Latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference
A rheumatoid factor, latex enhancement technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of increasing the cost of reagents, losing the activity of antibodies and antibodies, and restricting promotion, and achieves the effect of eliminating interference and weakening non-specific binding reactions.
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Embodiment 1
[0014] Embodiment one is directed at ferritin detection reagent (latex-enhanced immune turbidimetry)
[0015] Ferritin detection reagent R2 (pH7.4): 10mM PB buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.1% ferritin polyclonal antibody sensitization nano latex particles.
[0016] Ferritin sample preparation with different rheumatoid factor contents:
[0017] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 300 μg / L pure ferritin. There were two sources of rheumatoid factor, purchased from two different manufacturers (RF-Ⅰ, RF-Ⅱ ).
[0018] (1) Comparison of ferritin detection reagent R1 with different pH values
[0019] Ferritin detection reagent R1-A (pH7.4): 10 mM PB buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 1% PEG6000.
[0020] Ferritin detection reagent R1-B (pH6.0): 100 mM MES buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 1% PEG6000.
[0021] Ferritin detection reagent...
Embodiment 2
[0040] Embodiment two pepsinogen Ⅰ detection reagent (latex-enhanced immune turbidimetric method)
[0041] Pepsinogen Ⅰ detection reagent R2 (pH7.5): 20mM Tris buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.15% pepsinogen Ⅰ single Cloning of antibody-sensitized latex nanoparticles.
[0042] Preparation of pepsinogen Ⅰ samples with different rheumatoid factor contents:
[0043] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 100 μg / L of pure pepsinogen Ⅰ. There were two sources of rheumatoid factor, which were purchased from two different manufacturers (RF-Ⅰ, RF- II).
[0044] (1) Comparison of pepsinogen Ⅰ detection reagent R1 with different pH values
[0045] Pepsinogen Ⅰ detection reagent R1-A (pH7.4): 100mM MES buffer, 300mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 0.5% PEG6000.
[0046] Pepsinogen Ⅰ detection reagent R1-B (pH6.0): 100mM MES buffer, 300mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide...
Embodiment 3
[0066] Embodiment three is aimed at procalcitonin detection reagent (latex-enhanced immune turbidimetric method)
[0067] Procalcitonin detection reagent R2 (pH7.5): 20mM Tris buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.1% procalcitonin Cloning of antibody-sensitized latex nanoparticles.
[0068] Preparation of procalcitonin samples with different rheumatoid factor contents:
[0069] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 0.5ng / mL of pure procalcitonin. There were two sources of rheumatoid factor, which were purchased from two different manufacturers (RF-I, RF -II).
[0070] (1) Comparison of procalcitonin detection reagent R1 with different pH values
[0071] Procalcitonin detection reagent R1-A (pH7.4): 10mM PB buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 2% PEG6000.
[0072] Procalcitonin detection reagent R1-B (pH6.0): 100 mM MES buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% ...
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