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Method for cultivating methane-oxidizing bacteria

A methane-oxidizing bacteria and culture method technology, applied in a fast, high-density culture field, can solve problems such as complex process, explosion, increased power consumption, etc., achieve broad application prospects, simple culture method, increase growth rate and cell effect of density

Inactive Publication Date: 2008-01-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when the mixing ratio of methane and air or oxygen exceeds a certain range, there will be a danger of explosion; increasing the airflow rate will inevitably cause a large amount of gas to circulate, resulting in complex processes and increased power consumption

Method used

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  • Method for cultivating methane-oxidizing bacteria
  • Method for cultivating methane-oxidizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the comparison of the methane-oxidizing bacteria culture method of the present invention and the methane-oxidizing bacteria conventional culture method

[0022] Take the methane-oxidizing bacterium Methylosinus.trichosporium R (CGMCC NO.1894), which was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on December 21, 2006, referred to as CGMCC, and the address is: North Zhongguancun, Haidian District, Beijing, China One No. 13, the preservation number is CGMCC NO.1894) as an example, compare the cultivation effect of the cultivation method of the present invention and the conventional cultivation method of methane oxidizing bacteria, and the specific process is as follows:

[0023] 1. Routine cultivation of methane-oxidizing bacteria Methylosinus.trichosporium R (CGMCC NO.1894)

[0024] The methane oxidizing bacteria Methylosinus.trichosporium R CGMCC NO.1894 is cultivated by a conventional method, an...

Embodiment 2

[0032] Embodiment 2, the cultivation of methanotrophs

[0033] Methane oxidizing bacteria Methylosinus trichosporium OB3b (ATCC 55314) is cultivated with the method of the present invention, and specific method is as follows:

[0034] 1) Methane solvent saturation treatment of n-hexadecane

[0035] Put 20 mL of n-hexadecane into a 100 mL airtight glass culture bottle with a baffle, and after autoclaving at 121°C for 15 minutes, inject 40 mL of methane into the bottle with a sterilized gas filter with a pore size of 0.2 μm. , shaking at 30° C. and 150 rpm for 12 hours (with methane replenished through a 0.2 μm gas filter every 3 hours), to obtain n-hexadecane saturated with methane.

[0036] 2) Using methane-saturated n-hexadecane to cultivate methanotrophs

[0037] In a 100mL airtight glass culture bottle with a baffle, 30mL of NMS liquid medium was subpackaged, then 3mL of n-hexadecane saturated with methane was added, autoclaved at 121°C for 15 minutes, cooled, and then us...

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Abstract

The invention discloses a method for culturing a methane-oxidizing bacterium, which adds cetane in the culture medium based on the conventional method of the methane-oxidizing bacterium. Cetane is sheared by the mechanical force so as to form the micro dispersion droplet in the process that methane-oxidizing bacterium is shake cultured, the droplets can improves the dissolvability of methane and the cell can shuttle between the water phase and the organic phase owing to the compatibility of the bacterial cell. The invention can improve the growth speed and the cell density of the methane-oxidizing bacterium, which has the simple culture method, and has the wide application prospect.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a rapid and high-density culturing method for methane-oxidizing bacteria. Background technique [0002] Methanotrophs are a special kind of microorganisms in nature, which use methane as the only carbon source and energy source. Although some methanotrophs can also utilize methanol simultaneously, all methanotrophs cannot grow on polycarbonate as a carbon source. [0003] Methane-oxidizing bacteria not only play an important role in global methane greenhouse gas control, but also play a non-negligible role in the element cycle of aquatic and terrestrial ecological environments. With its diverse catalytic functions, methanotrophs have great application potential in the production of bulk chemicals, the development of new functional enzymes, the safety warning and emergency technology development of gas, and the bioremediation of environmental pollutants. [0004] The fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/26B01D53/84
CPCY02A50/20Y02P20/59
Inventor 邢新会吴昊韩冰江皓缑仲轩
Owner TSINGHUA UNIV
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