76 results about "Ionization mass" patented technology

The present invention provides a mass spectrometer having an ion lens capable of transporting an ion having a large mass to charge ratio with a high level of ion-passing efficiency even under a low-vacuum atmosphere. In conventional atmospheric pressure ionization mass spectrometers or similar mass spectrometers, applying an excessively high voltage to the ion lens undesirably causes an electric discharge. Therefore, the passing efficiency for an ion having a large mass to charge ratio cannot be adequately improved, which leads to a poor detection sensitivity. To solve this problem, the mass spectrometer according to the present invention includes a voltage controller 21 that controls a variable radiofrequency (RF) voltage generator 24 so that both the amplitude and the frequency of the RF voltage applied to the lens electrodes of an ion lens 5 are changed according to the mass to charge ratio of an ion to be analyzed. This control enables the ion lens 5 to focus an ion and transport it to the subsequent stage with a high level of passing efficiency even in the case of analyzing an ion having a large mass to charge ratio. Thus, the detection sensitivity is improved. The aforementioned control is conducted on the basis of the control data stored in a voltage control data storage 22. These data are obtained in advance by a measurement of a sample containing a substance having a known mass to charge ratio, in which the intensity of the signal of an ion detector is maintained while the analysis conditions are changed.

The invention relates to a novel electro-spray sampling vacuum ultraviolet single-photon ionization mass spectrometry analysis device which solves the analysis problems of a plurality of non-polarity composition complex systems. The device of the invention comprises a photoionization chamber and a differential chamber which are adjacent to each other; the adjacent side walls of the photoionization chamber and the differential chamber are communicated with a perforated ladle which is convex towards the differential chamber; the outlet of the perforated ladle is corresponding to the middle of the lead-out electrode and acceleration electrode of a time-of-flight mass spectrometer; the inlet of the perforated ladle is corresponding to an inverted horn-shaped nozzle on the side wall of the differential chamber; the inlet of the perforated ladle is in inverted horn shape; an electro-spray needle corresponding to the nozzle is arranged outside the differential chamber; the other end of the electro-spray needle is connected with an injection pump. The device of the invention combines the electro-spray sampling and vacuum ultraviolet photoionization mass spectrometry technique, directly sprays the sample to be measured out of the electro-spray needle during the testing and analysis process, and requires no complex pre-processing and separation of the sample; as the electro-spraying has no restrictions on the thermal stability and volatility of the sample, the device of the invention can be widely applied to the analysis and detection of simple organic matters and complex organic mixtures and can monitor certain organic reaction processes in real time.

The invention relates to a novel IR laser desorption / vacuum UV single-photon ionization mass spectrum analyzing device for analysis and detection of a biological sample, which solves the problems of the prior art that uses matrix and is unlikely to analyze complex mixture with low polarity and low molecular weight. The device is structurally characterized in that a photoionization chamber is arranged on one side of a time-of-flight mass spectrometer, an ion injection hole is provided on one sidewall of the photoionization chamber, an ion injector and a sample target are arranged in the chamber, a laser is disposed outside the photoionization chamber on the sample target side, and vacuum UV light source is arranged on the other side. By combining IR laser desorption technology and vacuum UV ionization mass spectrum technology, the invention can achieve high-efficiency and rapid analysis without selecting different matrixes according to the sample and without complex pre-treatment and separation of the sample, thus preventing the matrix interference on mass spectral signals. The invention is specifically suitable for analysis of thermal-unstable and nonvolatile organic compounds and biological molecules.

The present invention relates to a method capable of implementing on-target one-step desalination and enrichment of trace protein or polypeptide by means of combined application of hydrophobic polymer microblotting technique and matrix auxiliary laser analysis ionization mass spectrography. Said microblotting hydrophobic polymer has good adsorption and enrichment effect for protein and polypeptide, so that it can implement one-step desalination and direct MALDI-TOF-MS analysis. Said invention can be extensively used in the field of protein phylogeny.

Biological material is detected in a sample using a MALDI-MS technique (Matrix Assisted Laser Desorption and Ionization-Mass Spectroscopy). A liquid comprising the sample and a MALDI matrix material is prepared and used to form a continuous stream of the liquid. The stream is separated into successive parts to form drops, which are launched into flight, or the stream is launched into flight and then separated into drops. Drop forming techniques may be used that are known from ink jet printers. Material from the drops is ionized while in flight. Mass spectra from the ionized material of respective drops are measured. Preferably, before the drops are formed the liquid is diluted to a level where the majority of drops at most one micro-organism is present per drop.

Prior work on differential mobility analysis (DMA) combined with mass spectrometry (MS) has shown how to couple the output of the DMA with the inlet of an atmospheric pressure ionization mass spectrometer (APCI-MS). However, the conventional ion inlet to an APCI-MS is a round orifice, while conventional DMA geometries make use of elongated slits. The coupling of two systems with such different symmetries limits considerably the resolutions attainable by the DMA in a DMA-MS combination below the value of the DMA alone. The purpose of this invention is to overcome this limitation in the case of a parallel plate DMA. One solution involves use of an elongated rather than a circular MS sampling hole, with the long dimension of the MS inlet hole aligned with that of the DMA slit. Another involves use of a more elongated orifice in the DMA exit and a more circular hole on the MS inlet, the two being connected either through a short transfer conduit or through an ion guide. The DMAs described can also be coupled profitably to detectors and analyzers other than mass spectrometers.

