107 results about "Male rats" patented technology

The invention relates to a hybridization cultivation method for bamboo rats. The method particularly includes the steps that pure wild male rats are used as male parents, second-generation domesticated female rats are used as female parents, and the wild male rats and the female rats are trained gregariously before the oestrum of the female rats and then mate to reproduce high-quality young bamboo rats. By implementing the hybridization cultivation method, the reproduced young rats are strong and big in physique, high in stress resistance, not prone to falling ill and high in survival rate. The reproduced young rats inherit the wild nature of the male parents, rat meat is delicious in taste, strong in flavor and comparable with meat of wild bamboo rats and is rated delicacies of feasts, the bamboo rat products have own features, and benefits of breeders are multiplied.

The invention relates to a composite antioxidant for improving reproductive capacity of a male animal, which belongs to the technical field of animal feed and comprises the following components by weight percent: 6.5-8.5% of food grade vitamin E, 11.0-15.5% of food grade vitamin C, 20.0-40.0% of food grade tea polyphenols, 4.0-6.0% of food grade thioctic acid and the balance of food grade corncobpowder which is taken as a carrier. The composite antioxidant can significantly reduce the content of nitrogen oxide free radicals and malonaldehyde in serum, improve the activity of SOD enzyme, significantly increase the number of sperms and the number of a grade sperms (the sperms with strong activity) in a male rat and improve the reproductive capacity of the male animal.

The invention discloses an establishment method for a type 2 diabetes mellitus animal model. The establishment method comprises the following steps: (1) preparing a lipopolysaccharide solution with aconcentration of 100ug/ml by virtue of normal saline; (2) selecting male rats which are 4-6 weeks old, feeding by virtue of a common feed, and simultaneously injecting the lipopolysaccharide solution;(3) weighing the male rats after 28 days; (4) after the weighing, fasting for 8 hours, separating serum, and measuring blood glucose and insulin; (5) evaluating the resistance of the insulin and glucose stimulated insulin secretion capacity; (6) respectively intraperitoneally injecting an STZ solution into the male rats in the 30th day and the 32th day; and (7) determining that the establishmentof the type 2 diabetes mellitus animal model is finished when the random blood sugar is more than 16.8mmol/L. According to the establishment method, lipopolysaccharide in Escherichia coli 0111:B4 subspecies is selected and is matched with the feeding of the common feed and the STZ injection so as to successfully establish the type 2 diabetes mellitus animal mode; according to the establishment method, the operation is easy, the model establishment cost is low, the model establishment rate is high, and the model establishment period is short.

The invention discloses a model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats, pertaining to fields of medical evaluation and detection technology. The model-making method is characterized in that after healthy Wistar male rats are subjected to electrode-embedding operation, simulation of each rat by clipping tails, overfatigue and operation for drinking and eating disorder can be carried out in order to perform composite model-making operation; by means of weight, electrophysiology activities, gastric emptying, intestine propulsion and objective detection of molecular biology, the purpose of complying a made hepatic stagnation and spleen deficiency type FD model and diagnostic criteria for a clinical hepatic stagnation and spleen deficiency type FD theory is fulfilled in order to achieve consistency of clinical and basic science study; and acquired animals of the hepatic stagnation and spleen deficiency type functional dyspepsia model have symptoms of dyspepsia without other organic pathologic changes.

The invention discloses a method for constructing a model of an animal with chronic cardiac failure heart qi deficiency and blood stasis edema. The method includes the following steps that a clean adult male SD rat is selected, the rat is placed in a cleansing cage, the temperature ranges from 19 DEG C to 23 DEG C, the relative humidity ranges from 40% to 50%, a natural lighting mode is adopted, and the rat is fed through the rat standard feeds and can drink water freely; after adaptive feeding is conducted for one week, lavage is conducted through adriamycin of 0.2 % of the fodder weight, and propylthiouracil injection is conducted on the abdominal cavity one time every week according to the proportion of 3.5 mg/kg and continues for six weeks. The method for constructing the model of the animal with the chronic cardiac failure heart qi deficiency and the blood stasis edema has the advantages that the model can better simulate the chronic cardiac failure heart qi deficiency and the blood stasis edema, meet the pathological characters, for example, LVEF and LVFS reduce remarkably, and BNP increases, of the chronic cardiac failure in western medicine, and show the chronic cardiac failure heart qi deficiency and the blood stasis edema in traditional Chinese medicine, and the copy method is good in stability and repeatability, simple and objective.

