75 results about "Higher animals" patented technology

The invention provides a broad-spectrum rod-shaped viral pesticide produced by substitute hosts, the components and the weight proportion of which are: 20 billion PIB / ml virus suspension 2.5 to 15 percent, 60nm silicon dioxide 0.1 to 0.5 percent, aromatic amino acid 0.1 to 0.15 percent, refined fluorescent whitening agent 0.5 to 1 percent, sodium lignin sulfonate 0.1 to 1 percent, chlorfluazuron 0.01 to 0.1 percent, glycerol 1.0 to 2.0 percent, and remaining no sterilized water; the virus suspension is received by the infection of nuclear polyhedrosis virus of cabbage armymoth and the substation for the host---cotton bollworm or beet armyworm. The pesticide not only maintains the biological pesticide properties of viral pesticides and has no harms to human beings and higher animals, but also is suitable for the the massive employments on vegetables, and has the wide insect disinfestation spectrum. Therefore, a broad-spectrum viral pesticide can prevent and control various harmful insects while with only a rod-shaped virus. The invention has the advantages of good insecticidal effect, fast insecticidal speed, and very good application prospect.

The present invention discloses beet noctuid nuclear polyhedrosis virus insect killing suspending agent, in which the virus bearer content is 5-20 hundred million PIB / ml., itis composed of chlorbenzuron analogue chlorfluazuron, aromatic amino acid tyrosine, fluorescent whitener, dispersant, glycerin, sterilized water. The present invention is harmless to man and livestock and other higher animals, has no pollution to environment and has no pesticide residue.

The invention belongs to the field of pharmaceutical formulations specially used for animals, and relates to an in-situ gel formulation for florfenicol injection and a preparation method thereof. The in-situ gel formulation is prepared from antibacterial drugs, an in-situ gel substratum and other indispensable auxiliary materials, wherein the antibacterial drugs are specially used for animals; the in-situ gel substratum is accredited in the field. The preparation method is illustrated.The in-situ gel formulation disclosed by the invention is freely flowing liquid at room temperature, forms the semisolid gel after intramuscular injection or hypodermic injection administration, realizes only one-time administration within one treatment course, has the advantages of easiness for preparation, stability in property, convenience for administration, long administration effect lasting time, definite treatment effect, high animal adaptability, no toxic or side effect or adverse reaction, and the like, achieves the broad-spectrum antibacterial effect and is suitable for preventing and treating various sensitive bacteria infection of the animals, such as pigs, sheep, dogs, cats, cattle, and the like.

The invention belongs to the technical field of animal manure comprehensive utilization and culture, and relates to a method for culturing maggot by utilizing livestock and poultry manure slurry. The method comprises the following steps: collecting animal manure in a temporary animal manure lagoon; fermenting effective microorganism (EM) strain, or enzymatic microorganism, or mixed strains of the EM strain and the enzymatic microorganism to ensure that the manure slurry is liquefied to form liquefied manure slurry; putting putrescent food in an outdoor fly concentrated position; putting collected seed maggot on a net chain above a fly cage to collect fly eggs and maggot; and maintaining the temperature of diluted manure slurry in a maggot culture pond, introducing oxygen to ensure that the maggot is cultured for 3 to 4 days and developed into imagochrysalis, and capturing by utilizing a net bag. The culture process has the advantaages of simple process, high animal manure utilization, safe and reliable equipment, high maggot culture benefit, large comprehensive utilization value, high quality of maggot and light environmental pollution, and can be used for large-scale industrial culture.

