85 results about "Maldi ms" patented technology

A novel Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) sample support plate is described. The plate comprises a top sample presentation surface and a lower surface, wherein the top sample presentation surface comprises at least one aperture for receiving a sample. The aperture extends through and between the top sample presentation surface and the bottom surface, and contains a porous material that retains and concentrates at the target spot analyte and matrix molecules contained in the sample on the surface of the aperture. Methods for making and using the sample support plate in conventional and automated MALDI-MS are also described.

The invention discloses a silicon-based gold nano bowl array chip, and a preparation method and an application thereof. The silicon-based gold nano bowl array chip comprises a gold nano bowl array and a sulfhydryl modified silicon slice; the gold nano bowl array is spread on the sulfhydryl modified silicon slice, and Au-S bond connection is formed between the gold nano bowl array and the sulfhydryl modified silicon slice. The silicon-based gold nano bowl array chip is applied in MALDI-MS detection of biomolecules. The method for MALDI-MS detection of the biomolecules comprises the following steps: dropwise adding a mixed liquid of a biomolecule sample and a matrix on the silicon-based gold nano bowl array chip, drying and crystallizing, attaching the treated silicon-based gold nano bowl array chip onto an MALDI-MS target plate, and then carrying out MALDI-MS detection. The silicon-based gold nano bowl array chip has the advantages of high sensitivity, good reproducibility, simple operation and low cost.

A method of analyzing an array during and/or after fabrication to obtain information relating to the quality of the array manufacturing process is described. The method includes providing an array of features on a substrate, wherein each feature has one or more polynucleotides bound to the substrate. At least one of the features of the provided array is a cleavable feature. The cleavable feature has one or more polynucleotides bound to the substrate via a cleavable linker. The cleavable feature is then contacted with a matrix material, and a MALDI-MS protocol is used to obtain information about the one or more polynucleotides of the cleavable feature. This information may then be used to evaluate the manufacturing process.

Arginine-containing cysteine-modifying compounds useful for MALDI-MS analysis of proteins are provided. These compounds termed isotope-coded ionization enhancement reagents (ICIER) can provide ionization enhancement in MALDI-MS, relative quantitation, and additional database searching constraints at the same time without any extra sample manipulation. More specifically, ICIER increase the ionization efficiency of cysteine-containing peptides by attachment of a guanidino functional group. ICIER also increase the overall hydrophilicity of these peptides due to the hydrophilic nature of ICIER and thus increase the percentage of recovery of these peptides during sample handling and processing such as in-gel digestion or liquid chromatography. Finally, a combination of both light and heavy ICIER provides an accurate way to obtain relative quantitation of proteins by MALDI-MS and additional database searching constraints (number of cysteine residues in every single peptide peak) to increase the confidence of protein identification by peptide mass mapping.

A method of analyzing a polynucleotide using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The method includes obtaining the polynucleotide bound to a substrate via a linker moiety having a triaryl methyl linker group. The polynucleotide bound to the substrate is then contacted with a matrix material and analyzed by MALDI-MS. During the MALDI-MS analysis, laser radiation is directed at the matrix material, thereby exciting the matrix material and causing cleavage of the linker moiety. Ions generated as a result of this excitation and cleavage process are then analyzed to provide information about the polynucleotide.

The invention discloses application of naphthylethylenediamine inorganic acid salt or naphthylethylenediamine organic acid salt as a matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry). In the application, the MALDI MS is MALDI-TOF MS (matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry); the naphthylethylenediamine inorganic acid salt is naphthylethylenediamine hydrochloride, naphthylethylenediamine nitrate, naphthylethylenediamine phosphate or naphthylethylenediamine sulfate; and the naphthylethylenediamine organic acid salt is naphthylethylenediamine trifluoroacetate, naphthylethylenediamine acetate, naphthylethylenediamine formate, naphthylethylenediamine citrate or naphthylethylenediamine oxalate. The invention technically overcomes the defect that small-molecule samples cannot be effectively analyzed because serious matrix background interference is apt to be caused in a low molecular weight zone when organic small-molecule matrices are commonly used. The matrix used by the invention is not required to be added with ionizing reagent and the requirements on sample treatment are reduced; and since background interference hardly exists within a testing range (m/z: 0-1000), kinds of complex mixed systems can also be analyzed by using the matrix.

