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Chryseobacterium sp. capable of using methane and application thereof

A Chryseobacterium, methane technology, applied in the field of environmental biology, to achieve high affinity, easy to expand culture, and shorten the delay time

Inactive Publication Date: 2012-08-01
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The present invention aims at the deficiencies in the prior art that methane biomethane cannot be engineered, and provides a methane-utilizing Chryseobacterium JT03 and its application. The Chryseobacterium JT03 can grow rapidly by using economical carbon sources such as starch. On this basis It provides a very important biological basis for the engineering of methane biooxidation to enhance landfill methane emission reduction.

Method used

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  • Chryseobacterium sp. capable of using methane and application thereof
  • Chryseobacterium sp. capable of using methane and application thereof
  • Chryseobacterium sp. capable of using methane and application thereof

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1. Purification and identification of Chryseobacterium sp.JT03

[0039] The strain is Gram-negative, the colony is round or flat, yellow, white or orange, with smooth edges, transparent and shiny, and the diameter of the colony is about 1mm; the bacteria are rod-shaped or oval, immobile, aerobic, chemical Heterotrophic bacteria. The cells are about 0.4-0.6 μm wide and 1.0-1.3 μm long. SEM photo see figure 1 .

[0040] PCR products of strain JT03 were subjected to 3% agarose gel electrophoresis ( figure 2 ), it can be seen that the gel bands of PCR products are very clear. The DNA sequencing of the strain was entrusted to Dalian Bao Biological Company. The 16S rDNA base length of Chryseobacterium JT03 was determined to be 664bp. The base sequence was compared with the 3 strains of Chryseobacterium sp. in the GenBank nucleic acid sequence database. The homology is above 99%.

[0041] Table 1 Physicochemical properties of Chryseobacterium sp.JT03

[0042...

Embodiment 2

[0044] Embodiment 2. Growth curve and physicochemical properties of bacterial strain Chryseobacterium sp.JT03

[0045] Depend on image 3 It can be seen that the growth delay period of Chryseobacterium JT03 is 84h. Compared with the reported methane oxidizing bacteria, the delay time is shortened by about 24h. The logarithmic phase (84-156h) of the bacterial liquid is obviously turbid, and the OD value has increased from 0.05 to about 0.65. The bacterial concentration is higher than that of most methanotrophs reported.

Embodiment 3

[0046] Embodiment 3. Bacterial strain Chryseobacterium sp.JT03 utilizes multi-carbon compound proliferation experiment

[0047] Use NMS medium to prepare 7 kinds of sugar solutions such as 3g / L glucose, fructose, mannose, lactose, sucrose, starch, and raffinose for use; take 20mL of the above 7 kinds of NMS medium and add it to a 100mL serum bottle. Take another serum bottle and add 20mL NMS medium without adding carbon source as a control. Sterilize the above-mentioned medium by high-temperature steam at 121°C. After cooling, add 1mL of seed bacteria liquid into the bottle with a micropipette, cover the rubber stopper and shake well, put it in a shaker and set it at 30°C and 160rpm. Under culture 4d. Then measure the OD of the bacterial solution 560nm To determine the growth of the bacteria, about 1.2 mL of the bacterial liquid was extracted for high-speed centrifugation, and then the supernatant was used to detect the sugar concentration by the sulfate anthrone method to d...

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Abstract

The invention discloses chryseobacterium sp. JT03 capable of using methane. The preservation number of a strain is CCTCC No.M2010100. The chryseobacterium sp. can be used for preparing a methane biological absorbent, a garbage treating agent and a biological catalyst. The chryseobacterium sp. overcomes the application defects of low bacteria density of methane-oxidizing bacteria, difficulty in amplification culture of bacteria and the like, and has high affinity to methane. The chryseobacterium sp. is particularly suitable for methane artificial sources such as household garbage landfill sites, paddy fields and the like, and can be widely popularized.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a Chryseobacterium that can utilize methane and its application. Background technique [0002] Methanotrophs are a special kind of methylotrophic microorganisms that use methane as the only carbon source and energy source. They have a wide distribution range. Methanotrophs are found in many environments such as acid, alkali, salt, high temperature, low temperature, and barren. exist. Its special metabolic process and metabolites have a significant impact on the global environment and have attracted widespread attention. Methanotrophs not only play an important role in global methane consumption, but also in the carbon, nitrogen, and oxygen cycles. [0003] Methanotrophs are Gram-negative bacteria that are strictly oligotrophic. Since methanotrophs were first isolated in 1906, eight genera of methanotrophs have been discovered so far, mainly including ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01
CPCY02E50/343Y02A50/20Y02E50/30Y02P20/59
Inventor 赵天涛张丽杰赵由才全学军邓郁平
Owner CHONGQING UNIV OF TECH
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