The invention discloses a production technology of aspergillus oryzae fermented soybean meal. The production technology comprises the following steps: (1) aspergillus oryzae strain activation; (2) aspergillus oryzae strain liquid culture; (3) aspergillus oryzae strain culture in a triangular flask; and (4) soybean meal fermentation. The production technology is simple and easy to understand, the produced soybean meal is a main protein raw material of animal feeds, and has the advantages of high crude protein content, reasonable amino acid composition, high animal digestion utilization rate and the like. Aspergillus oryzae also can produce amylase, saccharifying enzyme, cellulase, phytase and the like, and under the action of protease, nondigestible macromolecular proteins are degraded into peptone, peptides and various amino acids, and so that crude fiber, phytic acid and other matters, which are difficult to absorb, in accessories can be degraded, and the nutritional value, health function and digestive rate can be improved.

The invention relates to high purity gangliosides preparation method. It includes the following steps: using higher animal brain cell as raw material; adding solvent to homogenate and do de-lamination; processing gnagliosides extracting solution by once new type macroporous resin column to gain the gangliosides which purity is more than 98%. The known gangliosides compound has extensive biological activity. And its main function is to cure and improve impaired neurological disease. The invention adopts new type macroporous resin for the first time, utilize resin structure to remove harmful substance, separate effective constituent, and keep its biological activity. It is fit for industrialization production.

The invention relates to a later-period compound feed for environment-friendly nonresistant broilers. The compound feed has high animal digestion utilization rate, can maintain the flora balance of digestive tracts effectively and is low in cost, and environment pollution is reduced. The later-period compound feed is prepared from the following raw materials in part by weight: 580 to 630 parts ofcorn, 90 to 120 parts of bean pulp, 85 to 105 parts of wheat middlings, 25 to 45 parts of vegetable dregs, 50 to 70 parts of distiller dried grain with soluble (DDGS), 20 to 40 parts of corn albumen powder, 20 to 30 parts of meat bone meal, 3 to 7 parts of calcium carbonate, 0.3 to 0.7 part of composite vitamin, 10 to 30 parts of grease, 2.0 to 6.0 parts of lysine, 0.2 to 0.6 part of methionine, 0.1 to 0.5 part of threonine, 2.0 to 3.0 parts of table salt, 0.3 to 0.7 part of antioxidant, 0.3 to 0.7 part of microecological preparation and 0.1 to 0.5 part of phytase.

The invention relates to a probe for deep brain electromagnetic coupling stimulation and electric signal detection, and belongs to the technical field of infrared / terahertz. The probe comprises a probe tubular structure and a probe end seal. The probe tubular structure is sequentially composed of a probe shell, a polycarbonate insulating sleeve layer, a brass sleeve layer, a polycarbonate base layer, a silver film and a silver iodide film from the outer side to the inner side. The brass sleeve layer extends out of the probe tubular structure to form a probe tip; an optical fiber lens is embedded in the probe tip; and the tip of the probe tip is sealed with cycloolefin resin. The probe provided by the invention not only can couple a high-frequency electromagnetic signal into a specific target in the brain of a higher animal, but also can cling to nerve cells of the target to carry out real-time electric signal detection on the stimulation effect. The deep brain electromagnetic stimulation probe can provide an efficient, minimally invasive and high-targeting stimulation mode and can accurately detect the action potential of a functional area to finally achieve more effective deep brain electromagnetic stimulation and detection.

The invention discloses a method for preventing and curing tobacco mosaic virus disease which includes the following steps of extracting a RNA gene group of the tobacco mosaic virus; utilizing the RNA gene group of the tobacco mosaic virus to acquire a cDNA of movement protein of the tobacco mosaic virus by reverse transcription; utilizing a PCR technology to separate a section of a 21-23bp length in 150-800bp on the cDNA of the movement protein of the tobacco mosaic virus; cloning the section into a plasmid carrier that can express the RNA so as to lead the cloned section to be expressed as the RNA in a colon bacillus of an enzyme lacking the RNA; crushing a cell and making the cell into a suspension solution with the chroma of 2-20ng / ml; spraying the suspension solution on the leaf surface of the tobacco after suspension solution is diluted 1000 times. The method has the advantages of simple and convenient production, low cost and no harm to human, animals and higher animals. The invention is suitable for massive use on tobaccos. The prepared suspension solution overcomes the defects of wettable powder and emulsion; accessory ingredient is easy to disperse. The invention is more convenient to apply in fields and has no remnant and environment pollution.

