56 results about "Ionization mass spectrometry" patented technology

This invention relates to a method for detecting the activity of a toxin produced by bacteria of the families Peptostreptococcaceae or Clostridiaceae using mass spectrometry, which comprises the following steps: a) providing a planar surface suitable for mass spectrometry measurement, with attached affinity molecules; b) incubating the planar surface from step a) with the substrate of the toxin to be detected, to bind the substrate to the affinity molecules; c) subsequently incubating the planar surface from step b) with a sample in which the active toxin is to be detected, in the presence of a modification compound, and incubating it for a time sufficient for the substrate to react with the modification compound in the presence of the active toxin, if present in the sample, to form a modified substrate if the active toxin is present in the sample; d) removing the remnants of the sample; e) subjecting the (modified) substrate on the planar surface to matrix-assisted laser desorption / ionization, ionizing it and subsequently measuring the mass spectrum of the (modified) substrate; f) analyzing the measured mass spectrum, such that if the measured mass spectrum corresponds to the non-modified substrate, the active toxin is not present, and if the measured mass spectrum corresponds to the modified substrate or a mixture of the modified and non-modified substrate, then the active toxin is present in the sample. The invention further relates to a device for the soft landing of affinity molecule ions onto a planar surface suitable for mass spectrometry for carrying out step a) of the above method.

The utility model discloses a Faraday cup of an ion receiver of a thermal ionization mass spectrometer, which comprises a non-metal receiving support, a non-metal receiving electrode and a pair of external fixing metal plates, the non-metal receiving support is concaved inwards to form a notch communicated with the outside, the non-metal receiving electrode is arranged in the notch, and the external fixing metal plates are fixed on the non-metal receiving support. The pair of external fixing metal plates are oppositely fixed on the two side surfaces of the non-metal receiving bracket, and the external fixing metal plates and the non-metal receiving bracket are encircled to enable the notch to only have a state that the top is communicated with the outside, so that the whole Faraday cup retains the rigidity of metal, the outside is not easy to deform, and the service life of the Faraday cup is prolonged. The non-metallic material is adopted in the device, so that the device has the characteristic of not rebounding ions, interference on ion measurement can be effectively reduced, and the measurement accuracy is improved.

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

This provides a method for identifying S. aureus and other bacterial species that make up the coagulase-negative staphylococcus group. [Solution] A method for determining the presence or absence of Staphylococcus species in a species-specific manner, wherein the Staphylococcus species is one or more species selected from the group consisting of S. aureus, S. epidermidis, S. capitis, S. caprae, S. hominis, and S. lugdunensis, and comprises a peak detection step of determining whether one or more peaks selected from a group consisting of peaks corresponding to specific m / z values ​​are detected in a mass spectrum obtained using matrix-assisted laser desorption / ionization mass spectrometry on a test sample with respect to S. aureus, and a bacterial species determination step of determining the presence or absence of S. aureus based on the peak detection result of the peak detection step.

The invention discloses a nano-droplet interface ionization mass spectrum ion source device and an operation method thereof, and relates to the field of nano-droplet interface ionization mass spectrum ion source devices, the device comprises a nano-spray needle used for loading a sample solution and realizing a nano-spray process; the metal electrode is inserted into the nano-spray needle, is in direct contact with the sample solution and is used for providing a high-voltage electric field required by nano-droplet interface ionization for the sample solution; and the ionization chamber is pre-filled with inert auxiliary gas and is used for protecting the interface ionization process of the nano liquid drops. High-sensitivity detection of polycyclic aromatic hydrocarbon in samples such as small-size single cells is realized, and higher signal intensity is obtained; better detection response to weak polar compounds (especially compounds with aromatic skeleton structures) in a mixed sample is realized; the detection of the weak polar compound polycyclic aromatic hydrocarbon in the single cell is realized.

