The invention discloses a method for promoting
cell proliferation and activation of
peripheral blood CD4+
T lymphocyte and suppressing
cell proliferation and migration of
acute lymphocytic leukemia (ALL) Jurkat T cells by using red
cassia tree
lectin. The separation and purification of red
cassia tree
lectin from red
cassia tree seeds can be carried out at the temperature of 1-5 DEG C during the entire process, the proliferation and activation of the
peripheral blood
T lymphocyte CD4 subgroup can be promoted by the red cassia tree
lectin through the verifications of an MTT (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide) experiment,
a DNA (deoxyribonucleic acid) analysis and an FCM (
flow cytometry), and the red cassia tree lectin is CD4+
T lymphocyte mitogen more efficient than PHA (Phytohaemagglutinin) and provides a new and effective way for anti-
virus infection and vaccine
intensifier development; through the verifications of CCK-8 (
Cell Counting Kit-8) experiment,
DNA analysis,
cell apoptosis analysis, ELISA (
enzyme-linked immuno
sorbent assay) experiments and migration
assay, the red cassia tree lectin can obviously suppress the proliferation and migration of the Jurkat T
lymphocyte, is a growth inhibitor more efficient for the ALL cells and provides a new and effective supplementary means for treating the hematological malignancies.