4 results about "Rheumatoid factor" patented technology

The present invention relates to the technical field of biological detection, and discloses an interleukin chemiluminescence detection kit and a detection method thereof, the kit comprises: interleukin magnetic particles coated with an interleukin antibody, the use concentration of the interleukin magnetic particles being 0.1-1 mg / mL; the interleukin luminous marker is an interleukin labeled antibody labeled by acridinium ester, and the use concentration of the interleukin luminous marker is 0.01 to 5 [mu] g / mL; and the interleukin analysis buffer solution contains a composite anti-interference component and is used for inhibiting the non-specific reaction of endogenous interfering substances in the sample to be detected. According to the present invention, the composite anti-interference component is introduced into the analysis buffer solution, such that the non-specific reaction of the heterotropism antibody, the human anti-mouse antibody, the rheumatoid factor and other endogenous interfering substances in the sample can be effectively inhibited, the detection background signal and the false positive rate are significantly reduced, and the detection accuracy under the complex sample matrix is improved.

The application discloses a method for constructing a spontaneous rheumatoid arthritis animal model, and the rheumatoid arthritis phenotype of the mouse is spontaneously generated by knocking out PPARgamma in the skin keratinocytes of the joint part, including joint swelling, joint synovial thickening, inflammatory cell infiltration, anti-cyclic citrullinated polypeptide antibody increase, and rheumatoid factor increase, so that the clinical symptoms of the rheumatoid arthritis are simulated, and an important tool is provided for the related research of the rheumatoid arthritis.

The present application relates to a kind of preparation methods of stable rheumatoid nephrology composite calibration quality control raw materials, comprising the following steps;Step 1, preparation composite quality control matrix liquid;Step 2, preparation quality control raw material additive;Step 3, the prepared quality control raw material additive is mixed into composite quality control matrix liquid;Step 4, the mixed solution is filtered and can be used.The present application uses rheumatoid factor (RF), C-reactive protein (CRP), anti-streptolysin (ASO), alpha 1 microglobulin (alpha 1-mg), beta 2 microglobulin (beta 2-mg) high-purity antigen as raw material, through composite debugging and assignment, and liquid protective agent (Tween 80, trehalose, anhydrous calcium chloride, ETDA, glycerol) is added, to obtain a kind of stable rheumatoid nephrology composite calibration quality control raw material.The rheumatoid nephrology composite calibration quality control raw material prepared has good stability, simple preparation process, low cost, high process repeatability, can be industrialized production, has potential and extensive application value in clinical diagnosis, in vitro diagnosis.

The invention provides a homogeneous chemiluminescence determination kit and a quantitative determination method of pepsinogen I. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a luminescence donor molecule coupled with a first antibody of pepsinogen I and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule coupled with a pepsinogen I second antibody and a reaction buffer solution. A donor / receptor double-labeled antibody sandwich reaction is adopted, one-step incubation is achieved, solid-phase separation and washing are not needed, the process is remarkably simplified, and the detection time is shortened; by collecting the luminous intensity at 620 nm and 665 nm and quantifying the luminous intensity by a specific value signal, errors caused by sample turbidity, color and volume fluctuation and instrument drift can be effectively corrected; meanwhile, a protein blocking agent, a chelating agent and a heterophilic antibody blocking component are introduced into a reaction buffer system, interference of non-specific adsorption and rheumatoid factors / heterophilic antibodies is reduced, and adaptability to complex matrixes such as bile fluid and serum / plasma is enhanced.