67results about How to "High molding rate" patented technology

The invention discloses a coupling compound moulded coal binder and a preparation method thereof. The coupling compound moulded coal binder is a mixture prepared by mixing bentonite, tackifier, sodium carbonate, kaolin and sodium humate; the mixture comprises the following components in parts by weight: 350-600 parts of the bentonite, 45-55 parts of the tackifier, 3-5 parts of the sodium carbonate, 280-320 parts of the kaolin and 100-125 parts of the sodium humate; the tackifier is prepared by smashing 10-15 parts by weight of polyacrylate, 5-10 parts by weight of acrylamide, 3-5 parts by weight of cellulose, 20-25 parts by weight of cassava stalk, 3-5 parts by weight of modified starch and 0.2-0.5 part by weight of cross-linking agent into 50-100 meshes, and mxing the smashed materials. The preparation method comprises the following steps: preparing the tackifier, stocking, pretreating parts of the components, mixing, stirring and the like. The moulded coal binder has favorable waterabsorbtion performance, the moisture of an extruded and shaped coal bar is quickly evaporated and is easy to dry, thus solving the problems of high raw coal moisture, difficult moulded coal production and low shaping rate in rainy days in the south of China.

The invention patent relates to the technical field of animal experiment model construction, in particular to a construction method for a mouse type 2 diabetes mellitus (T2DM) animal experiment model. The method includes the following steps: male Kunmin mice at the age of 7 to 31 days are selected; 50 to 75 mg / Kg (milligrams per kilogram) of streptozotocin is taken by the mice through intraperitoneal injection at a time; after the mice are continuously fed with high-fat high-glucose feed for 4 to 6 weeks upon free eating, the mice of which fasting blood-glucose is above 7.0 millimoles per liter, blood glucose in a 2-hour oral glucose tolerance test is above 11.1 millimoles per liter, cholesterol is above 5.7 millimoles per liter, triglyceride is above 1.7 millimoles per liter, and high-density lipoprotein is below 1.0 millimole per liter at the same time are defined as T2DM animal experiment models. The model can simulate the T2DM characteristics of humans, and adopts multiple testing indicators to accurately define T2DM; due to the light weights of the underage animals, the streptozotocin dosage is small, so that the experiment cost is reduced.

The invention discloses a modeling method of an Sjogren syndrome mouse model. The modeling method comprises the following steps: killing mice, taking out bilateral salivary glands and peeling off capsules and connective tissues; washing with PBS (Poly Butylenes Succinate); adding the PBS according to the amount of adding 0.5ml of the PBS into each salivary gland; shearing the salivary glands into pieces, and uniformly homogenizing and centrifuging in an ice bath; then taking supernatant and quantifying salivary gland antigens by adopting a BCA (Bicinchoninic Acid) protein quantifying method; adjusting the concentration of the salivary gland antigens to be 4mg / ml by utilizing the PBS; adding equal quantity of an FCA (Freund Complete Adjuvant) or an FIA (Freund Incomplete Adjuvant) and diluting the concentration to be 2mg / ml; repeatedly blowing and beating until two liquid phases are dissolved mutually to form an ivory color; randomly grouping C57BL / 6 mice and shaving off furs on the backs of the mice; carrying out intradermal multi-site injection of 2mg / ml mouse salivary gland antigens prepared by the FCA on the back and tails of the mice on the current day and the 7th day, wherein the injection amount is 0.1ml per mouse; injecting equal quantity of the salivary gland antigens prepared by the FIA on the 14th day through the same method; after modeling for about 6 weeks, detecting indexes and screening the successfully modeled mice. The modeling method disclosed by the invention is high in modeling efficiency and short in modeling time.

The invention relates to a preparation method of a hepatocyte apoptosis animal disease model by coinduction of D-galactosamine and lipopolysaccharide, in particular to a simple inducer formula and a simple operation process which can generate a hepatocyte apoptosis rate above 80% and reproduce a stable and reliable hepatocyte apoptosis animal model. The invention also relates to the application of the hepatocyte apoptosis animal model to the research on the pathogenesis of hepatitis and evaluation of novel anti-hepatitis medicaments.