This invention provides a method for detecting dichloronaphthalene, a persistent organic pollutant in soil sediments. The method includes: collecting soil sediment samples, pre-treating them by freeze-drying, grinding, and sieving to obtain a sample; extracting and purifying the sample using an organic solvent to prepare a test solution; analyzing and detecting the sample using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (UPLC-APCI-MS / MS), and calculating the measured value of dichloronaphthalene in the test solution by substituting the obtained peak area and fractionation ratio into a specific formula. This invention fills the gap in my country's technology for detecting dichloronaphthalene-type persistent organic pollutants in soil sediments. This method can effectively eliminate interference from complex matrices, accurately determine trace amounts of six dichloronaphthalene isomers, and has the advantages of high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

A mass spectrometry method according to the present disclosure is a mass spectrometry method in a method for discriminating whether a polyethylene terephthalate fiber contained in a target fiber is a recycled fiber. The mass spectrometry method includes a step of preparing a sample and a step of measuring the sample using matrix-assisted laser desorption / ionization mass spectrometry. The step of preparing the sample has a step of dissolving the target fiber in a first solvent to prepare a solution, a step of adding tetrahydrofuran to the solution to separate the solution into a precipitate and a supernatant, a step of obtaining the supernatant, and a step of preparing a mixed solution containing the supernatant and a matrix on a sample plate.

The invention discloses an application of a benzophenone derivative as a bipolar matrix-assisted laser desorption ionization matrix in mass spectrometry detection. The benzophenone derivative matrix provided by the invention can effectively improve desorption ionization efficiency and reduce background interference, is compatible with a positive ion and negative ion bipolar detection mode, and solves the technical bottleneck of single polarity of a traditional matrix detection mode. The matrix has a wide-spectrum absorption characteristic in an ultraviolet region, can be adapted to laser wavelengths of various matrix-assisted laser desorption ionization mass spectrometers, and has a wide application prospect. Besides, when the matrix is used for lipid compound detection, lipid endogenous metabolite spectrum signals with high sensitivity and high coverage rate can be obtained in positive and negative ion modes, the matrix is expected to be widely used for metabonomics and clinical basic research, and key technical support is provided for high-sensitivity and bipolar imaging of small molecule compounds in complex biological samples.

The invention relates to an application of elemental carbon in laser desorption ionization mass spectrometry detection, and the elemental carbon (EC) is obtained by cleaning and extracting components of organic carbon (OC) by using an incomplete combustion product Soot. The EC is used as a material of a graphite-like structure, is used as a substrate when environmental pollution particles are analyzed by using LDI-MS, and can enhance the detection strength of aromatic compounds in the particles. Furthermore, when the EC is used for analyzing aromatic substances, the EC has a better enhancement effect on substances containing reactive hydrogen (PAH with OC containing odd carbon in the invention, namely PAHodd) and substances with lower conjugacy. According to the device and the method, pollution particulate matters are recycled, detection and analysis of particulate matter components are facilitated, and the detection sensitivity of aromatic substances is improved.

The invention discloses an MOF-derived heterojunction, a preparation method thereof and application of the MOF-derived heterojunction in metabolism detection, and relates to the technical field of heterojunction material preparation. The preparation method comprises the following steps: performing first-stage heat treatment on an MOF precursor to prepare a metal oxide, and reacting the metal oxide with a titanium source to obtain a product; or directly reacting the MOF precursor with a titanium source to obtain a product; and carrying out second-stage heat treatment on the product in an inert atmosphere or air to obtain the MOF-derived heterojunction. The invention relates to application of MOF-derived heterojunction as a matrix in detection of serum metabolic fingerprints by laser desorption / ionization mass spectrometry and application in construction of a disease diagnosis model. The heterojunction material prepared by the invention has the advantages of high ionization efficiency, good photothermal effect and strong biological matrix interference resistance, and can be used as an LDI MS nano matrix to be applied to respiratory disease serum metabolism fingerprint diagnosis and disease diagnosis models so as to distinguish respiratory diseases.

The present invention provides matrices for use in mass spectrometry, particularly matrix-assisted laser desorption / ionization mass spectrometry techniques. Methods of use, including for imaging mass spectrometry, of the presently disclosed matrices are also provided.

1. The name of this design product: Medical direct ionization mass spectrometer. 2. Purpose of the product of this design: Direct ionization mass spectrometry technology is used to ionize the test substance in the test sample, and the concentration of the substance is quickly tested and analyzed through the mass spectrometry detector to provide supporting data for medical diagnosis. 3. The key point of the design of this product lies in the three-dimensional image. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The application provides a lung cancer intelligent screening model construction method and system based on sputum direct mass spectrum data. The method comprises the following steps: S1, obtaining the original mass spectrum data of the sputum sample detected by neutral desorption electrospray extraction ionization mass spectrometry, preprocessing including mass spectrum peak detection, peak alignment and intensity normalization to form an initial feature matrix; S2, extracting a core metabolic fingerprint feature set from the initial feature matrix; S3, constructing and training a heterogeneous integrated diagnosis model; S4, optimizing and verifying the hyperparameters of the heterogeneous integrated diagnosis model, and packaging the optimized heterogeneous integrated diagnosis model and the screening rules of the core metabolic fingerprint feature set into a lung cancer screening deployment module. The application realizes high-robustness, high-precision and high-explainability lung cancer non-invasive intelligent screening.

