57 results about "Mangiferin" patented technology

The invention discloses a method for determining mangiferin in a mango extract, and belongs to the technical field of plant detection. The determination method comprises the following steps: firstly, ionizing sample ions from a target material, enabling at least part of the sample ions to enter a mobility pool, capturing the sample ions to generate mass spectrum data, and extracting first sampling data and second sampling data from the mass spectrum data; according to the method, the mangiferin and other xanthone derivatives in the mango extract are separated according to a mobility pool, and then the content of the mangiferin is determined through flight mass spectrometry. According to the method, the migration interval of the to-be-detected component is adjusted according to the actually measured signal peak, the corresponding sampling data is intercepted from the migration interval, and then the electric field parameters are adjusted, so that the signal peak interval is prevented from being too small, the mangiferin and other xanthone derivatives can be distinguished, and the testability of the sampling data is ensured.

The invention discloses a mangiferin derivative, a preparation method and application of the mangiferin derivative in preparation of weight-reducing drugs. The mangiferin derivative is simple to prepare, has a very good inhibition effect on liposynthase, has an inhibition effect on 3T3-L1 fat cell proliferation, and has a prospect of preparing obesity treatment drugs.

The invention discloses application of mangiferin in preparation of a protective agent for testicular sertoli cells and preparation of a medicine for treating male infertility diseases, and relates to the technical field of medicine application. Wherein the mangiferin can play a role in protecting the sertoli cells, and the protection role is reflected in ways of improving the cell viability of the sertoli cells, inhibiting the apoptosis of the sertoli cells, inhibiting the oxidative stress of the sertoli cells, inhibiting the inflammatory response of the sertoli cells and the like; meanwhile, mangiferin can be used for treating male infertility diseases in ways of relieving weight loss, repairing testis, improving endocrine system functions, inhibiting oxidative stress of testis tissues, inhibiting inflammatory response of the testis tissues and the like. Wherein the mangiferin can inhibit the inflammatory reaction mainly by inhibiting the activation of NLRP3 inflammasome to inhibit the NLRP3 pathway, so that the inflammation and even pyroptosis of testicular sertoli cells are remarkably reduced to improve spermatogenesis, and the male infertility disease is well treated.

The invention belongs to the technical field of medical application, and particularly relates to application of neomangiferin in preparation of an anti-depression drug, experiments find that the effect of neomangiferin is obviously better than that of mangiferin, although the neomangiferin and the mangiferin have the same main structure, the inventor finds that the effects of the neomangiferin and the mangiferin have obvious difference, and the effect of the neomangiferin and the mangiferin is obviously different. The compound is unexpected by the inventor before experiments, a new optional medicine is provided for research and development of anti-depression, and the effect of the anti-depression medicine is effectively improved.

The invention discloses preparation of an anti-chronic pharyngitis microcapsule medicine and application of the anti-chronic pharyngitis microcapsule medicine in tipping paper for cigarettes, the medicine is MF-GA / IL (at) MPN, and the medicine is composed of an ionic liquid (IL) as an internal carrier and a microcapsule carrier with a metal phenol network (MPN) as a shell; wherein the metal phenol network is formed by aluminum ions and mangiferin through a coordination reaction, and the ionic liquid is CAGE ILs formed by vanillin and choline. The combination of mangiferin and glycyrrhizic acid is used as a strategy for treating chronic pharyngitis, it is found that the effective loaded MF-GA / IL-coated MPN microcapsule preparation is obtained by packaging the two drugs including mangiferin and glycyrrhizic acid with the ionic liquid as the core and the metal phenol network as the shell, the tissue permeability of the drugs is improved, a certain slow release effect is achieved, and the effect of treating chronic pharyngitis is achieved. The bioavailability of mangiferin and glycyrrhizic acid is improved, and the anti-inflammatory activity effect is better achieved.

