The invention relates to a chimeric antigen receptor targeting to CD19-41BB-tEGFR, and application thereof. Specifically, the invention provides a polynucleotide sequence. The polynucleotide sequenceis selected from (1) a polynucleotide sequence containing, successively connected, a coding sequence of an anti-CD19 single-chain antibody, a coding sequence of the hinge region of human CD8alpha, a coding sequence of the transmembrane region of human CD8, a coding sequence of the intracellular region of human 41BB, a coding sequence of the hinge region of human CD8zeta and an optional coding sequence of an EGFR fragment containing an extracellular domain III and an extracellular domain IV; and (2) complementary sequences of the polynucleotide sequence described in (1). The invention also provides related fusion proteins, vectors containing the coding sequences, and application of the fusion proteins, the coding sequences and the vectors.