At least one molecule is ionized and a mass spectrometer mass analyzes an m / z range, producing an m / z mass spectrum. A range of N sequential charge states is received. A copy of the m / z mass spectrum is created for each of the N charge states, producing N m / z spectra. Each spectrum of the N spectra is converted to a neutral mass mass spectrum using a different charge state of the N charge states, producing N neutral mass mass spectra. The N neutral mass mass spectra are aligned by neutral mass. When two or more spectra of the N neutral mass mass spectra corresponding to two or more different and sequential charge states include a neutral mass peak above a predetermined intensity threshold at a neutral mass value within a predetermined neutral mass tolerance, the neutral mass value is identified as a neutral mass of the at least one molecule.

This invention relates to the field of industrial quality control and rapid mass spectrometry analysis technology, and discloses a method, device, and system for rapid in-situ / environmental ionization mass spectrometry screening and split determination of target residues in industrial samples. This method addresses time-sensitive scenarios such as incoming material acceptance, process anomaly location, on-site inspection, and emergency splitting. It tackles the challenges of complex industrial sample matrices, low target analyte content, and the need for enrichment and long cycles for confirmatory detection. The method constructs a closed-loop process: "minimalist sampling / introduction – in-situ / environmental ionization – mass spectrometry acquisition – background reference – candidate ion set – matching and exclusion discrimination – splitting decision output." The method acquires sample and background reference spectra within the same acquisition time window or equivalent time window. Candidate feature ion sets are extracted through background subtraction / difference spectroscopy. Combined with repeatability stability, sample / background contrast thresholds, and isotope / adjunct / accompanying ion consistency rules, reference fingerprint database matching and environmental background / non-target spectral exclusion are performed. The system outputs a screening conclusion of "suspected presence / suspected absence / requires verification" and provides triage suggestions for further confirmation (GC-MS, LC-MS / MS, or tandem mass spectrometry). The system integrates sampling / introduction, ionization, mass spectrometry detection, and data processing decision modules. It is adaptable to gas / liquid / solid samples, covering volatile and transpolar molecular systems, and offers advantages such as high speed, low preparation cost, engineerable deployment, and reduced risk of misjudgment.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The invention discloses an acanthosphere-shaped multi-interface type metal oxide magnetic particle and a preparation method and application thereof.The magnetic particle comprises a core composed of magnetic nanoparticles and a shell layer composed of titanium oxide nanosheet spines arranged in a radial mode, and the magnetic particle has superparamagnetism, high specific surface area and rich active sites; and a large number of trap spaces matched with the exosome scale range, so that the method can be used for efficiently enriching and separating the exosome from the biological fluid. Meanwhile, the magnetic core has an out-phase junction composed of amorphous phase titanium oxide and anatase phase titanium oxide, a heterojunction interface composed of titanium oxide and the magnetic core and an ordered spine microstructure, the synergistic effect of the multi-interface effect and the point effect is fully exerted under laser irradiation, the excellent ultraviolet absorption capacity and electron-hole separation efficiency are shown, and the magnetic core has the good application prospect. The method is used for laser desorption ionization mass spectrometry, efficient in-situ pyrolysis of the exosome is achieved, desorption and ionization of metabolite in a lysis solution are promoted, and a high-resolution mass spectrum of the metabolite of the exosome is obtained.

The invention belongs to the field of chemical analysis and detection, and particularly relates to a research method for real-time analysis of a molecular mechanism of gold nanoparticles against bacteria by nanoESI-MS. The method comprises the following steps: performing mixed culture on the gold nanoparticles and bacteria, performing centrifugation, collecting supernate, taking the supernate as a sample solution, and performing analysis by using nanoESI-MS to obtain spectrogram data; performing orthogonal partial least square discriminant analysis on the spectrogram data, and establishing a metabolic substance and sample category prediction model; screening differential metabolites based on the prediction model and projection variable importance; and constructing a correlation network diagram based on the differential metabolites, and analyzing the antibacterial mechanism of the gold nanoparticles. According to the method, analysis can be carried out without sacrificing the integrity of the sample, so that the defects caused by the use of an extracting agent in the traditional mass spectrometry technology are avoided, and the analysis efficiency and the coverage range are also improved.

