The invention discloses a production method of
bacterial exotoxin, comprising the procedures of bacterial
toxin production culture,
bacterial exotoxin extraction,
bacterial exotoxin purification and bacterial
exotoxin preservation, wherein the bacterial
toxin production culture is carried out in a
microorganism dialysis incubator which comprises a culture medium chamber and a culture chamber, a
dialysis membrane and a
dialysis membrane supporting and protecting device are arranged between the culture medium chamber and the culture chamber, the ratio of the volume of the culture medium chamber to the total volume of the culture chamber is selected between 3:1 and 1:6 according to a
microorganism culture time, and facilities needed by
microorganism growth are arranged in the culture medium chamber. The production method comprises the procedures of
toxin production culture, sterilization,
filtration, concentration,
precipitation, percrystallization and preservation. The production method of bacterial
exotoxin is adopted to produce toxin, optimizes
bacterial growth environment, simplifies the technologies of concentration and purification of bacterial
exotoxin, increases the yield to 90 percent from 17-33 percent, and shortens the purification time to about 12-24 hours from the original natural dialysis of 12-15 days.