34 results about "Mangiferin" patented technology

The invention provides a universal technical method for detecting non-toxic Chinese herbal medicine based on UPLC-QTOF-MS, and relates to the field of traditional Chinese medicine detection and analysis. The method comprises the following steps: S1, crushing non-toxic traditional Chinese medicinal materials; s2, preparing a to-be-detected sample solution; s3, preparing a QC accompanying standard product; s4, carrying out liquid mass analysis on the to-be-detected sample; and S5, processing mass spectrum information data. The method is characterized in that the QC standard substance in the step S3 is one or more of araflavin, aurantio-obtusin, patchouli ketone, apigenin, mangiferin, ginsenoside Rg1 and timosaponin, and chromatography-mass spectrometry conditions are defined in the step S4. The method disclosed by the invention has the advantages that a universal technical method is established instead of aiming at each specific traditional Chinese medicine, and targeted identification and analysis are not solved, so that a unified system is formed by inspection and analysis of different non-toxic Chinese herbal medicines, and non-targeted identification and analysis can be realized according to result differences on the premise of controlling the method differences.

Although compounds for treating or ameliorating malignant diseases have been proposed, patients had to endure a large burden since many of such compounds could not be orally ingested. Mangiferin has an effect of ameliorating malignant diseases through oral ingestion. However, such effect is minor and realistically it was not easy to ingest mangiferin. Further, in order to develop more effective pharmaceutical agents and the like for treating malignant diseases, it has been considered constantly necessary to obtain new or improved forms of an existing medical agent for inhibiting kinases such as NIK. A compound represented by formula (11) has an effect of ameliorating malignant diseases through oral ingestion, and can exhibit said effect with an amount less than that for mangiferin.

The invention discloses a pH and ROS double-response hydrogel based on probiotic microcapsules combined with prebiotics and a preparation method and application thereof, and belongs to the field of biomedical materials, the hydrogel is composed of the probiotic microcapsules, ROS response release mangiferin fragments and pH sensitive hydrogel; specifically, amino groups rich in the surface of the probiotic gelatin microcapsule are crosslinked with aldehyde groups contained in oxidized dextran and 4-formylphenylboronic acid to form a Schiff base bond with pH response. Mangiferin is combined with a boric acid group in 4-formylphenylboronic acid through a catechol structure and is crosslinked in the hydrogel to form a boric acid ester bond with ROS (reactive oxygen species) response. According to the hydrogel prepared by the preparation method disclosed by the invention, the probiotic microcapsules and the ROS responsively released prebiotics are combined, so that the synergistic antibacterial and antioxidant effects are achieved, and the effect of treating diabetic wounds is achieved.

The invention discloses a quantitative fingerprint spectrum analysis method for components in Da Yin-tonifying pills, and belongs to the field of quality control research of traditional Chinese medicines. The method comprises the following steps: preparing a reference solution and a test solution; injecting into a liquid chromatography for analysis and determination, selecting a Silversil C18 chromatographic column, carrying out gradient elution at the column temperature of 29.5-30.5 DEG C and the detection wavelength of 290 nm; and recording the chromatograms of the test solutions and the reference solutions of the Da Yin-tonifying pills in multiple batches, determining common peaks and quantitative detection indexes, and evaluating the quality of the Da Yin-tonifying pills according to the fingerprint similarity and the content of each component. The method can be used for determining 5-hydroxymethylfurfural, 3-O-feruloylquinic acid, neomangiferin and berberine hydrochloride in the Da Yin-tonifying pills, and has a wide application prospect in the field of quality control of the Da Yin-tonifying pills.

This invention discloses a traditional Chinese medicine composition for combating mandarin fish double RNA virus, comprising at least one of geniposide, baicalin, luteolin, magnolol, and mangiferin as active ingredients. The invention also discloses a method for preparing the traditional Chinese medicine composition and its applications. This invention can significantly inhibit the proliferation and replication of mandarin fish double RNA virus and significantly improve the survival rate of mandarin fish.