The invention discloses an instant bean curd dreg food and a processing method thereof. The instant bean curd dreg food is processed from the following raw materials in parts by weight: 10-15 parts ofbean curd dregs, 12-20 parts of chicken, 10-15 parts of wheat flour, 10-15 parts of wheat protein powder, 4-7 parts of wheat bran, 4-7 parts of soybean oil, 0.2-0.4 part of konjac, 0.3-0.6 part of carrageenan, 0.2-0.4 part of Gellan gum, 0.1-0.3 part of glutamine transaminase, 40-50 parts of water, and an appropriate amount of seasonings. During the processing processes, the bean curd dregs and the wheat bran are stir-fried so as to produce aroma, and sieving treatment is then performed; the chicken with extremely high animal protein content and the wheat protein powder which is used as an auxiliary protein are added; and then, enzymatic hydrolysis and protein recombination are performed on the proteins in the mixture by using the glutamine transaminase, and thus, multiple improvements onproduct nutrition, flavor and taste are realized. The final instant bean curd dreg food prepared according to the processing method is high in protein content, low in glycemic index, and rich and comprehensive in nutrients; and thus, the instant bean curd dreg food is especially suitable for patients with diabetes to eat.

The invention discloses a preparation method of an amino acid water-soluble fertilizer containing polyaspartic acid. The method solves the problem of poor prevention and treatment effect of current foliar fertilizer. The method comprises the following steps: 1) collecting and storing the nim leaves, adding a decomposition agent for fully fermenting and treating; 2) adding water in fermented nim leaves, stirring for 1-2 hours and leaching and then centrifuging, collecting a filtrate; 3) adding strong acid in the filtrate and hydrolyzing for 15-22 hours under the temperature of 40-80 DEG C to obtain an amino acid mother liquor; 4) adding trace elements and a conditioning agent in the amino acid mother liquor in a reaction vessel for fully chelating; and 5) detecting and packing to obtain the product. The amino acid water-soluble fertilizer containing polyaspartic acid has a plurality of biological activities such as insecticide and sterilization; has no harm to higher animal, is safe to natural enemy of insects, and has the advantage of no residues on plant.

The invention relates to the technical field of biology and discloses a non-alcoholic chronic fatty liver hepatitis (NASH) rat model and an establishment method and application thereof. An adult rat is fed with special feed (MCD feed) short of methionine and choline or a special high-fat feed (CDAA feed) short of choline, a certain amount of sodium nitrite solution is provided for the animal within a certain time, and the established non-alcoholic chronic fatty liver hepatitis rat model has the characteristics of chronic liver fibrosis and earlier liver fibrosis. The rat model is simple in establishment process, a technical means is easy, the establishment cost is low, the rat model has very high animal model value for scientific research and research and development of drugs, especially serves as a specific animal model with NASH accompanied with severe liver hepatitis and earlier liver fibrosis and belongs to a national initiative.

The present invention discloses a method for preparing an inhibitor for reducing tobacco nicotine synthesis by using a double-stranded RNA interference technology, and applications of the inhibitor. The method comprises: extracting tobacco RNA; carrying out reverse transcription to obtain cDNA of tobacco putrescine N-methyltransferase (PMT) gene; adopting a PCR technology to amplify a fragment in the cDNA of the gene; linking the fragment to a plasmid vector PET-21a capable of expressing RNA; transforming the resulting plasmid into escherichia coli cells with RNAE3 enzyme deletion to express hairpin dsRNA; and breaking the cells to obtain a dsRNA crude preparation, and sequentially adding glycerol, a protection agent and a dispersing agent to the dsRNA crude preparation to obtain the inhibitor for reducing tobacco nicotine synthesis, wherein per strain of the tobacco is applied with 5-15 mL of the prepared inhibitor to reduce tobacco nicotine synthesis. The inhibitor of the present invention has characteristics of convenient preparation, low cost, and no harm on humans and animals, is suitable for a large amount application on tobacco, and has broad industrialization prospects.

