Chitosan nanoparticle and preparation method and application thereof

A chitosan nanoparticle, chitosan technology, applied in the application, food preparation, pharmaceutical formulation and other directions, can solve the problems of large dose, long dissolution time, adverse reactions, etc., achieve small dose, simple and easy method, toxic Effects with few side effects

Inactive Publication Date: 2010-06-16
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically aiming at the disadvantages of the existing chitosan, such as large doses that are likely to cause adverse reactions and too long dissolution time in the stomach, a nano-sized chitosan is provided to improve the activity of chitosan and solve the problem of a

Method used

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  • Chitosan nanoparticle and preparation method and application thereof
  • Chitosan nanoparticle and preparation method and application thereof
  • Chitosan nanoparticle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 chitosan nanoparticles

[0046] (1) 25g chitosan (self-made or commercially available product according to the existing method, the reference molecular weight is 300,000 to 500,000, but the molecular weight has no direct relationship with the realization of the technology of the present invention, the same below) is dissolved in the acetic acid of 1000ml0.5% In the solution, stir to make it dissolve completely, and place it for later use;

[0047] (2) Add 100ml of 0.75mg / ml TPP to the chitosan solution in step (1) under the condition of mechanical stirring at room temperature and 800r / min, react for 45min, the system has obvious blue opalescence, and obtains nano-suspension liquid;

[0048] (3) The nanosuspension obtained in step (2) is concentrated by rotary evaporation at a temperature of 70° C., and the volume is evaporated to half of the volume of the nanosuspension;

[0049] (4) The concentrated solution obtained in step (3) is spr...

Embodiment 2

[0050] The preparation of embodiment 2 chitosan nanoparticles

[0051] (1) 25g chitosan is dissolved in the acetic acid solution that 1000ml volume ratio concentration is 1%, stirs to make it dissolve completely, place for subsequent use;

[0052](2) Add 0.85mg / ml TPP 100ml to the chitosan solution at room temperature and 600r / min mechanical stirring, and react for 60min. The system has obvious blue opalescence to generate a nanosuspension;

[0053] (3) The nanosuspension obtained in step (2) is concentrated by rotary evaporation at 60° C. under temperature control, and the volume is evaporated to half of the volume of the nanosuspension;

[0054] (4) The concentrated solution obtained in step (3) is spray-dried, the inlet temperature is set to 160°C, the outlet temperature is 85°C, the injection flow rate is 600ml / h, the pressure is 13atm, and the wind speed is 0.75m 3 / min. The resulting spray-dried chitosan nanoparticles.

Embodiment 3

[0055] The preparation of embodiment 3 chitosan nanoparticles

[0056] (1) 25g chitosan is dissolved in the acetic acid solution that 1000ml volume ratio concentration is 2%, stirs to make it dissolve completely, place for subsequent use;

[0057] (2) at room temperature, under the mechanical stirring condition of 600r / min, add 2mg / ml TPP 120ml to above-mentioned chitosan solution, react for 60min, the system has obvious blue opalescence generation, obtains nanosuspension;

[0058] (3) The nanosuspension obtained in step (2) is concentrated by rotary evaporation at a temperature of 75° C., and the volume is evaporated to half of the volume of the nanosuspension;

[0059] (4) The concentrated solution obtained in step (3) is spray-dried, the inlet temperature is set to 140°C, the outlet temperature is 110°C, the injection flow rate is 1000ml / h, the pressure is 5atm, and the wind speed is 0.65m 3 / min. The resulting spray-dried chitosan nanoparticles.

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Abstract

The invention discloses chitosan nanoparticle and a preparation method and application thereof. Chitosan and sodium tripolyphosphate are used to react and obtain the nanoparticle through rotary evaporation, concentration and spray drying. The invention adopts the simple and feasible method for the chitosan nanocrystallization, thus significantly increasing the activity of chitosan, solving the problem that chitosan is difficult to absorb and dissolve, greatly reducing the side effect and fundamentally resolving the current technical problems and technical bottleneck for losing weight and reducing blood fat of chitosan. The method of the invention is simple and feasible, has low cost and is easy to promote.

Description

technical field [0001] The invention belongs to the technical field of medicine and functional food, and in particular relates to a chitosan nanoparticle and a preparation method and application thereof. Background technique [0002] Chitosan (chitosan) is the N-deacetylation product of chitin, the chemical name is poly(1,4)-2-amino-2-deoxy -D-glucan. Widely present in the shells of lower biological fungi, algae cells, arthropod shrimp, crabs, and insects, it is the second largest biological polysaccharide after cellulose on the earth. Chitosan is a natural, non-toxic and biodegradable compound with excellent biocompatibility, antibacterial and adsorption properties, and has good adsorption properties for fat and cholesterol. [0003] The molecular structure of chitosan contains multiple amino groups. As a polycation, it can be closely combined with cholic acid and cholesterol with anionic groups in animals to form complexes. Such complexes are not easily hydrolyzed by ga...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/722A61P3/04A61P3/06A23L1/29A23L33/00
Inventor 苏政权张宏亮陶毅高冰胡因铭郭姣
Owner GUANGDONG PHARMA UNIV
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