140 results about "Endoplasmic reticulum" patented technology

The invention discloses a method for efficiently expressing laccase by regulating endoplasmic reticulum stress reaction of coprinus cinereus, and belongs to the field of gene engineering and fermentation engineering. According to the invention, by regulating endoplasmic reticulum stress reaction action genes der1, Anhac1 and Cchac1 of filamentous fungi coprinus cinereus, recombinant extracellular expression of laccase Lcc5 is finally improved and regulated. Wherein the extracellular laccase activities of the recombinant bacteria can be respectively improved by 18%, 70% and 26% through interference of der1, overexpression of Anhac1 and overexpression of Cchac1, and the obtained extracellular laccase activities are respectively 31.4 U / mL, 45.1 U / mL and 33.6 U / mL, which is the highest level of coprinus cinereus homologous recombination expression laccase Lcc5 reported at present. The invention firstly indicates that the regulation and control of the coprinus cinereus endoplasmic reticulum stress reaction can promote the efficient expression of laccase in coprinus cinereus, and the invention further promotes the development of a coprinus cinereus expression system.

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

To provide an endoplasmic reticulum stress suppressing composition and a liver function improving composition.SOLUTION: A composition for suppressing endoplasmic reticulum stress and / or a composition for improving hepatic functions, comprising one or more plants selected from the group consisting of Plantago asiatica, Hamilton's spindle tree, panicled hydrangea, Japanese plum yew, Japanese mallotus, and Papaver rhoeas, and / or an extract thereof.SELECTED DRAWING: None

The invention provides an AIE photosensitizer with an endoplasmic reticulum targeting function as well as a preparation method and application of the AIE photosensitizer. The AIE photosensitizer has the following structure shown in the specification. Under the action of illumination, TTP-ER can induce generation of a large amount of ROS in the toxoplasma gondii bodies, typical ultrastructure damage such as cell membrane rupture and collapse is caused, and rapid inactivation of the free toxoplasma gondii is achieved. Meanwhile, the photosensitizer treatment can significantly inhibit the formation of plaques of polypide bodies in host cells, reduce the invasion, replication and multiplication capacities, and show clear in-vitro toxoplasma gondii resistance activity.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PoPILS1a gene in regulation and control of plant seed development. The coding sequence of the PoPILS1a gene is as shown in SEQ ID NO: 1. According to the invention, the specific expression of the peony PoPILS1a in seed tissues is found for the first time, the expression levels are greatly different in different development stages, and the PoPILS1a protein is positioned on a cell endoplasmic reticulum. In an arabidopsis thaliana positive strain with over-expression of PILS1a, the epiphytic point between pods is obviously shortened, the number of pods with limited inflorescence length distribution is larger, meanwhile, the lengths and the weights of the pods at the corresponding positions are obviously different compared with those of a contrast, and it is clear that PILS1a can promote development of fruits and has good practical application value.

The invention discloses a nano-particle for inducing death of tumor immunogen cells, a preparation method and application. The preparation method of the nano-particles comprises the following steps: taking a BiOCl nanosheet as a carrier, enabling CeO2 to generate and crystallize in situ on the surface of the BiOCl nanosheet, and forming a BiOC / CeO2 heterojunction; the method comprises the following steps: carrying out a dehydration reaction on NH2-PEG2000-NH2 and DL lipoic acid to synthesize NH2-PEG2000-TA; the preparation method comprises the following steps: reacting NH2-PEG (Polyethylene Glycol) 2000-TA with a Pardaxin peptide of which the carboxyl is activated, so as to generate Pardaxin-PEG 2000-TA; the preparation method comprises the following steps of: coupling Pardaxin-PEG2000-TA (Polyethylene Glycol 2000-TA) and a BiOC / CeO2 heterojunction; the PBCP nanoparticles obtained by the preparation method promote the yield of active oxygen in the endoplasmic reticulum of tumor cells to be increased sharply, so that the endoplasmic reticulum stress is effectively excited, tumor immunogenic cells are induced to die, and the anti-tumor effect is achieved. The nanoparticle can be combined with the existing PD-1 / PD-L1 inhibitor for use, and shows a better anti-tumor effect than the single use of the PD-1 / PD-L1 inhibitor.

