74 results about "Ionization mass spectrometry" patented technology

The invention discloses a double-pulse system and method for improving depth resolution of laser ionization mass spectrometry, and relates to the technical field of mass spectrometers, the system comprises a control system for controlling femtosecond laser parameters generated by a femtosecond laser and nanosecond laser parameters of a nanosecond laser; the laser energy regulation and control module is used for carrying out phase adjustment and detection on the input femtosecond laser and outputting the femtosecond laser meeting set parameters based on the femtosecond laser parameters; the light beam shaping and light beam morphology analysis module is used for shaping femtosecond laser and focusing the femtosecond laser on the surface of the sample for laser denudation; the nanosecond laser performs off-resonance multi-photon ionization on the denudated neutral particles to generate ionized ions for mass spectrometry. According to the invention, the depth resolution of the laser ionization mass spectrum can be improved to nanoscale, the ionization efficiency of ions is improved under the condition of reducing the sampling amount, and meanwhile, the sensitivity of an instrument and the accuracy of quantitative analysis are improved.

The invention provides a non-invasive real-time detection method and system for characteristic substances of yin and yang deficiency syndromes of a human body, and relates to the technical field of substance detection.The specific scheme includes the steps that an exhaled gas sample containing life substances carrying physiological or pathological information is collected; analyzing the exhaled gas sample based on electrospray extraction ionization mass spectrometry, and marking exhaled gas characteristic molecule information to obtain a yin-yang data set; constructing an initial model by combining a random forest and a gradient boosting decision tree algorithm, training and testing the initial model based on the yin-yang data set to obtain a yin-yang identification model, and associating yin-yang attributes of traditional Chinese medicine diseases with exhaled gas characteristic molecule information; real-time exhaled gas of the subject is obtained, exhaled gas characteristic molecule information is detected, the yin-yang deficiency syndrome is recognized based on the yin-yang recognition model, and a yin-yang deficiency syndrome detection report is obtained and output. The invention aims to solve the problems that the traditional static analysis is difficult to directly distinguish yin and yang of a human body and the accuracy is relatively low by utilizing high-latitude mass spectrum data.

This invention relates to a method for detecting the activity of a toxin produced by bacteria of the families Peptostreptococcaceae or Clostridiaceae using mass spectrometry, which comprises the following steps: a) providing a planar surface suitable for mass spectrometry measurement, with attached affinity molecules; b) incubating the planar surface from step a) with the substrate of the toxin to be detected, to bind the substrate to the affinity molecules; c) subsequently incubating the planar surface from step b) with a sample in which the active toxin is to be detected, in the presence of a modification compound, and incubating it for a time sufficient for the substrate to react with the modification compound in the presence of the active toxin, if present in the sample, to form a modified substrate if the active toxin is present in the sample; d) removing the remnants of the sample; e) subjecting the (modified) substrate on the planar surface to matrix-assisted laser desorption / ionization, ionizing it and subsequently measuring the mass spectrum of the (modified) substrate; f) analyzing the measured mass spectrum, such that if the measured mass spectrum corresponds to the non-modified substrate, the active toxin is not present, and if the measured mass spectrum corresponds to the modified substrate or a mixture of the modified and non-modified substrate, then the active toxin is present in the sample. The invention further relates to a device for the soft landing of affinity molecule ions onto a planar surface suitable for mass spectrometry for carrying out step a) of the above method.

The utility model discloses a Faraday cup of an ion receiver of a thermal ionization mass spectrometer, which comprises a non-metal receiving support, a non-metal receiving electrode and a pair of external fixing metal plates, the non-metal receiving support is concaved inwards to form a notch communicated with the outside, the non-metal receiving electrode is arranged in the notch, and the external fixing metal plates are fixed on the non-metal receiving support. The pair of external fixing metal plates are oppositely fixed on the two side surfaces of the non-metal receiving bracket, and the external fixing metal plates and the non-metal receiving bracket are encircled to enable the notch to only have a state that the top is communicated with the outside, so that the whole Faraday cup retains the rigidity of metal, the outside is not easy to deform, and the service life of the Faraday cup is prolonged. The non-metallic material is adopted in the device, so that the device has the characteristic of not rebounding ions, interference on ion measurement can be effectively reduced, and the measurement accuracy is improved.