The invention discloses a bamboo rat propagation method comprising the following steps of (1) young rat social domestication: breeding young rats in a social breeding pong for 3-4 months; (2) breeding rat selection and separate mating and breeding: selecting male rats and female rats having large propagation potentiality to mate, and putting each pair in a separate culture pond for breeding; and (3) breeding rat mating in the wild: for the breeding rats mated successfully for the first time in the step (2), after young rats are weaned, putting the young rats into the social breeding pond for breeding, and putting the breeding rats into an ecological breeding factory for breeding. By utilization of the social breeding pond, the young rats are adapted to live in groups through domestication, the basis for subsequent large-scale polyculture is established, and injuries and deaths caused by fighting of the bamboo rats during the subsequent polyculture process are reduced and prevented. The wild and interior are combined by the ecological breeding factory designed by the method, the bamboo rats living in the wild natural environment have high survival rates, and are strong and not subject to diseases, mating successful probability of the bamboo rats is improved, and survival rates of the young rats are high.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a medicine for preventing a stroke and treating a stroke sequela, and a preparation method of the medicine. The medicine is prepared by soaking, stir-frying, crushing and mixing raw materials of leech, ginseng, long-noded pit viper, angelica sinensis, ligusticum wallichii, coptis chinensis, notopterygium root, divaricate saposhnikovia root, radix scrophulariae, agastache rugosus, radix angelicae, poria cocos, ephedra, gastrodia elata, yam rhizome, pien tze huang, liquorice, cinnamon, cardamom seed, polygonum multiflorum, amber, radix astragali, rheum officinale, fructus amomi globosi seed, male rat feces, rehmanniae vaporata, manis pentadactyla, scorpio, radix clematidis, radix puerariae, parasitic loranthus, Chinese wild ginger, red peony root, radix linderae, vatica mangachapoi blanco, curcuma zedoary, stiff silkworm, olibanum, myrrh, cinnabar, rhizoma drynariae, rhizoma cyperi, tabasheer, sliced white aconite, crude tortoise plastron, agilawood, fructus caryophylli, arisaema cum bile, carthamus tinctorius, rhinoceros horn, mangnolia officinalis, lumbricus, costus root, ice rind, calculus bovis, resina draconis and ginger. The medicine is good in treatment effect and has no side effect.

The invention discloses a secondary breeding method for bamboo rat culture and relates to the technical field of the bamboo rat culture. The secondary breeding method is characterized by comprising the following steps: (1) separately raising primary breeding male rats and breeding female rats, (2) transferring nest grass parts in feces nests in a room after the breeding female rats are raised independently into a room of secondary breeding male rats, and similarly, transferring nest grass parts in feces nests in the room of the secondary breeding male rats into the room of the breeding female rats, (3) dividing a raising trough for raising the secondary breeding male rats into two independent rooms from the middle side with a separation net, putting the secondary breeding male rats and the breeding female rats in the two rooms respectively for raising for above 3 days, and (4) removing the separation net. According to the method, the successful mating time for secondary breeding is 10-20 days, the time for successful re-pairing is greatly shortened, a success rate of the secondary breeding can reach above 90%, economic benefits are raised, and bite between the male rats and the female rats is avoided.