The invention relates to a pharmaceutical composition, the pharmaceutical composition contains an effective amount of active ingredients and pharmaceutically acceptable auxiliary materials, and the active ingredients comprise mangiferin, glycyrrhizic acid and cinnamic acid. The invention also relates to an application of the pharmaceutical composition in preparation of a medicine for treating or improving diseases, wherein the diseases are rheumatoid arthritis; the pharmaceutical composition contains an effective amount of active ingredients, and the active ingredients comprise mangiferin, glycyrrhizic acid and cinnamic acid. The pharmaceutical composition disclosed by the invention can be used for remarkably improving the severity of rheumatoid arthritis of a rat suffering from adjuvant-induced rheumatoid arthritis. And almost no adverse reaction or side effect exists.

The application discloses application of mangiferin as a broad-spectrum inhibitor of active carbonyl compounds, and provides application of mangiferin (MGF) as an acrolein (ACR), methyl glyoxal (MGO) and glyoxal (GO) inhibitor, wherein, compared with the prior art, the application discloses a new application of mangiferin or a one-addition product of the mangiferin and the active carbonyl compound, that is, the mangiferin or the one-addition product can effectively capture ACR, MGO and GO to control the content of the ACR, MGO and GO and avoid the ACR, MGO and GO from reacting with nucleophilic biological macromolecules to form various harmful addition or cross-linking products. The mangiferin or the one-addition product of the mangiferin and the active carbonyl compound can be used as a scavenger of ACR, MGO and GO, so that the ACR, MGO and GO generated in the body and in a food processing process are inhibited, and thus the various harmful addition or cross-linking products formed by the reaction of RCS with the nucleophilic biological macromolecules are blocked to cause harm to the human body.

The invention provides a universal technical method for detecting non-toxic Chinese herbal medicine based on UPLC-QTOF-MS, and relates to the field of traditional Chinese medicine detection and analysis. The method comprises the following steps: S1, crushing non-toxic traditional Chinese medicinal materials; s2, preparing a to-be-detected sample solution; s3, preparing a QC accompanying standard product; s4, carrying out liquid mass analysis on the to-be-detected sample; and S5, processing mass spectrum information data. The method is characterized in that the QC standard substance in the step S3 is one or more of araflavin, aurantio-obtusin, patchouli ketone, apigenin, mangiferin, ginsenoside Rg1 and timosaponin, and chromatography-mass spectrometry conditions are defined in the step S4. The method disclosed by the invention has the advantages that a universal technical method is established instead of aiming at each specific traditional Chinese medicine, and targeted identification and analysis are not solved, so that a unified system is formed by inspection and analysis of different non-toxic Chinese herbal medicines, and non-targeted identification and analysis can be realized according to result differences on the premise of controlling the method differences.

Although compounds for treating or ameliorating malignant diseases have been proposed, patients had to endure a large burden since many of such compounds could not be orally ingested. Mangiferin has an effect of ameliorating malignant diseases through oral ingestion. However, such effect is minor and realistically it was not easy to ingest mangiferin. Further, in order to develop more effective pharmaceutical agents and the like for treating malignant diseases, it has been considered constantly necessary to obtain new or improved forms of an existing medical agent for inhibiting kinases such as NIK. A compound represented by formula (11) has an effect of ameliorating malignant diseases through oral ingestion, and can exhibit said effect with an amount less than that for mangiferin.

The invention discloses a pH and ROS double-response hydrogel based on probiotic microcapsules combined with prebiotics and a preparation method and application thereof, and belongs to the field of biomedical materials, the hydrogel is composed of the probiotic microcapsules, ROS response release mangiferin fragments and pH sensitive hydrogel; specifically, amino groups rich in the surface of the probiotic gelatin microcapsule are crosslinked with aldehyde groups contained in oxidized dextran and 4-formylphenylboronic acid to form a Schiff base bond with pH response. Mangiferin is combined with a boric acid group in 4-formylphenylboronic acid through a catechol structure and is crosslinked in the hydrogel to form a boric acid ester bond with ROS (reactive oxygen species) response. According to the hydrogel prepared by the preparation method disclosed by the invention, the probiotic microcapsules and the ROS responsively released prebiotics are combined, so that the synergistic antibacterial and antioxidant effects are achieved, and the effect of treating diabetic wounds is achieved.