The invention belongs to the technical field of biological medicine and molecular biology, and particularly relates to application of MIGA2 serving as a target spot in screening or preparing a medicine for treating colorectal cancer. According to the invention, the correlation between MIGA2 and colorectal cancer progress is analyzed through large-queue bioinformatics; the influence of MIGA2 on the proliferation, migration and invasion ability of colorectal cancer cells is analyzed from an in-vitro cell model; analyzing a key molecular mechanism of MIGA2 playing a cancer inhibition function; the influence of MIGA2 on colorectal cancer tumor growth is analyzed in an in-vivo mouse model, the influence of MIGA2 mediated mitochondrial-endoplasmic reticulum contact on colorectal cancer tumor growth is analyzed, and it is verified in multiple dimensions that MIGA2 can become an effective treatment target of colorectal cancer.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

Levels of expression of antibiotic resistance genes are increased up to six-fold by inserting a proteasome-targeting tag into transgenes expressed in eukaryotic cells. Various selectable marker proteins are combined with different destabilization domains, leading to up to 70% increase in transgene expression. The increase in expression varies highly depending on the engineered construct and the lines cells used. Increase in expression drives exosome loading of cargo proteins in some aspects. By increasing expression and by editing trafficking signals of cargo proteins, proteins that normally locate to the ER can be trafficked to exosomes. This disclosure discloses efficient exosome delivery of a wide variety of engineered proteins, including modified antigen proteins of SARS-CoV-2 and influenza, and other proteins such as a modified alpha galactosidase A, an extracellular domain of vascular endothelial growth factor fused to a constant region of a human immunoglobulin heavy chain, and modified trastuzumab heavy and light chains.

The invention discloses a cascade targeting ruthenium (II) complex as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, preparing a ligand I; step 2, preparing a ligand 2; and step 3, preparing a cascade targeting ruthenium (II) complex: preparing a metal ruthenium complex through the ligand I, and carrying out coordination on the metal ruthenium complex and the ligand II to obtain the cascade targeting ruthenium (II) complex. The cascade targeting ruthenium (II) complex is novel in structure, has tumor targeting and endoplasmic reticulum targeting properties, can be used as a photosensitizer, and can generate active oxygen under illumination for photodynamic therapy of tumors. The target cascade targeting type ruthenium (II) complex is applied to preparation of anti-cancer drugs and preparation of type II ICD inducer drugs. The compound not only has excellent anti-tumor activity, but also can be used as a type II ICD inducer to induce tumor cells to generate ICD; the preparation yield is high; the repeatability is good and the like; good biocompatibility is achieved, and clinical use is facilitated.

The invention belongs to the technical field of biological medicines, and particularly discloses a pork liver source / photosensitizer-loaded pork liver source lipid nanoparticle and application thereof in endoplasmic reticulum stress enhanced phototherapy. The lipid extraction method provided by the invention has the advantages of mature process, low cost, easily available raw materials, clear components of the obtained lipid, and high biocompatibility. The pork liver source lipid nanoparticles prepared from natural lipid extracted from pork liver have good endoplasmic reticulum targeting property, and can be used as a carrier to efficiently load a photosensitizer IR780; the related nanoparticle preparation method is simple, good in repeatability and easy for expanded production. A phototherapy system constructed by the nanoparticles can generate active oxygen in situ in an endoplasmic reticulum under near-infrared laser irradiation to initiate strong endoplasmic reticulum stress, so that the anti-tumor effect of photothermal and photodynamic combined treatment is remarkably enhanced, and the nanoparticles can be widely applied to efficient and accurate treatment of various solid tumors.

