31 results about "Chryseobacterium sp." patented technology

The invention relates to a preparation raw material and a preparation method of rubusoside, which belongs to the field of food additives. Stevioside is transformed into rubusoside by the catalysis of chryseobacterium JH (Chryseobacterium sp.JH) CCTCC NO:M209202 or beta-glucosidase produced by the same. Concretely, the stevioside is prepared into bacterium culture liquid or enzyme transformation liquid and is transformed into rubusoside under the conditions of proper liquid loading quantity, proper transformation temperature, proper pH, proper time and the like after a right amount of bacteria or enzymes are added, and the rubusoside with high purity is obtained after the transformed rough transformation liquid is further separated and purified. The invention provides a novel raw material for preparing the rubusoside, which expands the source of raw materials and has stronger practical value.

The invention provides a microorganism for promoting black nightshade to remove trace cadmium pollution in water and a method for removing cadmium pollution, aiming to purify drinking water subjected to cadmium pollution by virtue of the high-efficiency absorption characteristic of super-accumulated plants, namely black nightshade, to cadmium. In the method, the black nightshade is cultured in an aquaculture manner, and Chryseobacterium sp. LKS03 is inoculated to the black nightshade to promote the roots of the black nightshade to grow; and during purification of drinking water, the roots of black nightshade plants are immersed into the drinking water subjected to cadmium pollution, and each black nightshade plant correspondingly purifies about 1-1.2L of the drinking water in a purification cycle, wherein the purification time is for 2-12h according to the cadmium ion concentration (not higher than 200mug/L) in a water sample, and the capability of the black nightshade plants for purifying the water sample is optimum in the 45th-80th day in the culture cycle of the black nightshade. The method is specific to the cadmium pollution in the drinking water and has the advantages of good removal effect, simplicity and convenience for operation, wide applicable range, and great application potential in the aspect of drinking water purification.

The invention discloses Chryseobacterium sp. for removing phosphorus in sewage at low temperature and a separation culture method. The Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has the ability of removing the phosphorus in the sewage at low temperature under aerobic conditions. The experimental result shows that: in spite of obvious inhibition of a low-temperature environment, the Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has a remarkable effect of strengthening the removal of the phosphorus at low temperature, especially at the temperature of between 8 and 12 DEG C, and the removal rate of the phosphorus is improved by 10 percent; and the effluent standard is superior to the first-grade A effluent standard (water temperature is less than or equal to 12 DEG C, and the control index of the first-grade A standard is 0.5mg/L).

The invention provides a biological iron-removing manganese-removing functional bacterium, which relates to a microorganism. The invention solves the problems that the present biological oxidation manganese removing engineering flora is established by natural selection, the biochemical capability is weak, the manganese removing effect is poor; the establishment of the water treatment removing manganese biologic filter pond needs a long time for cultivation, domestication and startup, and the restoring period after the engineering flora is damaged is long. The biological iron-removing manganese-removing functional bacterium MSB-4(Chryseobacterium sp.) belongs to flavobacterium, is preserved in the General Microorganism Center of China Commission of Culture Collection Management, the storage date is 30 Octomber 2007, the storage number is CGMCC No.2239; strain MSB-4 is aerobic strain, the length is 1.5-2 micrometers, the width is 0.8-1.1 micrometers, the strain is short rod-shaped cell with two blunt ends; brown yellow colony is formed on the substrate, the colony is non-transparent and round; the strain MSB-4 has metachromatic granules and lipoid granules, has no brood cell; N, N, N<1>, N<1>-tetramethyl-para-phenylene diamine stain is positive. The biological iron-removing manganese-removing functional bacterium MSB-4 of the invention has the capability for removing the Mn<2+>, <Fe2+> in the water, Mn<2+> removing rate can reach up to 94.44 percent, and the Fe<2+> removing rate can reach up to 90.13 percent.

The invention provides application of carbonyl reductase ChKRED20 serving as a biocatalyst in asymmetric catalysis of multiple prochiral carbonyl substrates to generate corresponding chiral alcohols. The carbonyl reductase ChKRED20 comes from Chryseobacterium sp. CA49 and has high activity and enantioselectivity.

