308 results about "Electro spray" patented technology

A nanofiber membrane is formed on a microfiber membrane. The nanofiber membrane may be electro sprayed directly onto the microfiber membrane and becomes integrated with the microfiber membrane to form a filter. The microfiber membrane provides structural integrity to for the nanofiber membrane, and an additional microfiber membrane may be added to sandwich the nanofiber membrane.

Improvements have been made for collection and focusing of ions generated from atmospheric pressure sources such as electrospray, atmospheric pressure chemical ionization, inductively coupled plasma, discharge, photoionization and atmospheric pressure matrix assisted laser desorption ionization. A high transmission electro-optical surface is placed between the source regions and the focusing regions to optimize the field geometries and strengths in each respective region. Compression ratios of greater than 5000 are capable of transferring virtually all ions from large volume dispersive ion regions into ion beam cross-sections of less than 1 mm. Embodiments of this invention are methods and devices for improving sensitivity of mass spectrometry when coupled to atmospheric pressure ionization sources.

Electrospray systems and modified electrospray systems for the fabrication of core-shell particles for controlled-release and/or sustained-release treatment and delivery are herein disclosed. The electrospray system may include between one and a plurality of co-axially situated tubes. Each tube may be electrically connected to a power supply wherein a voltage may be applied thereto. Core-shell particles may be collected on a collection target, which may be a wet or dry collector, and electrically connected to the power supply. Core-shell particles and methods of manufacture are also disclosed. The precursors of the core-shell particles may be polymer- or biomacromolecule-based solutions and may include at least one treatment agent incorporated therein. The number of “core” particle(s) within the “shell” may vary and may provide different treatment agent release profiles depending on the material and/or chemical characteristics of the polymer and/or biomacromolecule used. Methods of treating a condition are also disclosed. A treatment may include delivery of a plurality of core-shell particles which include a treatment agent to a treatment site. Delivery may be performed by a surgical procedure or by a non-invasive procedure such as catheter delivery.

The invention discloses a general rapid detection method for micromolecular poisonous and harmful substances in liquid milk. The method is characterized in that the method concretely comprises the following steps: 1, carrying out simple and general extraction and purification on a liquid milk sample to obtain a loading liquid; 2, preparing a mixed standard solution and a matrix addition standard curve; 3, determining the micromolecular poisonous and harmful substances in the liquid milk through UPLC-MS/MS; and 4, carrying out concentration calculation. The method of the invention has the following advantages: the method starts from general characteristics of micromolecular compounds and fully takes the dissolvability, the thermal stability, the acidic and basic stability, the polar strength, the coexistent stability and the like of all the substances into consideration, a general rapid preprocessing technology and a UPLC-MS/MS electro-spray apparatus determination technology are developed, and the substantial efficiency improvement and the detection cost reduction effect are brought to the production process control and food detection work on condition that detection limit requirements are satisfied.

Methods are provided according to aspects of the present invention for making starch fiber or particle compositions by wet-electrospinning or wet-electrospraying including providing a solution or dispersion of starch in an aqueous or non-aqueous solvent or dispersant, where the starch is present at a concentration above the critical entanglement concentration, with the proviso that the aqueous or non-aqueous solvent or dispersant does not consist only of water; heating the solution or dispersion of starch to a temperature above the crystallization temperature of the starch; electro spinning or electro spraying the heated solution or dispersion of starch to produce starch fibers or starch particles, respectively; and contacting the starch fibers or starch particles with a coagulation bath fluid.

The invention relates to a preparation method for a nano-granular solid dispersion of a hydrophobic drug by high-voltage electrostatic spraying, which comprises the following steps of: mixing a hydrophobic drug, a drug-carrying polymer and an organic solvent at a mass ratio of (1-10): (10-20): (80-94) to prepare a consolute electric spraying solution; and then carrying out high-voltage electrostatic spraying while controlling the flow rate of the consolute electric spraying solution at 0.5-2.5 mL/h, the distance between a receiving board and a wire spraying port at 15-30cm and the voltage at 5-30kV to obtain the nano-granular solid dispersion of the hydrophobic drug. The preparation method is simple to operate, is low in cost, is environmentally-friendly and is suitable for industrial production; and the prepared solid dispersion of the hydrophobic drug not only can highly disperse a composite of a polymer and the drug into an amorphous state and has a nano-structural characteristic.