The application discloses a kind of nanoclusters with golgi targeting and its preparation method and application, the nanocluster is zinc sulfide nanocluster, the surface of the zinc sulfide nanocluster is coupled with targeting ligand, the targeting ligand can actively target RA synovial activated macrophage, and can target golgi subcellular organelle, the nanocluster can release H2S gas in inflammatory weak acid microenvironment.The application can realize "inflammatory joint enrichment-> macrophage active uptake-> lysosome escape-> golgi deep anchoring" multi-level precise delivery, release H2S gas in inflammatory weak acid microenvironment, fundamentally reshape intracellular Ca 2+ Steady state, relieve endoplasmic reticulum, golgi and mitochondria triple stress, block inflammatory signal pathway, realize the efficient, safe treatment of RA.

The present invention relates to a composition for protecting cells from endoplasmic reticulum (ER) stress and oxidative stress induced by ultrafine dust, comprising galangin as an active ingredient. Galangin, which is an active ingredient of the composition for cell protection of the present invention, alleviated an increase in reactive oxygen species, intracellular calcium, and ER stress-related proteins induced by ultrafine dust PM2.5. In addition, galangin counteracted PM2.5-induced cell cycle arrest at the G0 / G1 phase and restored cell cycle regulators and matrix metalloproteinases. As a result, galangin can enhance cell protection against PM2.5-induced ER stress and cell cycle arrest via reactive oxygen species. Therefore, it is suggested that the composition comprising galangin as an active ingredient of the present invention has great potential as a candidate for a preventive agent or a therapeutic agent for diseases related to ER stress and oxidative stress induced by PM2.5.

The MYC and NMYC transcription factors (TFs) play a key role in cell proliferation and are overexpressed in most cancer cells. However, in normal cells their overexpression triggers safeguard mechanisms promoting cell death and cellular senescence, which are bypassed in cancer cells. Here, the inventors reveal that in normal cells MYC binds to the Inositol 1,4,5-Trisphosphate Receptor type 1 (ITPR1) gene and upregulates its expression, triggering an ER-mitochondria calcium (Ca2+) transfer, which is involved in MYC-induced cell death and senescence. Supporting a tumor suppressive role of MYC / ITPR1 axis, ITPR1 expression is generally decreased in cancer and reactivation of this pathway induces cancer cell death. Nevertheless, some cancer cells, generally expressing high levels of MYCN and / or MYC, also express high level of ITPR1, which correlates with high expression of BCL2, encoding an inhibitor of ITPR1. Strikingly, in high-risk MYCN-amplified neuroblastoma, ITPR1 expression is controlled by NMYC and its level correlates with worse patient survival. In these cells, blocking the interaction between BCL2 and ITPR1, via an BCL2-BH4 domain inhibitor induces mitochondrial Ca2+ accumulation and cell death, and decreases tumor size. Thus, the present invention relates to a method for treating MYChigh cancer, and in particular NMYC--amplified neuroblastoma in a subject by administering an BCL2-BH4 domain inhibitor.

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.

The application belongs to the technical field of feed additives, discloses a compound functional additive for relieving liver oxidative stress and endoplasmic reticulum stress of mandarin fish, and specifically discloses a feed additive which comprises the following components: sodium octanoate, sodium butyrate and rice husk powder. The additive provided by the application can effectively relieve liver oxidative stress and endoplasmic reticulum stress of mandarin fish, reduce the content of malondialdehyde, improve the ability of mandarin fish to resist oxidative enzyme activity, and is beneficial to the health of fish body.

Provided herein is a method of treating liver dysfunction in a patient, comprising administering to the patient a composition that reduces stress in the endoplasmic reticulum in an amount effective to treat the dysfunction in the patient.