The invention discloses a method for simultaneously determining the contents of baohuoside, mangiferin, kaempferol, timosaponin A III and timosaponin B II in rhizoma anemarrhenae based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. The method is based on an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, a methanol organic phase-0.1% formic acid water phase system is used as a mobile phase, a Waters Acquity UPLCBEH C18 column is selected, ESI is used as a mass spectrometry detection ion source, and the contents of the five traditional Chinese medicines in the rhizoma anemarrhenae powder are accurately quantified at the same time in a positive and negative ion synchronous scanning and multi-reaction monitoring mode. The method has the advantages of simple pretreatment, high detection sensitivity and good reproducibility, and can be used for identification or quality control of main components of corresponding traditional Chinese medicines.

The invention discloses a quantitative fingerprint spectrum analysis method for components in Da Yin-tonifying pills, and belongs to the field of quality control research of traditional Chinese medicines. The method comprises the following steps: preparing a reference solution and a test solution; injecting into a liquid chromatography for analysis and determination, selecting a Silversil C18 chromatographic column, carrying out gradient elution at the column temperature of 29.5-30.5 DEG C and the detection wavelength of 290 nm; and recording the chromatograms of the test solutions and the reference solutions of the Da Yin-tonifying pills in multiple batches, determining common peaks and quantitative detection indexes, and evaluating the quality of the Da Yin-tonifying pills according to the fingerprint similarity and the content of each component. The method can be used for determining 5-hydroxymethylfurfural, 3-O-feruloylquinic acid, neomangiferin and berberine hydrochloride in the Da Yin-tonifying pills, and has a wide application prospect in the field of quality control of the Da Yin-tonifying pills.

This invention discloses a traditional Chinese medicine composition for combating mandarin fish double RNA virus, comprising at least one of geniposide, baicalin, luteolin, magnolol, and mangiferin as active ingredients. The invention also discloses a method for preparing the traditional Chinese medicine composition and its applications. This invention can significantly inhibit the proliferation and replication of mandarin fish double RNA virus and significantly improve the survival rate of mandarin fish.

The invention discloses a strain of Chryseobacterium wanjuense C14, and a preparation method of the Chryseobacterium wanjuense C14. The invention further discloses application of the bacillus subtilis in inhibition of ralstonia solanacearum and application of the bacillus subtilis in degradation of straw. The Chryseobacterium sp. C14 can obviously inhibit the growth of pathogenic bacteria of tomato bacterial wilt, especially under the assistance of tomato rhizosphere metabolites such as O-phosphoethanolamine, mangiferin and L-tryptophan, the antibacterial effect of the Chryseobacterium sp. C14 is further enhanced, and the Chryseobacterium sp. C14 has higher environmental adaptability and prevention and treatment potential. The Chryseobacterium sp. C14 can also degrade protein, cellulose and hemicellulose at the same time, and can significantly promote the degradation of rice straw and corn straw. The Chryseobacterium sp. C14 has the functions of straw degradation and tomato ralstonia solanacearum inhibition, integrates a soil-borne disease biological control function and efficient straw degradation, and provides a green and efficient microbial new strategy for solving the problems of difficult straw treatment and difficult tomato bacterial wilt control in agricultural production.

This disclosure aims to provide a group of enzymes involved in the synthesis of nolatiliol from mangiferin. [Solution] The enzyme group of this disclosure includes: oxidoreductase DgmA and oxidoreductase DgmF which produce an intermediate (3-oxo-mangiferin) using mangiferin as a substrate; C-glucosidase complex DgmBC which produces nolatiliol using the intermediate (3-oxo-mangiferin) as a substrate; and hydratase DgmG which produces a cofactor (3-oxo-D-glucose) of oxidoreductase DgmA using a byproduct (1,5-anhydro-D-erythro-hexa-1-ene-3-urose) from the C-glucosidase complex DgmBC as a substrate.