The invention belongs to the field of mass spectrum ionization, and particularly relates to a photoinduced Penning ionization mass spectrum method. Photoelectrons generated by a photoelectric effect accelerate in an electric field to obtain enough energy, helium He, neon Ne and argon Ar in ground states are excited to metastable states, and the energy of the metastable states of the neutral atoms is higher than 10.6 eV (He excitation energy is 20.62 eV and 19.82 eV); the excitation energy of Ne is 16.72 eV, and the excitation energy of Ne is 16.62 eV; the excitation energy of Ar is 11.72 eV and 11.55 eV), sample molecules with ionization energy higher than photon energy and incapable of being ionized by traditional photoionization can be ionized through Penning ionization, so that the reduction of mass spectrum resolution, sensitivity and quantitative capacity caused by a space charge effect caused by coulomb repulsion among too strong reagent ions in an ion transmission process is avoided, and the detection sensitivity is improved. And damage of too strong reagent ions to the ion detector. The sensitivity can be improved by about 5 times for sample molecules ionized by traditional photo ionization energy, such as toluene, with ionization energy lower than photon energy.

The application discloses a nanoliter spray ionization mass spectrometry interface device, an analytical instrument and an ionization method, and belongs to the technical field of electrospray mass spectrometry ionization devices. The nanoliter spray ionization mass spectrometry interface device comprises an ion guide tube, a first electrode and a second electrode; the inner wall of the ion guide tube is plated with a conductive film; the ion guide tube has an ion transmission channel in the form of a funnel along the length direction of the tube; the ion transmission channel has a first end and a second end; the diameter of the ion transmission channel gradually decreases from the first end to the second end; the first electrode is connected to the end of the first end; and the second electrode is connected to the end of the second end. The device has a simple structure and is convenient to operate; high stability, high sample utilization and high ion transmission efficiency of the electrospray process under a nanoliter flow rate can be realized; positive and negative switching can be directly and rapidly performed under the condition of normal pressure and opening; and problems, such as unstable spraying caused by bad high pressure and complex parameter optimization process, are avoided.

This invention discloses a mass spectrometry patch for wound infection detection and its application, relating to the field of biomedical device technology. The mass spectrometry patch consists of a contact layer, a collection layer, and a protective layer from bottom to top. The contact layer is a polycarbonate mesh film, the collection layer is a polyvinylidene fluoride nanofiber membrane with high-density gold nanoparticles grown in situ on its surface, and the protective layer is a polyurethane film. This invention provides a wearable mass spectrometry patch integrating sampling and detection, achieving non-invasive sampling and direct mass spectrometry analysis. A single detection using laser desorption / ionization mass spectrometry can obtain a global metabolic fingerprint containing hundreds of metabolites, which simultaneously contains pathogen species-specific signals and host metabolic biomarker signals. By training an AI model to analyze the complex fingerprint, rapid pathogen identification and dynamic monitoring of host responses (such as lactic acid and glucose) can be completed simultaneously within 5 minutes, achieving decoupling of pathogen information from changes in host biomarkers.

The invention belongs to the field of environmental sample analysis and detection, and particularly relates to a target-oriented synthesized TCBPA molecularly imprinted polymer, a preparation method thereof and a tetrachlorobisphenol A. After glass fibers are cleaned, the surface of the glass fibers is bonded with the TCBPA molecularly imprinted polymer, and an enrichment probe is obtained; the method comprises the following steps: placing an enrichment probe in a to-be-detected sample solution, carrying out vortex adsorption for a set time, washing away a residual matrix on the surface of the enrichment probe after the adsorption is completed, then fixing the enrichment probe at a mass spectrum injection port, and keeping a set distance between the tip of the enrichment probe and the mass spectrum injection port; the charged desorption solution continuously flows onto the enrichment probe to wet the enrichment probe, stable spray is formed at the tip of the enrichment probe, the adsorbed tetrachlorobisphenol A is desorbed and ionized under the combined action of an electric field and the desorption solution, and the obtained ions directly enter a mass spectrometer to be detected. The MIP is directly fixed on the surface of the glass fiber to construct a functional coating capable of being used for continuous flow desorption ionization mass spectrometry detection, so that rapid enrichment and ionization of target molecules are realized.

