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Pseudomonas aeruginosa, and culture method and application thereof

A technology of Pseudomonas aeruginosa and cultivation methods, applied in chemical instruments and methods, microbiological-based methods, biochemical equipment and methods, etc., can solve the problem of small research fields, research lag, and restrictions on the application of Pseudomonas aeruginosa, etc. problems, to achieve the effects of expanded production, rapid growth and reproduction, improved water injection efficiency and oil recovery

Inactive Publication Date: 2012-07-04
RES INST OF SHAANXI YANCHANG PETROLEUM GRP
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Problems solved by technology

[0003] Pseudomonas aeruginosa has been studied more and can be used to produce biosurfactants, but the research field is relatively small, and the research on the production of surfactants used in oil fields to reduce the interfacial tension between oil and water is relatively lagging behind, which restricts the development of biosurfactants. Application of Pseudomonas aeruginosa in the field of oilfield exploitation

Method used

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  • Pseudomonas aeruginosa, and culture method and application thereof
  • Pseudomonas aeruginosa, and culture method and application thereof
  • Pseudomonas aeruginosa, and culture method and application thereof

Examples

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Embodiment Construction

[0022] The present invention will be described in detail below in combination with specific embodiments.

[0023] The Pseudomonas aeruginosa (Pseudomonas aeruginosa) YM4 involved in the present invention has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on September 9, 2011. The address of the preservation unit is No. 1 Beichen West Road, Chaoyang District, Beijing Court No. 3, the deposit number is CGMCC No.5243.

[0024] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the experimental materials used in the following examples, unless otherwise specified, are conventional biochemical reagents.

[0025] The medium involved in the following examples is:

[0026] Beef Extract Medium:

[0027] Peptone 1.0g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, 100mL water, pH 7.2-7.4.

[0028] Glucose Medium:

[0029] Glucose 2.0g, corn pulp 0.4g, sodium nitrate 0....

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Abstract

The invention relates to pseudomonas aeruginosa YM4 CGMCC No.5243, and a culture method and application thereof. The culture method for the pseudomonas aeruginosa YM4 comprises the following steps: inoculating the pseudomonas aeruginosa YM4 into a fermentation culture medium; and cultivating in a shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 or 80 rpm, wherein the formula of the fermentation culture medium comprises glucose, peptone, corn paste, sodium nitrate, disodium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride, and the pH value of the fermentation culture medium is 6.5 to 7.4. A biological surfactant generated by the pseudomonas aeruginosa YM4 can reduce oil water interface tension in the oil field; and after the action, the rheological property of the crude oil is obviously improved, viscosity is reduced, water injection pressure can be effectively reduced, water injection efficiency and recovery ratio of the crude oil can be improved; and large application prospect is achieved in the oil displacement process of the oil field.

Description

technical field [0001] The invention relates to a bacterial strain and its cultivation method and application, in particular to a strain of Pseudomonas aeruginosa and its cultivation method and application. Background technique [0002] Pseudomonas aeruginosa is a biosurfactant-producing bacterium that has been studied more in recent years. The rhamnolipid (RL) biosurfactant secreted by it has the functions of emulsifying, solubilizing, and reducing surface and interfacial tension, and has low toxicity and is easy to degrade in nature. Compared with synthetic surfactants, biosurfactants have the following properties Outstanding advantages: Raw materials are widely available in nature and cheap, and are not affected by oil prices. Fermentation production is a typical "green" process; the diversity of chemical structures provides a wider range of choices for specific applications, and these specific The group is generally difficult to synthesize by chemical methods, and at th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/02C09K8/582C09K8/584C12R1/385
Inventor 李世强王香增高瑞民赵丽薛媛梁凯强
Owner RES INST OF SHAANXI YANCHANG PETROLEUM GRP
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