The invention discloses a strain of Chryseobacterium wanjuense C14, and a preparation method of the Chryseobacterium wanjuense C14. The invention further discloses application of the bacillus subtilis in inhibition of ralstonia solanacearum and application of the bacillus subtilis in degradation of straw. The Chryseobacterium sp. C14 can obviously inhibit the growth of pathogenic bacteria of tomato bacterial wilt, especially under the assistance of tomato rhizosphere metabolites such as O-phosphoethanolamine, mangiferin and L-tryptophan, the antibacterial effect of the Chryseobacterium sp. C14 is further enhanced, and the Chryseobacterium sp. C14 has higher environmental adaptability and prevention and treatment potential. The Chryseobacterium sp. C14 can also degrade protein, cellulose and hemicellulose at the same time, and can significantly promote the degradation of rice straw and corn straw. The Chryseobacterium sp. C14 has the functions of straw degradation and tomato ralstonia solanacearum inhibition, integrates a soil-borne disease biological control function and efficient straw degradation, and provides a green and efficient microbial new strategy for solving the problems of difficult straw treatment and difficult tomato bacterial wilt control in agricultural production.

This disclosure aims to provide a group of enzymes involved in the synthesis of nolatiliol from mangiferin. [Solution] The enzyme group of this disclosure includes: oxidoreductase DgmA and oxidoreductase DgmF which produce an intermediate (3-oxo-mangiferin) using mangiferin as a substrate; C-glucosidase complex DgmBC which produces nolatiliol using the intermediate (3-oxo-mangiferin) as a substrate; and hydratase DgmG which produces a cofactor (3-oxo-D-glucose) of oxidoreductase DgmA using a byproduct (1,5-anhydro-D-erythro-hexa-1-ene-3-urose) from the C-glucosidase complex DgmBC as a substrate.

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The present invention relates to a proprietary encapsulation technology where in a composition comprising mangiferin will maintain in-situ alkaline environment and minimizes the precipitation issues of mangiferin in the acidic environment. A composition of mangiferin has been disclosed. A natural hydrophilic plant-based carrier along with a selected alkalizer is being embedded in the encapsulation matrix which subsequently acts as a platform to ensure the alkaline environment with better solubility of bioactive across the physiological pH range.

The application belongs to the technical field of biological medicine, and particularly relates to a medium-chain fatty acid composition for improving immunity of calves and a preparation method thereof. The medium-chain fatty acid composition for improving immunity of calves according to the application comprises, in terms of weight fractions, 20-30 parts of lactose, 5-10 parts of medium-chain fatty acid, 1-3 parts of mangiferin derivative, 0.5-2 parts of wedelolactone and 0.2-1 part of vitamin C. The mangiferin derivative according to the application can relieve local inflammatory reaction of the intestinal tract, repair the intestinal mucosal barrier damaged by stress, and enhance the non-specific immune capacity of the body. The wedelolactone according to the application forms a double synergy with the lactose and the medium-chain fatty acid, greatly improves the utilization efficiency of energy and nutrients, and thus promotes the growth and development of the calf. In addition, the wedelolactone can also synergize with the mangiferin derivative, maintains the intestinal health of the calf in the early weaning period, and significantly weakens the weaning stress impact.

The invention belongs to the technical field of biomedical materials, and provides an intelligent hydrogel dressing with a three-layer structure as well as a preparation method and application of the intelligent hydrogel dressing. The intelligent hydrogel dressing with the three-layer structure comprises an upper layer, a middle layer and a bottom layer which are sequentially stacked, the upper layer is methacrylated gelatin hydrogel containing silver nanoparticles, the middle layer is methacrylated gelatin hydrogel containing mangiferin-loaded PLGA microspheres, and the bottom layer is methacrylated gelatin hydrogel. The upper layer of the intelligent hydrogel dressing with the three-layer structure is used for broad-spectrum antibiosis in the initial stage and quickly coping with bacterial infection; the middle layer is used for continuously releasing mangiferin, exerting the effects of resisting oxidation, resisting inflammation and promoting angiogenesis and eliminating oxidative stress; the bottom layer provides mechanical support and biocompatibility; through the synergistic effect of the multi-layer structure of the upper layer, the middle layer and the bottom layer, remarkable advantages are shown in multiple aspects such as mechanical support, medicine controllable release, broad-spectrum antibiosis and inflammation-oxidative stress regulation, and layering and cooperative intervention are achieved.