The invention relates to a full-automatic fruit and vegetable facial mask maker, in particular to a device for generating a facial mask by stirring and emulsifying fruit or vegetable juice and collagen. The full-automatic fruit and vegetable facial mask maker comprises a casing, a base, a facial mask forming disc, a juicing device, an emulsifying and stirring device and a driving device, wherein the driving device is arranged in the casing and used for supplying juicing driving force to the juicing device; the juicing device comprises a screw and a juicing chamber, one end of the screw is detachably connected with the driving device, the other end is nested in the juicing chamber, and the juicing chamber comprises a feeding port formed above the device, a juice outlet communicated with the emulsifying and stirring device as well as a slag hole; and the emulsifying and stirring device comprises magnetic levitation stirring and heating components, a controllable switch opening and a liquid level detector; and the facial mask forming disc can be drawn out of the facial mask maker, and a facial mask is taken out and obtained after a facial mask liquid is cooled and formed. The full-automatic fruit and vegetable facial mask maker adopts squeezing type juicing, the juice yield is high, and the juicing device and the emulsifying device adopt detachable structure, so that the devices can be cleaned.

The invention discloses a method for producing a small-dimension aluminum alloy round ingot. The problem that the yield of ingots is low caused by the fact that the surface of the aluminum alloy round ingot which is produced by a prior method has the crack defect is solved. The method comprises steps of taking and adding aluminum ingots, aluminum-manganese alloy, aluminum iron alloy, aluminum chromium alloy, zinc ingots, copper ingots and magnesium ingots to a resistance reverberatory furnace in accordance with the mass ratio of elements of the aluminum alloy round ingot, conducting complete melting and obtaining a molten body; and feeding the molten body to a stewing furnace of the resistance reverberatory furnace, feeding argon for fining, standing and preparing and obtaining the small-dimension aluminum alloy round ingot through a semi-continuous casting method. Different adding time is selected in accordance with properties of different alloy, the problem of full alloying of all elements is solved, the yield of ingots is high and reaches 90%, and the problem that the forming ratio of ingots produced by the prior method is low is solved, and the method is applied to the field of aluminum alloy production.

The invention discloses a stress loading device and system for constructing a stress fracture animal model and a method, and belongs to the technical field of animal experiment models. The device comprises a tissue fixing unit for fixing experimental animal skeletons, a laser displacement sensor is arranged at one side of the tissue fixing unit, and a stress sensor is arranged at the other side of the tissue fixing unit. A linear actuator is arranged at the other end of the stress sensor, and the tissue fixing unit, the stress sensor and the linear actuator are coaxially arranged. A linear guide rail is further arranged below the tissue fixing unit. The invention further discloses a controllable stress fracture animal model construction system based on the stress loading device. The system comprises the stress loading device, a real-time data collecting and processing module and a PC side LabVIEW control program. The device is precise and controllable in loading strength and time, and the success rate of establishment of the model can be effectively increased.

The invention provides a construction method of a type I diabetes animal model. The method comprises the following steps: selecting C57BL / 6 mice, performing first tail vein injection, continuously performing intraperitoneal injection of a streptozotocin reagent for 5 days, performing ambrosia for 12 hours before injection, and allowing the mice to freely drink water. Two days after the injection procedure is completed, the ambrosia is carried out for 4 hours, and a glucometer is used for measuring blood glucose until the blood glucose value is 10 mmol / L or above. The statistical analysis of the blood glucose measurement result of the type I diabetes animal model shows that the C57BL / 6 mouse type I diabetes model constructed by using the streptozotocin is feasible and can be used for researching the diabetes pathogenesis and the anti-hyperglycemia intervention of medicaments and health-care foods. Compared with the prior art, the C57BL / 6 mouse type I diabetes model constructed by the invention has the advantages of high film forming rate, simple operation, no death of animals and good reproducibility.

The invention relates to a juicing device of a full-automatic fruit and vegetable facial mask machine. The juicing device comprises a barrel, a threaded rod and a driving device, and is characterized in that the driving device and the threaded rod are connected in a driving mode, the threaded rod is arranged in the barrel in a sleeved mode, protruding threads are arranged on the surface of the threaded rod, the gap between the threaded rod and the barrel gradually narrows in the juice outlet direction, a residue outlet end is arranged at the other end of the barrel, the barrel comprises a front barrel body and a rear barrel body, a feed pipe communicated with the inner portion of the barrel is arranged on the upper portion of the front barrel body, a conical cover is coaxially arranged in the rear barrel body, a filter screen is arranged on the conical cover, the conical cover shrinks in the juicing direction, and a juice outlet is formed in the portion, below the conical cover, of the rear barrel body. When the juicing device is used for squeezing of the facial mask machine, the squeezing speed is low, the temperature is low, nutritional ingredients in fruits and vegetables are not prone to damage, fiber compositions reserved in the fruits and vegetables are beneficial to facial mask forming, and facial mask forming quality is improved.