A method for directly analyzing a lung cancer tissue sample based on the tissue electro-spray ionization mass spectrometry concretely comprises the steps that an electro-spray extraction ionization source in the tissue sample is set up; an extraction agent, namely, methyl alcohol is sucked through a micro-syringe connected with a quartz capillary, the extraction agent is dripped on the surface of the sample through the quartz capillary, and an injection pump controls the micro-syringe; the lung cancer tissue sample is cut into a piece of which the volume is 1 mm3 and placed at the tip of an acupuncture needle, and the needle tip is inserted into the sample and keep unexposed; the distance between the front end of the sample and an inlet of a mass spectrometer is adjusted, and the tail end of the acupuncture needle, the front end of the sample and the inlet of the mass spectrometer are adjusted to be in a horizontal coaxial state; DC voltage is applied on the acupuncture needle, so that extraction, desorption and ionization happen on the sample, and the sample generates spray which enters the mass spectrometer. According to the method, the information of molecules in the sample can be directly and quickly obtained, and the result shows that the method is a mass spectrometry method which has the advantages that the device is simple, manufacturing cost is low, ionization efficiency is high, and damage to the sample is small.

The invention provides a method for rapid detection of multi-class pharmaceutical and personal care products and pesticides in water. The method includes: taking a to-be-detected water sample, and subjecting the to-be-detected water sample to pretreatment to obtain a to-be-detected sample; employing one or more groups of the following five groups of detection conditions for liquid chromatography-tandem mass spectrometry detection on the to-be-detected sample respectively so as to realize qualitative analysis and quantitative detection of multi-class pharmaceutical and personal care products and pesticides in the to-be-detected water sample: (a) the mobile phase is a formic acid-ammonium formate aqueous solution and acetonitrile, and the ionization mode is electrospray ionization positive mode; (b) the flow phase is a formic acid aqueous solution and acetonitrile, and the ionization mode is electrospray ionization positive mode; (c) the mobile phase is an acetic acid-ammonium acetate aqueous solution and acetonitrile, and the ionization mode is electrospray ionization negative mode; (d) the mobile phase is water and acetonitrile, and the ionization mode is electrospray ionization negative mode; and (e) the mobile phase is a formic acid-ammonium formate solution and acetonitrile, and the ionization mode is electrospray ionization positive mode.

A method of making a polymer membrane, the method including providing a first monomer solution having a first solvent, a second monomer solution having a second solvent, and a substrate having a surface, and including electrospraying the first monomer solution onto the substrate surface and electrospraying the second monomer solution onto the substrate surface to form the polymer membrane on at least a portion of the substrate surface.

The invention discloses a constant-temperature high-voltage electrostatic spray device and a method preparing polymer medicine-carrying nanoparticles by using the same. The device comprises an axial flow injection pump, an injector, a metal capillary type syringe needle, a high-voltage power supply, a powder receiving plate and a constant-temperature control device. The method for preparing the polymer medicine-carrying particles comprises the following steps of: dissolving active ingredients such as a polymer, a medicine and the like together in the same solvent to be mixed to form a consolute electrical spraying solution; then filling the obtained consolute electrical spraying solution in the injector and mounting the injector on the axial flow injection pump, switching on a power supply of an electric heating film, performing high-voltage electrostatic spraying in a high-voltage electric field after the temperature raises to a range of 40 to 80 DEG C thus the polymer medicine-carrying nanoparticles are obtained. As the automatic constant-temperature control device is added in the process in the invention, the polymer medicine-carrying nanoparticles can be prepared through single-step electrical spraying. The method has a simple preparation process, is easy to regulate and control, and is suitable for industrialized production; and the obtained polymer medicine-carrying nanoparticles have good dispersibility, and controlled release performance which can meet the design requirement.

