109 results about "Male rats" patented technology

The invention discloses a bamboo rat breeding method. The method includes the following steps of young rat feeding and hybridization, wherein in the step of young rat feeding, young rats after ablactation are put into a cultivation pond to be fed in a gregarious mode, female rats and male rats are kept together, and after being pregnant, the female rats are separately kept; in the step of hybridization, after the female rats produce young rats and the young rats are ablactated, the female rats are kept separately, each female rat can mate with multiple male rats, and the male rats are replaced once every other two to seven days until the corresponding female rat is pregnant. The young rats are kept in a grouped mode, 15-20 young rats constitute a group, all groups are combined every other fifteen to twenty days, and the young rats are regrouped. According to the bamboo rat breeding method, the bamboo rats can be cultivated to be docile, one female rat can mate with multiple male rats, and accordingly the breeding rate of the bamboo rats is improved; the bamboo rats are kept through fishscale bamboo, so that the physique of the bamboo rats is improved and the breeding rate is improved.

The invention relates to a hybridization cultivation method for bamboo rats. The method particularly includes the steps that pure wild male rats are used as male parents, second-generation domesticated female rats are used as female parents, and the wild male rats and the female rats are trained gregariously before the oestrum of the female rats and then mate to reproduce high-quality young bamboo rats. By implementing the hybridization cultivation method, the reproduced young rats are strong and big in physique, high in stress resistance, not prone to falling ill and high in survival rate. The reproduced young rats inherit the wild nature of the male parents, rat meat is delicious in taste, strong in flavor and comparable with meat of wild bamboo rats and is rated delicacies of feasts, the bamboo rat products have own features, and benefits of breeders are multiplied.

The invention relates to a composite antioxidant for improving reproductive capacity of a male animal, which belongs to the technical field of animal feed and comprises the following components by weight percent: 6.5-8.5% of food grade vitamin E, 11.0-15.5% of food grade vitamin C, 20.0-40.0% of food grade tea polyphenols, 4.0-6.0% of food grade thioctic acid and the balance of food grade corncobpowder which is taken as a carrier. The composite antioxidant can significantly reduce the content of nitrogen oxide free radicals and malonaldehyde in serum, improve the activity of SOD enzyme, significantly increase the number of sperms and the number of a grade sperms (the sperms with strong activity) in a male rat and improve the reproductive capacity of the male animal.

The invention belongs to the fields of medical evaluation, detection technology and experimental zoology, and specifically relates to a construction method of animal model of rat which is used for screening medicaments for treating chronic inflammation, and evaluating methods for treating systematic chronic inflammation. The method comprises the following step that healthy male SD rats are administered with lipopolysaccharide by tail intravenous injection, on an every first day of a week for 4-8 weeks persistently, at a dosage of 120-300 [mu]g per kg of body weight, so that pathologic changes, which meet pathological characteristics of chronic inflammation, mainly of an increased ratio of peripheral blood leucocyte count to neutrophile granulocyte, an increased serum hypersensitive C-creative protein level, infiltration of heart, liver, lung, kidney in inflammatory cells, and haemostasis and oedema of tissues are generated. The method has a good repeatability, and operation of the method is simple, easily controllable and mechanism-clear, besides indexes can be analyzed quantitatively and are convenient for statistics treatments. In addition, the constructed animal model is more ideal and more conform to clinic pathological and physiological changes of the type of disease.

The invention discloses a model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats, pertaining to fields of medical evaluation and detection technology. The model-making method is characterized in that after healthy Wistar male rats are subjected to electrode-embedding operation, simulation of each rat by clipping tails, overfatigue and operation for drinking and eating disorder can be carried out in order to perform composite model-making operation; by means of weight, electrophysiology activities, gastric emptying, intestine propulsion and objective detection of molecular biology, the purpose of complying a made hepatic stagnation and spleen deficiency type FD model and diagnostic criteria for a clinical hepatic stagnation and spleen deficiency type FD theory is fulfilled in order to achieve consistency of clinical and basic science study; and acquired animals of the hepatic stagnation and spleen deficiency type functional dyspepsia model have symptoms of dyspepsia without other organic pathologic changes.