The invention discloses a strain of chryseobacterium and application thereof. The strain is the chryseobacterium; the strain is named as Chryseobacterium sp GZU-Ch01, and is preserved in typical culture Storage Center in China on December 19, 2017, and the deposit number is CCTCCNO: M2017810. The Chryseobacterium sp. GZU-Ch01 can effectively decompose insoluble potassium, phosphorus, silicon, calcium, sulfur and other components in coal gangue into quick-acting potassium, effective phosphorus, effective silicon, exchangeable calcium, effective sulfur and the like absorbed by plants as nutrients. In particular, the GZU-Ch01 can effectively dissociate the insoluble potassium from the coal gangue into the quick-acting potassium, provide a valuable source of potassium resources, has a good dissociation effect on other potassium-containing minerals, add other potassium-containing minerals such as potassium feldspar to the coal gangue, and can further enhance the potassium content of a mineral microbial compound fertilizer using the coal gangue as a base material.

The invention discloses a preparation method of a microbial flocculating agent for treating lead-zinc waste water. The preparation method comprises the steps of (1) separating out a strain Chryseobacterium sp with flocculating ability from soil polluted by lead and zinc; (2) inoculating a single strain in a seed medium to obtain a seed culture solution; (3) inoculating the seed culture solution obtained from step (2) in a fermenting medium, and culturing to obtain a fermentation culture solution; and (4) centrifuging the fermentation culture solution obtained from step (3) to obtain supernateor a supernate extractor which is the microbial flocculating agent. The preparation method is simple in process and low in cost, and the prepared flocculating agent has good effect of treating the lead-zinc waste water, and does not cause secondary pollution.

The invention discloses an algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control. Highly algicidal Chryseobacterium sp. GLY-4205 is separated from water in Taihu Lake, and has a preservation number of CGMCC No.8283, and an identification result of effective algicidal components separated and purified from a fermentation product of the highly algicidal Chryseobacterium sp. GLY-4205 show that the effective algicidal components comprise 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, 7-hydroxy-3-isobutyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione and hexahydropyrrolo[1,2-a]pyrazine-1,4-dione. The half lethal doses LD50 of the above four algicidal substances on Microcystis aeruginosa 9110 are 3.9[mu]g/mL, 0.95[mu]g/mL, 2.82[mu]g/mL, and 5.7[mu]g/mL respectively. Hexahydropyrrolo[1,2-a]pyrazine-1,4-dione has no lethal effect on synechococcus BN60, and the half lethal doses LD50 of the residual three algicidal substances on the half lethal doses are 17.19[mu]g/mL, 1.31[mu]g/mL and 3.84[mu]g/mL respectively. The algicidal Chryseobacterium sp. can be used for developing and producing novel biological algicides in order to be finally applied in the cyanobacterial bloom control of lakes.

The invention relates to a strain for efficiently converting stevioside into rubusoside and a culture method and application thereof. A strain of (Chryseobacterium sp.) 1433 is preserved in the ChinaGeneral Microbiological Culture Collection Center on July 6, 2020, the preservation number is CGMCC No.20188 and the address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Yard, West Beichen road, Chaoyang District, Beijing. The invention also relates to the culture method of the strain and the application of the strain in preparing the rubusoside. The (Chryseobacterium sp.1433) screened and separated by the invention can hydrolyze sophorose of the stevioside into glucosyl to generate the rubusoside and cannot further hydrolyze the rubusoside, so that the conversion rate of the biological strain to the stevioside reaches 100 percent, the yield of the rubusoside reaches 100 percent and the strain is completely superior to the reported related strains.

The invention provides a strain of Chryseobacterium for efficient degradation of picolinic acid, and applications thereof, and belongs to the technical field of biological engineering. According to the present invention, a strain of picolinic acid degradation bacterial Chryseobacterium is separated and screened, wherein the strain is classified and named Chryseobacterium sp, is preserved in the China General Microbiological Culture Collection Center, and has the preservation number of CGMCC NO.10699; and the Chryseobacterium sp can effectively degrade picolinic acid in a wide pH value range or wide temperature range or in a wide pH value range and wide temperature range, can be adopted as a biological strengthening preparation so as to be used in aerobic biological treatment of picolinic acid wastewater within a weak alkali to weak acidity range and a wide temperature range, and has broad application prospects.

The invention provides a Chryseobacterium sp. L31 strain of which the preservation number is CGMCC No. 4137, which is separated and obtained from a sewage treatment aeration tank of Shandong Huayang pesticide factory by an acclimating method. The strain can degrade cypermethrin pesticide of 50mg/L by about 71% in 3 days, and can maintain stable degradation ability. In addition, the cell surface of the strain has very high hydrophobicity, and the hydrophobicity of the cell surface can be adjusted for finishing adsorption degradation of the hydrophobic pesticide.

