11 results about "Methane-Oxidizing Bacteria" patented technology

The present invention relates to novel enzyme nanoparticles capable of converting methane into methanol, in which key active sites of methane-oxidizing bacteria are fused with each other and expressed on a protein that can be self-assembled in cells to form nanoparticles, and specifically to enzyme nanoparticles including a protein having methane monooxygenase (MMO) activity and active sites of the methane oxidase, a method for production thereof, a recombinant microorganism into which a nucleic acid encoding the protein and the active site of the methane oxidase is introduced, and immobilized enzyme nanoparticles including the enzyme nanoparticles loaded on a carrier.

Microbial origin protein (MOP) is regarded as an alternative to feed and food grade protein, offering a sustainable solution (negligible land footprint and a water footprint 14 times lower than that of soybean) to partially support the increased supply needs. Our goal is to incubate methane-oxidizing bacteria (MOB) by using bubble column bioreactor (BCB) with internal gas recycling. The production process of microbial origin protein consists of 4 steps: (a) fermentation step using a bubble column bioreactor (BCB) with internal gas recycling (polypropylene with a thickness of 11 mm) and working volume of 20 L and column dimension of 11.8 cm as internal diameter (D) and 213.0 cm as height (H), and a H / D ratio of 18.05; (b) biomass harvesting and concentration using centrifugation during 15 min at 3500 rpm and 4°C; (c) High Pressure Homogenization (HPH) processing was used to homogenize the biomass in four passes at pressure levels of 150 MPa with a duration of 4 min between the different passes; and (d) Spray-drying of the biomass using a spray dryer equipped with a rotary atomizer and the atomization pressure was maintained at 0.6 MPa. The methanotrophic biomass obtained exhibited an outstanding nutritional value. More specifically, a high protein content (~62%) along with a balanced amino acids profile, covering all dispensable and non-dispensable AAs, was produced by this process. Moreover, the lipid content (~8%) was also comparable to other nutrient sources such as fishmeal (8%). All the results obtained, and all the parameters determined in the present study are sufficient to implement a bubble column bioreactor at industrial scale and to produce the microbial origin protein (MOP) for animal and human nutrition.

The present invention relates to the field of industrial microbial technology, and specifically discloses a kind of Acinetobacter pittii, its microbial agent and application. The Acinetobacter pittii (Acinetobacter pittii) JW535 has a deposit number of CGMCC No.25479. The bacteria capable of oxidizing methane provided by the present invention belong to Acinetobacter pittii, which breaks through the category that methane oxidizing bacteria are mainly concentrated in the genus Methylobacterium in the past. Acinetobacter pittii JW535 has a fast growth rate and a wide pH tolerance. It can efficiently carry out the methane oxidation process under the condition of environmental pH change, and can also efficiently oxidize low-concentration methane gas. At the same time, the strain has high salt tolerance and strong adaptability to the environment, can effectively reduce the greenhouse effect caused by methane emissions, and can also be used for coal mine gas elimination, playing a dual role of coal mine disaster reduction and atmospheric environmental protection, and has extremely high practical value.

This invention discloses a method for protein extraction and mass spectrometry pretreatment from methanogenic bacteria, belonging to the field of protein extraction technology. The method involves pretreating a methanogenic bacteria enrichment culture to remove inorganic salt ions and other impurities, resulting in a pretreated culture. Then, a lysis buffer composed of sodium dodecyl sulfate, Tris-HCl buffer, and a protease inhibitor is added to the pretreated culture, followed by ultrasonic disruption to obtain a cell lysate, from which a crude protein extract solution is obtained. Finally, the protein extract is extracted from the crude protein extract solution. This invention significantly improves protein extraction efficiency and peptide purity by optimizing the extraction process and subsequent enzymatic digestion, while preserving the methanogenic activity of the extracted protein as much as possible, providing more accurate and reliable sample support for downstream protein mass spectrometry analysis.

The invention belongs to the technical field of fire prevention and extinguishing of coal mine wells, and particularly relates to microbial gel foam for suppressing explosion and inhibiting spontaneous combustion of coal and a preparation method of the microbial gel foam. The microbial agent is prepared from the following components in parts by mass: 1-2 parts of a gelling agent, 0.1-0.6 part of a cross-linking agent, 0.2-0.4 part of a surfactant, 1-3 parts of a microbial liquid and 94-97.9 parts of water, the microbial liquid is obtained by mixing a methane-oxidizing bacteria liquid and a CO-oxidizing bacteria liquid according to the volume ratio of 1: (0.5-2). The microbial gel foam provided by the invention not only has the advantages of strong flow diffusivity, oxygen isolation, cooling, excellent inhibition performance and the like of the gel foam, but also provides a favorable environment for growth and reproduction of microorganisms due to the high water locking property and the network structure of the microbial gel foam, and prolongs the survival activity of the microorganisms in a mine goaf; goaf combustible gas can be metabolized through microorganisms, CO2 is generated, the goaf environment is inerted, and comprehensive prevention and control of goaf coal spontaneous combustion and releasing or reducing of the combustible gas are achieved.

The present invention provides a method and application for synchronous carbon sequestration and denitrification, belonging to the technical field of solid waste resource utilization. The present invention provides a method for synchronous carbon sequestration and denitrification, comprising: enriching the microbial population mainly composed of hydrogen-oxidizing bacteria in soil; enriching the microbial population mainly composed of sulfur-oxidizing bacteria in soil; enriching the microbial population mainly composed of methanotrophs in soil; after mixing and inoculating the methanotroph-enriched flora, sulfur-oxidizing bacteria-enriched flora and hydrogen-oxidizing bacteria-enriched flora into a domestication medium for domestication culture, inoculating them into a domestication medium containing biogas slurry for synchronous carbon sequestration and denitrification. The method of the present invention enriches and screens a composite microbial metabolic group in which multiple microbial populations mainly composed of methanotrophic bacteria coexist to form a synergistic metabolic system. The synergistic metabolic system has H2S tolerance and can metabolize methane, carbon dioxide in biogas and N, P nutrients in biogas slurry to achieve the purpose of synchronous carbon sequestration and denitrification.