To provide a method and system for protein characterization using online chromatography and electrospray ionization mass spectrometry.SOLUTION: A method for protein characterization comprises the steps of: contacting a sample including protein to a chromatography system with a size exclusion chromatography resin; cleaning the size exclusion chromatography resin using a mobile phase to obtain an eluent containing the protein; and characterizing the protein in the eluent using an electrospray ionization mass spectrometer under non-denaturing conditions.SELECTED DRAWING: Figure 2

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

This provides a method for identifying S. aureus and other bacterial species that make up the coagulase-negative staphylococcus group. [Solution] A method for determining the presence or absence of Staphylococcus species in a species-specific manner, wherein the Staphylococcus species is one or more species selected from the group consisting of S. aureus, S. epidermidis, S. capitis, S. caprae, S. hominis, and S. lugdunensis, and comprises a peak detection step of determining whether one or more peaks selected from a group consisting of peaks corresponding to specific m / z values ​​are detected in a mass spectrum obtained using matrix-assisted laser desorption / ionization mass spectrometry on a test sample with respect to S. aureus, and a bacterial species determination step of determining the presence or absence of S. aureus based on the peak detection result of the peak detection step.

The invention discloses a nano-droplet interface ionization mass spectrum ion source device and an operation method thereof, and relates to the field of nano-droplet interface ionization mass spectrum ion source devices, the device comprises a nano-spray needle used for loading a sample solution and realizing a nano-spray process; the metal electrode is inserted into the nano-spray needle, is in direct contact with the sample solution and is used for providing a high-voltage electric field required by nano-droplet interface ionization for the sample solution; and the ionization chamber is pre-filled with inert auxiliary gas and is used for protecting the interface ionization process of the nano liquid drops. High-sensitivity detection of polycyclic aromatic hydrocarbon in samples such as small-size single cells is realized, and higher signal intensity is obtained; better detection response to weak polar compounds (especially compounds with aromatic skeleton structures) in a mixed sample is realized; the detection of the weak polar compound polycyclic aromatic hydrocarbon in the single cell is realized.

This invention provides a method for detecting dichloronaphthalene, a persistent organic pollutant in soil sediments. The method includes: collecting soil sediment samples, pre-treating them by freeze-drying, grinding, and sieving to obtain a sample; extracting and purifying the sample using an organic solvent to prepare a test solution; analyzing and detecting the sample using ultra-high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (UPLC-APCI-MS / MS), and calculating the measured value of dichloronaphthalene in the test solution by substituting the obtained peak area and fractionation ratio into a specific formula. This invention fills the gap in my country's technology for detecting dichloronaphthalene-type persistent organic pollutants in soil sediments. This method can effectively eliminate interference from complex matrices, accurately determine trace amounts of six dichloronaphthalene isomers, and has the advantages of high sensitivity, strong anti-interference ability, simple operation, and high detection accuracy.

A mass spectrometry method according to the present disclosure is a mass spectrometry method in a method for discriminating whether a polyethylene terephthalate fiber contained in a target fiber is a recycled fiber. The mass spectrometry method includes a step of preparing a sample and a step of measuring the sample using matrix-assisted laser desorption / ionization mass spectrometry. The step of preparing the sample has a step of dissolving the target fiber in a first solvent to prepare a solution, a step of adding tetrahydrofuran to the solution to separate the solution into a precipitate and a supernatant, a step of obtaining the supernatant, and a step of preparing a mixed solution containing the supernatant and a matrix on a sample plate.

The invention discloses an application of a benzophenone derivative as a bipolar matrix-assisted laser desorption ionization matrix in mass spectrometry detection. The benzophenone derivative matrix provided by the invention can effectively improve desorption ionization efficiency and reduce background interference, is compatible with a positive ion and negative ion bipolar detection mode, and solves the technical bottleneck of single polarity of a traditional matrix detection mode. The matrix has a wide-spectrum absorption characteristic in an ultraviolet region, can be adapted to laser wavelengths of various matrix-assisted laser desorption ionization mass spectrometers, and has a wide application prospect. Besides, when the matrix is used for lipid compound detection, lipid endogenous metabolite spectrum signals with high sensitivity and high coverage rate can be obtained in positive and negative ion modes, the matrix is expected to be widely used for metabonomics and clinical basic research, and key technical support is provided for high-sensitivity and bipolar imaging of small molecule compounds in complex biological samples.