The invention discloses a method for constructing a model of an animal with chronic cardiac failure heart qi deficiency and blood stasis edema. The method includes the following steps that a clean adult male SD rat is selected, the rat is placed in a cleansing cage, the temperature ranges from 19 DEG C to 23 DEG C, the relative humidity ranges from 40% to 50%, a natural lighting mode is adopted, and the rat is fed through the rat standard feeds and can drink water freely; after adaptive feeding is conducted for one week, lavage is conducted through adriamycin of 0.2 % of the fodder weight, and propylthiouracil injection is conducted on the abdominal cavity one time every week according to the proportion of 3.5 mg / kg and continues for six weeks. The method for constructing the model of the animal with the chronic cardiac failure heart qi deficiency and the blood stasis edema has the advantages that the model can better simulate the chronic cardiac failure heart qi deficiency and the blood stasis edema, meet the pathological characters, for example, LVEF and LVFS reduce remarkably, and BNP increases, of the chronic cardiac failure in western medicine, and show the chronic cardiac failure heart qi deficiency and the blood stasis edema in traditional Chinese medicine, and the copy method is good in stability and repeatability, simple and objective.

The invention discloses a bamboo rat propagation method comprising the following steps of (1) young rat social domestication: breeding young rats in a social breeding pong for 3-4 months; (2) breeding rat selection and separate mating and breeding: selecting male rats and female rats having large propagation potentiality to mate, and putting each pair in a separate culture pond for breeding; and (3) breeding rat mating in the wild: for the breeding rats mated successfully for the first time in the step (2), after young rats are weaned, putting the young rats into the social breeding pond for breeding, and putting the breeding rats into an ecological breeding factory for breeding. By utilization of the social breeding pond, the young rats are adapted to live in groups through domestication, the basis for subsequent large-scale polyculture is established, and injuries and deaths caused by fighting of the bamboo rats during the subsequent polyculture process are reduced and prevented. The wild and interior are combined by the ecological breeding factory designed by the method, the bamboo rats living in the wild natural environment have high survival rates, and are strong and not subject to diseases, mating successful probability of the bamboo rats is improved, and survival rates of the young rats are high.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a medicine for preventing a stroke and treating a stroke sequela, and a preparation method of the medicine. The medicine is prepared by soaking, stir-frying, crushing and mixing raw materials of leech, ginseng, long-noded pit viper, angelica sinensis, ligusticum wallichii, coptis chinensis, notopterygium root, divaricate saposhnikovia root, radix scrophulariae, agastache rugosus, radix angelicae, poria cocos, ephedra, gastrodia elata, yam rhizome, pien tze huang, liquorice, cinnamon, cardamom seed, polygonum multiflorum, amber, radix astragali, rheum officinale, fructus amomi globosi seed, male rat feces, rehmanniae vaporata, manis pentadactyla, scorpio, radix clematidis, radix puerariae, parasitic loranthus, Chinese wild ginger, red peony root, radix linderae, vatica mangachapoi blanco, curcuma zedoary, stiff silkworm, olibanum, myrrh, cinnabar, rhizoma drynariae, rhizoma cyperi, tabasheer, sliced white aconite, crude tortoise plastron, agilawood, fructus caryophylli, arisaema cum bile, carthamus tinctorius, rhinoceros horn, mangnolia officinalis, lumbricus, costus root, ice rind, calculus bovis, resina draconis and ginger. The medicine is good in treatment effect and has no side effect.

The invention discloses a secondary breeding method for bamboo rat culture and relates to the technical field of the bamboo rat culture. The secondary breeding method is characterized by comprising the following steps: (1) separately raising primary breeding male rats and breeding female rats, (2) transferring nest grass parts in feces nests in a room after the breeding female rats are raised independently into a room of secondary breeding male rats, and similarly, transferring nest grass parts in feces nests in the room of the secondary breeding male rats into the room of the breeding female rats, (3) dividing a raising trough for raising the secondary breeding male rats into two independent rooms from the middle side with a separation net, putting the secondary breeding male rats and the breeding female rats in the two rooms respectively for raising for above 3 days, and (4) removing the separation net. According to the method, the successful mating time for secondary breeding is 10-20 days, the time for successful re-pairing is greatly shortened, a success rate of the secondary breeding can reach above 90%, economic benefits are raised, and bite between the male rats and the female rats is avoided.

