Method for establishing SD-rat type-2 diabetes model through combination of high-fat diet with induction of STZ

Abstract

The invention provides a method for establishing an SD-rat diabetes model through combination of high-fat diet with induction of STZ. An animal model with type-2 diabetes pathogenesis which is causedby insulin resistance and partial defects of islet B cells is established through clinical characteristics of simulation of human obesity and diabetes by using high-fat feed and STZ induction. SPG-grade SD male rats are divided randomly into normal control groups of 10 rats per group and diabetic groups of 20 rats per group. The rats of 4 weeks old are fed with high-fat diet so that induction of the insulin-resistant model is achieved, and low-dose tail-vein injection of STZ is performed 12 weeks after the rats are fed with the high-fat diet so that establishment of the animal model of diabetes can be induced. Through the method, the clinical characteristics of human type-2 diabetes which is caused by insulin resistance and hyposecretion of insulin can be simulated better, and compared with other modeling methods, the method has more scientific significance of combination of clinic and basic research.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a method for establishing a SD rat type 2 diabetes model induced by a high-fat diet and STZ. Background technique [0002] Diabetes has become a serious public health problem worldwide. At present, about 425 million adults worldwide suffer from diabetes, and type 2 diabetes accounts for 90%-95% of the total number of diabetes. Its huge number of patients also leads to huge medical expenditures . Therefore, establishing an animal model similar to clinical type 2 diabetes is of great significance to medical clinical research. There are many models of diabetes. The current research shows that SD rats, as experimental animals, have similar pathological characteristics of diabetes as humans. In the pathogenesis of type 2 diabetes, two key factors are decreased insulin sensitivity and pancreatic islet B-cell dysfunction. There is evidence that a high-fat diet is one of the independen...

AI Technical Summary

Problems solved by technology

Generally speaking, 30-50% is a high-fat diet, and more than 50% is considered a very high-fat diet. Feeds with high fat content are generally not suitable for molding and storage, so the process requirements are higher and the relative price is more expensive.

The intraperitoneal injection of STZ requires more drug than the tail vein. If STZ is mistakenly injected into the viscera through the abdominal cavity, it is easy to fail the modeling and even cause the death of the rat.

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