The invention relates to an application of elemental carbon in laser desorption ionization mass spectrometry detection, and the elemental carbon (EC) is obtained by cleaning and extracting components of organic carbon (OC) by using an incomplete combustion product Soot. The EC is used as a material of a graphite-like structure, is used as a substrate when environmental pollution particles are analyzed by using LDI-MS, and can enhance the detection strength of aromatic compounds in the particles. Furthermore, when the EC is used for analyzing aromatic substances, the EC has a better enhancement effect on substances containing reactive hydrogen (PAH with OC containing odd carbon in the invention, namely PAHodd) and substances with lower conjugacy. According to the device and the method, pollution particulate matters are recycled, detection and analysis of particulate matter components are facilitated, and the detection sensitivity of aromatic substances is improved.

The invention discloses an MOF-derived heterojunction, a preparation method thereof and application of the MOF-derived heterojunction in metabolism detection, and relates to the technical field of heterojunction material preparation. The preparation method comprises the following steps: performing first-stage heat treatment on an MOF precursor to prepare a metal oxide, and reacting the metal oxide with a titanium source to obtain a product; or directly reacting the MOF precursor with a titanium source to obtain a product; and carrying out second-stage heat treatment on the product in an inert atmosphere or air to obtain the MOF-derived heterojunction. The invention relates to application of MOF-derived heterojunction as a matrix in detection of serum metabolic fingerprints by laser desorption / ionization mass spectrometry and application in construction of a disease diagnosis model. The heterojunction material prepared by the invention has the advantages of high ionization efficiency, good photothermal effect and strong biological matrix interference resistance, and can be used as an LDI MS nano matrix to be applied to respiratory disease serum metabolism fingerprint diagnosis and disease diagnosis models so as to distinguish respiratory diseases.

The invention belongs to the technical field of ion detection, and particularly relates to a method and a system for screening ions in chemical ionization mass spectrometry. The screening method comprises the steps that an ion screening rod is arranged on an ion transmission path of an ion transmission area of the multistage differential chemical ionization mass spectrometer, main radio frequency voltage is applied to the ion screening rod, and full-time broadband control waveforms are coupled; the main radio frequency voltage is used for realizing collision cooling and radial focusing of ions; the full-time broadband control waveform is a broadband excitation waveform of a time domain and a frequency domain, the frequency of the full-time broadband control waveform is the same as the resonant frequency of reagent ions, and the full-time broadband control waveform is used for removing excessive reagent ions. Due to the fact that flying ions are restrained by a radio frequency electric field, the ions with different mass-to-charge ratios have specific resonant frequencies, when a full-time broadband waveform with the same resonant frequency as the reagent ions is externally applied, the reagent ions can be ejected out and annihilated on the ion screening rod, and therefore real-time screening of the reagent ions is achieved.

The present invention belongs to the field of analytical chemistry, and particularly relates to an in-situ ionization mass spectrometry system and an analysis method. The in-situ ionization mass spectrometry system includes an ionization device, a mass spectrometry device, and a sample platform disposed between the two. The sample platform includes a sample plate and a fixing device. The sample plate is used to support the sample to be measured, and the fixing device is used to fix the sample plate and enable the sample plate to move in the horizontal direction. The in-situ ionization mass spectrometry system of the present invention can not only measure samples continuously and multiple times, but also be applicable to samples of different shapes and sizes, and has the advantages of high detection efficiency and good universality. And by setting a flow limiting member at the gas flow outlet of the ionization device, the metastable particle flow generated by the ionization device can be concentrated and led to the surface of the sample to be measured, improving the resolution of surface analysis of the sample; by setting the inlet transfer tube of the mass spectrometry device as a bent tube with different angles, the ionized compound to be measured can be efficiently transmitted into the mass spectrometry device for real-time analysis.

