as to produce bean yoghourt; soybean isoflavone can be biologically converted, so that concentration of genistein and daidzein in the bean yoghourt is improved; the reformed Kefir grains are adopted to prepare the fermenting agent so as to ferment the beam milk, so that the Kefir bean yoghourt is smooth in taste and rich in nutrition, absorption and utilization of vegetable protein for a human body can be improved, and probiotics in the bean yoghourt is increased; the soybean isoflavone can be biologically converted, so that the concentration of the genistein and the daidzein in the bean yoghourt is improved, and the bioavailability of the soybean isoflavone is improved.
Owner:SOUTH CHINA AGRI UNIV

Prebiotic-probiotic compound micro-ecological preparation and preparation method thereof

The invention discloses a prebiotic-probiotic compound micro-ecological preparation and a preparation method thereof. The preparation method of the prebiotic-probiotic compound micro-ecological preparation comprises the following main steps of selecting lactic acid bacteria, yeast and bacillus subtilis as bacterial strains of the prebiotic-probiotic compound micro-ecological preparation, wherein the lactic acid bacteria and the yeast are from Kefir grains; separately activating the Kefir grains and the bacillus subtilis, and mixing the activated Kefir grains and bacillus subtilis at a ratio of3 to 1; inoculating a culture medium with the mixed probiotic stains, wherein the volume of the mixed probiotic stains accounts for 3-10% of the volume of the fermenataion liquid, so as to be subjected to fermentation culture; adding prebiotics, adding skimmed milk powder, trehalose, monosodium glutamate and glycerine as protective agents, carrying out uniform mixing, and performing low-temperature spray-drying on the mixture so as to obtain the micro-ecological preparation. The prebiotic-probiotic compound micro-ecological preparation is characterized by containing the lactic acid bacteria,the yeast and the bacillus subtilis, so that, micro-ecological balance of the body can be jointly adjusted; moreover, the prebiotic-probiotic compound micro-ecological preparation also contains preparation substances, so that, body health can be better improved so as to enhance body immunity.
Owner:吉林师范大学博达学院

Novel polypeptide preservative and preparation method thereof

The present invention provides a novel polypeptide preservative and a preparation method thereof. The preparation method comprises the following steps: Tibet Kefir grains at the late logarithmic growth stage are inoculated into pure milk, the pure milk is subjected to sealed static fermentation at 30-40 DEG C until a part of curd is produced; the pure milk is added, a liquid fermented milk is drawn in a sterile environment and coated on a MC-solid medium and a MRS solid medium plate respectively, and the mixture is then sealed and cultured; after completion of the culture, bacterial colonies and the medium in the culture dish are added into pure milk, and at the same time, carbon and nitrogen sources are added, and the mixture is placed in a temperature of 30-35 DEG C to be fermented for 65-75 hours; and after the completion of the fermentation, the fermented milk is centrifuged to remove precipitates, the pH value of the resulted supernatant is adjusted to 2-5, the treated fermented milk is maintained at 8-10 DEG C for 60-120 min, then the fermented milk is centrifuged, the obtained precipitate is dried into a powder, and the powder is subjected to cryopreservation, thereby obtaining the natural novel polypeptide preservative. The present invention also provides a use method of the natural novel polypeptide preservative. The novel polypeptide preservative is safe and hygienic, natural and non-toxic, high-efficient, and broad in spectrum and stable in performance, and can be widely applied in the field of fresh fruit preservative.
Owner:SOUTH CHINA AGRI UNIV

Kefir naan

The invention provides Kefir naan. The Kefir naan comprises the following raw materials in percentage by weight: 60-70% of flour, 1-3% of peanut oil, 0.5-1% of Kefir grains, 0.5-1% of salt, 20-25% ofwater (or 10-15% of milk and 10-15% of water), 5-10% of egg liquid and 2-5% of auxiliary materials. A preparation method of the Kefir naan comprises the following steps: adding the flour, salt, activated kefir grains, water and egg liquid into a dough mixer, fermenting for 2-4 hours after dough making, fully kneading and pressing to prepare a naan blank with a size of 15-30 cm and a thickness of about 0.5 cm, pricking patterns on a surface by using a naan needle, brushing a layer of oil, smearing or scattering (or adding into dough) auxiliary materials (nuts, grains or spices), baking at a temperature of about 220 DEG C, fully cooling (or continuously cutting the naan into small blocks with a length of 5-10 cm and a width of 3-5 cm) after baking is finished, and performing vacuum packagingby using a PE packaging bag to obtain a finished product. A starter provided by the invention contains various microorganisms such as lactic acid bacteria and saccharomycetes, has better fermentationspeed, taste and flavor of naan than traditional starters, and the Kefir naan has a longer shelf life than traditional fermentation naan.
Owner:TIANJIN UNIV OF SCI & TECH