The invention belongs to the technical field of biology, and particularly relates to a tumor tissue transport fluid and an application thereof. The tumor tissue transport fluid provided by the invention comprises a plurality of antibiotics and nutritional factors, is definite in components, does not contain serum, can significantly improve the defect of nutrient depletion of previous tissue transport fluids, and avoids activity reduction of tumor samples in storage or transportation to a great extent. The transport liquid provided by the invention is simple to prepare, can effectively improvethe activity of a fresh tumor sample, can well protect the cellular morphology of the tumor sample, can significantly increase the modeling rate of PDX, shortens the modeling time, has great help forestablishment of a PDX tumor model and has a high commercial application value.

The invention discloses a method for constructing a bronchial asthma model of a primate. The method comprises the following steps by taking house dust mites as allergens, carrying out first week intraperitoneal injection administration, second week intraperitoneal injection administration, fourth week subcutaneous injection administration, fifth week atomization administration, seventh week intramuscular injection administration, eighth week atomization administration, twelfth week intramuscular injection administration, fifteenth week atomization administration on primates to cause sensitization, and constructing a primate model of bronchial asthma According to the method, the bronchus asthma model of the cynomolgus monkey conforms to the clinical characteristics of asthma in the aspectsof inflammatory cells, serum inflammatory cytokines, airway obstruction characteristics and morbidity symptoms, and the hypersensitivity of a sensitization original skin test is positive, which indicates that house dust mites are sensitized in monkey bodies after modeling.

The invention discloses a special system for preparing pellets for calcium carbide semi-coke forming. The system comprises a quick lime crushing device, a solid asphalt crushing device, a quick pyrolytic device, a first mixing device, a second mixing device and a pelletizing device. The invention also discloses a method for preparing pellets for calcium carbide semi-coke forming by the special system. The method includes crushing quick lime and solid asphalt respectively in the crushing devices; mixing the crushed quick lime and solid asphalt in the first mixing device to obtain a mixture; evenly mixing semi-coke powder from the quick pyrolytic device with the mixture entering the second mixing device to obtain a mixed material; pelletizing the mixed material in the pelletizing device. The special system and the method have the advantages that the problems of difficulty in forming binder selection, complicated forming technologies, high forming cost and requirements on subsequent drying during calcium carbide semi-coke forming are solved, and the special system and the method are high in technological forming rate, stable in forming operation, high in formed pellet strength and low in forming cost.

The invention belongs to the technical field of medical science, and particularly relates to an establishment method of a bone marrow suppression mouse model. The problems that at present, a bone marrow suppression animal model is low in modeling rate and poor in repeatability are solved. The method comprises the steps that an NOD or SCID mouse is adopted, the abdominal cavity of the mouse is injected with 150 mg / kg, calculated according to the initial body weight, of cyclophosphamide, injection is conducted every other day, injection is conducted three times in total, the observation period is 15 days, and the bone marrow suppression mouse model can be obtained. According to the method for establishing the bone marrow suppression mouse model, operation is easy, the modeling rate is high, the repeatability is good, the method can be used for various experimental animals, a foundation is laid for research on bone marrow suppression experiments, the bone marrow suppression experiments and clinical application research can be more normalized and standardized, objective and accurate verification on drug efficacy and action mechanisms of various drugs for preventing and treating bone marrow suppression is facilitated, further guidance on selection and evaluation of clinical treatment schemes is facilitated, and guidance on pharmacology experiments and development of new drugs for treating bone marrow suppression is facilitated.

The invention discloses a diabetes-coronary heart disease combined animal model building method based on a ZDF rat; the method comprises the following steps: adaptively feeding the ZDF male rat with diabetes for 2 weeks; injecting isoprenaline hydrochloride solution everyday in a hypodermic manner for 10 days, wherein the injection capacity is 1mg.kg-1; detecting serum creatine jubase (CK) and creatine jubase isozyme (CK-MB) activity, observing tissue form changes, and evaluating the model. The method uses the ZDF rat, and combines continuous administration with high fat feeding so as to form the diabetes-coronary heart disease combined animal model; the method is short in period, and high in modeling rate.

The invention provides a method for building a mouse renal anemia animal model. The method comprises the following steps: 1) preparing a 0.4 mg / mL aristolochic acid solution from aristolochic acid with PBS (Phosphate Buffer Saline); 2) injecting the aristolochic acid solution into the enterocoelia of a mouse for 6 weeks, 3 mg / kg once every 3 days, and detecting anemia, renal function index, renalpathology and capability of secretion of hemopoietin; 3) stopping administration for 6 weeks, and observing the previous indexes again. The mouse renal anemia animal model built by the method is stable and has high modeling rate, and the problem that renal anemia has a higher incidence rate among chronic kidney diseases but a mature renal anemia animal model is lacked at present is solved.