The present invention provides matrices for use in mass spectrometry, particularly matrix-assisted laser desorption / ionization mass spectrometry techniques. Methods of use, including for imaging mass spectrometry, of the presently disclosed matrices are also provided.

The invention provides a novel on-line extraction and sequential ionization mass spectrometry device (S-oEESI-MS). The device comprises a multi-solvent pump parallel structure, a sample extraction bin and an electrospray ion source. The device can be used for sequential online extraction and sequential ionization characterization of substances with different polarities in a complex sample. Compared with the traditional LC-MS (liquid chromatography-mass spectrometry) and oEESI-MS (oEESI-MS), the device has more accurate distinguishing and identifying capability. The invention also provides the application of the on-line extraction sequential ionization mass spectrometry device (S-oEESI-MS) in the production area analysis and / or detection of the traditional Chinese medicine bighead atractylodes rhizome. Mass spectrum information obtained by the device is subjected to multivariate statistical analysis, so that highly similar samples from different producing areas can be completely distinguished. The method has wide applicability and popularization value for quality control and standardized development of traditional Chinese medicines.

1. The name of this design product: Medical direct ionization mass spectrometer. 2. Purpose of the product of this design: Direct ionization mass spectrometry technology is used to ionize the test substance in the test sample, and the concentration of the substance is quickly tested and analyzed through the mass spectrometry detector to provide supporting data for medical diagnosis. 3. The key point of the design of this product lies in the three-dimensional image. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention discloses a non-target analysis technology based on mass spectrum similarity, differential spectrum distribution and time-dependent hierarchical clustering analysis (HCA), is used for analyzing time-dependent mass spectrum data obtained by direct sample introduction of a high-resolution flight time chemical ionization mass spectrometer and realizing rapid identification of volatile components with different white spirit characteristics, and belongs to the technical field of analysis and detection. According to the method, the similarity of volatile components of Baijiu is judged through characteristic included angles between mass spectrograms, and Baijiu with different flavors or different series of Baijiu with the same flavor is qualitatively distinguished; further acquiring species and abundance differences of the volatile components in combination with differential spectrum distribution; the time-dependent HCA method is used for carrying out two-dimensional analysis of time and component latitude on the high-resolution mass spectrum, so that characteristic volatile species of different Baijiu are accurately identified, and efficient and rapid distinguishing and identification of Baijiu brands or aroma types are realized. According to the method, multi-level analysis is carried out on the mass spectrum collected in situ, the mass spectrum difference of different brands or flavors of baijiu is visually displayed, online detection and rapid identification of the volatile components of the baijiu can be achieved, the problem of refined identification of the volatile components of the baijiu is solved, and the method is widely suitable for baijiu quality control, authenticity identification and flavor database construction.

The application provides a lung cancer intelligent screening model construction method and system based on sputum direct mass spectrum data. The method comprises the following steps: S1, obtaining the original mass spectrum data of the sputum sample detected by neutral desorption electrospray extraction ionization mass spectrometry, preprocessing including mass spectrum peak detection, peak alignment and intensity normalization to form an initial feature matrix; S2, extracting a core metabolic fingerprint feature set from the initial feature matrix; S3, constructing and training a heterogeneous integrated diagnosis model; S4, optimizing and verifying the hyperparameters of the heterogeneous integrated diagnosis model, and packaging the optimized heterogeneous integrated diagnosis model and the screening rules of the core metabolic fingerprint feature set into a lung cancer screening deployment module. The application realizes high-robustness, high-precision and high-explainability lung cancer non-invasive intelligent screening.

At least one molecule is ionized and a mass spectrometer mass analyzes an m / z range, producing an m / z mass spectrum. A range of N sequential charge states is received. A copy of the m / z mass spectrum is created for each of the N charge states, producing N m / z spectra. Each spectrum of the N spectra is converted to a neutral mass mass spectrum using a different charge state of the N charge states, producing N neutral mass mass spectra. The N neutral mass mass spectra are aligned by neutral mass. When two or more spectra of the N neutral mass mass spectra corresponding to two or more different and sequential charge states include a neutral mass peak above a predetermined intensity threshold at a neutral mass value within a predetermined neutral mass tolerance, the neutral mass value is identified as a neutral mass of the at least one molecule.