This invention discloses a bone repair scaffold material, its preparation method, and its application. The preparation method includes the following steps: preparation of a mineralized collagen core spinning solution; adding mangiferin to a PLGA electrospinning solution and stirring to obtain a shell spinning solution; and using the core spinning solution and the shell spinning solution, obtaining the bone repair scaffold material through coaxial electrospinning. In this invention, PLGA and mangiferin together form the shell of the scaffold structure, and the sustained release of mangiferin provides a guarantee for the anti-inflammatory and angiogenic effects of the scaffold material. Simultaneously, it simulates the fibrous structure of the bone matrix, achieving slow degradation of the core and achieving the goal of long-term promotion of bone repair.

The invention relates to a multi-antibacterial composite hydrogel dressing and a preparation method thereof, and the preparation method is characterized by comprising the following steps: (1) dissolving acrylamide and acrylic acid in water according to a mass ratio of 3: 7-7: 3; (2) sequentially adding 0.2 to 35 percent of vanadium trichloride and 0.09 to 15 percent of mangiferin, and uniformly stirring; (3) continuing to add 0.2-2% of N, N '-methylene bisacrylamide and 0.2-2% of ammonium persulfate, stirring and dissolving, adding 0.2-2% of N, N, N', N '-tetramethylethylenediamine, uniformly mixing, and injecting into a mold; (4) standing and reacting at room temperature for 6-48 hours to form tough brown hydrogel; and soaking and washing the brown hydrogel with deionized water for 3-5 times, and then cutting. The multi-antibacterial composite hydrogel dressing has the advantages that the antibacterial rate of the multi-antibacterial composite hydrogel dressing to staphylococcus aureus and escherichia coli is not lower than 70%, and the antibacterial rate can be increased to 80% or above under the assistance of near-infrared light.

The invention discloses a preparation method of a compound lysimachia christinae hance preparation extract, which comprises the following steps: carrying out water extraction and alcohol precipitation on traditional Chinese medicinal materials to obtain a crude extract, and purifying the crude extract with macroporous resin. By screening and optimizing extraction and purification processes, the purity of target components is effectively improved, the daily taking amount of extracts is reduced, and on the premise that the transfer rate is basically kept, compared with an existing preparation process of a compound herba lysimachiae preparation, the purity of total flavonoids, schaftoside and mangiferin is remarkably improved, and a huge space is provided for preparation of the preparation.

The invention relates to the technical field of medical biological materials, and discloses a hydrogel dressing for promoting wound healing and a preparation method and application thereof. Hyaluronic acid is modified through adipic dihydrazide to obtain modified hyaluronic acid, the modified hyaluronic acid and oxidized sodium alginate are subjected to a cross-linking reaction to form imine bonds, a hydrogel network is constructed, mangiferin (MGF) is directly loaded in the hydrogel network, and the hydrogel dressing capable of promoting wound healing is obtained. The hydrogel dressing can realize pH response drug release of the MGF through an imine bond formed by a cross-linking reaction. Under the acidic condition, the imine bond breaking speed in the hydrogel dressing is increased, meanwhile, more MGF is released, the hydrogel degradation speed is reduced for chronic wounds in an alkaline environment for a long time, at the moment, the MGF release speed can be effectively reduced, and therefore continuous treatment of the wounds is achieved.

The invention relates to the technical field of biological medicines, in particular to a synthesis method of a mangiferin-loaded liposome based on a microfluidic technology, the mangiferin-loaded liposome and application of the mangiferin-loaded liposome. The mangiferin-loaded liposome is synthesized by taking a mangiferin aqueous solution and a phospholipid-cholesterol organic solution as raw materials and using a micro-reactor with a micro-fluidic chip, so that high-flux and controllable synthesis of the liposome is realized, and the mangiferin-loaded liposome is suitable for industrial production and plays a positive role in treatment of arthritis.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The invention discloses a method for splitting xanthone C-C glucosidic bonds by using an enzyme method. The invention provides application of a protein combination A or a protein combination B, namely application in splitting C-C glucosidic bonds. The invention also relates to an application in converting mangiferin into mangiferin aglycone. Application in preparation of mangiferin aglycone; the invention also relates to application of the mangiferin in preparation of mangiferin aglycone. And the protein combination A comprises an LC1 protein, an LC2 protein and an LC3 protein. And the protein combination B is an LC4 protein, an LC5 protein and an LC6 protein. The method has important application and popularization values in the production field of mangiferin aglycone or mangiferin aglycone derivatives and downstream products thereof.

