10results about How to "High molding rate" patented technology

The invention discloses a tree shrew hypertension model making method. The method comprises the following steps: (1) solvent preparation: dissolving palmitic acid in absolute ethyl alcohol to prepare 24mg / ml storage liquid for later use; and (2) diluting the stock solution in the step (1) with 10% BSA (Bovine Serum Albumin) into a solution with the concentration of 6mg / ml, and injecting the solution into the abdominal cavity of the tree shrew according to the dosage of 20mg / kg for 21d to finish modeling. The model preparation method is simple, and the model forming rate is high.

The invention relates to the technical field of biomedicine, and discloses a method for constructing a mouse ovary endometriosis model, which comprises the following steps: identifying endometrial tissues of a same donor mouse and bilateral ovaries of a receptor mouse; constructing an ectopic transplantation model of the receptor mouse, and monitoring the survival states of the receptor mouse in different postoperative stages in real time; the growth condition of the ectopic endometrial tissue on the ovary surface is analyzed, and the tissue structure and pathological change of the ectopic endometrial tissue are recognized; determining a lesion area of the ectopic transplantation model, analyzing pathological characteristics and a formation mechanism of the lesion area, and analyzing a correlation degree between the ectopic transplantation model and human endometriosis; and analyzing the simulation effect of the ectopic transplantation model on human endometriosis, and constructing the ovarian endometriosis model of the receptor mouse based on the modeling effect and the ectopic transplantation model. The model forming rate of the endometriosis model and the accuracy of the research result can be improved.

The application provides a construction system and device of a non-human animal model of tension pneumothorax, and the system comprises: a fixing unit for fixing the non-human animal; a first processing unit for puncturing a puncture needle to the parietal pleura, making a guide wire pass through the needle cavity of the puncture needle to enter the pleural cavity, and withdrawing the puncture needle; a second processing unit for inserting a catheter along the guide wire to make the catheter enter the pleural cavity, pulling out the guide wire, and fixing the catheter; a third processing unit for unidirectional air injection into the pleural cavity through the catheter; and a fourth processing unit for monitoring the range of pneumothorax, determining that the non-human animal model of tension pneumothorax is obtained when the lung sliding of the injection side chest is greater than or equal to 4 / 6 area and lung points are observed, stopping air injection, and obtaining the non-human animal model of tension pneumothorax. The construction success rate of the application is 100%, the construction process is rapid, repeatable and objective in determination, and the application can provide a rapid and repeatable platform for diagnostic research, decompression technology evaluation and training.

The invention discloses a construction method and application of a systemic lupus erythematosus mouse model, the correlation between Rasgrp1 composite heterozygous mutation (p.T214I, p.K322X) and the phenotype of systemic lupus erythematosus of a mouse is found for the first time, and on the basis of the correlation, a novel systemic lupus erythematosus mouse model carrying the Rasgrp1 composite heterozygous mutation (p.T214I, p.K322X) is constructed for the first time. A foundation is laid for research on pathogenesis and intervention strategies of systemic lupus erythematosus, and the application prospect is wide.

The invention belongs to the technical field of medical animal model construction, and particularly relates to a construction method of an animal model for simulating various stages from HPV (human papillomavirus) continuous infection to cervical canceration in a natural infection state and application of the animal model. According to the method, a drug immunosuppressor for promoting HPV infection is combined with MmuPV1 for mouse lower genital tract infection modeling; the constructed animal model is stable and uniform in papilloma virus infection, high in modeling rate, low in fatality rate, short in modeling time, controllable in process and good in reproducibility, can completely reproduce the whole process from HPV continuous infection to cervical canceration, accords with clinical practice, improves the efficiency and quality of model construction, and is suitable for popularization and application. An ideal animal model is provided for follow-up research on an HPV infection carcinogenic mechanism and a virus host interaction mechanism, meanwhile, the model can assist in research, development and screening of clinical treatment methods for preventing and treating cervical lesions at all stages, and clinical transformation, new drug research and development and other work are promoted.

The invention discloses a method for modeling a tree shrew hypertension model, comprising: (1) preparing a solvent: dissolving palmitic acid in anhydrous ethanol to prepare a 24 mg / ml storage solution for standby use; (2) diluting the storage solution in step (1) with 10% BSA to prepare a 6 mg / ml solution, and then administering the solution to the tree shrew by intraperitoneal injection at a dose of 20 mg / kg for 21 days to complete the modeling. The model preparation method is simple and has a high modeling rate.

The present application relates to the biomedical technology field, disclose a kind of dry syndrome intestinal damage mouse model construction method and its application.The construction method includes: (1) preparation contains Freund's complete adjuvant and 1mg / mL mouse intestinal epithelial cell antigen immunization emulsifier I and preparation contains Freund's incomplete adjuvant and 0.5mg / mL mouse intestinal epithelial cell antigen immunization emulsifier II;(2) to mouse injection the immunization emulsifier I or the immunization emulsifier II in batches, obtain the dry syndrome intestinal damage mouse model.The dry syndrome intestinal damage mouse model construction method provided by the present application only immunizes mouse by mouse intestinal epithelial cell antigen and adjuvant, obtains the mouse model that simultaneously shows dry syndrome and intestinal damage.The construction method not only has the advantages of high efficiency, fast, economic, but also is extremely easy to popularize and widely used, has good application value.

The invention relates to the technical field of experimental animal disease models, and discloses an establishment method and application of a damp-heat type eczema animal model. The method comprises the following steps: firstly, enabling animals to eat improperly (the content of high-fat feed is increased from 40wt%-50wt% to 100wt% and is kept unchanged) and to feel damp-heat pathogens (the temperature and the humidity are respectively increased from 24-26 DEG C and 70-80% to 28-32 DEG C and 90-100% and are kept unchanged and baijiu is drenched every other day); and then 0.1-7wt% of dinitrochlorobenzene solution is smeared on the abdomen and the back of the animal for sensitization. The eczema animal model and the traditional Chinese medicine damp-heat syndrome animal model are organically combined together, and the ideal damp-heat type eczema animal model is successfully established. The model has high repeatability, is closer to clinical manifestation of patients with damp-heat type eczema, and is beneficial to pathogenesis research of damp-heat type eczema and research and development and efficacy evaluation of new traditional Chinese medicines.

The invention relates to a method for establishing a sicca syndrome mouse model, which comprises the following steps: injecting an emulsion prepared by mixing thymosin and a Titermax adjuvant into the sole of a mouse to establish the mouse model, and carrying out water intake detection, saliva volume detection, tear volume detection, cornea fluorescein staining detection, histopathology detection and serum immunology detection on the mouse model. And screening to obtain a qualified mouse model. According to the method, construction of the model can be completed only through one-time immunization, secondary damage is small, the experimental period is short, and the model forming rate is high. Meanwhile, comprehensive and reliable verification and evaluation of related characteristic changes are carried out on the model based on the in-vivo and in-vitro aspects, and a new tool and platform are provided for disease research of the sicca syndrome.

The present invention relates to a method for establishing a mouse model of Sjögren's syndrome. The mouse model is established by injecting an emulsion prepared by mixing thymus protein with Titermax adjuvant into the sole of a mouse, and then performing water intake detection, saliva volume detection, tear volume detection, corneal fluorescein staining detection, histopathological detection and serum immunological detection on the mouse model, thereby screening and obtaining a qualified mouse model. This method only requires one immunization to complete the construction of the model, with little secondary damage, a short experimental cycle and a high model success rate. At the same time, the model is comprehensively and reliably verified and evaluated for relevant characteristic changes based on both in vivo and in vitro levels, providing a new tool and platform for the disease research of Sjögren's syndrome.