The utility model discloses a rapid wiring mechanism for an ion source electrode of a thermal ionization mass spectrometer, which comprises a ceramic fixing frame, an electrode holder and a connecting bolt, the ceramic fixing frame is provided with a connecting part and an electrode holder mounting part, the electrode holder mounting part extends to form a mistake-proof bump, and the electrode holder mounting part is provided with a first connecting screw hole; the electrode holder comprises an electrode holder body and a plurality of probes, the plurality of probes are vertically fixed on the electrode holder body at intervals, the electrode holder body is provided with a groove matched with the mistake-proof bump and a second connecting screw hole matched with the first connecting screw hole, and the connecting bolt detachably penetrates through the first connecting screw hole and the second connecting screw hole; according to the utility model, the ceramic fixing frame and the electrode holder are ensured to have only one installation direction by utilizing the matching of the error-proof convex block and the groove and the matching of the first connecting screw hole and the second connecting screw hole, so that the possibility of installation errors of the ceramic fixing frame and the electrode holder is avoided, and the installation process is simplified.

The invention discloses an expiration detection and health early warning technology based on online high-sensitivity mass spectrometry, and relates to the field of expiration detection and machine learning model algorithm application based on the online high-sensitivity mass spectrometry technology, and the technology comprises an expiration device, a flow velocity sensor, a cavity enhanced carbon dioxide monitor, a three-way high-speed electromagnetic valve and a constant-pressure dichloromethane generator, the device comprises a micro-pore electromagnetic valve, a mass spectrum sample inlet, an on-line high-sensitivity mass spectrum, a dichloromethane tail gas absorption bottle and an intelligent diagnosis system, wherein the cavity enhanced carbon dioxide monitor is used for realizing accurate judgment and sample introduction of alveolar gas; according to the online high-sensitivity mass spectrum, a VUV light source is adopted, and a gas sample obtained after dichloromethane and exhaled gas of a subject are mixed is ionized in an association ionization area; according to the method, the problems of limited sensitivity and coverage range in the prior art are solved, the variety and concentration of the volatile organic compounds in the exhaled gas of the human body are identified through an innovative association ionization mass spectrometry technology, and health condition analysis of a subject is realized by means of an intelligent diagnosis system provided with an interpretable machine learning model. Besides, the technology has the functions of detection of volatile organic compounds in expired gas of a human body and intelligent diagnosis and early warning of human health, can reflect the human health condition through a non-invasive means, and has important application prospects in the aspects of precise medical treatment of serious diseases and early screening of susceptible people.

The invention discloses a serum metabolism marker combination for ectopic pregnancy diagnosis and rupture risk prediction and application of the serum metabolism marker combination, and relates to the technical field of biological science. The metabolic fingerprint diagnosis model (AUCgt, 0.91) and the metabolic marker panel (AUCgt, 0.92) have the advantages that the performance of the metabolic fingerprint diagnosis model (AUCgt, 0.91) and the performance of the metabolic marker panel (AUCgt, 0.92) are obviously superior to those of an existing'ultrasonic + beta-hCG 'diagnosis mode, the problems of diagnosis delay, misdiagnosis and missed diagnosis clinically at present are effectively solved, and the clinical rigid requirements of early and accurate diagnosis are met; the fracture risk prediction model AUC 0.885 constructed by the method disclosed by the invention fills the current clinical blank. Key decision support can be provided for doctors, early intervention on high-risk patients is achieved, excessive medical treatment on low-risk patients is avoided, and the mortality risk of pregnant and lying-in women is reduced. According to the invention, based on the nanoparticle-assisted laser desorption / ionization mass spectrometry technology, ultra-fast detection of only about 3 seconds for single sample detection is realized, and the method is expected to become a fast detection tool in emergency treatment and outpatient service scenes.