This invention belongs to the field of biomedical technology and discloses an α-hemolysin inhibitor and its application in the preparation of anti-infective drugs for Staphylococcus aureus. The inhibitor is mangiferin, and its novel use as a small molecule inhibitor of Staphylococcus aureus α-hemolysin (Hla) and related drug preparation methods are described. This invention, through in vitro hemolysis experiments, Hla protein purification and inhibitory activity verification, binding stability experiments, molecular docking studies, and in vivo animal experiments, confirms that mangiferin can efficiently and directly inhibit the hemolytic activity of Staphylococcus aureus supernatant and purified Hla protein in a dose-dependent manner; it can form a stable complex with Hla protein through hydrogen bonds and other interactions, blocking the pore-breaking activity of Hla in an irreversible or high-affinity binding manner, with a long-lasting inhibitory effect. This invention provides a novel candidate drug and technical solution for solving the problems of Staphylococcus aureus (including drug-resistant strains) infection and antibiotic resistance, with broad application prospects.

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a peach-astragalus hematopoiesis capsule as well as construction and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and formononin, formononetin, calycosin 7-O-beta-D-glucoside, calycosin, ferulic acid, rutin and 5-hydroxymethylfurfural are used as reference substances; and the fingerprint spectrum is successfully constructed by detecting different batches of the peach-astragalus hematopoiesis capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least seven components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be accurately, rapidly and simultaneously carried out on a plurality of characteristic components of the peach-stilbene hematogenic capsule, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the peach-stilbene hematogenic capsule.

This invention belongs to the food field, specifically health food, and discloses a Euglena composition for improving gout and its preparation method. The composition is prepared from the following raw materials in parts by weight: 3-8 parts of Coix seed, 3-8 parts of Poria cocos, 0.1-6 parts of Gardenia jasminoides, 0.1-6 parts of Imperata cylindrica root, 2-5 parts of corn silk, 2-5 parts of Pueraria lobata root, 0.1-10 parts of Euglena galanga powder, 0.1-5 parts of Lactobacillus plantarum powder, 0.1-4 parts of mangiferin, 0.1-6 parts of celery seed extract, and 0.1-5 parts of sour cherry extract. The preparation process is efficient and controllable, and the application is safe and effective.

The application discloses a mango glycoside liquor with effects of dispelling alcohol and a preparation method thereof. The liquor is prepared by adding a specific content of mango glycoside, and thus the effects of preventing drunkenness and dispelling alcohol are achieved.

The invention discloses an HPLC (High Performance Liquid Chromatography) specific chromatogram of Baifeng oral liquid as well as a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, a high performance liquid chromatography method is adopted, and guanosine, gallic acid, gentiopicroside, oxypaeoniflorin, chlorogenic acid, gentiopicroside, albiflorin, paeoniflorin and mangiferin are taken as reference substances; different batches of Baifeng oral liquids are detected, and the characteristic chromatogram is successfully constructed. The method is simple and easy to implement, good in precision, high in stability and good in reproducibility, at least nine components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; according to the method, the quality of the 18 characteristic components of the Baifeng oral liquid can be accurately and rapidly monitored at the same time, the stability and uniformity of the product quality can be controlled, the safety and effectiveness of clinical medication are ensured, and a reference is provided for quality control and clinical application of the Baifeng oral liquid.