The invention relates to a preparation method of transgenic hamster. According to the following technical scheme, the method comprises the following steps of: enabling an oocyte donation rat and male rat to be normally mated, taking out the fertilized ovum, culturing the fertilized ovum in a special culture solution for the hamster, microinjecting a green fluorescent protein gene, and then transfusing the fertilized ovum to surrogate rat for breeding. The preparation method can be used for overcoming the defect that the transgenic hamster cannot be cultured by virtue of a technology in the prior art.

The invention discloses a Chinese herbal medicine formula for treating nyctalopia and a preparation method thereof. The Chinese herbal medicine formula comprises the following raw materials by weight: 15-20g of atractylodes rhizome, 5-15g of lamb livers, 10-20g of pork livers, 5-15g of male rat gallbladders, 10-20g of carp gallbladders, 15-25g of pale butterflybush flower, 15-25g of sea-ear shell, 15-25g of bat dung, 3-8g of luffa flowers, 15-25g of pine needles, 15-25g of striga asiatica, 10-15g of Japan clover herb, 5-15g of chrysanthemum, 15-25g of phyllanthus urinaria, 5-15g of barbary wolfberry fruit and 5-15g of semen coicis. The Chinese herbal medicine formula has the advantages of omission of surgeries, no side effect, high cure rate and high recovery speed.

An acoustic signal for attracting rats comprising a playback recording or synthetic generation of vocalizations of rats or mice is provided. The vocalizations may be of rat pups, and may be characterized by sonic frequency components in the range of 1.8-7.5 kHz and / or ultrasonic frequency components in the ranges of 21-24 kHz, 40-50 kHz and / or 60-96 kHz. The vocalizations may be of adult male rats, including courting adult male rats, characterized by sonic frequency components in the range of 15-20 kHz and / or ultrasonic frequency components in the range of 20-100 kHz. The vocalizations may be of adult female mice, including nursing adult female mice, characterized by sonic frequency components in the range of 8-12 kHz and / or ultrasonic frequency components in the range of 25-100 kHz.

The invention discloses a traditional Chinese medicine preparation for treating apoplexia, facial paralysis and hemiplegia, which is prepared from angelica, hemlock parsley, radix astragali, gastrodia tuber, radix sileris, coptis, notopterygium root, figwort, wrinkled gianthyssop, red radix linguae, radix angelicae, tuckahoe, ephedra, moss, curcuma, cinnamon, Amomum Cardamomnm seed, fleece-flower root, amber, rhubarb, tsaoko, male rat manure, radix rehmanniae preparata, pangolin, scorpion, lumbricus, radix clematidis, kudzu root, mistletoe, asarum, red paeonia, radix linderae, green tangerine orange peel, rhizoma atractylodis, stiff silkworm, frankincense, myrrh, cinnabar, rhizoma drynariae, rhizoma cyperi, tabasheer, rhizoma typhonii, tortoise plastron, agilawood, clove, elephantopus scaber, safflower, antler stem, celastrus orbiculatus, lucid ganoderma, rhinoceros horn, Mangnolia officinalis, rosin, costus root, borneol, bezoar, draconis sanguis, tiger bone, Solanum torvum Swartz and Eupatorium chinense. The preparation method is simple and convenient to operate; and the preparation has the advantages of low production cost, high safety and no side effect, and is convenient for patients to take.

The invention discloses a bamboo rat breeding method which comprises a first step of selecting a dark and cool environment, and building a bamboo rat nest; a second step of selecting female bamboo rats and male bamboo rats which are of an excellent variety, and placing the female bamboo rats and male bamboo rats in an identical culture tank to perform mixed breeding; a third step of planting crops in a planting tank; a fourth step of feeding the bamboo rats once respectively at 11 am and 8 pm; a fifth step of treating the male rats and female rats extremely poor in gregariousness; a sixth step of applying an estrualization medicine to the female rats at the same period, and applying a medicine to the male rats to improve their sexual desire; a seventh step of performing isolated breeding of pregnant rats; and an eighth step of selecting bamboo rats growing to 2-2.5 kg for slaughter. The bamboo rat breeding method is simple to operate and short in cultivation time, the bamboo rats become full grown and ready for slaughter quickly, diarrhea morbidity and mortality rates are low, the number of laid young rats is high, and farmers' economic benefits can be greatly improved.

