Methane gas thin-layer medium electrochemical biosensor and preparing method thereof

A biosensor, methane gas technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high cost, complicated operation and zeroing process, poor quantitative accuracy of methane detector, etc., to achieve thin and uniform diameter , Improve the detection selectivity and sensitivity, and the effect of good conductivity

Active Publication Date: 2016-02-24
HENAN INST OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a methane gas thin-layer dielectric electrochemical biosensor and its preparation method, which can effectively solve the problem o

Method used

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  • Methane gas thin-layer medium electrochemical biosensor and preparing method thereof
  • Methane gas thin-layer medium electrochemical biosensor and preparing method thereof
  • Methane gas thin-layer medium electrochemical biosensor and preparing method thereof

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Embodiment 1

[0048] Methane gas thin layer dielectric electrochemical biosensor of the present invention, refer to figure 1 , including the counter electrode 1, one end of the counter electrode 1 is reserved for connection to the electrochemical workstation when detecting methane, the rest is wrapped with electrolytic paper 2, and the exterior of the electrolytic paper 2 is wrapped with carbon fiber monofilament 3, wherein the carbon fiber monofilament 3 One end is suspended in the air for connection to an electrochemical workstation when detecting methane, and methane-oxidizing bacteria 4 are attached to the carbon fiber monofilament 3 .

Embodiment 2

[0050] The preparation method of the methane gas thin-layer dielectric electrochemical biosensor in this embodiment includes the following steps:

[0051] (1) Pick the methanotrophic bacteria and inoculate them in the liquid culture medium, seal it, add methane gas according to 10% of the air volume, and feed the liquid culture medium with methane gas of the same volume as the liquid culture medium, at 28°C Cultivate for 23 days, then mix the bacterial culture solution and the liquid medium according to the volume ratio of 1:200, seal it, add methane gas according to 10% of the air volume, and pass into the liquid medium the same volume as the liquid medium Methane gas, cultivated at 28°C for 9 days;

[0052] (2) Soak a 10cm-long carbon fiber bundle in acetone for 15 minutes, remove the colloidal coating on the surface of the carbon fiber bundle, wash it with water, and dry it at 90°C; then place it in concentrated nitric acid for 10 minutes at room temperature and oxidize it,...

Embodiment 3

[0058] The preparation method of the methane gas thin-layer dielectric electrochemical biosensor in this embodiment includes the following steps:

[0059] (1) Pick methanotrophic bacteria strains and inoculate them in the liquid medium, seal it, add methane gas according to 10% of the volume of the air, and pass into the liquid medium the same volume of methane gas as the liquid medium, at 30°C Cultivate for 20 days, then mix the bacterial culture solution and the liquid medium according to the volume ratio of 1:200, seal it, add methane gas according to 10% of the air volume, and pass into the liquid medium the same volume as the liquid medium Methane gas, cultivated at 30°C for 7 days;

[0060] (2) Soak 10cm-long carbon fiber bundles in acetone for 5 minutes, remove the colloidal coating on the surface of the carbon fiber bundles, wash with water, and dry at 90°C; then oxidize in concentrated nitric acid for 20 minutes at room temperature, take it out, wash with water, and d...

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Abstract

The invention discloses a methane gas thin-layer medium electrochemical biosensor and a preparing method thereof. The biosensor comprises a counter electrode, a section at one end of the counter electrode is reserved for being connected with an electrochemical workstation when methane gas is detected, the remaining part of the counter electrode is wrapped with electrolytic paper, the outer portion of the electrolytic paper is wound by a carbon fiber monofilament, one end of the carbon fiber monofilament is suspended and used for being connected with the electrochemical workstation when methane gas is detected, and methane-oxidizing bacteria are attached to the carbon fiber monofilament. The concentration of methane gas is detected through an electrochemical method, the high-temperature condition of a heat conduction method is not needed, and safety is fully ensured under the normal-temperature solution condition. The methane-oxidizing bacteria serve as a biological catalyst, and the detection selectivity and sensitivity on methane gas are improved through the features of high specificity and high selectivity of the biological catalyzing process.

Description

technical field [0001] The invention relates to a methane gas thin-layer dielectric electrochemical biosensor and a preparation method thereof, belonging to the technical fields of coal mine gas concentration monitoring and natural gas leakage detection. Background technique [0002] The main power energy of my country's national economic construction is coal, which accounts for more than 70% of my country's total energy structure. At present, there are 26,000 coal mines recorded in my country, all of which are gas mines, and more than half of them are high-gas mines. Coal mining is subject to certain conditions, including natural geological conditions, underground mining conditions, miners' skills and equipment, etc. Therefore, coal mining has problems such as poor safety and reliability, and a high incidence of safety accidents, the most serious of which is gas accidents. The frequent occurrence of gas accidents has hindered the development of the coal industry, brought g...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/327G01N27/48
Inventor 谷保祥王喜英周继军刘碧波曹志林
Owner HENAN INST OF ENG
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