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The present invention relates to a proprietary encapsulation technology where in a composition comprising mangiferin will maintain in-situ alkaline environment and minimizes the precipitation issues of mangiferin in the acidic environment. A composition of mangiferin has been disclosed. A natural hydrophilic plant-based carrier along with a selected alkalizer is being embedded in the encapsulation matrix which subsequently acts as a platform to ensure the alkaline environment with better solubility of bioactive across the physiological pH range.

The application belongs to the technical field of biological medicine, and particularly relates to a medium-chain fatty acid composition for improving immunity of calves and a preparation method thereof. The medium-chain fatty acid composition for improving immunity of calves according to the application comprises, in terms of weight fractions, 20-30 parts of lactose, 5-10 parts of medium-chain fatty acid, 1-3 parts of mangiferin derivative, 0.5-2 parts of wedelolactone and 0.2-1 part of vitamin C. The mangiferin derivative according to the application can relieve local inflammatory reaction of the intestinal tract, repair the intestinal mucosal barrier damaged by stress, and enhance the non-specific immune capacity of the body. The wedelolactone according to the application forms a double synergy with the lactose and the medium-chain fatty acid, greatly improves the utilization efficiency of energy and nutrients, and thus promotes the growth and development of the calf. In addition, the wedelolactone can also synergize with the mangiferin derivative, maintains the intestinal health of the calf in the early weaning period, and significantly weakens the weaning stress impact.

The present invention discloses a method and application thereof for analyzing the active ingredients of Radix Trichosanthis for treating acute pancreatitis based on HPLC-Q-TOF-MS, network pharmacology, and molecular docking. The analysis method uses HPLC-Q-TOF-MS / MS analysis technology to generate a molecular network of chemical components through accurate mass number, secondary mass spectrometry data, mass spectrometry fragmentation rules, and a database. Then, using a network pharmacology-related database, acute pancreatitis disease-related targets are obtained, a "component-disease-target" network diagram is constructed, a protein interaction network is constructed, and GO function and KEGG pathway enrichment analysis are performed. The 10 key chemical components obtained are: quercetin, coumarin glucoside, gentiopicroside, eriodictyol-7-O-glucoside, protocatechuic acid, 3-O-caffeoylquinic acid, astragalin, isoorientin, isovitexin, and mangiferin.

The present invention discloses a method for determining mangiferin in a mango extract, and belongs to the technical field of plant detection. The determination method first ionizes sample ions from a target material, and at least part of the sample ions enter a mobility cell. After capturing the sample ions, mass spectrum data is generated, and first sampling data and second sampling data are extracted from the mass spectrum data. The present invention separates mangiferin and other xanthone derivatives in the mango extract based on the mobility cell, and then determines the content of mangiferin by flight mass spectrometry. The present invention adjusts the migration interval where the component to be measured is located according to the measured signal peak, intercepts the corresponding sampling data from the migration interval, and then adjusts the electric field parameters to avoid too small a signal peak spacing, so as to distinguish mangiferin from other xanthone derivatives and ensure the measurability of the sampling data.

The present application relates to the field of embryo engineering, and particularly relates to application of mangiferin in promoting maturation of animal oocytes. The present application discloses that mangiferin can promote maturation of animal oocytes, especially maturation of pig oocytes; can improve in-vitro maturation efficiency of oocytes; can reduce the apoptosis rate of oocytes and improve the subsequent development potential of oocytes. Mangiferin can be used to promote in-vitro maturation of oocytes, and can be used to prepare or directly serve as a drug for improving in-vitro maturation and development of oocytes, thereby opening up a new application direction for mangiferin. The present application uses mangiferin to improve in-vitro maturation efficiency of oocytes, solves the problems of low maturation efficiency and long culture period, and provides more high-quality oocytes for somatic cell nuclear transfer, in-vitro fertilization and transgenic cloning by using the technical scheme of mangiferin in the present application, thereby having a wide application prospect in actual production.

