IgM (immunoglobulin M) antibody detection test strip

An antibody detection and test strip technology, applied in the field of clinical diagnosis, can solve the problems of inability to realize automation, inconvenient application, cumbersome operation, etc., and achieve the effects of good removal effect, simple operation and improved accuracy.

Inactive Publication Date: 2012-06-20
无锡博慧斯生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this separation method is time-consuming, cumbersome to operate

Method used

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  • IgM (immunoglobulin M) antibody detection test strip
  • IgM (immunoglobulin M) antibody detection test strip
  • IgM (immunoglobulin M) antibody detection test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 adsorbent is coated on the nitrocellulose membrane

[0036] 1. Colloidal gold labeling

[0037]Antigen (Treponema pallidum specific antigen (TP47+15, TP17)) colloidal gold labeling method for syphilis diagnosis is as follows: adjust the pH value of 50ml colloidal gold solution to 7.7 with potassium carbonate solution; Add 0.65ml of 1mg / ml Treponemal pallidum specific antigen to be labeled into the above colloidal gold solution, mix well, and let stand at room temperature for 30min; slowly add 12.5ml blocking solution to the above colloidal gold solution, mix well, and let stand at room temperature for 30min; 8000g 4 Centrifuge at ℃ for 1 hour, remove the supernatant, and leave the loose precipitate; dissolve the precipitate in each tube with 25ml of washing solution, centrifuge at 6000g 4°C for 1 hour, remove the supernatant, and leave the loose precipitate; dissolve the precipitate in each tube with 1.2ml of washing solution, transfer to 1.5ml centrifuge ...

Embodiment 2

[0048] Example 2 The adsorption pad is located between the sample pad and the gold standard pad

[0049] 1. Colloidal gold labeling

[0050] With embodiment 1.

[0051] 2. Spray point of colloidal gold pad (combination pad)

[0052] With embodiment 1.

[0053] 3. Preparation of nitrocellulose membrane

[0054] The nitrocellulose membrane streaking method used for the diagnosis of syphilis is as follows:

[0055] Take Treponema pallidum-specific antigen (TP47+15, TP17) 500ug, add it to a 5ml graduated centrifuge tube, dilute the antigen to 1ml, and mark the container with a T mark. Take 25ul of mouse anti-human Treponema pallidum specific antigen (TP47+15, TP17) antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with C mark. Set the film spotting instrument, turn on the power of the film spotting instrument, set the spray point program, and the spray point volume is 1ul / cm; No. 1 pipeline is the spray point channel of th...

Embodiment 3

[0064] Example 3 The adsorption pad is located between the gold standard pad and the nitrocellulose membrane

[0065] 1. Colloidal gold labeling

[0066] With embodiment 1.

[0067] 2. Spray point of colloidal gold pad (combination pad)

[0068] With embodiment 1.

[0069] 3. Preparation of nitrocellulose membrane

[0070] The nitrocellulose membrane streaking method used for the diagnosis of syphilis is as follows:

[0071] Take 1mg of anti-human μ chain antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with a T mark. Take 25ul of mouse anti-human Treponema pallidum specific antigen (TP47+15, TP17) antibody, add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with C mark. Set the film spotting instrument, turn on the power of the film spotting instrument, set the spray point program, and the spray point volume is 1ul / cm; No. 1 pipeline is the spray point channel of the detecti...

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PUM

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Abstract

The invention relates to an IgM (immunoglobulin M) antibody detection test strip, comprising an IgM antibody detection line, wherein a matter capable of absorbing human IgM and rheumatoid factors is coated in front of the IgM antibody detection line. Preferably, the matter is an anti-huamn IgG antibody, Protein G and human IgG aptamer; both the IgM antibody detection line and the matter are both on a nitrocellulose membrane; or the matter is on an absorption pad in front of the IgM antibody detection line, and the absorption pad can be arranged between the nitrocellulose membrane and a combined pad, and also can be arranged between the combined pad and a sample pad. The IgM antibody detection test strip provided by the invention can simply and quickly detect the IgM antibody, obviously improve the detection accuracy, provide a powerful support for the next accurate treatment and is suitable for large-scale promotion and application.

Description

technical field [0001] The invention relates to the technical field of clinical diagnosis, in particular to the technical field of antibody detection test strips, in particular to an IgM antibody detection test strip. Background technique [0002] Immunoglobulin (Ig) is a globulin with antibody activity or chemical structure similar to an antibody; it is an important class of immune effector molecules, most of which are gamma globulins, consisting of two identical light chains and two Composed of the same heavy chain, it exists in two forms in the body: soluble immunoglobulin exists in body fluid, has antibody activity, and participates in humoral immunity; membrane immunoglobulin is a B cell antigen receptor. [0003] Immunoglobulins are produced by lymphocytes of the immune system of higher animals and can be converted into antibodies after being induced by antigens. Due to different structures, it can be divided into five types: IgG, IgA, IgM, IgD and IgE. [0004] The ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/532
Inventor 韦彦余雷震
Owner 无锡博慧斯生物医药科技有限公司
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