The invention belongs to the field of feed production and processing and particularly relates to a Chinese herbal medicine fermented feed additive for pigs to resist heat stress. The invention provides a novel Chinese herbal medicine fermented feed additive for pigs to resist heat stress. The Chinese herbal medicine fermented feed additive is prepared from mint leaves, sea-ear shell, lotus leaves, honeysuckle flowers, herba scutellariae barbatae, radix astragali, lily bulb, fructus crataegi, herba lamiophlomis, rhizoma pinelliae, rhizoma curcumae longae, goldthread roots, magnolia bark, spreading hedyotis herb, lucerne, cicada ecdysis, herba cistanche, rhizome of chuanxiong, herba malachii aquatici, cassia twig, magnolia vine fruit, radix aucklandiae and rhizoma alismatis. The Chinese herbal medicine fermented feed additive has quite high animal disease resistance, has the effects of promoting animal growth, improving the survival rate and reducing the diarrhoea rate, and thus has quite obvious popularization value.

To clarify histamine receptor H3 protein function in vivo, the present inventors constructed a nonhuman higher animal in which the expression of a histamine receptor H3 gene was artificially inhibited. As a result, the present inventors found that this nonhuman higher animal showed increased body weight, food intake, blood insulin level, or blood leptin level compared with a control. Thus, the present inventors found that abnormalities in the histamine receptor H3 protein relate to diseases characterized by changes in body weight or food intake, and this has made it possible to screen drugs for treatment or prevention of these diseases, and to examine these diseases.

A novel peptide found as gonadotropin-releasing hormone (GnRH) in octopuses, a member of mollusks. The structure and the activities of the peptide have been unraveled. The peptide finds use as a reagent used in the studies of correlations between the structure and the activities of GnRH and the information processing mechanisms of the nervous systems in higher animals. It can also serve as a base compound in the development of pesticides and drugs. The peptide has the GnRH activity and has the following amino acid sequence (I): <Glu-Zaa-Zaa-His-Zaa-Ser-Zaa-Zaa-Zaa-Zaa-Pro-Gly-NH2   (I) wherein <Glu represents pyroglutamic acid and Zaa represents any amino acid. Specifically, the peptide has the following amino acid sequence (I): <Glu-Asn-Tyr-His-Phe-Ser-Asn-Gly-Trp-His-Pro-Gly-NH2   (1) wherein <Glu represents pyroglutamic acid.

The invention relates to a biological gene clone expression technology, and particularly relates to a method of rapidly and efficiently constructing an expression vector of a transcription activator-like effector nuclease (TALEN). The method is particularly suitable for constructing an expression vector aiming at higher animal genome genetic modification in large scale so as to study functions of animal genes.

The invention discloses a virus disinsection suspending agent, comprising the azadirachtin, wherein; the main component of the preparation formulation is azadirachtin the, content of which is 0.1% to 10wt%; the virus is a virus or a plurality of viruses of nuclear polyhedrosis virus (NPV); the virus inclusion body content in the suspending agent is 0.5 billion to 20 billion PIB / ml. The invention has the advantages of no harm to human, livestock and other higher animals, no environmental pollution, no pesticide residue, effective control on the occurrence and the damage of the agricultural and forestry pests, stable performance and increased disinsection speed of the products due to the antifreeze, the stabilizing agent, the sun-screening agent, the potentiating agent, the dispersing agent, the defoamer, the pH regulator and other addition agents which are added in the preparation, obvious improvement in disinsection speed and disinsection effect of the virus pesticide.