The invention belongs to the technical field of biomedical materials, and provides an intelligent hydrogel dressing with a three-layer structure as well as a preparation method and application of the intelligent hydrogel dressing. The intelligent hydrogel dressing with the three-layer structure comprises an upper layer, a middle layer and a bottom layer which are sequentially stacked, the upper layer is methacrylated gelatin hydrogel containing silver nanoparticles, the middle layer is methacrylated gelatin hydrogel containing mangiferin-loaded PLGA microspheres, and the bottom layer is methacrylated gelatin hydrogel. The upper layer of the intelligent hydrogel dressing with the three-layer structure is used for broad-spectrum antibiosis in the initial stage and quickly coping with bacterial infection; the middle layer is used for continuously releasing mangiferin, exerting the effects of resisting oxidation, resisting inflammation and promoting angiogenesis and eliminating oxidative stress; the bottom layer provides mechanical support and biocompatibility; through the synergistic effect of the multi-layer structure of the upper layer, the middle layer and the bottom layer, remarkable advantages are shown in multiple aspects such as mechanical support, medicine controllable release, broad-spectrum antibiosis and inflammation-oxidative stress regulation, and layering and cooperative intervention are achieved.

This invention discloses a bone repair scaffold material, its preparation method, and its application. The preparation method includes the following steps: preparation of a mineralized collagen core spinning solution; adding mangiferin to a PLGA electrospinning solution and stirring to obtain a shell spinning solution; and using the core spinning solution and the shell spinning solution, obtaining the bone repair scaffold material through coaxial electrospinning. In this invention, PLGA and mangiferin together form the shell of the scaffold structure, and the sustained release of mangiferin provides a guarantee for the anti-inflammatory and angiogenic effects of the scaffold material. Simultaneously, it simulates the fibrous structure of the bone matrix, achieving slow degradation of the core and achieving the goal of long-term promotion of bone repair.

The invention discloses a targeted delivery preparation as well as a preparation method and application thereof, and belongs to the technical field of biology and new medicine. The targeted delivery preparation comprises an agent A and an agent B in a ratio of (0.1-0.3) g: (2-5) mL; the agent A comprises nasal brain targeting peptide modified stem cell factor liposome freeze-dried powder and gel dry powder, the amino acid sequence of the nasal brain targeting peptide is as shown in SEQ ID NO.1, and the gel dry powder is formed by self-crosslinking mangiferin and rutin; and the agent B is a normal saline solution containing carboxymethyl chitosan. The targeted delivery preparation not only can meet long-time stable storage, but also can enhance the nasal-brain delivery efficiency of stem cell drugs. Besides, the targeted delivery preparation can be used independently or in combination with other treatment drugs, and is used for treating ischemic cerebral diseases, especially cerebral arterial thrombosis.

The invention relates to a multi-antibacterial composite hydrogel dressing and a preparation method thereof, and the preparation method is characterized by comprising the following steps: (1) dissolving acrylamide and acrylic acid in water according to a mass ratio of 3: 7-7: 3; (2) sequentially adding 0.2 to 35 percent of vanadium trichloride and 0.09 to 15 percent of mangiferin, and uniformly stirring; (3) continuing to add 0.2-2% of N, N '-methylene bisacrylamide and 0.2-2% of ammonium persulfate, stirring and dissolving, adding 0.2-2% of N, N, N', N '-tetramethylethylenediamine, uniformly mixing, and injecting into a mold; (4) standing and reacting at room temperature for 6-48 hours to form tough brown hydrogel; and soaking and washing the brown hydrogel with deionized water for 3-5 times, and then cutting. The multi-antibacterial composite hydrogel dressing has the advantages that the antibacterial rate of the multi-antibacterial composite hydrogel dressing to staphylococcus aureus and escherichia coli is not lower than 70%, and the antibacterial rate can be increased to 80% or above under the assistance of near-infrared light.