This invention relates to the field of industrial quality control and rapid mass spectrometry analysis technology, and discloses a method, device, and system for rapid in-situ / environmental ionization mass spectrometry screening and split determination of target residues in industrial samples. This method addresses time-sensitive scenarios such as incoming material acceptance, process anomaly location, on-site inspection, and emergency splitting. It tackles the challenges of complex industrial sample matrices, low target analyte content, and the need for enrichment and long cycles for confirmatory detection. The method constructs a closed-loop process: "minimalist sampling / introduction – in-situ / environmental ionization – mass spectrometry acquisition – background reference – candidate ion set – matching and exclusion discrimination – splitting decision output." The method acquires sample and background reference spectra within the same acquisition time window or equivalent time window. Candidate feature ion sets are extracted through background subtraction / difference spectroscopy. Combined with repeatability stability, sample / background contrast thresholds, and isotope / adjunct / accompanying ion consistency rules, reference fingerprint database matching and environmental background / non-target spectral exclusion are performed. The system outputs a screening conclusion of "suspected presence / suspected absence / requires verification" and provides triage suggestions for further confirmation (GC-MS, LC-MS / MS, or tandem mass spectrometry). The system integrates sampling / introduction, ionization, mass spectrometry detection, and data processing decision modules. It is adaptable to gas / liquid / solid samples, covering volatile and transpolar molecular systems, and offers advantages such as high speed, low preparation cost, engineerable deployment, and reduced risk of misjudgment.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The invention discloses an acanthosphere-shaped multi-interface type metal oxide magnetic particle and a preparation method and application thereof.The magnetic particle comprises a core composed of magnetic nanoparticles and a shell layer composed of titanium oxide nanosheet spines arranged in a radial mode, and the magnetic particle has superparamagnetism, high specific surface area and rich active sites; and a large number of trap spaces matched with the exosome scale range, so that the method can be used for efficiently enriching and separating the exosome from the biological fluid. Meanwhile, the magnetic core has an out-phase junction composed of amorphous phase titanium oxide and anatase phase titanium oxide, a heterojunction interface composed of titanium oxide and the magnetic core and an ordered spine microstructure, the synergistic effect of the multi-interface effect and the point effect is fully exerted under laser irradiation, the excellent ultraviolet absorption capacity and electron-hole separation efficiency are shown, and the magnetic core has the good application prospect. The method is used for laser desorption ionization mass spectrometry, efficient in-situ pyrolysis of the exosome is achieved, desorption and ionization of metabolite in a lysis solution are promoted, and a high-resolution mass spectrum of the metabolite of the exosome is obtained.

The invention belongs to the field of chemical analysis and detection, and particularly relates to a research method for real-time analysis of a molecular mechanism of gold nanoparticles against bacteria by nanoESI-MS. The method comprises the following steps: performing mixed culture on the gold nanoparticles and bacteria, performing centrifugation, collecting supernate, taking the supernate as a sample solution, and performing analysis by using nanoESI-MS to obtain spectrogram data; performing orthogonal partial least square discriminant analysis on the spectrogram data, and establishing a metabolic substance and sample category prediction model; screening differential metabolites based on the prediction model and projection variable importance; and constructing a correlation network diagram based on the differential metabolites, and analyzing the antibacterial mechanism of the gold nanoparticles. According to the method, analysis can be carried out without sacrificing the integrity of the sample, so that the defects caused by the use of an extracting agent in the traditional mass spectrometry technology are avoided, and the analysis efficiency and the coverage range are also improved.

A matrix solution used to be mixed with a sample in a matrix-assisted laser desorption / ionization mass spectrometry method. The matrix solution is an aqueous solution containing acetonitrile, ethanol, trifluoroacetic acid, and water. A content of the acetonitrile is 30 to 40 vol% based on the matrix solution. A content of the ethanol is 10 to 20 vol% based on the matrix solution. A content of the trifluoroacetic acid is 1 to 3 vol% based on the matrix solution. The matrix solution contains α-cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid, or sinapinic acid in a ratio of 5 to 20 mg / ml.