The invention discloses a thermal shock gasifying electrospray ionization source and a mass spectrometry (MS) system, belonging to the technical field of ambient MS ionization. The MS ionization source disclosed by the invention comprises an electrospray generator, a heating device and a sampling device, wherein the heating device comprises a gasifying chamber and a heater providing heat for the gasifying chamber; a sample nozzle of a sample tube of the sampling device is configured in a way of facing the bottom of the gasifying chamber; the outlet end of a spraying capillary tube of the electrospray generator is opposite to the inlet of a mass spectrometer and is spaced from the inlet by a certain distance; the gasifying chamber and the sample nozzle are placed under the space between the outlet end of the spraying capillary tube and the inlet of the mass spectrometer. The ionization source provided by the invention gasifies a sample in a thermal shock manner and improves the heating efficiency of the sample through shortening heating time; since the sample is heated for a short time, heat energy is not converted into the molecular internal energy of the sample and only can be provided for the sample to overcome the intermolecular force, and the molecules of the sample cannot be decomposed due to heating; thus, the ionization source provided by the invention can be used for detecting heat-unstable and nonvolatile compounds and biological samples and solves the difficulty existing in the traditional thermal desorption technology.

The invention discloses a neutral desorption device and a neutral desorption extraction electrospray ionization mass spectrographic (ND-EESI-MS) analysis method. The neutral desorption device mainly comprises an air inlet tube and a sample tube which are diagonally arranged, and are fixed in a frame, an outlet of the air inlet tube is adjacent to an inlet of the sample tube and stretches out of the frame, wherein, an inlet of the air inlet tube is communicated with an air pump which conveys neutral gas to the air inlet tube, an outlet of the sample tube is communicated with a sample feed tubeof an EESI, the outer layer of the air inlet tube is equipped with a heating jacket, and can be further equipped with a liquid charging tube, and an outlet of the liquid charging tube is adjacent to the outlet of the air inlet tube and the inlet of the sample tube. The ND-EESI-MS analysis method is that the neutral desorption device collects samples and introduces sample flow into an extraction electrospray ionization source through the outlet of the sample tube for mass spectrographic analysis. The device and the method are especially applicable to rapid and real-time analysis of complex biological samples, food, medicines, environmental samples and the like, and also can perform in situ in vivo analysis on organisms.

An in-source atmospheric pressure electron capture dissociation (AP-ECD) method and apparatus for mass spectrometric analysis of peptides and proteins. An electrified sprayer generates a multiply-charged peptide/protein ions from a sample solution, a source of electrons for negative reagents, and a flow of gas for guiding positively charged ions from the electrified sprayer to a downstream reaction region within the guide. The reaction region being at or near atmospheric pressure and substantially free of the electric field from the electrified sprayer. In another embodiment, the method uses electron transfer dissociation (ETD), in the event that anions are substituted for electrons as the negative reagents. Fragment ions exiting the reaction region are subsequently passed into a mass analyzer of a mass spectrometer for mass analysis of the ions.

The invention discloses the field of an analytical instrument, in particular to an instrument for ion generation of medical micromolecules and biological macromolecules. The instrument is provided with a hollow capillary emission needle and a launch emission support. The emission needle support forms a laminar flow gas flowing forwards around the emission needle to eliminate the capillary reverseflow effect of liquid flowing out from the hollow capillary emission needle outside a capillary, so that the liquid flows forwards. An inlet infused in the capillary in a vacuum mode I specially designed into an arc-shaped mechanism, so the speed of airflow at some position at any direction is zero, the position is just the outlet of the hollow capillary emission needle, therefore the stability of a Taylor cone of the top end of the point of the emission needle is ensured in the large flow range, and finally a stable ion flow is obtained. The instrument has the advantages of stable ion emission, high ion transmission efficiency and the like and can be widely applied to occasions of ion source preparation.

The invention relates to a method of preparing a porous graphene film on the surface of a silicon wafer by using an electrostatic spray process combined with a heat treatment process of a substrate silicon wafer, and belongs to the technical field of solid film preparation processes. The method is characterized in that graphene dispersion liquid is prepared by a liquid phase ultrasound stripping method, and the graphene dispersion liquid is used as spinning liquid to prepare the porous graphene film on the surface of the substrate silicon wafer by the electrostatic spray process and a substrate heating method. The method is simple and convenient and has low requirements on equipment; and the prepared film has a porous structure, good adhesiveness with the substrate silicon wafer and a larger specific surface area, and is expected to be applied to the fields of gas sensitive sensors, lithium batteries, catalysts and the like.