The present invention discloses a new use of a plant-derived glycoside compound, relating to the fields of pesticides and plant protection. The plant-derived glycoside compound is galangin or isomandibolic acid. At the same time, the present invention discloses an agent for resisting tobacco mosaic virus or preventing and controlling crop diseases, comprising the plant-derived glycoside compound as described above. During treatment, an effective amount of the plant-derived glycoside compound can be applied, sprayed, or injected onto tobacco leaves or crops. The present invention first discovered that galangin and isomandibolic acid have good anti-TMV activity, wherein the anti-TMV activity of galangin reaches 82.22%, and the anti-TMV activity of isomandibolic acid reaches 75.00%, providing a material basis for anti-TMV research; molecular docking technology is used to simulate the binding mode of galangin and isomandibolic acid with target proteins, and three potential targets of galangin and isomandibolic acid are screened out based on the minimum binding energy and the number of hydrogen bonds, providing a theoretical basis for anti-TMV research.

The invention discloses a method for calculating the content range of mangiferin in mango kernels and application. The method comprises the following steps: randomly sampling the mango kernels from batches of to-be-detected mango kernels, breaking shells of the mango kernels, taking out internal seeds, grouping the shells and the seeds of the mango kernels, and calculating the weight of the shells and the weight of the seeds in each group; respectively pulverizing and sieving the shells and the seeds according to groups, detecting the mangiferin content in each group of shells and the mangiferin content in the seeds, and calculating the theoretical mangiferin content of each group of mango kernels; and calculating the mangiferin content range in the to-be-detected batch of mango kernels according to the theoretical mangiferin content of each group of mango kernels. The method can accurately calculate the range of the mangiferin content in the mango seeds, can accurately judge whether the content of the batch of medicinal materials meets the internal control standard or not by comparing whether the lower limit value of the content range is higher than the internal control standard requirement of the medicinal materials or not, avoids the situation that the medicinal materials with unqualified content are misjudged to be qualified, and reduces the risk that the medicinal materials are qualified.

The invention provides a fluorescence recognition method of mangiferin on Zn and Cu, and belongs to the technical field of fluorescence recognition, the method comprises the following steps: probe MF spectrum performance test, probe MF selective recognition experiment on metal ions, fluorescence titration experiment, interference experiment, and equal substance amount continuous change experiment. The invention discloses the excellent fluorescence characteristic of the mangiferin, the fluorescence intensity of the mangiferin (MF) can be obviously enhanced by adding Zn < 2 + > in a neutral aqueous solution, and the fluorescence of the MF is obviously weakened by adding Cu < 2 + >. The concentrations of the two metal ions and the fluorescence intensity of the system have a good linear relationship, and the detection limits of the two metal ions respectively reach 0.449 mu mol / L and 2.27 mu mol / L, which are lower than the detection limits reported in many literatures, so that the two metal ions are highly sensitive. An interference experiment result further shows that coexistence of other metal ions has no obvious interference on identification of Zn < 2 + > and Cu < 2 + > by MF.

The invention discloses a preparation method of a compound lysimachia christinae hance preparation extract, which comprises the following steps: carrying out water extraction and alcohol precipitation on traditional Chinese medicinal materials to obtain a crude extract, and purifying the crude extract with macroporous resin. By screening and optimizing extraction and purification processes, the purity of target components is effectively improved, the daily taking amount of extracts is reduced, and on the premise that the transfer rate is basically kept, compared with an existing preparation process of a compound herba lysimachiae preparation, the purity of total flavonoids, schaftoside and mangiferin is remarkably improved, and a huge space is provided for preparation of the preparation.