The invention discloses an extraction method and an application of a component group of a traditional Chinese medicine extract for treating hemorrhagic stroke. The extraction method comprises the following steps: after crushing rheum officinale, cortex moutan, red peony roots, dried radix rehmanniae and pseudo-ginseng in a certain weight ratio, adding water to decoct and extract; after extracting an extraction liquid by using dichloromethane, extracting a water layer by using n-butyl alcohol; and recovering and drying the n-butyl alcohol extraction liquid to obtain the component group of the traditional Chinese medicine extract. Analyzed by HPLC-MS, the traditional Chinese medicine extract is prepared from 33 components: paeoniflorins, tannins, paeonosides, anthraquinones, stilbenes, saponins and iridoid glycosides and can be used for remarkably reducing levels of neuronspecific enolase of essential hypertension male rats, S-100beta proteins, IL-1, TNF-alpha and plasma fibrinogen and can be used for preparing the medicine for treating hemorrhagic stroke.

The invention relates to a rat culture method for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma and an application of the method. The method and the application thereof effectively solve the problem that stable, reliable and effective model rats are provided for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma, and technical support is provided foranimal experiments and new drug development of pathogenesis and development mechanisms of NASH, liver fibrosis, liver cirrhosis and HCC. The method includes adaptively feeding adult SD male rats weighing 250-300 g for 1 week, feeding a high-fat CDAA feed, adding diethylnitrosamine into drinking water for induction, observing mental state and hair condition of the rats, measuring intake and drinking water, and measuring weight of the rats every week; and calculating from the first feeding of high-fat CDAA diet and the addition of DEN into drinking water, and continuously culturing and feeding for 4-16 weeks to obtain rats transformed from nonalcoholic steatohepatitis to hepatocellular carcinoma. The method is easy to operate, short in time, stable and effective, and provides modeling and technical support for the research and development of new drugs for treating the transformation from nonalcoholic steatohepatitis to hepatocellular carcinoma.

The invention discloses a new function of iridaceous eleutherine plant eleutherine and extracts thereof. The function refers to that eleutherine and extracts thereof have a function of increasing the animal number of female rats captured by normal male rats, the capturing number, the number of ejaculatory animals, and the number of ejaculation times.

The invention relates to the technical field of biological medicine, and specifically discloses an animal model construction method for the study of spleen deficiency and dampness syndrome. The animalmodel construction method comprises two steps that 1, a male rat is selected and fed with high-fat feed, the relative humidity is 53+ / -3%, a dark and quiet environment is maintained, the deep color and light color of the back face of the tongue of the rat are regularly subjected to recording, tongue picture capture, comparative analysis and quantitative grading, physiological and biochemical indexes such as intestinal florae, tryptophan and metabolites of the intestinal florae and tryptophan are measured regularly, physical signs such as hair color, urine and excrement and mental conditions of the rat are recorded regularly, and the construction of the rat model in the depression and damp-heat syndrome stage is completed until 4-6 weeks; 2, the rat in the depression and damp-heat syndromestage continues to be fed with the high-fat feed, the relative humidity is increased to 90+ / -3%, the indexes continue to be detected and observed until 8-10 weeks, and the construction of the rat model in the spleen deficiency and stasis syndrome stage is completed. The rat model construction has the advantages that the model construction method is simple, easy to operate and promote, and close to clinic.

The invention relates to a human insulin and analog including rapid-acting insulin (e.g. Insulinaspart, InsulinLispro, and InsulinGlulisine), long-acting insulin (e.g. Insulinglargine, InsulinDetemir, and InsulinDegludec), and modified conjugate (e.g. PEG-Insulin and PEG conjugatedinsulin lispro ), and also relates to the biological value determination method for the cell culture in vitro of PEG-Insulin and application thereof. The external biological activity determination method for human insulin and analog or conjugate is induced in vitro into adipocyte by using preadipocyte of male rat, and measure the glucose consumption after insulin reacting in the adipocyte nutrient solution by using glucose oxidase method to calculate the corresponding insulin biological value.

