Latex enhanced immunoturbidimetry kit capable of inhibiting rheumatoid factor interference

A rheumatoid factor and latex enhancement technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of complex reagent preparation process, loss of antibody activity, and increased reagent cost, so as to achieve wide versatility, eliminate interference, compose simple effects

Inactive Publication Date: 2017-07-14
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, part of the antibody will be lost in the enzyme digestion and purification process of IgG, which will also affect the activity of the antibody to a certain extent, increasing the cost of the reagent, and more imp...

Method used

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  • Latex enhanced immunoturbidimetry kit capable of inhibiting rheumatoid factor interference
  • Latex enhanced immunoturbidimetry kit capable of inhibiting rheumatoid factor interference
  • Latex enhanced immunoturbidimetry kit capable of inhibiting rheumatoid factor interference

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: test in β2 microglobulin assay kit

[0031] 1. β2 Microglobulin Assay Reagent 1 Containing Different Amounts of NHS

[0032] β2 microglobulin assay reagent 1-A: 25mmol / L phosphate buffer, 0.9g / L sodium chloride, 0.5mg / L goat anti-human IgM antibody, 0.1g / L biological preservative Proclin300, 1g / L surface Active agent TX100, 0g / LNHS;

[0033] β2 Microglobulin Determination Reagent 1-B, C, D, E, F, G, H and I have the same components as Reagent 1-A, except that the concentration of NHS is different, they are 1g / L NHS, 5g / L respectively NHS, 10g / L NHS, 20g / L NHS, 30g / L NHS, 40g / L NHS, 45g / L NHS and 50g / L NHS.

[0034] 2. β2 microglobulin assay reagent 2: 20mmol / L glycine buffer, 0.2g / L latex particles sensitized by anti-human β2 microglobulin polyclonal antibody, 0.1g / L biological preservative Proclin300, 1g / L surface activity Agent TX100, 1g / L bovine serum albumin, 1g / L trehalose.

[0035] 3. Preparation of β2 microglobulin samples with different conte...

Embodiment 2

[0041] Embodiment two: test in cystatin C assay kit

[0042] 1. Cystatin C assay reagents containing different amounts of NHS 1

[0043] Cystatin C assay reagent 1-A: 200mmol / L phosphate buffer, 120g / L sodium chloride, 200mg / L goat anti-human IgM antibody, 0g / L NHS, biological preservative Proclin300 5g / L, surfactant TX100 2g / L

[0044] The components in Cystatin C Determination Reagent 1-B, C, D, E, F, G, H and I are the same as those in Reagent 1-A, except that the concentration of NHS is different, they are 1g / L NHS, 5g / L respectively NHS, 10g / L NHS, 20g / L NHS, 30g / L NHS, 40g / L NHS, 45g / L NHS and 50g / L NHS.

[0045] 2. Cystatin C assay kit assay reagent 2: 200mmol / L glycine buffer, 5.0g / L latex particles sensitized by anti-human cystatin C polyclonal antibody, 5g / L biological preservative Proclin300, 2g / L Surfactant TX100, 5g / L bovine serum albumin, 200g / L trehalose.

[0046] 3. Preparation of cystatin C samples containing different rheumatoid factor contents: Add diffe...

Embodiment 3

[0052] Embodiment three: test in the hypersensitive C-reactive protein assay kit

[0053] 1. High Sensitive C-Reactive Protein Assay Kit Containing Different NHS Amount Assay Reagent 1

[0054] Hypersensitive C-reactive protein assay kit Assay reagent 1-A: 100mmol / L phosphate buffer, 30g / L sodium chloride, 50mg / L goat anti-human IgM antibody, 1g / L biological preservative Proclin300, 3g / L L Surfactant TX100, 0g / L NHS;

[0055] The components in Reagent 1-B, C, D, E, F, G, H and I are the same as those in Reagent 1-A, except that the concentration of NHS is different, they are 1g / L NHS, 5g respectively / L NHS, 10g / L NHS, 20g / L NHS, 30g / L NHS, 40g / L NHS, 45g / L NHS, and 50g / L NHS.

[0056]2. High-sensitive C-reactive protein assay kit Determination reagent 2: 50mmol / L glycine buffer, 0.5g / L latex particles sensitized by anti-human high-sensitive C-reactive protein polyclonal antibody, 1g / L biological preservative Proclin300 , 3g / L surfactant TX100, 3g / L bovine serum albumin, 3g...

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PUM

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Abstract

The invention provides a latex enhanced immunoturbidimetry kit capable of inhibiting the rheumatoid factor interference. The kit comprises a reagent A and a reagent B. The reagent A is a solution capable of promoting the reactions between antigens and antibodies. The reagent B is a latex particle dispersion liquid containing sensitizing antibodies. The reagent A contains N-hydroxysuccinimide (1 to 40 g/L). The provided kit can effectively eliminate the interference of rheumatoid factor in a latex enhanced immunoturbidimetry sample on the immune-detection results; the accuracy and precision of measurement are both improved, and the kit has very good versatility.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a latex-enhanced immune turbidimetric kit for inhibiting rheumatoid factor interference. Background technique [0002] At present, the detection methods of body fluid marker proteins mainly adopt immunological methods, such as enzyme-linked immunosorbent assay (Enzyme-Linked Immol / Lunosorbent Assay, ELISA), radioimmunoassay (Radioimmol / Lunoassay, RIA) and colloidal gold chromatography (commonly known as " Gold Standard") These methods are the main ones. Although the ELISA method has been used clinically for nearly two decades, it still has some fatal shortcomings, such as poor quantitative accuracy, long operation time, and low degree of automation. Generally, it can only be used for qualitative detection. RIA has high sensitivity, but it is unstable, its repeatability is worse than that of ELISA, and there is a risk of radioactive contamination. Although the gold st...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/544
CPCG01N33/54313G01N33/54393G01N33/544G01N33/68G01N33/721
Inventor 耿英利罗湘宇甘萍萍
Owner SICHUAN MACCURA BIOTECH CO LTD
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