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Non-alcoholic chronic fatty liver hepatitis rat model and establishment method and application thereof

A steatohepatitis, non-alcoholic technology, applied in application, pharmaceutical formula, inorganic active ingredients, etc., can solve the violation of welfare principles, cannot meet the research and treatment of NASH, and fails to reflect the pathology of progressive liver fibrosis and liver cirrhosis process and other issues, to achieve the effect of high etiology and pathology research value

Active Publication Date: 2018-03-16
凯斯艾生物科技(苏州)有限公司
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Problems solved by technology

However, the existing MCD diet-induced NASH animal models in the prior art have a mild degree of liver fibrosis, and fail to reflect the pathological process of progressive liver fibrosis or liver cirrhosis
Since NASH is accompanied by liver fibrosis and liver cirrhosis are the core points of the research and treatment of the disease, the animal models under the existing technology cannot well simulate the pathological characteristics of the disease in actual clinical practice, and cannot meet the requirements of NASH research and treatment
At the same time, the mouse models induced by MCD under the existing technology all have serious animal weight loss, and the body weight loss reaches or approaches 40% within the test period, which seriously violates the principle of experimental animal welfare, and the animal model has great defects

Method used

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  • Non-alcoholic chronic fatty liver hepatitis rat model and establishment method and application thereof
  • Non-alcoholic chronic fatty liver hepatitis rat model and establishment method and application thereof
  • Non-alcoholic chronic fatty liver hepatitis rat model and establishment method and application thereof

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Embodiment

[0027] Example: Taking SD male rats as an example, an animal model of non-alcoholic steatohepatitis (NASH) accompanied by chronic liver fibrosis and early liver cirrhosis was constructed

[0028] In this experiment, 35 male SD rats aged 7-8 weeks with a body weight of 220-240 grams were selected. 12 h light and night cycle, temperature 22 ℃ ± 2 ℃, humidity 50%-60%.

[0029] MCD formula: (L-amino acid 175.7g / kg, sucrose 441.9g / kg, corn starch 150.0 g / kg, dextromaltose 50.0g / kg, cellulose 30.0g / kg, corn oil 100.0g / kg, sodium bicarbonate 7.4 g / kg, salt mixture 35.0g / kg, vitamin mixture 10.0g / kg), methionine-choline sufficient control diet MCS formula is MCD diet formula, plus choline 2g / kg, methionine 3g / kg. MCD and MCS The feeds were all processed and produced by Jiangsu Nantong Trofe Feed Co., Ltd., which were clean grade feeds and stored at a low temperature of 4 °C.

[0030] 35 rats were randomly divided into 7 groups after being adaptively fed with common feed for 1 week, ...

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Abstract

The invention relates to the technical field of biology and discloses a non-alcoholic chronic fatty liver hepatitis (NASH) rat model and an establishment method and application thereof. An adult rat is fed with special feed (MCD feed) short of methionine and choline or a special high-fat feed (CDAA feed) short of choline, a certain amount of sodium nitrite solution is provided for the animal within a certain time, and the established non-alcoholic chronic fatty liver hepatitis rat model has the characteristics of chronic liver fibrosis and earlier liver fibrosis. The rat model is simple in establishment process, a technical means is easy, the establishment cost is low, the rat model has very high animal model value for scientific research and research and development of drugs, especially serves as a specific animal model with NASH accompanied with severe liver hepatitis and earlier liver fibrosis and belongs to a national initiative.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rat model of nonalcoholic chronic steatohepatitis and its construction method and application. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is one of the most common clinical liver diseases, among which non-alcoholic steatohepatitis (NASH) is the liver, NAFL) to non-alcoholic fatty liver cirrhosis is a very important link, is one of the important causes of cryptogenic liver cirrhosis. Studies have shown that the prognosis of NASH is poor. About 50% of NASH patients will progress to liver fibrosis after 6 years of follow-up, 10%-15% will develop into liver cirrhosis within 10-20 years, and 9%-26% will develop liver fibrosis after follow-up. within 4-10 years of death due to end-stage liver disease. Therefore, research on the prevention and treatment strategies of NASH is one of the hot spots in the field of liver diseases at home and abroad. [0003] Th...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K20/142A23K20/158A23K20/163A23K20/20A23K50/50A61K33/00A61K49/00
CPCA01K67/02A61K33/00A61K49/0008A23K20/142A23K20/158A23K20/163A23K20/20A23K50/50
Inventor 杨祖经陈继巧涂小蝶庄永杰
Owner 凯斯艾生物科技(苏州)有限公司
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