79results about How to "Effectively eliminate interference" patented technology

The invention provides a method for analyzing nicotine chiral isomers in tobacco and smoke. The method specifically comprises the following steps: (1) pretreating a sample: treating the tobacco sample, so as to obtain a solution to be detected; (2) preparing a standard solution; (3) carrying out qualitative detection on the sample: carrying out ultrahigh-performance bonded-phase chromatography detection on the solution to be detected and the standard solution separately, comparing retention time to carry out qualitative detection, and determining ingredients of the nicotine chiral isomers in the solution to be detected; (4) carrying out quantitative detection on the sample: carrying out ultrahigh-performance bonded-phase chromatography detection on the solution to be detected and the standard solution separately, and carrying out quantification by adopting an external standard method, so as to obtain the content of the nicotine chiral isomers in the solution to be detected. According to the method for analyzing the nicotine chiral isomers in the tobacco and smoke, provided by the invention, nicotine serves as an object of study, liquid, solid and gaseous tobacco samples are pretreated, and ultrahigh-performance bonded-phase chromatographic conditions are optimized, so that the sensitivity is high, the repeatability is good, and the accurate determination of composition of the nicotine chiral isomers can be achieved.

The invention discloses a representation method of oil-gas exploration and oil-gas reservoir by taking vital bacterium abnormality and dead bacterium abnormality of methane-oxidizing bacteria as indicators, comprising the following steps: collecting a sample above an oil-gas exploration and oil-gas reservoir representation area according to the specific grid degree, the sampling depth and the sampling amount, packaging and saving the sample according to the specific condition; adopting a conventional microorganism counting method to obtain the vital bacterium quantity and the dead bacterium quantity of the methane-oxidizing bacteria in the sample; drawing an equal bacterium line of the vital bacteria and an equal bacterium line of the dead bacteria on a map, a topographic map, a geologic map or a topography-geologic map to obtain the vital bacterium abnormality and the dead bacterium abnormality; comprehensively comparing the vital bacterium abnormality and the dead bacterium abnormality; and estimating and predicting the underground oil-gas resource condition of the oil-gas exploration and oil-gas reservoir representation area, or extracting an exploitation scheme of the oil-gas reservoir according to the characteristics of the vital bacterium abnormality and the dead bacterium abnormality and the differences of the vital bacterium abnormality and the dead bacterium abnormality, and combining the results of geological exploration, geochemical exploration and geophysical exploration.

The invention discloses a high performance liquid chromatography method for determination of benzalkonium chloride content in antiseptic wipes. The method comprises following steps: (1) weighting a sample of the antiseptic wipes; (2) taking acetonitrile-70 mmol/l ammonium acetate and glacial acetic acid as mobile phase; (3) adding the antiseptic wipe sample and 25 ml mobile phase into a separation column, extracting the sample with mobile phase for 15 min, performing vortex oscillation for 2 min and diluting the mobile phase, wherein a column temperature of the separation column is 40 DEG C, and a determining wavelength is 262 nm; and (4), after dilution, cooling and then transferring the sample into a 100 ml volumetric flask, diluting with mobile phase to 100 ml, and then analyzing. The benzalkonium chloride content is determined by an external standard method, and the benzalkonium chloride content of the antiseptic wipe sample is compared with that of a benzalkonium chloride standard solution.

The invention provides an unmanned secure communication authentication protocol and belongs to the field of unmanned motor vehicle ad hoc network information and communication. A vehicle obtains private data from a TA (Trusted Authorizer), generates a pseudonym and a corresponding private key offline and realize user authentication through bilinear mapping, so safety and privacy of the vehicle areensured. According to the protocol, hash chain elements are taken as keys for generating message authentication codes. In a delay exposure mode, a receiver receive the hash chain elements obtained ina delay mode and verify whether the last message authentication code can be generated or not. According to the protocol, a prediction value is generated and is sent to the receiver, so the receiver can verify validity of a message in real time, and robustness of the protocol is improved. According to the protocol, anonymity and privacy protection effects are achieved, and verifiability and integrity of data can be ensured. The protocol is also characterized by non-repudiation, non-relevance, conditional privacy protection, replay resistance and packet loss resistance. Normal communication authentication can be ensured.