The invention discloses a kind of ant bearing mouse trap agent, its use and its formula to kill and control the mouse breed. It contains the phosphine oxide aziridine barren medicament, the dait agent and rop findings, the main element, phosphine oxide aziridine can cause gland hurt for female mouse, the sperm occur barrier, at the same time, hae outstanding breed toxicity for female mouse, and can pass through the embryo and latex to transmit to descendant, seriously affect the bearing ability of descendant mouse. The mouse trap can escape the secondary hazarded phenomenon that bring by the traditional mouse trap, at the same time, the mouse cannot appearance the drastic reaction, such as bleed and convulsion after take in the mouse trap solvent, cannot cause awake of mouse, even more appearance the quickly rebound phenomenon of mouse group number after population vacuum, thus, it is a kind of biology ant bearing mouse trap agent that is safe and efficient, can use long period.

The invention discloses a method of breeding the commercial meat rat of the bamboo rat kind. The method comprises the steps that 1, in the step of group domesticating young rats, the young rats are kept in a group breeding pool for 3-4 months; 2, in the step of rat and meat rat species selection and separate breeding, male rats and female rats with strong and high reproductive potential are selected and paired, each pair of mice are kept separately and fed in a breeding pond, the remaining bamboo rats are kept and fed as meat rat; the feeding uses both crude fiber material and concentrated feed, when the meat rats are fed to reach the selling weight, the meat can be sold. According to the method, the group breeding pool is utilized for young rats to adapt to the group life through domestication, the foundation is laid for the subsequent large-scale polyculture, and casualties caused by bamboo rat fighting in the process of subsequent polyculture are reduced and avoided. The bamboo meat rats having adapted to group living are raised in the natural environment in the wild, the survival rate is high, the body is stronger and the meat rats do not catch illness easily, and the quality of the bamboo meat rat is also greatly improved.

The invention discloses an extraction method and an application of a component group of a traditional Chinese medicine extract for treating hemorrhagic stroke. The extraction method comprises the following steps: after crushing rheum officinale, cortex moutan, red peony roots, dried radix rehmanniae and pseudo-ginseng in a certain weight ratio, adding water to decoct and extract; after extracting an extraction liquid by using dichloromethane, extracting a water layer by using n-butyl alcohol; and recovering and drying the n-butyl alcohol extraction liquid to obtain the component group of the traditional Chinese medicine extract. Analyzed by HPLC-MS, the traditional Chinese medicine extract is prepared from 33 components: paeoniflorins, tannins, paeonosides, anthraquinones, stilbenes, saponins and iridoid glycosides and can be used for remarkably reducing levels of neuronspecific enolase of essential hypertension male rats, S-100beta proteins, IL-1, TNF-alpha and plasma fibrinogen and can be used for preparing the medicine for treating hemorrhagic stroke.

The invention discloses an SD rat production method. The first round of mating of female rats and male rats is conducted according to the ratio of 1:2, female rats and male rats are raised separately after female rats get pregnant, and pregnant female rats mate with male rats for the second round according to the ratio of 1:2 1-2 days after giving birth; female rats and male rats are raised separately after female rats get pregnant, and pregnant female rats mate with male rats for the third round according to the ratio of 1:2 1-2 days after giving birth; this is repeated till female rats are replaced. Female rats are fed with 60Co irradiated and sterilized breeding fodder and drink freely. The breeding fodder comprises crude protein, crude fat, crude fiber, crude ash, calcium, phosphorus, N-carbamylglutamate, lysine, methionine, cystine, vitamin, water and the balance glucose. By improving the rotation mating method, adopting the mating mode that the number of male rats is larger than that of female rats, and using the breeding fodder, SD rat reproduction efficiency and newborn rat quality can be improved remarkably.

The invention provides a method for establishing an SD-rat diabetes model through combination of high-fat diet with induction of STZ. An animal model with type-2 diabetes pathogenesis which is causedby insulin resistance and partial defects of islet B cells is established through clinical characteristics of simulation of human obesity and diabetes by using high-fat feed and STZ induction. SPG-grade SD male rats are divided randomly into normal control groups of 10 rats per group and diabetic groups of 20 rats per group. The rats of 4 weeks old are fed with high-fat diet so that induction of the insulin-resistant model is achieved, and low-dose tail-vein injection of STZ is performed 12 weeks after the rats are fed with the high-fat diet so that establishment of the animal model of diabetes can be induced. Through the method, the clinical characteristics of human type-2 diabetes which is caused by insulin resistance and hyposecretion of insulin can be simulated better, and compared with other modeling methods, the method has more scientific significance of combination of clinic and basic research.