The invention relates to a research on the prediction of artery pressure baroreflex sensitivity, BRS on dead brain, and the research of antihypertensive ketone selin on dead brain. Wherein, (1), BRS can be used as one independent factor to predict the occur of dead brain, that the occur time of BRS-high animal is later then the lower one; (2), ketone selin can effective improve BRS, without affected by its amount, to delay the dead brain and prolong life time.

The invention relates to a streptomyces polysaccharide degradation bacterium as well as a culture method and application thereof. A streptomyces sp. CB16 strain is preserved in China General Microbiological Culture Collection Center on April 11, 2016 and the preservation number is CGMCC No.12351. The invention further relates to the culture method and the application of the strain. The strain provided by the invention is a multifunctional polysaccharide degradation bacterium; a compound type polysaccharide degradation enzyme preparation prepared from the strain can be used for degrading plant, seaweed, animal and microorganism polysaccharides and particularly has remarkable degradation activity on hyaluronic acid, chondroitin sulfate A, chondroitin sulfate C and chondroitin sulfate E from connective tissues of higher animals; and the streptomyces polysaccharide degradation bacterium has a potential application value and has functions which are completely different from functions of existing streptomyces sp.

The invention provides a double digital PCR (polymerase chain reaction) method of African struthio camelus derived ingredient quantitative detection. A two-channel detection method is used; a digitalPCR system is used; meanwhile, two kinds of fluorescence signals of African struthio camelus specific species gene and higher animal specific gene are detected; the sequence detection probes of the African struthio camelus specific species gene and the higher animal specific gene are respectively marked as FAM and VIC; the sequence copy number of the African struthio camelus specific species geneand the higher animal specific gene is measured through the same PCR system; the relative content of the African struthio camelus derived ingredients accounting for higher animal derived ingredients is obtained through calculation. The method has the advantage that the relative quantification on the copy number proportion of the African struthio camelus derived ingredients accounting for the totalmeat derived ingredients in meat food can be realized.

The invention relates to the technical field of medicines and particularly discloses a compound florfenicol preparation for aquatic animals and a preparation method of the compound florfenicol preparation for the aquatic animals. The compound florfenicol preparation for the aquatic animals is prepared from, by mass, 10-20% of florfenicol, 5-15% of acetylisovaleryltylosin tartrate, 10-20% of glucurolactone, 12-20% of 2-deoxyglucose and the balance auxiliary materials. The preparation method includes manufacturing steps: drying the auxiliary materials until moisture content is 8% or below, respectively subjecting auxiliary and raw materials to grinding and screening through a 80-mesh screen, and well mixing the screened powdery auxiliary and raw materials in a mixer. The compound florfenicolpreparation for aquatic animals is easy to produce, effective in treatment of bacterial diseases of the aquatic animals, low in drug resistance and effective in protection and repair livers of the aquatic animals. By formula and process optimization, problems of poor treatment effects, high animal liver burden and the like of antibacterial medicines are solved. The compound florfenicol preparation is suitable for aquatic animals and used for treatment of various bacterial diseases.

Disclosed herein are methods to assess the biological safety of an agent. The method involves contacting one or more test agents to a culture of C. elegans and analyzing the culture for meiotic disruption, wherein an increase in meiotic disruption of the C. elegans, indicates that the test agent(s) has reduced biological safety to mammals. An increase in meiotic disruption of the C. elegans also indicates a likelihood that the test agent is a reproductive toxicant in higher animals, such as humans. Also disclosed are methods to identifying disruptors of fat homeostasis in a mammal. The method involves contacting one or more test agents to a culture of C. elegans and analyzing the culture for fat content, wherein a change in the fat content, as compared to an appropriate control, indicates that the test agent(s) is a likely disruptor of mammalian fat homeostasis.