The invention provides an irradiation hazardous chemical trace multi-mode joint inspection method and system, and relates to the technical field of intelligent monitoring of hazardous chemical trace detection.The method comprises the steps that three paths of original response signals of electrochemical impedance, surface enhanced Raman and plasma-assisted ionization mass spectrometry arranged in an irradiation environment are obtained, and a multi-mode characteristic matrix is obtained; performing Tucker decomposition to obtain a core tensor and a factor matrix, and performing interpretability screening and vectorization to form a low-dimensional fusion feature vector; constructing an attribute graph by taking the low-dimensional fusion feature vector as mutual information, and outputting a trace hazardous chemical category label and a concentration estimated value through three-layer graph attention network embedding; substituting the category label and the concentration estimated value into the ERPG parameter to generate a digital twinning risk map; and driving ventilation, isolation and spraying actuators to complete closed-loop response through a federal learning consensus risk map. The invention provides an efficient and reliable technical scheme for hazardous chemical trace detection and safety management and control in the irradiation environment.

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method includes: a) at least one imaging step, including imaging at least one reflective target (128) to which a sample containing at least one analyte is applied, using at least one imaging device (235); b) at least one sample recognition step, including locating at least one sample area on the reflective target (128); and c) at least one analyte detection step, including detecting at least one analyte in the sample using surface-assisted laser desorption / ionization mass spectrometry (SALDI-MS) in a laser desorption mass spectrometer (220), wherein laser radiation is applied to the reflective target (128) using at least one laser source (222) of the laser desorption mass spectrometer (220), and the laser radiation is directed to the located sample area using at least one control device (237), such that at least one ion of the at least one analyte is generated, and the ion is detected using at least one of a mass analysis unit (224) of the laser desorption mass spectrometer (220) or an ion mobility spectrometer.

The invention discloses a method for rapidly identifying luxury fiber components. The method comprises the following steps: (A) pretreating an actual sample; (B) detecting the sample by adopting a screen mesh collision-microtube plasma ionization mass spectrometry to obtain mass spectrum data; and (C) comparing the obtained mass spectrum data with a random forest classification model to obtain an identification result. The invention further discloses an establishment process of the random forest classification model. The method has high customizability and instrument compatibility, experimental operation is simple, the ionization process is stable, data consistency is good, the requirement of customs for on-site rapid detection of textiles can be met, and a feasible technical means is provided for data transverse comparison, cross-laboratory standardized large database establishment and the like.

The invention discloses a method for preparing an electroplating template and electroplating. The method comprises the following steps: depositing a first insulating material layer on a metal oxide glass substrate; forming a photoresist stacking structure on the first insulating material layer; patterning the photoresist layer in the photoresist stack structure so as to convert the photoresist layer into a columnar structure array; sequentially etching the second insulating material layer and the anti-reflection coating below the photoresist layer by using the photoresist layer to form a three-dimensional pattern structure; depositing a metal layer on the first insulating material layer and the three-dimensional pattern structure; the anti-reflection coating is subjected to stripping treatment, part of the metal layer on the anti-reflection coating is removed together, and the remaining metal layer becomes a metal pattern layer; etching the first insulating material layer by using the metal pattern layer so as to form a periodic hole array with depth; removing the metal pattern layer to obtain an electroplating template; and performing metal electroplating by using the electroplating template to form the metal nanostructure array substrate.

The present invention discloses a method and device for substance analysis by directly integrating a digital microfluidic chip and matrix-assisted laser desorption / ionization mass spectrometry. In the device of the present invention, the digital microfluidic system includes a digital microfluidic chip and a micro-droplet driving platform. The chip includes an upper plate, a lower plate and a conductive gasket. The upper plate includes an upper substrate and a conductive layer. A hydrophobic layer is provided on the lower surface of the conductive layer, and a number of hydrophilic sites are arranged at intervals on the surface of the hydrophobic layer. Each hydrophilic site is a pit recessed towards the conductive layer. The lower plate includes a lower substrate and a dielectric layer. An electrode array is arranged between the lower substrate and the dielectric layer, and a lower hydrophobic layer is provided on the upper surface of the dielectric layer. The matrix-assisted laser desorption / ionization mass spectrometry includes a target plate substrate, and a groove for placing the upper plate of the chip is provided on the upper surface of the target plate substrate. By directly coupling the upper plate provided with hydrophilic sites and the customized target plate and then sending them into the mass spectrometer, the present invention can reduce manual operations and achieve automated analysis, and has the potential for high-throughput, sensitive and specific analysis.