The invention belongs to the field of coal mining, and particularly relates to an overburden bed separation sectional isolation grouting-and-bashing coal seam mining complete stoping method. The overburden bed separation sectional isolation grouting-and-bashing coal seam mining complete stoping method comprises the following steps that 1, according to geological prospecting data, an overburden keystrata of a stope is determined; 2, the inclination length of a stope face is designed reasonably according to the actual coal seam occurrence range and the reserve volume of a mine, and it is ensured that breaking of the overburden main key strata cannot be caused due to the fact that mining is conducted under the length; 3, a coal pillar with the length of a working face is arranged between twosections in a spaced mode, namely one section is spaced, sectional jump mining is conducted, and the sections on the two sides of the coal pillar are mined firstly; 4, pushing mining is conducted onthe working faces of the two sections, meanwhile, parallel working is conducted, the coal pillar is not reserved, a preset bashing belt is built in a crossheading, close to one side of the coal pillar, of the working face of the coal pillar along the coal wall; and 5, stoping work of the middle section is conducted. According to the overburden bed separation sectional isolation grouting-and-bashing coal seam mining complete stoping method, roadway driving maintenance and the working face working environment are safer and healthier.