The invention discloses a Chinese herbal medicine formula for treating nyctalopia and a preparation method thereof. The Chinese herbal medicine formula comprises the following raw materials by weight: 15-20g of atractylodes rhizome, 5-15g of lamb livers, 10-20g of pork livers, 5-15g of male rat gallbladders, 10-20g of carp gallbladders, 15-25g of pale butterflybush flower, 15-25g of sea-ear shell, 15-25g of bat dung, 3-8g of luffa flowers, 15-25g of pine needles, 15-25g of striga asiatica, 10-15g of Japan clover herb, 5-15g of chrysanthemum, 15-25g of phyllanthus urinaria, 5-15g of barbary wolfberry fruit and 5-15g of semen coicis. The Chinese herbal medicine formula has the advantages of omission of surgeries, no side effect, high cure rate and high recovery speed.

The invention discloses a traditional Chinese medicine preparation for treating apoplexia, facial paralysis and hemiplegia, which is prepared from angelica, hemlock parsley, radix astragali, gastrodia tuber, radix sileris, coptis, notopterygium root, figwort, wrinkled gianthyssop, red radix linguae, radix angelicae, tuckahoe, ephedra, moss, curcuma, cinnamon, Amomum Cardamomnm seed, fleece-flower root, amber, rhubarb, tsaoko, male rat manure, radix rehmanniae preparata, pangolin, scorpion, lumbricus, radix clematidis, kudzu root, mistletoe, asarum, red paeonia, radix linderae, green tangerine orange peel, rhizoma atractylodis, stiff silkworm, frankincense, myrrh, cinnabar, rhizoma drynariae, rhizoma cyperi, tabasheer, rhizoma typhonii, tortoise plastron, agilawood, clove, elephantopus scaber, safflower, antler stem, celastrus orbiculatus, lucid ganoderma, rhinoceros horn, Mangnolia officinalis, rosin, costus root, borneol, bezoar, draconis sanguis, tiger bone, Solanum torvum Swartz and Eupatorium chinense. The preparation method is simple and convenient to operate; and the preparation has the advantages of low production cost, high safety and no side effect, and is convenient for patients to take.

The invention discloses an extraction method and an application of a component group of a traditional Chinese medicine extract for treating hemorrhagic stroke. The extraction method comprises the following steps: after crushing rheum officinale, cortex moutan, red peony roots, dried radix rehmanniae and pseudo-ginseng in a certain weight ratio, adding water to decoct and extract; after extracting an extraction liquid by using dichloromethane, extracting a water layer by using n-butyl alcohol; and recovering and drying the n-butyl alcohol extraction liquid to obtain the component group of the traditional Chinese medicine extract. Analyzed by HPLC-MS, the traditional Chinese medicine extract is prepared from 33 components: paeoniflorins, tannins, paeonosides, anthraquinones, stilbenes, saponins and iridoid glycosides and can be used for remarkably reducing levels of neuronspecific enolase of essential hypertension male rats, S-100beta proteins, IL-1, TNF-alpha and plasma fibrinogen and can be used for preparing the medicine for treating hemorrhagic stroke.

The invention relates to a rat culture method for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma and an application of the method. The method and the application thereof effectively solve the problem that stable, reliable and effective model rats are provided for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma, and technical support is provided foranimal experiments and new drug development of pathogenesis and development mechanisms of NASH, liver fibrosis, liver cirrhosis and HCC. The method includes adaptively feeding adult SD male rats weighing 250-300 g for 1 week, feeding a high-fat CDAA feed, adding diethylnitrosamine into drinking water for induction, observing mental state and hair condition of the rats, measuring intake and drinking water, and measuring weight of the rats every week; and calculating from the first feeding of high-fat CDAA diet and the addition of DEN into drinking water, and continuously culturing and feeding for 4-16 weeks to obtain rats transformed from nonalcoholic steatohepatitis to hepatocellular carcinoma. The method is easy to operate, short in time, stable and effective, and provides modeling and technical support for the research and development of new drugs for treating the transformation from nonalcoholic steatohepatitis to hepatocellular carcinoma.