The invention belongs to the field of biotechnology, and relates to a resource library of rat strains with gene mutation and a preparation method thereof. The rats with gene mutation in the resource library can be obtained from rat spermatogonial stem cells, embryonic stem cells or fertilized ovum with piggyBac transposon insertion mutation. The rats with gene mutation in the resource library also can be obtained from shifting the transposon in germ cells of male rats with mutation. The library can be applied to animal phenotype analysis, gene function research, human disease simulation, medicament screening and drug target development.

The invention discloses a method for researching a lactation promotion effect of oysters. The method comprises the following step of: experimental materials including the oysters, female rats and a male rats. The experiment uses the rats all of which are SPF-graded healthy SD rats; a ratio of the female rats to the male rats is 2:1; both the female rats and the male rats are unbred rats on the ageof 8 weeks; a body weight range of the SD female rats is 180g to 200g; and a body weight range of the SD male rats is 200g to 250g. Main experiment reagents include boric acid, sodium carbonate anhydrous, absolute ethyl alcohol, concentrated sulphuric acid, concentrated hydrochloric acid, potassium sulphate, glucose and n-butyl alcohol. According to the method for researching the lactation promotion effect of the oysters, an overload suckling model is established; the SD rats are used as experiment objects; whether oyster powder has the lactation promotion effect is explored; comparison between lactation yields of the rats in an oyster powder group and a normal group is utilized to illustrate that the oyster powder can improve the lactation yields of the suckling female rats; and comparedto visceral indexes of the female rats in the normal group, visceral indexes of the female rats in the oyster powder group are all higher and accord with the normal healthy physiological level of thesuckling female rats.

The invention discloses a building method for a liver cancer rat model. The method comprises the steps that 1, animals are included, wherein the clean class is Wistar, the number of male rats is 40, the weight is 150+ / -20 g, and the male rats are randomly divided into an experimental group including 30 rats and a control group including 10 rats; the animals are reared in a semi-barrier system according to the raising requirement of the clean class of the animals; 2, modeling is performed, wherein sterile tap water is used for preparing a diethylnitrosamine solution with the concentration being100 microgramPml (the solution is kept in a dark place and freshly prepared), the solution is freely drunk by the rats in the experimental group, the solution is changed one time every day, after three months, the solution is replaced with ordinary sterilization tap water, observation continues to be performed for 2 months, and the whole experimental process lasts 5 months; according to the control group, the rats drink the sterilization tap water in the whole experiment process; 3, liver cancer is determined; 4, liver tissue is sampled. A clinic sample is adopted for performing positioning and quantified expression verification, the evidence related clinically is found, the clinic value is evaluated, and the novel clue is provided for liver cancer pathogenesis and liver cancer mechanismresearch. The liver cancer key molecule screened by a question for discussion is adopted for researching a liver cancer marker related to early discovery, classification and evaluation prognosis, andthe research basis is laid for selecting a more effective and accurate liver cancer treatment target position.

The invention discloses a preparation method of a rat metabolic syndrome model and feed used in the method. The preparation method comprises the following steps that 8-week-old Wistar male rats are fed in an environment of 20-22 DEG C and illuminated for 12 hours every day, after adaptive feeding for one week, the rats are fed with the high-fat feed every day, and the rats are continuously fed forless than 18 weeks; the high-fat feed is prepared from, by weight, 60 parts of standard rat feed, 8 parts of egg yolk powder, 12 parts of lard oil, 12 pars of saccharose, 6 parts of peanut powder, 1part of powdered milk, 1 part of cholesterol, 0.01 part of bile salt from pigs, 3 parts of palm oil and 3 parts of coconut oil. The rats are fed with the high-fat feed for a long time, the lipid metabolism disorder and obesity of the Wistar rats are successfully induced, clinical features of metabolic syndromes appear, and the method is an effective method for building the rat metabolic syndrome model.