11 results about "Chryseobacterium sp." patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to Chryseobacterium sp. 2R14A and application of the Chryseobacterium sp. 2R14A. The strain is excellent in oxidation resistance, still can grow after being treated by 80mM hydrogen peroxide for 30 minutes, and is not completely inactivated after being treated by 160mM hydrogen peroxide, and a genome contains antioxidant related genes; growth conditions are mild, sodium chloride is not needed, large-scale culture is easy, cost is low, and physiological and biochemical characteristics are stable. The strain can be used for treating oxidizing agent-containing polluted environment and improving stress resistance of crops or microorganisms, can also be used as a gene engineering recipient bacterium or a mutation breeding material, and expands application in the fields of agriculture, environment and medicine.

The invention relates to the field of antibiotic degradation, in particular to an aminoglycoside antibiotic degradation microbial inoculum as well as a preparation method and application thereof. The aminoglycoside antibiotic degrading bacterial agent is prepared from the following raw materials: a seed solution of Chryseobacterium (Chryseobacterium sp.) (Chryseobacterium Zn-C) and iron lanthanum modified hydrothermal carbon. The preparation method comprises the following steps: (1) pretreating straws by using a ferric chloride and lanthanum chloride composite solution, and carrying out hydrothermal carbonization; (2) preparing a Chryseobacterium (Chryseobacterium sp.) (Chryseobacterium Zn-C seed solution, and preparing a seed solution of the Chryseobacterium (Chryseobacterium sp.) (Chryseobacterium Zn-C); and (3) compounding the iron-lanthanum modified hydrothermal carbon with the seed solution to form the aminoglycoside antibiotic degrading microbial inoculum. The microbial agent is put into a water body, soil or compost polluted by the aminoglycoside antibiotics to degrade the aminoglycoside antibiotics so as to achieve the pollution remediation effect.

The present invention relates to the technical field of genetic engineering, and specifically relates to a ketoreductase mutant and the use thereof. The ketoreductase mutant has one or more amino acid mutations as compared to the amino acid sequence of a wild-type ketoreductase as shown in SEQ ID NO. 2, wherein the wild-type ketoreductase is derived from Chryseobacterium sp. When a ketoreductase and a mutant thereof are used in the resolution of a mirogabalin intermediate, the chiral purity of a target product is 99.9% or more. Compared with currently reported methods for synthesizing a target product, the use of the ketoreductase and the mutant thereof for target product synthesis avoids the use of a metal catalyst, thereby making the process environmentally friendly and reducing the cost. In addition, the ketoreductase mutant simplifies the resolution process steps while improving the chiral purity of a target product, resulting in a simple synthesis method.

The invention provides a protein glutaminase mutant with synchronously improved enzyme activity and thermal stability and application of the protein glutaminase mutant, and belongs to the field of gene engineering and enzyme engineering. According to the invention, a series of mutants MT1-MT6 are obtained by carrying out molecular modification on a coding gene of protein glutaminase PG3 from Chryseobacterium sp. Compared with a wild type, the mutant MT6 has the advantages that the optimal catalytic temperature of the mutant MT6 is increased by 5 DEG C, the specific activity and the specific activity t65 DEG C 1 / 2 are 106.1 U / mg and 76.7 min respectively and are 2.69 times and 2.99 times of the specific activity t65 DEG C 1 / 2 of the wild type, the purpose of simultaneously improving the catalytic activity and the thermal stability of the protein glutaminase PG3 is achieved, and the application potential of the protein glutaminase in the industrial field is expanded. In addition, the mutant can significantly improve the foamability and stability of the myofibrillar protein of the spotted silver carp, and provides a potential enzyme resource for enhancing the functional characteristics of the protein through enzymatic deamidation modification.

