The invention discloses a one-time well completion blasting method of a measure well. The method comprises following steps: firstly, trolley drilling, wherein a center hole and four empty holes are sequentially drilled in a lower chamber of the measure well upwards, four rings of charging holes are drilled from inside to outside, the center hole is located in the center position of the measure well, the empty holes are formed in the periphery of the center hole and are arranged in a square manner, the four empty holes are formed in the four top points of the square, the four rings of charging holes are sequentially provided with four, eight, nine, and fourteen charging holes from inside to outside, and each ring of charging holes are arranged on the circumference with the center hole as the round center at equal distances; secondly, charging, wherein emulsion explosives and detonators are loaded in the center hole and all the charging holes, and after charging, stemming is used for plugging hole openings; thirdly, blasting, wherein blasting is carried outside electronic detonator holes, and blasthole millisecond blasting is carried out. According to the one-time well completion blasting method of the measure well, the construction efficiency of the measure well is effectively improved, and the one-time well completion blasting effect and the wall face smoothness of the measure well are improved.