The present application relates to a kind of high stability water-in-oil PDRN
emulsion and its preparation method, belong to the field of
biotechnology;The preparation method includes the following steps: (1) PDRN enzymolysis: PDRN is added in DNaseI, supplement
metal ion activates
enzyme, when fragment enrichment shows <800bp, terminate enzymolysis reaction, obtain crude enzymolysis solution;(2) moderate molecular weight screening: pressurized
ultrafiltration crude enzymolysis solution with
ultrafiltration membrane, and screen out the target fragment of the permeate of molecular weight in 120-500bp;(3) water phase
system optimization: 1,3-
propanediol and ubiquinol are added in the permeate;(4) semi-
continuous feeding gradient emulsification.The present application is by precise control PDRN molecular weight to take into account stability and
solubility, optimize water phase component to realize
system viscosity and transparency double standard, cooperate gradient emulsification to guarantee water-in-oil
system stability, significantly improve product comprehensive quality and application value, with outstanding technical advantages.