A kind of establishment method of type 2 diabetes animal model

Abstract

The invention discloses a method for establishing an animal model of type 2 diabetes. The steps are as follows: (1) prepare lipopolysaccharide solution with physiological saline, and the concentration is 100 μg / ml; Feed and inject lipopolysaccharide solution at the same time; (3) measure the body weight of the male mice after the 28th day; (4) fast the male mice for 8 hours after measuring the body weight, and separate serum to measure blood sugar and insulin; (5) evaluate insulin Resistance ability and evaluation of glucose-stimulated insulin secretion ability; (6) On the 30th day and the 32nd day, the male mice were given intraperitoneal injection of STZ solution once; (7) When the random blood sugar> 16.8mmol / L, the type 2 diabetic animal was determined The model is built. The present invention skillfully selects the lipopolysaccharide in Escherichia coli 0111:B4 subspecies, cooperates with common feed feeding and STZ injection, and successfully establishes the type 2 diabetes animal model. The invention is easy to operate, and the cost of building a model is low, and the molding rate is high and the molding cycle is short.

Description

technical field [0001] The invention relates to the field of animal models of diabetes, in particular to a method for establishing an animal model of type 2 diabetes. Background technique [0002] Diabetes mellitus (DM) is one of the most challenging public health problems in the world. According to the report of the International Diabetes Federation (IDF), there are about 382 million diabetic patients in the world, and it is expected to grow to 592 million by 2035, of which 80% to 85% are type 2 diabetes (type 2diabetes, T2DM). The prevalence of diabetes in China has already ranked first in the world and continues to rise, from 0.67% (1980), 9.7% (2007), to 11.6% (2013). At present, there are 113.9 million adult patients with diabetes in my country, of which type 2 diabetes (T2DM) Accounted for the vast majority. T2DM has a variety of complications (obesity, hypertension, hyperlipidemia), which can easily lead to chronic complications such as coronary heart disease, stroke...

AI Technical Summary

Problems solved by technology

This method takes a long time to induce. Taking male C57BL / 6J mice as an example, after feeding high-fat diet for 4 to 12 months, they showed abnormal glucose tolerance and compensatory increase in blood insulin level; The influence of the dose of STZ single injection, the palatability of high-fat feed is worse than that of ordinary feed, animals often fail to make models due to reduced food intake, and inappropriate STZ single injection dose will prolong the modeling cycle and increase animal mortality

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