Method for simultanuously enriching desalting and appraising micro protein or polypeptide solution

A trace protein and enrichment technology, applied in the field of biochemical analysis and identification, can solve problems such as limited desalting ability, and achieve the effects of high-efficiency desalination, strong desalination ability and low cost
CN1821777AInactive Publication Date: 2006-08-23FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2006-08-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to a method for synchronously enriching and desalting minim albumens or polypeptide solution and verifying them directly, which takes SiO nm particles as the sorbent to adsorb proteins and their enzyme polypeptides efficiently and overcomes interference of high concentration salt.Since SiO nm particles are compatible with MALDI-MS very well and MALDI-TOF / MS analysis can be done directly to the adsorbed samples without elution to avoid the loss.
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Description

technical field

[0001] The invention belongs to the technical field of biochemical analysis and identification, and specifically relates to a method for synchronously enriching and desalting a trace protein or polypeptide solution and directly identifying it. Background technique

[0002] Matrix-assisted laser desorption ionization / mass spectrometry (MALDI / MS) has become an effective tool in proteomics research due to its fast analysis speed, high sensitivity, and easy operation. However, a large amount of salt or other non-protein components (added during sample pre-treatment) in the analyzed sample will greatly reduce the sensitivity of mass spectrometry, which is called the suppression effect in mass spectrometry research. An effective way to avoid the suppression effect is to desalt the sample. In addition, in the process of proteomics research, a large number of proteins that undertake important life activities of organisms are low-abundance proteins, and their extreme...

Claims

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