The application discloses a method for preparing ganoderma polysaccharide rich in mannan-galactan structure by using a bacterium of the genus chryseobacterium GS-7 and ganoderma lucidum YJ0724 and application thereof, and relates to the technical field of microbial fermentation, and comprises the following steps: preparing a seed solution of the bacterium of the genus chryseobacterium GS-7 and a seed solution of the ganoderma lucidum YJ0724; a. inoculating the seed solution of the bacterium of the genus chryseobacterium GS-7 and the seed solution of the ganoderma lucidum YJ0724 into a fermentation medium at the same time for co-culturing to obtain a fermentation liquor containing ganoderma polysaccharide rich in mannan-galactan structure. The obtained ganoderma polysaccharide rich in mannan-galactan structure can improve immunoregulatory activity.

The present application relates to the field of microorganisms, in particular to an antibiotic-sensitive electrogenic bacterial strain Chryseobacterium T1 and its applications. The Latin name of the electrogenic bacterial strain is Chryseobacterium sp. , the strain name is Chryseobacterium T1, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the accession number CGMCC No: 34175, and the preservation time is April 14, 2025. The strain can efficiently couple extracellular electron transfer and exhibit excellent bioelectrocatalytic performance in BES. The strain shows significant advantages in current output, electron transfer efficiency and long-term stability. Its unique characteristics provide new possibilities for the application of BES technology in environmental remediation, sustainable energy development, wastewater treatment and bioelectronic devices.

The application relates to a Chryseobacterium sp. with a preservation number of CGMCC No. 25365 and a strain number of CP3; the Chryseobacterium sp. has the characteristics of promoting growth and degrading chlorpyrifos, can be prepared into a bacterial agent, can enhance the degradation of residual chlorpyrifos in rice, can promote the growth of rice, and is applied to the repair of chlorpyrifos residual pollution in a rice planting system; the Chryseobacterium sp. belongs to plant endophytic bacteria, can long-term colonize in rice, can reduce pesticide residues in crops on the premise of not affecting the quality of crops, can guarantee the quality and safety of agricultural products, and can realize green and safe production.

The present disclosure discloses a composition for skin anti-aging, skin lightening, or skin texture improvement containing a metalloproteinase derived from a strain of the genus Chryseobacterium as an active ingredient, and / or a composition for inhibition of lipofuscin accumulation or for removal of lipofuscin containing the metalloproteinase as an active ingredient. The composition provides an efficacy of skin anti-aging, skin lightening, or skin texture improvement by inhibition of lipofuscin accumulation or by removal of lipofuscin, and provides an effect of preventing, improving, or treating a disease caused by lipofuscin accumulation.

Disclosed in the present disclosure is a composition for skin aging resistance, skin whitening, or skin quality improvement containing, as an active ingredient, a metalloprotease derived from a strain of the genus Chryseobacterium, and / or a composition for inhibiting lipofuscin deposition or eliminating lipofuscin containing, as an active ingredient, the metalloprotease. The composition provides skin anti-aging, skin whitening or skin quality improving efficacy by inhibiting lipofuscin deposition or removing lipofuscin, and provides an effect of preventing, improving or treating diseases caused by lipofuscin deposition.

The application discloses a chryseobacterium S1-14 and application thereof in enzymolysis of alpha-1,3-glycosidic bond high-molecular polysaccharide, and belongs to the technical field of food, cosmetics and medicine. Chryseobacterium The chryseobacterium S1-14 is classified as chryseobacterium sp., is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No. 37778, a preservation date of February 10, 2026, and a preservation address of No. 3, Xili, Beichen West Road, Chaoyang District, Beijing City. The chryseobacterium S1-14 can be used for degrading macromolecular polysaccharides containing alpha-1,3-glycosidic bonds, has high degradation rate, mild reaction condition, and the advantage that the obtained product has low molecular weight, and can be applied to industrial production.

The purpose of the present invention is to provide a protein-containing composition in which the free amount of essential amino acids is increased. By using a protein deamidase and a protease derived from the genus Chryseobacterium, it is possible to improve the free amount of essential amino acids, foamability, foam stability, and / or emulsibility of a protein-containing composition.