This invention discloses a genetically engineered bacterium for synthesizing carotenoids, its construction method, and its applications, belonging to the field of genetic engineering technology. The genetically engineered bacterium uses a carotenoid-producing yeast strain as the starting material. The gene ROX1, encoding a heme-dependent hypoxia gene repressor, is knocked out, while the expression of genes STB5 (encoding a transcription factor involved in NADPH regeneration), DID2 (encoding a class E protein in the vacuole protein sorting pathway), and VOA1 (encoding an endoplasmic reticulum protein playing a role in the assembly of the V0 region of V-ATPase) is upregulated. This invention promotes carotenoid synthesis and significantly increases carotenoid yield through combined regulation of gene expression outside the carotenoid synthesis pathway, demonstrating promising application prospects.

The invention discloses application of oxygen-carrying protein VHb in construction of high-yield astaxanthin engineering bacteria, and belongs to the technical field of biology. According to the method, carRPY27R, carB, fusion genes crtW-crtZ and GGPPS are iteratively integrated onto a yarrowia lipolytica chassis genome by utilizing a synthetic biological technology, so that two recombinant strains for producing astaxanthin are obtained. On the basis, the oxygen-carrying protein VHb from vitreoscilla is expressed, and compared with the oxygen-carrying protein VHb which is not expressed, the VHb is positioned to the endoplasmic reticulum, so that the accumulation level of astaxanthin is improved by 272.99%, and the biomass is improved by 94.15%. According to the method, the oxygen-carrying protein VHb is expressed on cytoplasm and organelle, so that the synthesis efficiency and biomass of astaxanthin are remarkably improved. In addition, the intracellular dissolved oxygen level is improved, so that the cost for improving the dissolved oxygen in the fermentation process is reduced.

The present invention relates to a method for treating cancer, comprising administering a composition comprising γδ T cells treated with an inhibitor of XBP1 gene expression or an inhibitor of XBP1 protein expression or activity. The γδ T cells, in which the XBP1 gene is deficient or the XBP1 protein activity is inhibited, exhibit enhanced antitumor activity in a tumor microenvironment characterized by endoplasmic reticulum (ER) stress, as compared to unmodified γδ T cells. Accordingly, the modified γδ T cells are effective for use in cancer treatment.

The invention relates to the technical field of biological medicines, in particular to gold nanoparticles modified by arylamine derivatives as well as a preparation method and application of the gold nanoparticles. The gold nanoparticles modified by the arylamine derivatives provided by the invention can improve the endoplasmic reticulum stress level of non-small cell lung cancer cells, can also improve the sensitivity of the non-small cell lung cancer cells to chemotherapeutic drugs, and can improve the death rate of the non-small cell lung cancer cells in cooperation with the chemotherapeutic drugs.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The invention relates to a multi-dimensional anti-aging composition suitable for sensitive skin and application, and belongs to the technical field of cosmetics. The multi-dimensional anti-aging composition suitable for sensitive skin is prepared from a Burbury leaf extract, a rice extract, an ulmus pumila bark extract and an anise leaf extract, and the four components are reasonably compounded. The synergistic anti-aging effect is achieved from a plurality of levels of regulating DNA methylation from a gene level, relieving endoplasmic reticulum stress from a cell level, maintaining collagen synthesis and degradation balance from a metabolism level, strengthening a skin barrier and the like.

This article provides novel signal peptides for guiding encoded proteins to the endoplasmic reticulum of cells, recombinant polypeptides comprising said signal peptides, nucleic acid molecules encoding said recombinant polypeptides, pharmaceutical compositions comprising said nucleic acid molecules, and methods of using the same.

The present invention relates to food and pharmaceutical compositions that alleviate endoplasmic reticulum stress and thereby suppress or prevent various diseases caused by endoplasmic reticulum stress. More specifically, the present invention relates to food and pharmaceutical compositions for preventing or treating symptoms of fatty liver or hepatitis, which contain a lysate of any one selected from the group consisting of Saccharomyces celloviciae KCTC13925BP, KCTC14122BP, KCTC14123BP, KCTC14983BP, KCTC14984BP, and KCTC14985BP, or a mixture thereof.