The invention discloses a covalent organic framework material, a solid-phase microextraction probe and a preparation method and application thereof. The covalent organic framework material comprises 2, 3, 5, 6-tetrafluoro-p-dibenzaldehyde and a 2, 4, 6-tri(4-aminophenyl)1, 3, 5-triazine repetitive structure unit which take an imine bond as a connection mode. The solid-phase micro-extraction probeprepared from the material has high selectivity and high enrichment capacity on perfluoro and polyfluoroalkyl compounds, can be directly combined with a nanoliter electrospray ion source for mass spectrometry after sampling extraction, and has extremely high sensitivity, the method can be used for rapid, accurate and sensitive analysis of trace perfluoroalkyl and polyfluoroalkyl compounds in complex environments and biological samples.

The invention discloses an infrared-radiation-assisted high-voltage static spray device with a coaxial spinning nozzle, and a method for preparing polymer micro-nanometre particles with nuclear-shell structures by using the same. The device comprises an injector 1, an axial flow injection pump 1, an injector 2, an axial flow injection pump 2, a high-voltage power supply, a powder receiving plate and an infrared radiation source, wherein the coaxial spinning nozzle is formed on the injector 1 as well as the front end of an injection needle head of the injector 2 by a high-elasticity soft silicone hose; the high-voltage power supply is connected to the back end of the injection needle head of the injector 2 by a crocodile squeezer; the high-voltage power supply and the powder receiving plate are grounded together; and the infrared radiation source is arranged between a nozzle orifice of the coaxial spinning nozzle and the powder receiving plate. The method for preparing the polymer micro-nanometre particles with the nuclear-shell structures comprises two steps of solution preparing and spraying, and auxiliary drying through irradiation of the infrared radiation source. The preparation method disclosed by the invention is simple, and is suitable for the industrial production; and the obtained polymer nanometre particles with the nuclear-shell structures are fully dried, are small in diameter, and are controllable.

The invention relates to a method for detecting the cationic surfactants of alkyl quaternary ammonium salts with the liquid chromatography-tandem mass spectrometry and includes the following steps: pre-treating the detected samples with a Soxhlet extraction method or a supersonic extraction method; detecting with a liquid chromatograph-tandem mass spectrometer with the LC conditions of a C18 reverse-phase chromatographic column, a column temperature of 30 to 60 DEG C, the inlet volume of 3 to 10ul, the mobile phase flow rate of 0.15 to 0.60ml per minute; the tandem mass spectrometry MS/MS is provided with the conditions of ionizing by adopting the electro-spray ionization model and the detection mode of multi-ion reaction. The method adopted by the invention is characterized by accurate and fast analysis method, high method selectivity, little interference, etc.

The invention discloses a power-saving spraying device for an air conditioner, which comprises an outdoor unit provided with a condenser, a plurality of nozzles, and a water inlet control device, wherein the water inlet control device is arranged outside the outdoor unit, is respectively connected with a water source and the nozzles, and is used for controlling the spraying amount of the nozzles; the nozzles are arranged on the outside of at least one surface at the periphery of the outdoor unit, and the spraying direction of the nozzles faces the outdoor unit; and the water inlet control device controls water sprayed by the nozzles onto the condenser at any time to evaporate completely. The invention also provides an operating method for the power-saving spraying device; and the spraying is carried out after the cooling is started for a period of time, and more energy and power are saved. The power-saving spraying device for the air conditioner has the advantages of high heat exchange efficiency, low power consumption, simple structure and no maintenance.

The invention provides a method for detecting the quality of traditional Chinese medicine indigowoad root granules. The method comprises the following steps of: detecting the traditional Chinese medicine indigowoad root granules by using a soft-ionization mass spectrometry technique; performing primary evaluation on the number and strength of characteristic fingerprint peaks in an electro-spray fingerprint; measuring by high performance liquid chromatography to obtain the peak area of a chromatographic peak corresponding to each of cytidine, adenine, uridine, guanosine and adenosine serving as standard reference substances; and measuring according to a standard curve to obtain the cytidine content, adenine content, uridine content, guanosine content and adenosine content of one gram of the traditional Chinese medicine indigowoad root granules: 0.234 to 0.238 milligrams of cytidine, 0.116 to 0.118 milligrams of adenine, 0.472 to 0.474 milligrams of uridine, 0.331 to 0.334 milligrams of guanosine and 0.447 to 0.450 milligrams of adenosine. The method has the advantages of ensuring the scientificity and progressiveness of the method for detecting the quality of the traditional Chinese medicine indigowoad root granules, along with qualitative and quantitative performance.