The invention discloses a production technology of saccharomyces cerevisiae fermented soybean meal. The production technology comprises the following steps: (1) saccharomyces cerevisiae strain activation; (2) saccharomyces cerevisiae strain liquid culture; (3) saccharomyces cerevisiae strain triangular flask culture; and (4) soybean meal fermentation. The production technology is simple and easy to understand, and the produced soybean meal has the advantages of high crude protein content, reasonable amino acid composition, high animal digestion utilization rate and the like, saccharomyces cerevisiae used in livestock production can save costs, reduce morbidity, and improve the economic benefit, and is helpful to obtain high-quality livestock products.

The invention provides a dual digital PCR (Polymerase Chain Reaction) method for quantitatively detecting turkey derived ingredients. The dual digital PCR method comprises the following steps: simultaneously detecting two fluorescence signals of a turkey specific species gene and a higher animal specific gene by adopting a dual-channel detection method and utilizing a digital PCR system, respectively marking probes for sequence detection of the turkey specific species gene and the higher animal specific gene as FAM and VIC, and calculating relative content of the turkey derived ingredients inhigh animal derived ingredients through copy numbers, which are detected in the same PCR system, of sequences of the turkey specific species gene and the higher animal specific gene. According to thedual digital PCR method provided by the invention, a ratio of the copy number of the turkey derived ingredients in total meat derived ingredients of meat food can be relatively quantified.

The invention relates to bis-biguanide, a preparation method thereof and an application of the bis-biguanide. The bis-biguanide has the advantages that a novel anti-irradiated injury series compound is designed and synthesized, the novel compound which is high-efficiency and low-toxicity is found out and can be used for anti-irradiated injury in the field of civil use and military, by the novel compound, hematopoietic function injury caused by radiation or chemotherapy drugs is reduced, and blood cellular level is increased, or tumor auxiliary treatment is implemented; and a molecular level and animal pharmacological activity test is performed by the compound, and a result shows that the bis-biguanide has high in-vitro activity and high animal treatment effect generally.

Belonging to the feed processing field, the invention relates to insect rainbow trout feed, especially a preparation method of rainbow trout feed with insects as raw materials. The feed is characterized by comprising, by weight: 15-20% of Tenebrio molitor, 15-20% of termites, 15-20% of fly maggots, 15-20% of silkworm chrysalises, 15-20% of eggs, 3-6.5% of prosomillet, 2-5% of alga powder, 4-8% of soybean oil, 2-3% of white radishes, 2-8% of radish seeds, and 2-8% of garlic. The feed of the invention has the advantages of high animal protein content, comprehensive nutrition necessary for promoting rainbow trout growth, strong feeding attraction, high digestibility, gut immunity improvement, virus and disease prevention, good stability in water, difficult pollution to aquaculture water quality, no peculiar smell, no organic residual, better application security, and broader application prospects.

The invention provides a plant for controlling damage of fall webworms and a simple environmental application method of the plant to urban greening trees. The simple environmental application method of the plant to urban greening trees is characterized by including: cutting harvested whole plants of Artemisia lavandulaefolia into 2-3cm long pieces, placing into a vessel, adding 1-2% of edible sugared water, and mixing well; and when Artemisia lavandulaefolia leave the trees to overwinter each year, mixing and grinding the Artemisia lavandulaefolia with the edible sugared water according to a ratio of 2:1, scattering mixed Artemisia lavandulaefolia at the position of 20-30cm around each shade tree, covering all of Artemisia lavandulaefolia with earth once every 5-7 days, and continuously covering for two to three times. By the method, pupation rate of fall webworms is lowered by 60-75%. The plant and the method have the advantages that the natural resource Artemisia lavandulaefolia of China is fully utilized, the plants of Artemisia lavandulaefolia grow massively around China and appear everywhere, obtaining Artemisia lavandulaefolia is simple and easy, and accordingly waste can be turned into wealth; and Artemisia lavandulaefolia is nontoxic to higher animals and beneficial lives, the application method of Artemisia lavandulaefolia to urban greening trees is simple and easy, the cost of the method is about one twentieth of that of the existing methods, evident economic benefit is achieved, and great ecological benefit and social benefit can also be achieved.