The invention discloses a method for implementing oil-gas exploration and oil-gas reservoir characterization by using living bacteria exception and total bacteria exception of methylosinus trichosporium as indexes. The method comprises the following steps of: acquiring a sample above an oil-gas exploration and oil-gas reservoir characterization area according to the specific grid degree, samplingdepth and sampling quantity, and packing and storing the sample according to the specific conditions; simultaneously acquiring the living bacteria number and the total bacteria number of the methylosinus trichosporium in the sample by adopting a conventional microbe counting method, and drawing an equal living bacteria line and an equal total bacteria line on a map, a topographic map, a geologic map or a topographic-geologic map to obtain the living bacteria exception and the total bacteria exception; comprehensively comparing the living bacteria exception with the total bacteria exception; making a judgment and a prediction or providing an oil-gas reservoir exploitation scheme for the underground oil-gas resource condition of the oil-gas exploration and oil-gas reservoir characterizationarea according to the characteristics, similarities and differences of the living bacteria exception and the total bacteria exception by combining the results of geologic exploration, geochemical exploration and geophysical exploration.

The invention discloses a pendent drop liquid-liquid extraction method, which adds un-analyzed liquid sample in the extraction bottle with agitating, and quantitatively abstract extraction agent and drop it on the sample liquid. When the time is up to the setting time, use sampler to absorb the extraction agent, and use the analyzing instrument to detect. The method of the invention needs little amount of sample and can effectively exclude the interference of co-existing components of the compled samples to realize high multiplying factor gathering and to lower the analyzing limit and cost. It is very convenient for the invention to unite all kinds of chromatogram, spectrum and electro-chemical analyzing method. The invention can be utilized to food safety detection, environmental monitoring, natural resources extraction and the analyzing procedure of medicine and chemicals' manufacturing. The invention has strong technological priority and wide utilization foreground.

The invention provides a pretreatment method for detecting pesticide residues in fresh tea leaves. The method comprises the following steps: selecting a dispersing agent which has high adsorptive property on pesticides such as organic chloride and pyrethroid, directly mixing and grinding the dispersing agent with a fresh leaf sample, so that the cell disruption degree of the sample is improved, and the sample is finally uniformly dispersed; obtaining a mixture in a half-dry state and taking the mixture as a filler, feeding the filler in a chromatographic column in which a certain amount of adsorbents and water removal agents are fed in advance, eluting by using a mixed organic solvent, concentrating an eluant, so that the eluant can be directly subjected to instrumental analysis. According to the method, the operations of extraction and purification of the sample are integrated, and loss of the measured substance caused by sample homogenization, precipitation, centrifugation, dissolution, emulsification and concentration is avoided. Moreover, the method is simple and convenient to operate, high in recovery rate, small in solvent consumption and low in analysis cost and is a green and environment-friendly sample pretreatment method.

The invention relates to a high-amplitude shielding layer rejecting method based on seismic data instantaneous attributes. The method is characterized by comprising the following steps: 1), fine interpretation of a high-amplitude shielding layer is carried out in terms of data after stack or offset; 2), the interpreted high-amplitude shielding layer is regarded as a center; a time window is designed, and seismic data in the time window is intercepted; time and amplitude information of the interpreted high-amplitude shielding layer is obtained, and the seismic data in the time window is subjected to Hilbert conversion to obtain frequencies and phases forming wavelets of the high-amplitude shielding layer; average wavelets are obtained through well seismic calibration; according to correlation coefficients between the average wavelets and different scale parameter theoretical wavelets, the scale parameters of the average wavelets are determined; Morlet wavelets approximating the high-amplitude shielding layer is constructed one by one; and 4) differences between a data body of the 1) step and the Morlet wavelets obtained in the 3) step are calculated one by one to obtain seismic records in which the high-amplitude shielding layer is rejected. In the invention, influences by the high-amplitude shielding layer in a seismic profile on the processed data can be effectively eliminated.

