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Method for loading bioactive protein on hydrophobic stent material

A technology for bioactive proteins and scaffold materials, applied in the field of preparation of matrix metalloproteinases loaded on hydrophobic scaffold materials, can solve problems such as limited applications, promote ingrowth and tissue regeneration, improve biocompatibility, and increase hydrophilicity sexual effect

Inactive Publication Date: 2017-11-03
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the hydrophobicity of aliphatic polyesters, its further application in the field of biomedical materials is limited, and it is necessary to modify them

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] An electrospinning solution with a concentration of 20% (w / v) was prepared. Dissolve 2.4g of PCL (molecular weight: 80,000) in chloroform / methanol solution to a final volume of 12mL, wherein the volume ratio of chloroform to methanol is 5:1, and stir at room temperature for 24h to fully dissolve the PCL. Inhale the solution into a disposable syringe, and set the parameters of the electrostatic propulsion pump as follows: speed 6.0mL / h, volume 2mL, electrostatic voltage 12kV, use a roller wrapped in aluminum foil to receive, the receiving distance is 15cm, and the collection time is 20min. Finally, the collected electrospun scaffolds were placed in a vacuum drying oven for 48 hours to obtain electrospun porous scaffolds of PCL microfibers.

Embodiment 2

[0014] An electrospinning solution with a concentration of 10% (w / v) was prepared first. Dissolve 1.5g P3HB4HB (4HB contains 12mol% molecular weight 280,000, 3-hydroxybutyrate-4 hydroxybutyrate copolymer, a kind of PHA) in chloroform / methanol solution, the final volume is 15mL, in which chloroform and methanol The volume ratio of P3HB4HB is 3:1, and stirred at room temperature for 24 hours to fully dissolve P3HB4HB. Inhale the solution into a disposable syringe, and set the parameters of the electrostatic propulsion pump as follows: speed 1.0mL / h, volume 2mL, electrostatic voltage 18kV, use a roller wrapped in aluminum foil to receive, the receiving distance is 20cm, and the collection time is 120min. The collected electrospun scaffolds were placed in a vacuum drying oven for 48 hours to obtain electrospun porous scaffolds of P3HB4HB micron fibers.

Embodiment 3

[0016] An electrospinning solution with a concentration of 25% (w / v) was prepared first. Dissolve 3.0 g of PLCL (molecular weight: 300,000) in chloroform / methanol solution to a final volume of 12 mL, wherein the volume ratio of chloroform to methanol is 6:1, and stir at room temperature for 48 h to fully dissolve PLCL. Inhale the solution into a disposable syringe, and set the parameters of the electrostatic propulsion pump as follows: speed 8.0mL / h, volume 2mL, electrostatic voltage 16kV, use a roller wrapped in aluminum foil to receive, the receiving distance is 17cm, and the collection time is 15min. The collected electrospun scaffolds were placed in a vacuum drying oven for 48 hours to obtain electrospun porous scaffolds of PLCL microfibers.

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Abstract

The invention discloses a method for loading a bioactive protein on a hydrophobic stent material. Through the effect of bonding with the hydrophobic end of an amphiphilic protein HFBI, the bioactive protein is loaded onto the degradable aliphatic polyester porous fiber stent material, the bioactive protein is then bonded with the hydrophobic end of a hydrophobin, and thereby a novel bioactive protein-loaded tissue engineering stent material is prepared. The stent material remarkably improves the hydrophilic property of hydrophobic materials, the biocompatibility is good, and the invention proves that the stent material has the effect of promoting cell migration and vascularization. The method can be used for loading a macromolecular protein on the surface of a hydrophobic material, and while the biocompatibility of the material is improved, the activity of the loaded protein is kept, so that the hydrophobic material can play a corresponding biological role.

Description

technical field [0001] The invention relates to a method for loading biologically active protein on a hydrophobic scaffold material, in particular to a preparation method for loading a matrix metalloproteinase on a hydrophobic scaffold material; it also relates to the application of the scaffold material in the field of tissue repair and regeneration. Background technique [0002] Aliphatic polyesters are widely used in medicine, tissue engineering and regenerative medicine due to their good biocompatibility and biodegradability, including polycaprolactone (ε-caprolactone, PCL), polyhydroxyalkanoate (PHA ), polylactic acid (PLA), polylactide-glycolide copolymer (PLGA). However, due to the hydrophobicity of aliphatic polyester, its further application in the field of biomedical materials is limited, and its modification is necessary. [0003] Fungal hydrophobins are a class of amphiphilic proteins with small molecular weight and strong surface activity. Its important proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/18A61L27/22
Inventor 王淑芳姜力唐笛
Owner NANKAI UNIV
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