The invention relates to the field of microorganisms, and discloses Chryseobacterium sp., a fungicide containing the Chryseobacterium sp., application of the Chryseobacterium sp. And a method for degrading plastics. The strain name of the Chryseobacterium sp. PET-29 is Chryseobacterium sp. PET-29, and the preservation number of the Chryseobacterium sp. PET-29 is CCTCC (China Center for Type Culture Collection) NO: M 2022311. The bacterial agent provided by the invention contains the Chryseobacterium sp. The invention also provides the application of the Chryseobacterium sp. And the microbial inoculum in plastic degradation. The method for degrading the plastic comprises the step of contacting the Chryseobacterium sp. And/or the fungicide with the plastic. The Chryseobacterium sp. Strain or the bacterial agent containing the strain can be used for biodegrading polyethylene glycol terephthalate plastics, and has further research and development and engineering application prospects in the fields of novel plastic degrading enzyme mining and modification, waste plastic recycling, environmental plastic pollution bioremediation and the like.

The invention discloses chryseobacterium sp. JT03 capable of using methane. The preservation number of a strain is CCTCC No.M2010100. The chryseobacterium sp. can be used for preparing a methane biological absorbent, a garbage treating agent and a biological catalyst. The chryseobacterium sp. overcomes the application defects of low bacteria density of methane-oxidizing bacteria, difficulty in amplification culture of bacteria and the like, and has high affinity to methane. The chryseobacterium sp. is particularly suitable for methane artificial sources such as household garbage landfill sites, paddy fields and the like, and can be widely popularized.

The invention discloses a new strain of a producing strain XJ2 of a natural yellow pigment and preparation of pigment. It is indicated that the new strain is a typical new strain by identifying Chryseobacterium sp.XJ2 CGMCC No.13157, the natural yellow pigment is produced through activation, proliferation and extraction, the produced natural yellow pigment can be dissolved in water, methanol and ethanol and is easily dissolved in acetone, ethyl acetate, n-butanol and other ultralow-polarity solvents, the extract ethanol solution has a maximum absorption peak at the position of 438 nm, and the new strain has an anti-oxidation function, is stable in the pH range of 2.0-9.0, has good thermal stability and certain light stability, and has great practical significance for the production of the natural yellow pigment.

The invention discloses a method for preparing a bio-organic fertilizer from kitchen garbage. The method comprises the following steps: adding feathers into kitchen garbage, degrading the feathers into amino acids by using a bacterial agent prepared from a feather degrading bacterium, namely Chryseobacterium sp., and supplementing nutrients in the composting process of the kitchen garbage by using the amino acids, so that the activity of microorganisms is improved and humification degree of the kitchen garbage is accelerated. Besides, keratin waste and kitchen garbage waste are organically combined, a large amount of amino acids in feather is obtained through efficient feather degrading bacteria, the effect of replacing rapeseed meal compost in the prior art can be achieved, the cost is greatly reduced, and the final bio-organic fertilizer is high in activity, can effectively improve crop growth, and especially has a salt stress relieving effect on salinized soil in facility agriculture.

The invention belongs to the technical field of wine brewing microorganisms, and particularly relates to a novel Chryseobacterium strain and application thereof. The invention provides a novel strain of Chryseobacterium aiming at the problem that the variety of functional microorganisms capable of improving aroma in the fermentation process of Baijiu in the existing report is less. The preservation number of the novel strain of the Chryseobacterium is CGMCC (China General Microbiological Culture Collection Center) The new strain provided by the invention can simultaneously produce 2, 5-dimethylpyrazine, 2, 3-butanedione, 4-octanone, ethyl phenylacetate, indole, phenylacetaldehyde, dibutyl adipate and the like, provides a new strain for aroma production in white spirit brewing, enriches the resources of brewing microorganisms, and has a very good application value.

The invention relates to a novel efficient beta-glucosidase CsBGL as well as an encoding gene and application thereof. The amino acid sequence of the beta-glucosidase CsBGL is shown as SEQ ID NO. 2. The nucleotide sequence of the encoding gene of the beta-glucosidase CsBGL is shown as SEQ ID NO. 1. The beta-glucosidase CsBGL is derived from Chryseobacterium sp. 1433, the Chryseobacterium sp. 1433was preserved in the China General Microbiological Culture Collection Center on July 6, 2020, the preservation number is CGMCC No. 20188, and the address is Institute of Microbiology, Chinese Academyof Sciences, No. 3, No. 1 Yard, Beichenxi Road, Chaoyang District, Beijing City. The novel efficient beta-glucosidase CsBGL provided by the invention can hydrolyze sophorose of stevioside into glucosyl to generate rubusoside, the rubusoside cannot be further hydrolyzed, so that the conversion rate of the rubusoside reaches 100%, and the yield of the rubusoside reaches 100%.