The invention discloses a method for detecting chlorine dioxide content in life drinking water, which includes: measuring ClO2 homologous to four fifth of the total amount residual and the total by a first titration; and then measuring all the ClO2[-] in the water sample by a second titration. The method adopts chemical analysis to detect chlorine dioxide content in water, can effectively exclude other ion interference, avoids affection of accessory substances ClO2[-], ClO3[-] and the like ions, thereby accurately detecting the content of chlorine dioxide in the life drinking water; the minimum detection amount of ClO2[-] can achieve 0.02mg, the minimum detectable amount of ClO2 can achieve 0.08mg / L; moreover, the method has simple operation, and suitable promotion.

The invention discloses a traffic signal lamp state recognition method, device and equipment and a storage medium. The method comprises the following steps: determining at least one alternative traffic signal lamp detection result according to an image acquired by a vehicle; determining a target traffic signal lamp matched with the vehicle position information from the alternative traffic signal lamps according to map data; according to the relative position relationship between the target traffic signal lamp and the vehicle, mapping the target traffic signal lamp into the image to obtain a traffic signal lamp mapping result; and determining a traffic signal lamp state according to the traffic signal lamp mapping result and the alternative traffic signal lamp detection result. By using thetechnical scheme of the embodiment of the invention, the state of the traffic signal lamp in front of the vehicle can be accurately identified.

The invention discloses a method for determining content of a nerve growth factor in a nerve growth factor preparation. According to the method, the content of the nerve growth factor is determined by using gel chromatography in ultra-performance liquid chromatography (UPLC); gel chromatography conditions are presented as follows: a gel chromatography column is a UPLC molecular sieve chromatography column of which the molecular weight detection range is 1000 to 80000 Daltons and the pore diameter is 125 to 200 angstroms; the mobile phase is phosphate buffer of which the pH value is 6.5 to 7.2 and the concentration is 0.1 to 0.5M, and contains an organic phase of 5 to 20 percent by volume; the column temperature is 30 to 50 DEG C; the flow rate of the mobile phase is 0.1 to 0.5ml/min; a titanium flow cell is adopted by a detector, and the detection wavelength is 205 to 280nm. According to the method for determining the content of the nerve growth factor in the nerve growth factor preparation, disclosed by the invention, the NGF in the preparation can be accurately quantified.

The invention discloses a small dim target energy accumulation enhancement method capable of combining with a time-space domain. The method comprises the following steps that: firstly, adopting an anisotropy differential algorithm to carry out background suppression on an image to be processed to reduce the interference of background clutters; then, combining with the multi-frame movement characteristic of a small dim target in a neighborhood, adopting a target energy enhancement method of high order accumulation to obtain an energy maximum value in the neighborhood of continuous multi-frame images to realize the effective accumulation of movement target energy so as to achieve a purpose of enhancement; and finally, adopting a constant false alarm hypothesis test method to carry out segmentation extraction on the enhanced image to make the target prominent. Compared with a traditional singe-frame enhancement method, the method disclosed by the invention is characterized in that the dim target energy is accumulated in the movement neighborhood of the target according to the movement characteristics of the target, an enhancement effect better than the enhancement effect of the singe-frame enhancement method is realized, Gaussian noise in the image is effectively inhibited, and the signal-to-noise ratio of the image is obviously improved.

The invention discloses a content detection method for a compound houttuynia cordata mixture. The content detection method mainly includes that water-saturated normal butanol is used for extraction, water-alcohol elution is performed after column feeding, Phenyl bonded silica gel is taken as a chromatographic column, and mobile phase is acetonitrile-0.5% acetic acid solution (volume ratio is 18.5:81.5). By the content detection method, the problem that quercitrin which serves as a feature ingredient of the compound houttuynia cordata mixture cannot be controlled effectively is solved, content of quercitrin, baicalin and forsythin in the compound houttuynia cordata mixture is detected synchronously, and drug safety, effectiveness and quality controllability are guaranteed.