Methods and compositions for enhancing heterologous protein expression in plants are provided. The invention is directed to the co-expression of a heterologous protein of interest with a specific endoplasmic reticulum (ER)-resident molecular chaperone protein. Co-expressing chaperones such as a native soybean protein disulfide isomerase-like protein (Glyma.01G003700, Glyma.04g247900, Glyma.03G218300, or Glyma.18G204000), or a heterologous mammalian chaperone (bovine DNAJB12) with casein proteins in soybean cells resulted in a significant increase in casein accumulation, with up to 4-fold enhancement observed. The methods are broadly applicable for improving the yield of complex recombinant proteins, including milk proteins, therapeutic proteins, and industrial enzymes, in plant-based production systems, thereby overcoming common limitations related to protein folding and stability.

The invention discloses application of MYSM1 regulation and control of ITPR1 mediated cell autophagy in inhibition of cervical cancer, and belongs to the technical field of biomedicine.The MYSM1 gene and / or expression or activity of encoded protein of the MYSM1 gene are / is up-regulated, and an MYSM1-ITPR1-autophagy signal channel is activated, so that proliferation, migration, invasion or epithelial-mesenchymal transition of cervical cancer cells is inhibited, and cervical cancer is inhibited. And / or promoting apoptosis of cervical cancer cells; wherein the MYSM1 activates the expression of ITPR1 through the activity of a deubiquitination enzyme which is subjected to single ubiquitination modification on the 119th lysine of histone H2A of the MYSM1, and the ITPR1 serves as an endoplasmic reticulum calcium ion channel to mediate calcium ion release so as to trigger cell autophagy. Based on the mechanism, the invention provides a new application of MYSM1 / ITPR1 in preparation of anti-cervical cancer drugs, a drug composition targeting the pathway, a candidate drug screening method and a kit for diagnosis and prognosis. The invention aims to solve the problem that a technical scheme for effectively inhibiting the malignant progression of cervical cancer by targeting an MYSM1-ITPR1 pathway is lacked in the prior art.

The application relates to the field of biotechnology, and particularly relates to the application of carnosic acid as an ERAP1 inhibitor in the preparation of a drug for treating ankylosing spondylitis of an autoimmune disease. The application first discovers that a monomer compound carnosic acid from a natural product source can be used as an ERAP1 direct targeting inhibitor in the preparation of a drug for regulating an endogenous antigen processing and presentation pathway and ankylosing spondylitis of a related autoimmune disease. The carnosic acid can be specifically combined in an ERAP1 active site through direct high-activity targeting, and the carnosic acid inhibits the activity of the ERAP1, is the highest in activity in the world, and has high selectivity in targeting the ERAP1 active site. The carnosic acid reverses the ankylosing spondylitis molecular pathological phenotypes caused by abnormal ERAP1 activity, including endoplasmic reticulum stress, reducing an extra amount of HLA-B27 molecules displayed on a cell surface, reducing the proportion of the HLA-B27 molecules incorrectly assembled on the cell surface, and maintaining normal antigen presentation functions.

The crystalline form of the compound of formula (I) and its pharmaceutical composition are provided herein. Methods of using the crystalline form and pharmaceutical composition to treat cancer and viruses (e.g., coronavirus), as well as other diseases and disorders involving protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), are also provided herein. [Formula 1] JPEG2026513203000160.jpg23170

The present invention relates to a composition for alleviating skin damage caused by particulate matter (PM), the composition comprising myricetin as an active ingredient. Myricetin, which is an active ingredient of the composition for alleviating skin damage according to the present invention, can effectively reduce oxidative stress caused by PM2.5, endoplasmic reticulum stress and mitochondrial dysfunction, and apoptosis caused by cell exposure to PM2.5. Therefore, the composition comprising myricetin as an active ingredient according to the present invention shows great potential as a therapeutic candidate for treating skin damage caused by PM2.5.