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Rapid modeling method for childhood rat type 1 diabetes mellitus

A technology of type 1 diabetes and modeling method, which is applied in the field of rapid modeling of type 1 diabetes in young mice, can solve the problems of easy death of young mice, limitations, low modeling success rate, etc., and achieves high modeling rate and operability. The effect of strong sex and low mortality

Inactive Publication Date: 2018-09-07
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, people have conducted a lot of experimental research on the mechanism and treatment of type 1 diabetes, but the models are limited to adult mice, and the success rate of juvenile mouse type 1 diabetes model is low, and young mice are easy to die. The establishment of T1DM is of great significance to the clinical research and treatment of children with T1DM

Method used

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  • Rapid modeling method for childhood rat type 1 diabetes mellitus
  • Rapid modeling method for childhood rat type 1 diabetes mellitus
  • Rapid modeling method for childhood rat type 1 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Modeling method

[0024] After 3-week-old C57BL / 6 mice were adaptively fed for 3 days, they were marked with ear nails. Fasting and water deprivation for 15 hours before the modeling started, and the fasting body weight and blood sugar were measured and recorded. Randomly divided into blank control group, 30mg·kg -1 group, 40mg·kg -1 group, 50mg·kg -1 group, 60mg·kg -1 groups, 10 mice in each group, and the required dosage of the mice was calculated according to the fasting body weight.

[0025] Dissolve STZ in pH 4.2-4.5 sodium citrate buffer solution (filtered with a 0.22 μm filter in a biological clean bench and stored at 4°C) to prepare a solution with a concentration of 500 ㎎ / ml. The fully dissolved drug solution should be stored away from light, placed on ice, and used up within 30 minutes. It should be prepared and used immediately each time to prevent the drug from being degraded and ineffective. The administration method is as follows: inject...

Embodiment 2

[0026] Example 2 Model Establishes Judgment Criteria

[0027] The mice after 1 week of modeling (that is, the 12th day from the beginning of modeling) were randomly measured for 3 days, and the blood glucose was ≥16.7mmol L for 3 consecutive days -1 The model was successful, and the blood glucose and body weight of mice in each group were measured every week.

[0028] The results showed that observing the general signs of the mice, the mice in each experimental group had the typical diabetes symptoms of polyphagia, polydipsia, and polyuria. Abnormal manifestations of the nervous system such as listlessness or hyperactivity, and its appearance changes such as figure 1 as shown, figure 1 30mg·kg -1 group and 50mg·kg -1 Outline drawings of mice in groups.

[0029] Of which 50mg·kg -1 、60mg·kg -1 The blood glucose of mice in the experimental group was ≥16.7mmol·L for 3 consecutive days -1 , the modeling was successful, and the mice in the 50mg·kg-1 experimental group had t...

Embodiment 3

[0030] Example 3 Detection of pancreatic pathological changes by HE staining and immunohistochemistry

[0031] Pathological specimens of target organs were collected 1 month after modeling. Mice were sacrificed by cervical dislocation, pancreas and kidney were quickly removed, fixed in 4% paraformaldehyde solution, routinely embedded in paraffin, and sectioned. The islet tissue was divided into two parts, one part was stained with HE, and the pathological changes of the islet tissue were observed under a microscope. The other part of the tissue was tested by immunohistochemistry. The islet tissue sections were subjected to xylene gradient dewaxing, ethanol gradient dehydration, distilled water washing, antigen retrieval, antibody incubation and DAB color development, hematoxylin counterstaining, dehydration and transparency, and neutral gum mounting. Microscope observation and photographing.

[0032] 30mg·kg -1 group and 50mg·kg -1 The mice in the group were treated accord...

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Abstract

The invention discloses a rapid modeling method for childhood rat type 1 diabetes mellitus. The method comprises the following steps: performing intravenous injection of streptozotocin for a childhoodrat after abrosia and water deprivation, with injection amount of 50-60mg.kg-1 once, and the frequency of one time each day, which lasts for 4 days continuously; and inducing to establish a model. The invention provides an effective and rapid method for modeling of the childhood rat type 1 diabetes mellitus. According to the method disclosed by the invention, tail intravenous injection is adopted, and in combination with specific STZ dosage, the method is strong in operability and convenient and practical, and a childhood rat T1DM model with typical clinical symptoms can be successfully and rapidly established.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rapid modeling method for juvenile rat type 1 diabetes. Background technique [0002] Type 1 diabetes, formerly known as insulin-dependent diabetes, mostly occurs in children and adolescents, and can also occur in various ages. The onset is relatively sharp, and there is absolutely insufficient insulin in the body, and ketoacidosis is prone to occur. Insulin therapy must be used to obtain satisfactory curative effect, otherwise it will be life-threatening. Type 1 diabetes is an autoimmune disease that threatens human health. Current research results show that type 1 diabetes is mainly mediated by T lymphocytes, attacking and destroying pancreatic β cells, resulting in insufficient insulin secretion, resulting in elevated blood sugar. [0003] At present, 98% of children with diabetes are type 1 diabetes, and the annual incidence rate in my country is 1.04 / 100,000. Epi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7008
Inventor 张毅孟祥宇白博乾刘伟江周娜刘元林李雪王洋王鹏樊月
Owner ACADEMY OF MILITARY MEDICAL SCI
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