The invention relates to a method for manufacturing a blood stagnation erectile dysfunction animal model. The method comprises the following steps that S1, a rat is placed in electric shock equipment and is given with continuous electrophotoluminescence; S2, the rat is placed in noise equipment and given with high-frequency noise stimulation; S3, the rat is placed in swimming equipment with ice and water mixed and made to swim. The blood stagnation ED rat model is successfully built by adopting composite factors of electric shocks, noise and ice swimming, the situations that the stimulation strength is weak, model forming time is prolonged, and model forming efficiency is low are avoided, and meanwhile the problem that adaptation easily occurs due to single weak stimulated animals is solved. In addition, the composite factors of the method are convenient to implement, quantification is easy, currents and the noise can be controlled within a proper range, and the model forming efficiency is high.

The invention discloses a polymethyl methacrylate mold for manufacturing a complex-section tunnel lining model. The polymethyl methacrylate mold comprises a polymethyl methacrylate base, a polymethylmethacrylate outer mold and one or a plurality of polymethyl methacrylate inner molds. The polymethyl methacrylate outer mold is a closed type cylindrical body composed of a plurality of curved wall type columnar outer mold plates with outside handles, and a joint seam is reserved between every two adjacent outer mold plates. Each polymethyl methacrylate inner mold is a closed type cylindrical body composed of a plurality of curved wall type columnar inner mold plates with inside handles, and a joint seam is reserved between every two adjacent inner mold plates. Clips are arranged at the top joint seams of the polymethyl methacrylate outer mold and the polymethyl methacrylate inner molds. The polymethyl methacrylate base is provided with an outer mold slot and one or a plurality of inner mold slots. The bottom of the polymethyl methacrylate outer mold is completely inserted into the outer mold slot, and the bottoms of the polymethyl methacrylate inner molds are all inserted into the inner mold slots. The polymethyl methacrylate inner molds are located on the inner side of the polymethyl methacrylate outer mold, and a tunnel lining model pouring groove is reserved between the polymethyl methacrylate inner molds and the polymethyl methacrylate outer mold.

The invention discloses a machining device and method for a magnesium alloy sacrificial anode bar, and relates to the technical field of machining of magnesium alloy sacrificial anode bars. The machining device comprises a first motor, a three-jaw chuck, a nut and a magnesium alloy sacrificial anode bar body, wherein the three-jaw chuck is arranged at the output end of the first motor. According to the machining device and method for the magnesium alloy sacrificial anode bar, through the design of an auxiliary friction welding device, the device can conveniently complete multi-angle reciprocating hammering in the friction welding machining process of the magnesium alloy sacrificial anode bar, so that the friction welding machining effect and forming stability of the magnesium alloy sacrificial anode bar are further improved; and through the design of an automatic clamping anti-shaking and anti-falling feeding device, the device can conveniently complete automatic positioning of anti-shaking and anti-falling of the magnesium alloy sacrificial anode bar in the machining process of the magnesium alloy sacrificial anode bar, so that machining of the magnesium alloy sacrificial anode bar is facilitated, and the machining forming rate is increased.

The invention discloses a method for establishing a meriones meridianus hepatic alveolar echinococcosis animal model. By adopting a series of technical means, such as screening of experimental animals, dissection and washing of cysts, preparation of protoscoleces suspension, microscopic examination of protoscoleces, anesthesia of meriones meridianus and modeling, the method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model is creatively provided. According to the hepatic alveolar echinococcosis animal model established by means of the method, 60 d cyst tissue almost covers the whole thoracic cavity and completely covers the lung, and 15d, 45d and 60d meriones meridianus cyst coefficient is significantly different from the BALB / c mice cyst coefficient. The provided method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model has the advantages of being high in molding rate, short in molding cycle, low in cost and good in repeatability, and the method in widely used in the medical technical field and also practical for the medical technical field.

The invention discloses an application and construction method of a high-selenium induced insulin resistance animal model. The application and construction method comprises the following steps of: feeding 100mug / kg-200ug / kg of selenium to the stomach of an SD (Sprague Dawley) rat every day; and six weeks later, obtaining the insulin resistance animal model. According to the statistic analysis of glucose tolerance and insulin tolerance, insulin signal transduction and gluconeogenesis key enzyme test, the construction of the insulin resistance animal model by utilizing sodium selenite is feasible; and the insulin resistance animal model can be used as a high-selenium induced insulin resistance animal model and a pathogenesis and drug action mechanism research model of diabetes mellitus II. The insulin resistance rat model constructed by the application and the construction method disclosed by the invention has the advantages of simpleness in modeling method, high model establishing success rate, low cost, short period, good reproducibility and the like.

