The invention discloses a water injection well decompression and injection-augmentation agent containing biosurfactant, and relates to the technical field of oil exploitation auxiliaries. The water injection well decompression and injection-augmentation agent containing the biosurfactant is prepared from, by weight, 62-71 parts of lipopeptide biosurfactant, 22-31 parts of ampholytic surfactant, 3-5 parts of solubilizing assistant, 3-5 parts of clay stabilizer and 2-4 parts of scale inhibitor. According to the water injection well decompression and injection-augmentation agent containing the biosurfactant, wettability can be changed, oil-water interfacial tension is lowered to 10<-3> mN/m, in this way, starting of residual oil is facilitated, the phenomenon that the decompression and injection-augmentation agent causes emulsification to crude oil, so that water injection pressure rises can be avoided, the water injection well water injection pressure is effectively lowered, and the under injection well water injection capacity is improved; meanwhile, clay swelling can be prevented, and damage of carbonate precipitation such as calcium carbonate to stratum can be inhibited; the lipopeptide biosurfactant is adopted as the biosurfactant, and the water injection well decompression and injection-augmentation agent has the advantages of being environmentally friendly, low in dosage, good in water solubility, low in price and the like.