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Latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference

A rheumatoid factor, latex enhancement technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of increasing the cost of reagents, losing the activity of antibodies and antibodies, and restricting promotion, and achieves the effect of eliminating interference and weakening non-specific binding reactions.

Active Publication Date: 2015-10-14
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, part of the antibody will be lost in the enzyme digestion and purification process of IgG, which will also affect the activity of the antibody to a certain extent, increasing the cost of the reagent, and more importantly, the preparation process of the reagent is further complicated, which sets an insurmountable obstacle for controlling the difference between batches of reagents. , thus limiting the clinical promotion of such reagents

Method used

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  • Latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference
  • Latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference
  • Latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment one is directed at ferritin detection reagent (latex-enhanced immune turbidimetry)

[0015] Ferritin detection reagent R2 (pH7.4): 10mM PB buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.1% ferritin polyclonal antibody sensitization nano latex particles.

[0016] Ferritin sample preparation with different rheumatoid factor contents:

[0017] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 300 μg / L pure ferritin. There were two sources of rheumatoid factor, purchased from two different manufacturers (RF-Ⅰ, RF-Ⅱ ).

[0018] (1) Comparison of ferritin detection reagent R1 with different pH values

[0019] Ferritin detection reagent R1-A (pH7.4): 10 mM PB buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 1% PEG6000.

[0020] Ferritin detection reagent R1-B (pH6.0): 100 mM MES buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 1% PEG6000.

[0021] Ferritin detection reagent...

Embodiment 2

[0040] Embodiment two pepsinogen Ⅰ detection reagent (latex-enhanced immune turbidimetric method)

[0041] Pepsinogen Ⅰ detection reagent R2 (pH7.5): 20mM Tris buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.15% pepsinogen Ⅰ single Cloning of antibody-sensitized latex nanoparticles.

[0042] Preparation of pepsinogen Ⅰ samples with different rheumatoid factor contents:

[0043] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 100 μg / L of pure pepsinogen Ⅰ. There were two sources of rheumatoid factor, which were purchased from two different manufacturers (RF-Ⅰ, RF- II).

[0044] (1) Comparison of pepsinogen Ⅰ detection reagent R1 with different pH values

[0045] Pepsinogen Ⅰ detection reagent R1-A (pH7.4): 100mM MES buffer, 300mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 0.5% PEG6000.

[0046] Pepsinogen Ⅰ detection reagent R1-B (pH6.0): 100mM MES buffer, 300mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide...

Embodiment 3

[0066] Embodiment three is aimed at procalcitonin detection reagent (latex-enhanced immune turbidimetric method)

[0067] Procalcitonin detection reagent R2 (pH7.5): 20mM Tris buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 5% trehalose, 0.1% procalcitonin Cloning of antibody-sensitized latex nanoparticles.

[0068] Preparation of procalcitonin samples with different rheumatoid factor contents:

[0069] 0, 20, 100, and 400 IU / mL of rheumatoid factor were added to samples containing 0.5ng / mL of pure procalcitonin. There were two sources of rheumatoid factor, which were purchased from two different manufacturers (RF-I, RF -II).

[0070] (1) Comparison of procalcitonin detection reagent R1 with different pH values

[0071] Procalcitonin detection reagent R1-A (pH7.4): 10mM PB buffer, 150mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% sodium azide, 2% PEG6000.

[0072] Procalcitonin detection reagent R1-B (pH6.0): 100 mM MES buffer, 150 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% ...

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Abstract

The present invention discloses a latex reinforced immunoturbidimetric reagent for inhibiting rheumatoid factor interference, wherein the latex reinforced immunoturbidimetric reagent comprises a reagent R1 and a reagent R2, the reagent R2 comprises latex micro-particles, and the PH value of the reagent R1 is 5.5-6.0. According to the present invention, the latex reinforced immunoturbidimetric reagent has characteristics of simple composition and good versatility, and with the latex reinforced immunoturbidimetric reagent, the interference of most of the rheumatoid factors can be effectively eliminated, and the specificity and the accuracy of the detection result can be improved.

Description

technical field [0001] The invention relates to an immune detection reagent, in particular to a latex-enhanced immune turbidimetric reagent capable of suppressing rheumatoid factor interference. [0002] 0.01-0.2% (W / V) refers to the addition of 0.01-0.2g of solute per 100ml of solvent, similar points in this paper agree with this point. Background technique [0003] Latex-enhanced immunoturbidimetry is a detection method developed on the basis of immunoturbidimetry to determine the content of special marker proteins in human body fluid. The basic principle is: coat the nano-latex microspheres with high specificity and high affinity antibodies corresponding to the substances to be tested. When the microspheres are mixed with the samples to be tested, under appropriate reaction conditions, the antibodies and The antigen in the sample is specifically combined to form an antigen-antibody-latex particle complex, which quickly gathers together in a short period of time, changing...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/544
CPCG01N33/54346G01N33/54393G01N33/544
Inventor 陈媛张闻许琴方蓉陈思思王建飞周海滨
Owner NINGBO RUI BIO TECH
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