Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof

A technology of emulsification and wax fungicide, which is applied in the direction of cleaning utensils, drilling compositions, chemical instruments and methods, etc., and can solve the problems of slow paraffin speed, short contact time, and limited wax-proof effect

Active Publication Date: 2014-10-22
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two main deficiencies in this process: first, the microorganisms used in this technology are mainly bacteria that degrade crude oil, but the rate of microbial degradation of paraffin is relatively slow, and it usually takes 3 to 7 days to complete the degradation under laborat

Method used

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  • Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof
  • Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof
  • Strong emulsibility microbe wax cleaning and preventing bacterial agent and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] In the present embodiment, the strong emulsifying microbial anti-wax agent is composed of Pseudomonas aeruginosa N1 (CGMCC 1.2814), Pseudomonas aeruginosa N2 (CGMCC 1.10452) and surface bacillus (CGMCC 1.2673), 3 strains All were purchased from China General Microorganism Culture Collection Management Center (CGMCC). The bacterial agents are solid dry powders. The three bacteria are stored separately, and skim milk is used as the protective agent and carrier. The mass ratio of the three bacterial dry powders is 1:1:1. .

[0033] In the present embodiment, the application of the strong emulsifying microbial anti-wax agent in oil production comprises the following steps:

[0034] (1) Activate each strain separately until the cell concentration of each strain reaches 3.2~4.6×10 8 Each bacterial solution (2L) was mixed in equal volumes, and then the compound bacterial solution was added to the fermentation medium at a volume ratio of 1:10, fermented and cultivated at 40°C ...

Embodiment 2

[0040] In the present embodiment, the strong emulsifying microbial anti-wax agent is composed of Pseudomonas aeruginosa N1 (CGMCC 1.2814), Pseudomonas aeruginosa N2 (CGMCC 1.10452) and surface bacillus (CGMCC 1.2673), 3 strains All were purchased from China General Microorganism Culture Collection Management Center (CGMCC). The bacterial agents were in liquid state and stored separately. 8 individual / mL.

[0041] In the present embodiment, the application of the strong emulsifying microbial anti-wax agent in oil production comprises the following steps:

[0042] (1) Mix equal volumes of the bacteria liquids of various strains, transfer the compound bacterial liquids to the fermenter, add fermentation medium at a volume ratio of 1:10, ferment and cultivate at 35°C for 72 hours, and control the pH value at 6~ 8. There is no need to pass oxygen or air during the fermentation process. After the fermentation is completed, the next stage of fermentation is carried out. The volume r...

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Abstract

The invention discloses a strong emulsibility microbe wax cleaning and preventing bacterial agent and an application, which belongs to the oil field chemical technology field. The wax cleaning and preventing bacterial agent mainly comprises pseudomonas aeruginosa and geobacillus sp, has strong emulsification capability, and is capable of dispersing crude oil, promoting crude oil to flow, changing adherence force of borehole wall, preventing the accumulation of wax crystal on the borehole wall, and playing the paraffin inhibition and wax cleaning effects. By increasing the initial application amount of the bacteria liquid and prolong the well closing time, a stable paraffin inhibition zone can be formed due to adhesion of bacteria on borehole wall, near wellbore formation can be cleaned, and the paraffin inhibition and oil increase effects can be increased. After on-site enforcement, the well cleaning and chemical paraffin inhibition works can not be carried out in recent half year in the test wells, so that the wax cleaning and preventing bacterial agent has good paraffin inhibition effect in the well. The oil production is increased by about 30% daily, pump efficiency is increased about 10%, the surface tension of the output liquid is decreased to 20-25%, and the crude oil condensation point is decreased by 1-3 DEG C.

Description

technical field [0001] The invention specifically relates to a highly emulsifying microbial anti-wax agent and the application of the anti-wax agent, belonging to the technical field of oil field chemistry. Background technique [0002] During the crude oil extraction process, the wax in the crude oil is precipitated due to the reduction of pressure and temperature, forming crystals, which adhere to the near wellbore, wellbore and pump valves, etc., affecting the normal operation of the extraction and liquid extraction system. Periodic wax removal operations are required to remove wax crystals attached to the wellbore extraction system. The wax removal and prevention methods mainly used in oil fields include electric heating, hot water mixing, wax removal vehicles, chemical wax removal, and microbial wax removal and prevention methods. Compared with other methods, the microbial paraffin removal method has the advantages of energy saving, environmental protection, and no dam...

Claims

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Application Information

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IPC IPC(8): C09K8/524C09K8/582E21B37/06E21B43/22
CPCC09K8/524C09K8/582E21B37/06
Inventor 郝春雷苗建生常国栋王剑张红华王孟江
Owner CHINA PETROLEUM & CHEM CORP
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