The application discloses a content determination method of a traditional Chinese medicine composition containing Ramulus Cinnamomi, and the method comprises the following steps: detecting test sample solution and control sample solution of the traditional Chinese medicine composition; and obtaining content information of the control sample of the traditional Chinese medicine composition according to the detection result; wherein the traditional Chinese medicine composition comprises Ramulus Cinnamomi, Radix Paeoniae Alba, Radix Glycyrrhizae, Ephedra, Rhizoma Zingiberis Recens, Rhizoma Atractylodis Macrocephalae, Rhizoma Anemarrhenae, Radix Saposhnikoviae and Heishunpian; and the control sample comprises Mangiferin, Paeonoside, Anemonin, 5-O-Methylvisamminol, Cinnamyl Aldehyde, Ammonium Glycyrrhizate and 6-Gingerol. The detection method is simple, accurate and efficient, has good stability, high precision, good reproducibility, is convenient and easy to master, can effectively save energy and reduce analysis cost, and the technical content is not disclosed in the prior art.

The invention discloses mangiferin / vanadium / PVP (Polyvinyl Pyrrolidone) composite nanoparticles as well as a preparation method and application thereof, and belongs to the field of nano medical materials. The composite nano particle disclosed by the invention is formed by self-assembling mangiferin, vanadium trichloride hexahydrate and polyvinyl pyrrolidone under the coordination action. The preparation method is simple and convenient, conditions are mild, the prepared nanoparticles are uniform in particle size, have good water dispersibility and photothermal conversion performance and have inherent bacteriostatic activity on various bacteria, and the antibacterial effect can be synergistically enhanced under irradiation of near-infrared light. The material has application prospects in the biomedical fields of antibiosis, photo-thermal therapy preparations and the like.

The invention provides a method for detecting mangiferin and derivatives thereof. The method comprises the following steps: (1) sample pretreatment; (2) gradient elution separation; and (3) mass spectrum detection. By optimizing sample pretreatment, chromatographic separation and mass spectrometry detection conditions, the invention establishes a high-sensitivity and high-selectivity detection method for mangiferin and derivatives thereof.

The application discloses a kind of fifteen kinds of sereel Douwan fingerprint detection methods, it includes the following steps: a, preparation of reference solution: taking gallic acid, magnolia base, mangiferin, ellagic acid, berberine hydrochloride reference, add methanol to dissolve, namely obtained;B, preparation of test solution: take fifteen kinds of sereel Douwan, add methanol solution extraction, filter, take the filtrate, namely obtained;C, respectively, reference solution and test solution are pumped into liquid chromatograph and detected.The method first establishes the fingerprint of fifteen kinds of sereel Douwan, provides simple and accurate method for the detection and identification of fifteen kinds of sereel Douwan, guarantees the quality of fifteen kinds of sereel Douwan, also provides scientific basis for its clinical application, with good application prospect.

The invention provides injectable hydrogel as well as a preparation method and application thereof in treatment of periodontitis, epigallocatechin gallate powder is dissolved in a NaHCO3 solution and stirred in a dark place to be uniformly dispersed; the preparation method comprises the following steps: dissolving mangiferin powder into an epigallocatechin gallate solution, uniformly stirring and mixing, putting into a magnetic water bath kettle, uniformly stirring and mixing until gelation, adding a NaOH solution, and gradually changing the gel into semitransparent gel, wherein the temperature of the magnetic water bath kettle is 65-75 DEG C; the hydrogel with antioxidant and anti-inflammatory activity does not simply depend on loaded drugs. The hydrogel capable of long-acting and slowly releasing therapeutic drugs is provided, the local effective concentration is maintained, and the administration frequency is reduced.

The application discloses an allicol oligosaccharide as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Alkaline phosphatase staining and alizarin red staining tests prove that the allicol oligosaccharide AMO-1 can significantly improve the activity level of alkaline phosphatase in MC3T3 cells and osteogenic mineralization nodules, and can significantly up-regulate the expression levels of osteogenic differentiation related proteins RUNX2, OSX, OCN and COL1; animal experiments prove that AMO-1 can obviously relieve bone loss of DEX-induced osteoporosis mice, and can be used for preparing medicines for preventing and treating osteoporosis. The oligosaccharide provided by the application has high purity, does not contain impurities such as proteins and nucleic acids, has small toxic and side effects, has a wide application prospect in preparation of low-toxicity and high-efficiency anti-osteoporosis medicines, and is favorable for further development and utilization of allicol resources.