The invention provides a synchronous carbon sequestration and denitrification method and application, and belongs to the technical field of solid waste resource utilization. The invention provides a synchronous carbon sequestration and denitrification method. The method comprises the following steps: enriching microbial populations mainly containing hydrogen oxidizing bacteria in soil; microbial populations mainly containing sulfur oxidizing bacteria in the soil are enriched; microbial populations mainly containing methane-oxidizing bacteria in the soil are enriched; the methane-oxidizing bacteria enriched flora, the sulfur-oxidizing bacteria enriched flora and the hydrogen-oxidizing bacteria enriched flora are mixed and inoculated into a domestication culture medium for domestication culture, and then the mixture is inoculated into a domestication culture medium containing biogas slurry for synchronous carbon sequestration and denitrification. According to the method disclosed by the invention, a synergistic metabolism system is formed by enriching and screening a compound microbial metabolism group in which a plurality of microbial populations coexist mainly including methane-oxidizing bacteria. The synergistic metabolism system has H2S tolerance, can metabolize methane and carbon dioxide in biogas and N and P nutrient substances in biogas slurry, and achieves the purpose of synchronous carbon sequestration and denitrification.

The invention belongs to the technical field of methane treatment, and particularly relates to a method for preparing a methane removal material and application of the method.The method comprises the steps that S1, methane-oxidizing bacteria in sludge are subjected to enrichment culture treatment, and inoculation liquid is obtained; s2, the inoculation liquid is subjected to fixation treatment, and a methane removal material is obtained; wherein the enrichment culture treatment comprises the following steps: (1) preparing a sludge suspension, and taking supernate to obtain an original inoculation solution; (2) carrying out passage treatment on the original inoculation liquid to obtain the inoculation liquid; the subculture treatment comprises the following steps: mixing an inorganic nutritive salt solution, methane and the original inoculation liquid, and carrying out subculture. The method for preparing the methane removal material has the advantages that the operation is simple, the condition is mild, the prepared material has a good methane removal effect, and the like, and the application prospect is wide. According to the methane removal material, methane in O2 in the environment is oxidized into CO2 and H2O under the condition of no human intervention by utilizing methane oxidizing bacteria in the methane removal material, so that the content of methane in the environment is reduced, and the material can realize in-situ efficient removal of methane in a drainage pipeline, can be stably used for a long time, and is environment-friendly and wide in application range.

The utility model provides an oxidation device for methane oxidizing bacteria. The oxidation device for methane-oxidizing bacteria comprises: a bottom plate; the constant-temperature water tank is fixedly mounted at the top of the bottom plate; the oxidation fermentation box is arranged on the constant-temperature water tank; the frame is fixedly mounted on the oxidation fermentation box; the lifting mechanism is arranged on the frame, and the lifting mechanism is used for driving the methane oxidizing bacteria containing mechanism to move up and down; and the methane oxidizing bacteria containing mechanism is arranged at the bottom of the lifting mechanism, and the methane oxidizing bacteria containing mechanism is used for containing a culture medium and methane oxidizing bacteria. The oxidation device for methane-oxidizing bacteria provided by the utility model has the advantages that the culture medium and the methane-oxidizing bacteria can be conveniently put in and taken out, the operation is convenient, and a constant-temperature fermentation function is realized.

The invention provides a method and a device for treating sewage by utilizing a bacterium-algae heterotrophic bacterium compound system, and belongs to the technical field of water treatment and recycling. Aiming at recycling of soluble methane and diversified removal of various pollutants such as carbon dioxide, organic carbon and nutritive salt, a specific microalgae-methane-oxidizing bacteria-heterotrophic bacteria compound microorganism system is constructed, efficient removal efficiency parameters are limited, ternary microorganism synergistic interaction is achieved, and the synergistic interaction target of reducing pollution and carbon is achieved. The MBR membrane assembly is adopted to intercept microbial effluent, and a specific efficient microbial community is maintained; the closed reactor aims at a specific removal object, dissolved gas is prevented from escaping, and recycling of soluble methane is guaranteed. The closed membrane bioreactor is suitable for culturing a specific ternary suspended microflora and can effectively treat soluble gas.

A device and method for bidirectional flow biological oxidation of ultra-low concentration coalbed methane, the method comprising a biological filter startup stage and a biological oxidation of ultra-low concentration coalbed methane stage. In the biological filter startup stage, a bacterial solution made from activated sludge is pumped into the gas phase reaction zone and all porous carriers are immersed, so that the microorganisms adhere to the surface and pores of the carriers. After a period of time, all liquids in the device are drained and recovered, leaving only a layer of liquid film on the surface of the filler. Ultra-low concentration coalbed methane is alternately introduced into the gas phase reaction zone from both ends of the device to provide a carbon source for the aerobic methane oxidizing bacteria in the biofilm on the surface of the filler, enabling them to grow and reproduce rapidly, and this operating mode is maintained until the device is successfully started. In the biological oxidation of ultra-low concentration coalbed methane stage, the circulating bacterial solution used in the startup stage is replaced with a sterile fresh nutrient solution, and the remaining operations are the same. In this stage, the aerobic methane oxidizing bacteria in the biofilm selectively oxidize the methane in the ultra-low concentration coalbed methane into carbon dioxide and water.