Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria

Abstract

The invention discloses an oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria, which comprises the following steps: after homogenizing a soil sample, extracting sample DNA (deoxyribonucleic acid), and quantifying propane oxidizing bacteria and methane oxidizing bacteria by a fluorescence quantitative PCR (polymerase chain reaction) technique; determining anomaly thresholds of the propane oxidizing bacteria and methane oxidizing bacteria, establishing a microbiological anomaly reference system for verification zone oil-gas microbiological exploration, drawing isoline of the propane oxidizing bacteria, and carrying out microbiological anomaly zoning; and marking the gas-cap reservoir and pure reservoir in the anomaly zone according to the content of the methane oxidizing bacteria. According to the method, the anomaly of light hydrocarbon oxidizing bacteria is utilized to perform oil-gas exploration; in the precision detection aspect, the quantity of the light hydrocarbon oxidizing bacteria in the sample is directly detected to avoid the artificial interference in the traditional culture method; and the detection quantity is higher than that of the traditional culture method by 1-2 orders of magnitude, the time is shortened to 12 hours from 14-21 days in the traditional culture method, and the method can detect 96 samples at one time, thereby effectively enhancing the precision and detection efficiency of microbial oil-gas exploration.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an oil and gas microbial exploration method that adopts molecular biology technology and is applied to microbial oil and gas exploration to effectively avoid surface pollution or near-surface biogenesis. Background technique [0002] Oil and natural gas are the basic energy related to the national economy and people's livelihood. After years of 3D seismic exploration, most of the oil and gas reservoir resources that are easy to be found in my country have been proven and developed. Small-scale, unstructured and hidden oil and gas reservoir resources have become the primary targets of current exploration. However, the cost of seismic exploration for these oil and gas resources is too high and data analysis is difficult. [0003] Oil and gas microbial exploration technology is a branch of near-surface geochemical exploration technology, which has the characteristics of directness, ef...

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Problems solved by technology

However, since the same amount of biogenic methane can be detected at the same depth in non-oil and gas areas, the methanotrophs in the near-surface soil cannot specifically indicate the micro-migration of methane in the lower oil and gas reservoirs, making methanotrophs as oil and gas reservoir exploration Risk of False Positives

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