8 results about "Hydrophobin" patented technology

The invention relates to the technical field of bioengineering, in particular to a method for promoting high yield of cordyceps militaris sporocarp and application of the method. The invention provides a method for promoting high yield of cordyceps militaris sporocarps, which comprises the following steps of: in a cordyceps militaris mycelium culture stage, spraying hydrophobin CmHYD1 on the surface of a culture medium or mycelium after mycelium is subjected to mycelium stimulation treatment under light. According to the method, hydrophobin CmHYD1 with a specific concentration is exogenously sprayed, so that the growth and development of cordyceps militaris sporocarps can be remarkably promoted, and the yield is effectively increased. Experimental data show that when 400 g of CmHYD1 is sprayed, the number of entities in a single bottle of cordyceps militaris is increased by 231.07% compared with that of a control group, the yield and the biological conversion rate are also synchronously increased by 72.70%, and it is proved that the method can achieve high yield of cordyceps militaris by remarkably increasing the number of the fruiting bodies.

The invention belongs to the technical field of genetic engineering, and discloses application of an anchoring protein INP-N in a PET degrading enzyme system displayed on the surface. According to the invention, after efficient anchoring protein INP-N is screened, FAST-PETase is mediated by the efficient anchoring protein INP-N to construct recombinant escherichia coli of which the surface displays PET degrading enzyme. Furthermore, hydrophobin HFBII is introduced into the surface display system, so that the relative enzyme activity of the PET degrading enzyme is improved by 1.1 times or more. Meanwhile, FAST-PETase and carboxylesterase Est30KL are synchronously expressed, so that the proportion of TPA in a degradation product can reach 95% or above. In addition, the PET degrading enzyme surface display system provided by the invention is excellent in cycle performance, more than 65% of activity can still be retained when PET is cyclically degraded for the third time, and the application cost can be greatly reduced. The method can be applied to the fields of degradation of PET products, preparation of PET degradation agents or BHET degradation agents and the like.

The application discloses a ganoderma lucidum hydrophobin Glhyd694 gene, an encoded protein and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the ganoderma lucidum hydrophobin Glhyd694 gene is shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The ganoderma lucidum hydrophobin has self-assembly characteristics, can perform hydrophobic modification on a material surface, and has excellent emulsification activity and emulsion stability. In the ganoderma lucidum, the protein also participates in tolerance regulation of mycelium to various environmental stresses such as cell wall stress and oxidation stress. The application provides a new gene resource for development of natural surface modified materials and biological protective agents.

A mutant filamentous fungus according to the present invention lacks at least a part of the GAG biosynthesis cluster and at least one hydrophobin gene, and does not biosynthesize α-1,3-glucan.

The invention discloses a method for preparing a high-protein solution from hydrophobin through membrane treatment and ultrasonic assistance, and belongs to the technical field of food protein modification and membrane separation. The preparation method comprises the following steps: dispersing hydrophobic protein in an alkaline solution, adding maltodextrin into the alkaline solution, carrying out multiple times of concentration and dilution operation through membrane treatment to remove salt ions and macromolecular aggregates, and then adjusting the pH value of a solution system to be neutral by adopting an acidic solution; the preparation method comprises the following steps: firstly, preparing a solution, then treating the solution under the ultrasonic action to form a micro-nano particle structure with good colloidal stability, and finally, concentrating and drying to obtain powdery protein with high solubility. The soluble protein powder obtained by the invention can be widely applied to vegetable protein drinks, nutritional formula foods and functional foods.

The invention relates to the technical field of electron microscope sample preparation, and discloses a charge density enhanced hydrophobin HFBI mutant mesh film for a cryoelectron microscope and a preparation method and application thereof. The invention provides a surface charge enhanced HFBI mutant. Targeted enrichment and orientation control on different samples are enhanced by designing charge distribution on an electrostatic interface. According to the hydrophobic protein HFBI mutant carrier network disclosed by the invention, a plurality of key bottlenecks in preparation of a cryoelectron microscope sample are systematically overcome by virtue of a synergistic effect of three aspects of resolution improvement, orientation regulation and enrichment enhancement, and a powerful tool is provided for analyzing a high-resolution three-dimensional structure of biological macromolecules.

The invention discloses a preparation method of a hydrophobin-based bifunctional modified membrane and application of the hydrophobin-based bifunctional modified membrane in the field of artificial lungs, and belongs to the technical field of modification of qi-blood exchange membranes. The modified membrane is prepared by soaking a gas-blood exchange membrane for the ECMO / ECCO2R system in a hydrophobic protein-containing modification liquid for self-assembly modification, the hydrophobic protein is I-class hydrophobic protein, namely EAS protein, and the gas-blood exchange membrane for the ECMO / ECCO2R system is a PMP hollow fiber membrane. The modified membrane shows good CO2 / O2 selectivity in an ECMO / ECCO2R system, has excellent carbon dioxide removal performance and excellent blood compatibility, and is beneficial to improving the separation capacity of membrane lung on CO2 in human blood and the anti-thrombosis capacity.

The invention relates to the technical field of biology, in particular to a hydrophobin HFBII purification method. The invention provides a method for purifying hydrophobin from trichoderma reesei fermentation liquor, which can effectively remove pigments, inorganic salts and impure proteins to obtain high-purity hydrophobin. In specific implementation, solid-liquid separation is realized through multiple times of high-speed centrifugation, and a Trichoderma reesei fermentation broth supernatant containing hydrophobin is collected. Adjusting the pH value, removing residual pigments in the fermentation liquor by using D201 resin and activated carbon, and removing the residual activated carbon through high-speed centrifugation and three-stage filtration to obtain a crude extract; and then, concentrating the crude extract by using a 3kDa membrane, adjusting the pH value, carrying out heating treatment, collecting the supernatant, and carrying out freeze drying to finally obtain the hydrophobin freeze-dried powder. The method is simple to operate, convenient and fast, and has the application prospect of large-scale production of hydrophobin.