2 results about "Methane-Oxidizing Bacteria" patented technology

Microbial origin protein (MOP) is regarded as an alternative to feed and food grade protein, offering a sustainable solution (negligible land footprint and a water footprint 14 times lower than that of soybean) to partially support the increased supply needs. Our goal is to incubate methane-oxidizing bacteria (MOB) by using bubble column bioreactor (BCB) with internal gas recycling. The production process of microbial origin protein consists of 4 steps: (a) fermentation step using a bubble column bioreactor (BCB) with internal gas recycling (polypropylene with a thickness of 11 mm) and working volume of 20 L and column dimension of 11.8 cm as internal diameter (D) and 213.0 cm as height (H), and a H / D ratio of 18.05; (b) biomass harvesting and concentration using centrifugation during 15 min at 3500 rpm and 4°C; (c) High Pressure Homogenization (HPH) processing was used to homogenize the biomass in four passes at pressure levels of 150 MPa with a duration of 4 min between the different passes; and (d) Spray-drying of the biomass using a spray dryer equipped with a rotary atomizer and the atomization pressure was maintained at 0.6 MPa. The methanotrophic biomass obtained exhibited an outstanding nutritional value. More specifically, a high protein content (~62%) along with a balanced amino acids profile, covering all dispensable and non-dispensable AAs, was produced by this process. Moreover, the lipid content (~8%) was also comparable to other nutrient sources such as fishmeal (8%). All the results obtained, and all the parameters determined in the present study are sufficient to implement a bubble column bioreactor at industrial scale and to produce the microbial origin protein (MOP) for animal and human nutrition.

This invention discloses a method for protein extraction and mass spectrometry pretreatment from methanogenic bacteria, belonging to the field of protein extraction technology. The method involves pretreating a methanogenic bacteria enrichment culture to remove inorganic salt ions and other impurities, resulting in a pretreated culture. Then, a lysis buffer composed of sodium dodecyl sulfate, Tris-HCl buffer, and a protease inhibitor is added to the pretreated culture, followed by ultrasonic disruption to obtain a cell lysate, from which a crude protein extract solution is obtained. Finally, the protein extract is extracted from the crude protein extract solution. This invention significantly improves protein extraction efficiency and peptide purity by optimizing the extraction process and subsequent enzymatic digestion, while preserving the methanogenic activity of the extracted protein as much as possible, providing more accurate and reliable sample support for downstream protein mass spectrometry analysis.