The invention discloses a new function of iridaceous eleutherine plant eleutherine and extracts thereof. The function refers to that eleutherine and extracts thereof have a function of increasing the animal number of female rats captured by normal male rats, the capturing number, the number of ejaculatory animals, and the number of ejaculation times.

The invention relates to a human insulin and analog including rapid-acting insulin (e.g. Insulinaspart, InsulinLispro, and InsulinGlulisine), long-acting insulin (e.g. Insulinglargine, InsulinDetemir, and InsulinDegludec), and modified conjugate (e.g. PEG-Insulin and PEG conjugatedinsulin lispro ), and also relates to the biological value determination method for the cell culture in vitro of PEG-Insulin and application thereof. The external biological activity determination method for human insulin and analog or conjugate is induced in vitro into adipocyte by using preadipocyte of male rat, and measure the glucose consumption after insulin reacting in the adipocyte nutrient solution by using glucose oxidase method to calculate the corresponding insulin biological value.

The invention discloses a kind of ant bearing mouse trap agent, its use and its formula to kill and control the mouse breed. It contains the phosphine oxide aziridine barren medicament, the dait agent and rop findings, the main element, phosphine oxide aziridine can cause gland hurt for female mouse, the sperm occur barrier, at the same time, hae outstanding breed toxicity for female mouse, and can pass through the embryo and latex to transmit to descendant, seriously affect the bearing ability of descendant mouse. The mouse trap can escape the secondary hazarded phenomenon that bring by the traditional mouse trap, at the same time, the mouse cannot appearance the drastic reaction, such as bleed and convulsion after take in the mouse trap solvent, cannot cause awake of mouse, even more appearance the quickly rebound phenomenon of mouse group number after population vacuum, thus, it is a kind of biology ant bearing mouse trap agent that is safe and efficient, can use long period.

The invention discloses an SD rat production method. The first round of mating of female rats and male rats is conducted according to the ratio of 1:2, female rats and male rats are raised separately after female rats get pregnant, and pregnant female rats mate with male rats for the second round according to the ratio of 1:2 1-2 days after giving birth; female rats and male rats are raised separately after female rats get pregnant, and pregnant female rats mate with male rats for the third round according to the ratio of 1:2 1-2 days after giving birth; this is repeated till female rats are replaced. Female rats are fed with 60Co irradiated and sterilized breeding fodder and drink freely. The breeding fodder comprises crude protein, crude fat, crude fiber, crude ash, calcium, phosphorus, N-carbamylglutamate, lysine, methionine, cystine, vitamin, water and the balance glucose. By improving the rotation mating method, adopting the mating mode that the number of male rats is larger than that of female rats, and using the breeding fodder, SD rat reproduction efficiency and newborn rat quality can be improved remarkably.

The invention belongs to the field of biotechnology, and relates to a resource library of rat strains with gene mutation and a preparation method thereof. The rats with gene mutation in the resource library can be obtained from rat spermatogonial stem cells, embryonic stem cells or fertilized ovum with piggyBac transposon insertion mutation. The rats with gene mutation in the resource library also can be obtained from shifting the transposon in germ cells of male rats with mutation. The library can be applied to animal phenotype analysis, gene function research, human disease simulation, medicament screening and drug target development.

The invention discloses a method for researching a lactation promotion effect of oysters. The method comprises the following step of: experimental materials including the oysters, female rats and a male rats. The experiment uses the rats all of which are SPF-graded healthy SD rats; a ratio of the female rats to the male rats is 2:1; both the female rats and the male rats are unbred rats on the ageof 8 weeks; a body weight range of the SD female rats is 180g to 200g; and a body weight range of the SD male rats is 200g to 250g. Main experiment reagents include boric acid, sodium carbonate anhydrous, absolute ethyl alcohol, concentrated sulphuric acid, concentrated hydrochloric acid, potassium sulphate, glucose and n-butyl alcohol. According to the method for researching the lactation promotion effect of the oysters, an overload suckling model is established; the SD rats are used as experiment objects; whether oyster powder has the lactation promotion effect is explored; comparison between lactation yields of the rats in an oyster powder group and a normal group is utilized to illustrate that the oyster powder can improve the lactation yields of the suckling female rats; and comparedto visceral indexes of the female rats in the normal group, visceral indexes of the female rats in the oyster powder group are all higher and accord with the normal healthy physiological level of thesuckling female rats.