The present invention relates to a method for determining element sulfur content in distillate by adopting oscilloscopic polarography. Said method includes the following steps: adding triphenyl phosphorus in the sample to make it be reacted with element surfur to form triphenyl phosphonosulfide, preparing standard solution with different element sulfur contents, making the polarographic wave crest electric current value produced by triphenyl phosphonosulfide in standard solution be related to its element sulfur content to create standard curve, then utilizing polarographic wave crest electric current value produced by tested sample and making it pass through the standard curve so as to obtain the element sulfur content in the sample.

The invention discloses a high purity lipiarmycin A4 preparation method which comprises the following steps: A, filtering lipiarmycin A4 fermentation broth to obtain mycelium, soaking the mycelium with a polar solvent for solid-liquid separation to obtain lipiarmycin A4 extracting solution; B, concentrating the lipiarmycin A4 extracting solution, extracting the lipiarmycin A4 extracting solution with ethyl acetate, concentrating and drying the extracting solution to obtain lipiarmycin A4 coarse powder; C, dissolving the lipiarmycin A4 coarse powder in an organic solvent, filtering with a 0.45 mum filter membrane to obtain a sample loading solution; and D, adding the sample loading solution into a medium pressure chromatography column filled with C18, adding an elution solvent, collecting eluant with the content greater than or equal to 98.5% by high performance liquid chromatography, concentrating, filtering and drying in vacuum to obtain a high purity lipiarmycin A4 product. The high purity lipiarmycin A4 preparation method has the advantages of simple operation, high sample recovery rate, and suitability for high purity antagonizing drug-resistance antibiotic production.

The invention relates to a method for measuring trace lead in water by using a sulfydryl paper enrichment and separation colorimetric method; the method comprises the following steps of preparing sulfydryl paper (1), preparing a sulfydryl grafting liquid and 60.0 ml of mercaptoacetic acid, adding 36.0ml of acetic anhydride, 24.0 ml of glacial acetic acid and 10 drops of concentrated sulfuric acid,uniformly shaking and sealing for preservation in a dark place; and performing developing on a sulfydryl paper analysis solution enriched with lead in water, and carrying out detection. The method provided by the invention has the advantages of high enrichment rate and no leaching operation in determination, the problem that the sample is diluted due to leaching operation in a sulfydryl cotton enrichment method is overcome, and the sensitivity of the test is improved.

The invention relates to a specific gene segment suitable for real-time fluorescent quantitative PCR detection of pseudomonas PPZ-1 as well as primers of the specific gene segment and applications of the primers. For the specific gene segment suitable for real-time fluorescent quantitative PCR detection of pseudomonas PPZ-1, the nucleotide sequence is as shown in SEQ ID NO.1; the invention further relates to detection primers designed by utilizing the specific gene segment and applications of the detection primers. According to the technical scheme provided by the invention, the distribution of pseudomonas PPZ-1 can be tracked in real time, and thus the demand of biological repair monitoring is satisfied; according to actual detection, background noises can not be detected in a polluted sample in which pseudomonas PPZ-1 is not fed.