The invention relates to an electro-spray device, and especially to a micro fluidic electro-spray chip device and a manufacture method. The chip device provides more uniform auxiliary airflow close to an electro-spray pin, a unique electrode structure ensures the electro-spray uniformity of the pin, and the problem of non-uniformity is overcome. The manufacture method can be used to conveniently manufacture the electro-spray chip device. The micro fluidic electro-spray chip device comprises fluid chips and gas chips, each fluid chip comprises a fluid transmission channel and the electro-spray pin, each gas chip comprises a gas transmission groove and a second electrode structure, and the fluid chip is bonded with the gas chip to form the micro fluidic electro-spray chip device. The manufacture method comprises the following steps that the gas chips and the fluid chips are processed, and the side, with the groove, of the gas chip is bonded with the side, with the electro-spray pin, of the fluid chip to form the micro fluidic electro-spray chip device, and during bonding, accurate alignment is realized by aligning marks on a silicon chip, so that the electro-spray pin on the fluid chip can be nested into a through hole of the gas chip.

The invention provides a single cell mass spectrometry method. The method comprises the following steps: carrying out cell immobilization and dilution treatment on cells to be detected so as to obtaina pretreated cell suspension liquid; injecting the pretreated cell suspension liquid into an inner cavity from a tail end of a capillary tube, wherein an electrode is inserted into the inner cavity;applying a voltage to the electrode so that the cells migrate to a capillary tip; completely evaporating the liquid in the capillary tube; dropwise adding an auxiliary liquid to the capillary tip, enabling the auxiliary liquid to enter the capillary tube to be in contact with the cells, extracting a target object; and meanwhile, applying the voltage to the electrode to perform electrospray so thatthe auxiliary liquid containing the target object is ionized, sprayed out from the tip and entering a sample inlet of a mass spectrometer to perform mass spectrometry. According to the method, sampling and mass spectrometry of the single cell can be achieved without depending on a high-precision control platform, identification of a double bond position of unsaturated lipids in the single cell can be achieved in combination with a photochemical derivation process, and an application prospect is good.

The invention relates to an analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS/MS), and belongs to an analysis and detection means for a renal function biomarker. The method is characterized in that the detection means is that an EESI source is subjected to direct MS and MS/MS under normal pressure, linear trap quadrupole (LTQ)-MS/MS is set to be in a positive ion detection mode, the parameters of the EESI source, such as the composition of an electrospray solvent are adjusted, an isotopic dilution technology and a standard addition method are adopted in quantitative analysis, creatinine at gradient concentration and deuterated creatinine at fixed concentration are added into an urine sample, direct analysis is performed, a standard addition curve is drawn, and the concentration of the urine creatinine is obtained. The urine creatinine can be directly measured under the condition that the sample is not pretreated, and the defects of a long analysis period and low efficiency in the prior art are overcome. The method has the characteristics of quickness, accuracy, capability of resisting interference from a substrate, and the like, and is particularly suitable for the clinical research of renal disease (such as the early diagnosis of acute renal failure) and high-flux sample analysis.

The present invention provides a high performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of specific migration of 18 phenols or phenol derivatives in five plastic food simulants. The method is as below: soaking a sample to be measured in food simulants, cooling to room temperature, mixing well, filtering a water-based soaking liquid by a PTFE syringe needle filter; t extracting vegetable food simulant with acetonitrile, and filtering the supernatant by a PTFE needle filter, and introducing sample; eluting and separating by a C8 column; and running under negative multiple reaction monitoring mode by using a mass spectrometry with electro-spray ionization source. The method has good chromatographic separation, linear relationship, high recovery and accuracy, meets limited requirements of 18 phenols or phenol derivatives in EU regulations NO 10/2011, and provides a quick and convenient method for the detection of the actual sample.