Methods are described for detecting and quantifying occult blood in a biological sample using laser desorption mass spectrometry (LD MS). Biological samples that can be analyzed using various embodiments of the present invention include stool (fecal occult blood, FOB), and any bodily fluid including urine, cerebrospinal fluid and other bodily fluids. If the heme or heme metabolite is bound to protein, the sample is treated with acid before analysis to release the porphyrin. Some of the methods use LD MS with a time of flight analyzer (TOF) to detect and measure unbound heme, other hemoglobin metabolites and other molecules that have a porphyrin-based structure, e.g., bilirubin, biliverdin, protoporphyrin IX, and Zinc protoporphyrin in the biological sample. In other methods, matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is used to detect and quantify the individual α- and β-polypeptide chains of hemoglobin.

A MALDI plate suitable for MS or MS-MS analysis provided with a composite coating that comprises a hydrophobic coating and a thin layer coating of a mixture of a MALDI matrix material and an intercalating agent such as a polymer is disclosed. A MALDI plate produced in accordance with the present teachings is useful for suppression of matrix ions in the low mass region (<1,000 daltons) of a MALDI-MS spectrum.

The invention relates to a method to directly take substrate assistant laser MALDI/MS analysis to trace protein and the protein enzymolysis sample desalination enrichment that adopts silicon oxide nanometer particle as adsorbent. It conquers the disturbance from high thickness salt and has good adsorbing effect to protein and protein enzymolysis polypeptide. The method has good desalination function. The silicon oxide has good compatibility with MALDI-MS, so it could take directly analysis and easy to operate, avoid the sample consumption. It could take identification to the protein enzymolysis sample of the thickness 10-10M, and conquers the disturbance from 4M sodium chloride or 8M urea to mass spectrum. The enrichment efficiency could reach 100 times.

Embodiments of a method for demonstrating type and/or source of hair damage comprises extracting protein fragments from a hair sample with an aqueous solution, testing the resulting protein fragments with the MALDI-MS test, and then either comparing the results between a damaged sample and an undamaged sample or comparing the results between a damaged sample and a list of known marker protein fragments to identify the type and/or source of the damage.

The invention discloses application of naphthylhydrazine inorganic acid salt or naphthylhydrazine organic acid salt as a matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry). The MALDI MS is MALDI-TOF MS (matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry); the naphthylhydrazine inorganic acid salt is naphthylhydrazine hydrochloride, naphthylhydrazine nitrate, naphthylhydrazine phosphate or naphthylhydrazine sulfate; and the naphthylhydrazine organic acid salt is naphthylhydrazine trifluoroacetate, naphthylhydrazine acetate, naphthylhydrazine formate, naphthylhydrazine citrate or naphthylhydrazine oxalate; and hydrazine radicals on the naphthylhydrazine inorganic acid salt or naphthylhydrazine organic acid salt are all 1-substituted or 2-substituted. The application technically overcomes the defect that small-molecule samples cannot be effectively analyzed because serious matrix background interference is apt to be caused in a low molecular weight zone when organic small-molecule matrices are commonly used. The matrix used by the invention is not required to be added with ionizing reagent and the requirements on sample treatment are reduced; and since background interference hardly exists when m/z is smaller than 500, various complex mixed systems can also be analyzed by using the matrix.

The invention relates to a matrix material for MALDI, in particular to application of fluoride nanometer materials as a matrix in MALDI-MS. The fluoride nanometer materials are nano-particles with various shapes of ytterbium trifluoride, lanthanum trifluoride, cerium trifluoride, praseodymium trifluoride, neodymium trifluoride, samarium trifluoride, europium trifluoride, gadolinium trifluoride, terbium trifluoride, dysprosium trifluoride, holmium trifluoride, erbium trifluoride, thulium trifluoride, ytterbium trifluoride or lutetium trifluoride, or nano-particles of trifluorides compounded byany two or more of ytterbium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium or lutetium in any proportion. The invention takes rare earth fluoride nanometer materials as the matrix of the MALDI-MS for the first time, develops the matrix of the MALDI-MS with simple and convenient operation and strong universality, and realizes the quick and accurate analysis of the MALDI-MS.

The present invention relates to a probe for the analysis of one or more analytes, particularly proteins or compounds capable of binding or otherwise interacting therewith, by laser desorption/ionisation mass spectrometry, more particularly MALDI MS. It also relates to a protein microarray, a method of producing a protein microarray and a method of analysing a protein microarray. The probe comprises a support having an electroconductive target surface thereon characterized in that the target surface comprises a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. Each discrete target area has an area of less than 1000 μm², more preferably still less than 500 μm², and more preferably still less than 100 μm².