The invention relates to the technical field of tobacco detection and analysis and discloses an HPLC (High Performance Liquid Chromatography)-ICP (Inductively Coupled Plasma) method for quantitatively analyzing the content of chromium in a paper for a cigarette. The content of the chromium in the paper for the cigarette includes the content of hexavalent chromium and the content of trivalent chromium. With the adoption of the HPLC-ICP method, a sample is pretreated to obtain a sampling liquid, sampling analysis is directly carried out on the sampling liquid, the interference of a mass spectra and the interference of the trivalent chromium contained in the paper for the cigarette to the detection analysis of the hexavalent chromium are effectively eliminated, a high performance liquid chromatography and an inductively coupled plasma mass spectrometry condition are accurately limited, and the content of the hexavalent chromium is accurately analyzed; the pretreatment and complexation are carried out on the sample to obtain the sampling liquid, and the contents of the trivalent chromium and the hexavalent chromium in the paper for the cigarette are quantitatively analyzed and determined. The HPLC-ICP method has the advantages of low detection limit, high sensitivity, high recovery, good repeatability, short analysis time and the like and provides a strong technical base for determining the trivalent chromium and the hexavalent chromium in the mass samples.

The invention discloses a polyimidazole ionic liquid supported silica gel and a preparation method thereof. The polyimidazole ionic liquid supported silica gel comprises, by weight, 20-25 parts of a vinylimidazole monomer, 20-25 parts of a vinylimidazole acid radical ionic liquid, 35-45 parts of a silane coupling agent, 0.01-0.06 part of an initiator, 5-10 parts of a drying agent, 5-500 parts of asolvent, 2-12 parts of spherical silica gel, 0.01-0.3 part of a catalyst and 100-1000 parts of a washing liquid. The polyimidazole ionic liquid supported silica gel is applied to the field of adsorption desulfurization, and has the advantages of firmness and uniformity in adsorption, high activity, realization of repeated adsorption and desorption, good utilization rate is good, and realization of remarkable effect after repeated use.

The invention provides a distributed fiber sensing disturbance event detection method and apparatus. The distributed fiber sensing disturbance event detection method includes the steps: preprocessingobtained fiber sensing signals, and obtaining a first signal; according to the first signal, obtaining a second signal, wherein the second signal is a windowing signal; according to the second signal,calculating the short time energy and the corrective zero-crossing rate of the second signal; according to the mean value of the short time energy and the zero-crossing rate of the second signal, obtaining a reference standard deviation; and comparing the short time average energy of a disturbance signal to be detected and the zero-crossing rate standard deviation with the reference standard deviation, so as to determine whether disturbance exists. The distributed fiber sensing disturbance event detection method and apparatus have the advantages of effectively rejecting interference, greatlyreducing the rate of false alarm, achieving the technical effect with relatively higher detection probability, and solving the technical problem that false zero-crossing causes large rate of false alarm of the system.

The invention discloses a tetanus toxoid resisting monoclonal antibody, a hybridoma cell strain for producing the antibody, and an application of the antibody. The monoclonal antibody is secreted by the hybridoma cell strain WV-TT-05 with a preservation number of CCTCC NO:C2014200. The monoclonal antibody is used for specific detection of tetanus toxoid. The characteristics of being capable of removing interference of other proteins and detecting the tetanus toxoid specially and sensitively with high throughput are shown in the application of detecting the content of the tetanus toxoid in combined vaccines with the tetanus toxoid as a component and in coupling vaccines with the tetanus toxoid as a carrier, and the detection sensitivity reaches the nanogram level. Moreover, the operation is easy; the high specificity is achieved; and the detection sensitivity is improved.

The invention provides a system and a method for detecting methyl bromide gas concentration by optosonic spectrometry. The system comprises an antimonide laser light source, a chopper, an optosonic pond, a rheomicrophone and a signal processing unit. The antimonide laser light source is connected to the chopper and is arranged on one side of the optosonic pond; the rheomicrophone is arranged at the bottom of the optosonic pond, the laser light source emits light to the chopper, the chopper chops the light to form modulated light; the antimonide laser light source emits light to form a narrow-bond modulated spectrum to reach the optosonic pond. The system has the advantages of high sensitivity, high stability, good shield interference and small size. According to the principle of measuringmethyl bromide by optosonic spectrometry, no chemical substances are added, and the method is stable in optical structure after calibration primarily after delivery without secondary or multi-time calibration, so that the defects of a conductivity method are also avoided.