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Process for producing methyl hydride oxidized bacteria agent

A technology for methane-oxidizing bacteria and bacterial agent is applied in the field of mixed culture of methane-oxidizing bacteria. , Easy to store and transport, Simple preparation method

Inactive Publication Date: 2009-07-29
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acquisition of pure strains requires the screening of bacterial strains, which requires multiple subcultures, and the process of strain cultivation and pure strain isolation is very complicated.
Moreover, the preservation of pure strains is complicated. If it is necessary to add a protective agent to store at a temperature below zero, it is also necessary to avoid contamination by miscellaneous bacteria.

Method used

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  • Process for producing methyl hydride oxidized bacteria agent
  • Process for producing methyl hydride oxidized bacteria agent
  • Process for producing methyl hydride oxidized bacteria agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mineralized waste was collected from Shanghai Laogang Domestic Waste Landfill. One mineralized waste was extracted from different landfill units No. 1 to No. 5 as a sample, a total of 5 samples. After sieving the mineralized waste of 5 samples to remove impurities, the pH value was adjusted to 7, and the water content was about 25%. Then put into the serum bottle and pass CH 4 and air (the volume ratio of methane and oxygen is methane: oxygen = 1:1 ~ 1:1.5), at about 30°C, carry out the enrichment culture of methanotrophic bacteria until the methane oxidation rate in the serum bottle is detected to exceed 10%. The mineralized waste with good enrichment and cultivation effect of methanotrophic bacteria was obtained, as shown in Table 1.

[0021] Table 1 Enrichment and cultivation effects of mineralized waste in different landfill units

[0022] Landfill unit initial methane volume (mL) methane volume after enrichment (mL) methane oxidation rate (%)

[0023] Unit 1 97....

Embodiment 2

[0032] Take 0.5 g of mineralized waste from No. 1 landfill unit with the best enrichment effect in Example 1 and put it into a serum bottle, add 100 mL of improved NMS culture solution, mix, and shake on a shaker at 115-140 r / min to adjust the pH is 9, then pass into CH 4 and air (the volume ratio of methane and oxygen is methane: oxygen = 1: 1; the feed rate of the mixed gas is 130-150mL / d·100mL culture solution), and the temperature is 25-34°C for shaking bed cultivation. Regularly analyze the methane content in the serum bottle, analyze the methane oxidation amount and the change of the methane oxidation rate, if the methane oxidation rate exceeds 1.5mL / h, the methanotroph bacteria agent is prepared.

[0033] pass image 3 From the test results shown, it can be seen that the initiation of methanotroph culture is faster. Two days after the maximum methane oxidation rate appeared: the highest methane oxidation rate was 2.87mL / h, which appeared at 56h of culture.

Embodiment 3

[0035] The mineralized waste from No. 1 landfill unit with the best enrichment effect was put into a serum bottle, and the improved NMS culture solution was added. The ratio of mineralized waste to culture solution was 1g:100mL. The mixed solution was shaken on a shaker to adjust the pH value, and then passed through CH 4 and air (the volume ratio of methane and oxygen is methane: oxygen = 1: 1.2; the feed rate of the mixed gas is 170-180mL / d·100mL culture solution), the temperature is 25-34°C, and the shaker is under the condition of 115-140r / min. bed culture. Regularly analyze the methane content in the serum bottle, analyze the methane oxidation amount and the change of the methane oxidation rate, if the methane oxidation rate exceeds 1.5mL / h, the methanotroph bacteria agent is prepared.

[0036] pass Figure 4 From the test results shown, it can be seen that the initiation of methanotroph culture is faster. The maximum methane oxidation rate appeared for about two days:...

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Abstract

The invention discloses a preparation method of methane-oxidizing bacteria preparation and relates to a mixed culturing method of methane-oxidizing bacteria. Mineralized waste is employed as the carrier and the seed source of the methane-oxidizing bacteria. Enrichment of the methane-oxidizing bacteria is carried out on the mineralized waste; then the enriched mineralized waste is added into the culture fluid of the improved formula of NMS culture fluid used for culturing normal methane-oxidizing bacteria; then the mixed gas of methane and oxygen with a volume ratio of 1:1 to 1: 1.5 is pumped to carry out culture. When the method is employed, the effect for culturing the methane-oxidizing bacteria is good and the starting is so quick that the largest methane oxidation rate can appear in about 2 days. The mixedly cultured methane-oxidizing bacteria avoid the problems that the traditional strain culture method and axenic isolation process are complex and the pure strains are not easily preserved. The preparation of the invention has a simple preparation method, and a remarkable methane oxidizing effect; moreover, the bacteria liquid is easy to be stored and transported. The preparation can be conveniently used for controlling the methane discharge in a refuse landfill and can also provide the methane-oxidizing bacteria strain for industrial production.

Description

technical field [0001] The invention relates to a method for mixed cultivation of methane-oxidizing bacteria, in particular to cultivating methane-oxidizing bacteria by using mineralized garbage as a strain source to prepare a methane-oxidizing bacteria agent. Background technique [0002] Methane is a long-term atmospheric greenhouse gas that contributes 26 times more to the greenhouse effect than carbon dioxide. Domestic waste landfills are one of the main sources of methane, which account for about 1.5% to 15% of the total global methane emissions. Therefore, controlling and reducing methane emissions from landfills is of great significance to reducing global greenhouse gas emissions. Most landfills in my country do not have a landfill gas recovery system. The release of methane gas from these landfills brings safety hazards to the surrounding environment. Methane gas entering the atmosphere has a greenhouse effect and will exacerbate global warming), and methane emissio...

Claims

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Application Information

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IPC IPC(8): C12N1/00
Inventor 赵由才梅娟蒋家超黄仁华陈浩泉
Owner TONGJI UNIV
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