The invention discloses a reactive dye colorfastness-to-bleeding improving agent, as well as a preparation method and application thereof. The reactive dye colorfastness-to-bleeding improving agent isprepared from the components: 50 to 70 parts of component A, and 30 to 50 parts of component B, wherein the component A comprises 10 to 30 parts of acrylamide, 5 to 10 parts of urea, 10 to 30 parts of methacrylic acid, 5 to 15 parts of stearic acid, 10 to 40 parts of polyethylene polyamine, 10 to 30 parts of distilled water, 5 to 15 parts of ammonium chloride, and 0.5 to 2 parts of initiator; thecomponent B comprises 5 to 10 parts of non-ionic surface active agent, 20 to 30 parts of quaternary ammonium salt, 40 to 80 parts of distilled water, and 1 to 5 parts of acidifier. The invention further provides the preparation method and the application of the reactive dye colorfastness-to-bleeding improving agent. According to the reactive dye colorfastness-to-bleeding improving agent, as wellas the preparation method and the application thereof provided by the invention, a dye is firmly fixed to the surface of a fabric, the adverse effect of metal ions on colorfastness to bleeding is effectively avoided, the surface tension of water is greatly reduced, the reactive dye is uniformly distributed on the fabric, a dyeing effect is good, the use performance is excellent, and the cost performance is high.