The invention provides a construction method of a rapid heart failure model of a type 2 diabetes mellitus mouse, and relates to application of AGN193109 in the aspect of construction of the rapid heart failure model of the type 2 diabetes mellitus mouse, in particular to a method for obtaining the type 2 diabetes mellitus mouse model through high fat diet and streptozotocin combined induction and then performing administration of a small molecule compound AGN193109. According to the invention, the heart failure time of the mouse is shortened by half under the condition of not adding drugs which can generate toxic and side effects on the myocardial of the mouse with type 2 diabetes mellitus, so that the purposes that the myocardial structure function is damaged in 15 weeks while the blood glucose of the mouse is stably and rapidly increased and more serious hyperglycemia, hyperlipidemia and insulin resistance are generated are achieved. The model construction method has the advantages of being fast in model forming, high in model forming rate, high in pathological change consistency degree and the like, and the constructed model can be well used as a tool for replication and research of the human type 2 diabetic myocardiopathy and has considerable necessity for related research of the type 2 diabetic myocardiopathy.

The invention provides an automotive chassis protection board and a processing method thereof. The Automotive chassis protection board is produced from, by weight, 30-40 parts of polyvinyl chloride resin, 10-15 parts of polyimide resin, 10-20 parts of glass fiber, 20-30 parts of polypropylene, 15-20 parts of polyethylene glycol terephthalate, 25-30 parts of polymethylsiloxane resin, 15-25 parts of antioxidant, 30-35 parts of dibutyl phthalate, and 0.5-1 part of thermal insulation agent. The automotive chassis protection board has high weather resistance and aging resistance, heat dispersion is good, and machine-shaping rate is high.

The invention discloses an application of imatinib mesylate in preparing drugs used for building congenital cataract animal model. Applying the imatinib mesylate to build the congenital cataract animal model has the advantages of simple method, short period, low cost, high film forming rate, good reproduction quality and the like. The obtained animal model can better simulate the congenital cataract of baby lens caused by receptor signal access barrier of platelet-derived growth factors due to various reasons during the growing process before or after birth, and is applicable to studies on various aspects of the nosogenesis of the congenital cataract, therapeutic drug screening, mechanism of action, and the like.

The invention discloses a rapid modeling method for childhood rat type 1 diabetes mellitus. The method comprises the following steps: performing intravenous injection of streptozotocin for a childhoodrat after abrosia and water deprivation, with injection amount of 50-60mg.kg-1 once, and the frequency of one time each day, which lasts for 4 days continuously; and inducing to establish a model. The invention provides an effective and rapid method for modeling of the childhood rat type 1 diabetes mellitus. According to the method disclosed by the invention, tail intravenous injection is adopted, and in combination with specific STZ dosage, the method is strong in operability and convenient and practical, and a childhood rat T1DM model with typical clinical symptoms can be successfully and rapidly established.

The invention a method for constructing an azoospermia mouse model, comprising the following steps: (1) providing sexually mature male mice weighing 25-35g; (2) dissolving busulfan in DMSO and diluting with normal saline to obtain a diluent; and (3) sterilizing the mouse abdomen with alcohol and drying, absorbing the diluent obtained in step (2) and inserting the needle, and completing the first intraperitoneal injection after withdrawal and confirmation; repeating the injection for three times at intervals in the same day and then obtaining the azoospermia mouse model after 35 to 37 days. Themethod provided by the invention has high success rate of modeling and good stability. According to the invention, modeling of 84 C57BL / 6 mice with azoospermia has been cumulatively completed. Totalnumber of models is 84 / 84 by testicular HE staining identification, and the success rate of modeling is 100%. By the adoption of the method for constructing the model, the death rate of mice is 0%; and according to the method, among 84 cumulatively-injected C57BL / 6 mice, the death toll is 0, and the death rate is 0%.

The invention provides a headgear for preparing an eye disease model, application of the headgear and a preparation method of the eye disease model, and belongs to the technical field of animal model construction. The head tool for preparing the eye disease model comprises a mounting piece, an eye socket frame and an eye disease simulation lens. The mounting piece comprises a fixing part and a mounting part which are connected with each other, and the fixing part is used for fixing an animal skull; the orbit frame is provided with a connecting part and an object viewing through hole, the connecting part is connected with the mounting part, and the object viewing through hole is used for animals to view objects; the eye disease simulation lens is detachably arranged in the visual through hole. The preparation method of the eye disease model is carried out by adopting the headgear for preparing the eye disease model, different types of ametropia can be prepared, the preparation of different types of eye disease models can be reliably realized, and the construction of an animal model is ensured to have relatively good stability consistency and model forming rate.