Method for directly analyzing lung cancer tissue sample based on tissue electro-spray ionization mass spectrometry

Abstract

A method for directly analyzing a lung cancer tissue sample based on the tissue electro-spray ionization mass spectrometry concretely comprises the steps that an electro-spray extraction ionization source in the tissue sample is set up; an extraction agent, namely, methyl alcohol is sucked through a micro-syringe connected with a quartz capillary, the extraction agent is dripped on the surface of the sample through the quartz capillary, and an injection pump controls the micro-syringe; the lung cancer tissue sample is cut into a piece of which the volume is 1 mm3 and placed at the tip of an acupuncture needle, and the needle tip is inserted into the sample and keep unexposed; the distance between the front end of the sample and an inlet of a mass spectrometer is adjusted, and the tail end of the acupuncture needle, the front end of the sample and the inlet of the mass spectrometer are adjusted to be in a horizontal coaxial state; DC voltage is applied on the acupuncture needle, so that extraction, desorption and ionization happen on the sample, and the sample generates spray which enters the mass spectrometer. According to the method, the information of molecules in the sample can be directly and quickly obtained, and the result shows that the method is a mass spectrometry method which has the advantages that the device is simple, manufacturing cost is low, ionization efficiency is high, and damage to the sample is small.

Description

technical field [0001] The present invention relates to a tissue spray ionization mass spectrometry (TSi-MS) direct analysis method, in particular to a method based on a simple ionization device to realize the ionization of human cancer tissue samples and perform rapid direct mass spectrometry analysis on unpretreated human cancer tissue samples method. Background technique [0002] Mass spectrometry (MS) technology is a universal method with high sensitivity, good specificity and fast response speed among many modern analytical testing technologies. A biological tissue sample is an extremely complex organism composed of cells and intercellular matrix, which contains rich biological information, and is the basis for pharmacology, proteomics, lipidomics, and pathophysiology studies. With the development of life sciences, biological tissue samples have developed from traditional morphological observation and tissue composition research to pathophysiological research, which ur...

AI Technical Summary

Problems solved by technology

With the help of the high-efficiency separation ability of chromatographic technology for complex samples, the effective analysis of complex samples can only be achieved through mass spectrometry technology. However, although these technologies solve the problem of mass spectrometry analysis of biological tissues under normal pressure, they cannot break away from the complex sample analysis. The pretreatment process of the lock, and the sample volume required for analysis is large, which cannot realize the rapid, online, high-throughput detection of complex samples

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