Household simple Tibetan kefir grain yogurt fermentation device with extrusion and filtration screen bag

The invention discloses a household simple Tibetan kefir grain yogurt fermentation device with an extrusion and filtration screen bag. The household simple Tibetan kefir grain yogurt fermentation device comprises two fermentation cups with covers and one extrusion and filtration screen bag, wherein the extrusion and filtration screen bag is prepared from a nylon mesh, the aperture of the extrusion and filtration screen bag is 20-60 meshes, the extrusion and filtration screen bag is positioned inside fermentation cup bodies, and the extrusion and filtration screen bag are identical to inner cavities of the fermentation cups in shape and size; when in use, the extrusion and filtration screen bag is placed in one cup, and the other cup is backup for later use; and air vent holes are formed in the covers of the fermentation cups, and the covers cover cup openings. By adopting the household simple Tibetan kefir grain yogurt fermentation device disclosed by the invention, yogurt can be completely filtered in the fermentation cups under the condition that milk liquid is not poured out; and the household simple Tibetan kefir grain yogurt fermentation device cannot cause loss, is pollution-free, is simple to operate, is convenient and feasible, and is easy to popularize.
Owner:ANHUI SCI & TECH UNIV

Kefir whey and tea polyphenol composition as well as preparation method and application thereof

The invention belongs to the technical field of biotechnology, and discloses a kefir whey and tea polyphenol composition as well as a preparation method and application thereof. The method comprises the following operation steps: preparing a reduced milk solution from whole milk powder by using sterile water, sterilizing in a water bath, naturally cooling, adding kefir grains into the sterilized reduced milk under sterile operation, fermenting for 7-45 days at the constant temperature of 15-30 DEG C under a sealed condition, centrifuging, and filtering to obtain a kefir whey primary extracting solution; the preparation method comprises the following steps: preparing a Kefir whey working solution, carrying out reduced pressure evaporation and concentration to obtain a Kefir whey working solution, adding tea polyphenol into the Kefir whey working solution to prepare a solution with the mass percentage concentration of tea polyphenol being 0.05-0.5%, carrying out split charging, and carrying out water bath sterilization to obtain the Kefir whey and tea polyphenol composition. The composition has a very good effect of treating the inflammatory bowel disease, and the kefir whey and the tea polyphenol are derived from edible raw materials, so that the product is edible, high in safety, simple in production process and relatively low in cost.
Owner:SOUTH CHINA AGRI UNIV

Nut Kefir milk and preparation method thereof

The invention discloses nut Kefir milk and a preparation method thereof. The preparation method comprises a first step of preparing mother culture: first sterilizing nut juice and then adding leavening agent Kefir grains according to the proportion of 1:(20-50), performing fermentation for 24 h at a constant temperature of 18-22 DEG C, performing sieving, so that the filtered milk is the mother culture; a second step of preparing an operating leavening agent: taking another part of nut juice, adding the mother culture according to the proportion of 1%-3%; after uniform mixing, performing fermentation for 15-16 h at a constant temperature of 20-23 DEG C and then performing cooling to obtain the operating leavening agent; a third step of performing Kefir fermentation; first heating and homogenizing the nut juice, sterilizing the raw materials and then adding the prepared operating leavening agent into the raw materials according to the proportion of 2%-3%; after uniform mixing, performing constant-temperature fermentation for 10-12 h at 20-24 DEG C; after the fermentation is over, starting a stirring device to smash yogurt curd, and continuing to performing cooling to 14-16 DEG C, and causing the materials to stand for 12 h at this temperature and then performing after-ripening. According to the preparation method, the utilization of vegetable protein resources in the food industry is expanded fully.
Owner:上海银盏食品有限公司
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