The invention discloses a building method for a liver cancer rat model. The method comprises the steps that 1, animals are included, wherein the clean class is Wistar, the number of male rats is 40, the weight is 150+/-20 g, and the male rats are randomly divided into an experimental group including 30 rats and a control group including 10 rats; the animals are reared in a semi-barrier system according to the raising requirement of the clean class of the animals; 2, modeling is performed, wherein sterile tap water is used for preparing a diethylnitrosamine solution with the concentration being100 microgramPml (the solution is kept in a dark place and freshly prepared), the solution is freely drunk by the rats in the experimental group, the solution is changed one time every day, after three months, the solution is replaced with ordinary sterilization tap water, observation continues to be performed for 2 months, and the whole experimental process lasts 5 months; according to the control group, the rats drink the sterilization tap water in the whole experiment process; 3, liver cancer is determined; 4, liver tissue is sampled. A clinic sample is adopted for performing positioning and quantified expression verification, the evidence related clinically is found, the clinic value is evaluated, and the novel clue is provided for liver cancer pathogenesis and liver cancer mechanismresearch. The liver cancer key molecule screened by a question for discussion is adopted for researching a liver cancer marker related to early discovery, classification and evaluation prognosis, andthe research basis is laid for selecting a more effective and accurate liver cancer treatment target position.

The invention discloses a preparation method of a rat metabolic syndrome model and feed used in the method. The preparation method comprises the following steps that 8-week-old Wistar male rats are fed in an environment of 20-22 DEG C and illuminated for 12 hours every day, after adaptive feeding for one week, the rats are fed with the high-fat feed every day, and the rats are continuously fed forless than 18 weeks; the high-fat feed is prepared from, by weight, 60 parts of standard rat feed, 8 parts of egg yolk powder, 12 parts of lard oil, 12 pars of saccharose, 6 parts of peanut powder, 1part of powdered milk, 1 part of cholesterol, 0.01 part of bile salt from pigs, 3 parts of palm oil and 3 parts of coconut oil. The rats are fed with the high-fat feed for a long time, the lipid metabolism disorder and obesity of the Wistar rats are successfully induced, clinical features of metabolic syndromes appear, and the method is an effective method for building the rat metabolic syndrome model.

The invention relates to a method for establishing a rat cerebral hemorrhage model by combining ultrasound with microbubbles, and belongs to the technical field of biological model preparation. The method is implemented by the following steps of 1) taking one SPF-grade male SD rat with the body mass of 240-270 g; 2) making preparation work; 3) with the help of an auxiliary tool, injecting a microbubble contrast agent from the tail vein of the rat, wherein the injection amount is determined according to the body mass of the rat; 4) performing ultrasonic intervention; 5) carrying out CT plain scanning on the head of the rat; 6) irradiating the rat with an incandescent lamp for at least 2 hours, and keeping the anal temperature at 37+/-0.5 DEG C; and 7) scoring the neurological function of the rat, and feeding and observing the rat for later use. According to the method, the contrast agent is ultrasonically targeted, and local blood vessels in an intracerebral target area are destroyed byvirtue of the instantaneous cavitation effect of the microbubbles, so that the rat has an intracerebral parenchymal hemorrhage phenomenon, and the research requirement of spontaneous cerebral hemorrhage is met; the method has the advantages of noninvasiveness, simplicity, convenience and easiness in popularization and is a proper reproducible biological modeling method; and meanwhile, auxiliary equipment is introduced, so that the operation is simpler, more convenient and more feasible.