The invention belongs to the field of mass spectrum ionization, and particularly relates to a photoinduced Penning ionization mass spectrum method. Photoelectrons generated by a photoelectric effect accelerate in an electric field to obtain enough energy, helium He, neon Ne and argon Ar in ground states are excited to metastable states, and the energy of the metastable states of the neutral atoms is higher than 10.6 eV (He excitation energy is 20.62 eV and 19.82 eV); the excitation energy of Ne is 16.72 eV, and the excitation energy of Ne is 16.62 eV; the excitation energy of Ar is 11.72 eV and 11.55 eV), sample molecules with ionization energy higher than photon energy and incapable of being ionized by traditional photoionization can be ionized through Penning ionization, so that the reduction of mass spectrum resolution, sensitivity and quantitative capacity caused by a space charge effect caused by coulomb repulsion among too strong reagent ions in an ion transmission process is avoided, and the detection sensitivity is improved. And damage of too strong reagent ions to the ion detector. The sensitivity can be improved by about 5 times for sample molecules ionized by traditional photo ionization energy, such as toluene, with ionization energy lower than photon energy.

The application discloses a nanoliter spray ionization mass spectrometry interface device, an analytical instrument and an ionization method, and belongs to the technical field of electrospray mass spectrometry ionization devices. The nanoliter spray ionization mass spectrometry interface device comprises an ion guide tube, a first electrode and a second electrode; the inner wall of the ion guide tube is plated with a conductive film; the ion guide tube has an ion transmission channel in the form of a funnel along the length direction of the tube; the ion transmission channel has a first end and a second end; the diameter of the ion transmission channel gradually decreases from the first end to the second end; the first electrode is connected to the end of the first end; and the second electrode is connected to the end of the second end. The device has a simple structure and is convenient to operate; high stability, high sample utilization and high ion transmission efficiency of the electrospray process under a nanoliter flow rate can be realized; positive and negative switching can be directly and rapidly performed under the condition of normal pressure and opening; and problems, such as unstable spraying caused by bad high pressure and complex parameter optimization process, are avoided.

The utility model discloses an anti-splashing thermal ionization mass spectrometer liquid nitrogen liquid inlet hopper, which relates to the technical field of thermal ionization mass spectrometry and comprises a liquid inlet shell, a plurality of limiting blocks are fixedly connected to the inner wall of the liquid inlet hopper shell close to the top end, a curved-surface funnel is movably connected to the inner wall of a cushion block, a liquid inlet is formed in the bottom of the curved-surface funnel, and a liquid outlet is formed in the bottom of the curved-surface funnel. A first flow guide plate is movably connected to the inner wall of the liquid inlet, a flow guide disc is fixedly connected to the bottom of the curved-surface funnel, a plurality of second flow guide plates are fixedly connected to the inner wall of the liquid inlet hopper shell, a filtering opening is formed in the bottom of the liquid inlet hopper shell, and a filtering hopper is movably connected to the top end of the filtering opening. The lower surface of the curved-surface funnel stops part of splashing liquid, the liquid flows along the gap between the outer wall of the flow guide plate and the flow guide plate, and when the internal liquid reacts, smoke is guided along the arc-shaped curved surface and the gap of the flow guide plate, so that the smoke flows towards the direction of the opposite liquid pouring surface, and the influence of the smoke on the liquid inlet efficiency is reduced.

This invention discloses a mass spectrometry patch for wound infection detection and its application, relating to the field of biomedical device technology. The mass spectrometry patch consists of a contact layer, a collection layer, and a protective layer from bottom to top. The contact layer is a polycarbonate mesh film, the collection layer is a polyvinylidene fluoride nanofiber membrane with high-density gold nanoparticles grown in situ on its surface, and the protective layer is a polyurethane film. This invention provides a wearable mass spectrometry patch integrating sampling and detection, achieving non-invasive sampling and direct mass spectrometry analysis. A single detection using laser desorption / ionization mass spectrometry can obtain a global metabolic fingerprint containing hundreds of metabolites, which simultaneously contains pathogen species-specific signals and host metabolic biomarker signals. By training an AI model to analyze the complex fingerprint, rapid pathogen identification and dynamic monitoring of host responses (such as lactic acid and glucose) can be completed simultaneously within 5 minutes, achieving decoupling of pathogen information from changes in host biomarkers.