Laser desorption / ionization time-of-flight mass spectrometer (“LDI-TOF-MS”) devices, and methods, that accurately measure the mass of analytes contained in a sample and which also measure the quantities of analytes present in a sample in a consistent manner from instrument-to-instrument and over time on a single instrument. In particular, the invention provides LDI-TOF-MS devices and methods in which: 1) the energy of the laser pulse and the area of the sample illuminated (fluence) is consistent and controlled so as to produce consistent conditions for analyte desorption and ionization; 2) the mass analyzer behaves in a reproducible manner; and 3) the detection system produces a signal that consistently represents the arrival of ions of different masses.

Prior work on differential mobility analysis (DMA) combined with mass spectrometry (MS) has shown how to couple the output of a planar DMA with the atmospheric pressure inlet of an atmospheric pressure ionization mass spectrometer (APCI-MS). However, because the ion inlet to APCI-MS instruments is a round orifice, while conventional DMA geometries make use of elongated slits, the coupling of both has attained less resolving power or tolerated a smaller sample flow rate than a DMA alone. The present invention overcomes these limitations with an axial DMA of cylindrical symmetry using more than two electrodes. The configuration is related to that previously proposed by Labowsky and Fernández de la. Mora (2004, 2006), where ions with a critical electrical mobility are brought into the symmetry axis of the DMA. Ions with this critical mobility are now optimally transmitted into the MS, with much higher resolution than possible in planar DMAs. In a preferred embodiment of this DMA facilitating DMA-MS coupling, one DMA electrode intersecting the symmetry axis is relatively planar.

The invention provides an ultra-sonic jet multi-photon resonance ionization type mass analyzing device.The laser beam ionization mass analyzing device includes a pulsed gas ejecting device 12 for ejecting in pulse mode carrier gas containing sample molecules into a vacuum vessel 17, a laser beam irradiation system for irradiating laser beam for selective photo-reaction of sample molecules in said pulsed gas, repeller and extraction electrodes 18 and 19 generating an electric field for extraction of sample molecular ions generated by the photo reaction and a mass analyzing device 26 for mass analysis of extracted sample molecular ions. The laser beam irradiation system is set to irradiate laser beam to sample molecules near a position whereat a pressure time distribution of pulsed gas translating in the vacuum vessel 17 transitions from a flat-top pressure distribution to a triangular pressure distribution.

A laser sputtering ionization cold focusing orthogonal time-of-flight mass spectrometer relates to a mass spectrometer. Provided is a laser sputtering ionization cold-focus orthogonal time-of-flight mass spectrometer that matches a laser ionization source with a mass spectrometer, removes interference peaks, provides clear spectrograms, and analyzes solid samples without a standard sample. A laser, a focusing lens group, a sampling probe rod, a two-dimensional mobile platform, an ion source cavity, a main cavity, a sampling cone, a radio frequency multipole rod, a skimmer cone, an ion lens group and a time-of-flight mass analyzer are provided. The laser and focusing lens are set outside the ion source cavity, and the sample injection probe, two-dimensional mobile platform and sampling cone are set inside the ion source cavity; the wall of the ion source cavity is equipped with quartz windows, vacuum ports and valves; the main cavity A vacuum port is set on the wall of the body cavity, and the skimmer, radio frequency multi-stage rod and ion lens group are arranged in the main cavity; the sampling probe rod, two-dimensional mobile platform and sampling cone are the same as the skimmer cone, radio frequency multi-stage rod and ion lens group. axis.

The invention provides a microwave plasma generator for a proton transfer mass spectrometer, comprising a microwave generator, and a microwave cavity, an end cover, a tuning rod and a microwave coupling adjustment module which are made of metal materials, as well as a discharge tube made of an insulating material. The invention also provides a microwave plasma source-based mass transfer ionization mass spectrometer which comprises a steam supply system for providing water vapor, a microwave plasma generator leading the water vapor to generate H3O<+> ions, a drift tube for a proton transfer reaction of analyte molecules and H3O<+> ions, a mass spectrometer for analysis and detection, and a vacuum regulation system, wherein the steam conveying pipe of the steam supply system is connected with the discharge pipe of the plasma generator; the discharge pipe of the microwave plasma generator is connected with the drift tube through a first joint; and a sample inlet formed on the drift tube is connected with the mass spectrometer through a second joint.

In order to provide an atmospheric pressure ionization mass spectrometer system which allows for equal high sensitivity analysis for LCs with different flow rates, there is provided an atmospheric pressure ionization mass spectrometer system comprising: an atmospheric pressure ion source for ionizing a sample solution under atmospheric pressure, a mass spectrometer for mass analyzing the ions in an evacuated space, a fine hollow tube on a partition wall between the atmospheric pressure ion source and the mass spectrometer, the ions generated in the atmospheric pressure ion source being introduced through the fine tube into the mass spectrometer to be mass analyzed, wherein the fine tube consists of a first fine tube and a second fine tube which are different in diameter, the second fine tube being inserted in the first fine tube, the ions and gas generated in the atmospheric pressure ion source are introduced into the mass spectrometer through the second fine tube, and a gas is fed into a space between the first fine tube and the second fine tube. The present invention allows for high sensitivity measurements of mass spectrometer systems including the micro LC, CE, and nanospray with very low flow rate and the conventional LC with much higher flow rate. In addition, the clogged fine tube can be exchanged without stopping the vacuum pumping, providing the simplified maintenance.

Disclosed in the invention is a femtosecond laser post-ionization mass spectrum apparatus comprising a vacuum chamber, a sample fixed bracket, a primary ionization source, a post-ionization source, an ion optics system and a flight time mass spectrum unit. An ion gun or laser is used as the primary ionization source to carry out sputtering on the solid surface; and during the primary ionization source sputtering process, a sample ion and a neutral particle can be generated, wherein the neutral-particle-based sputtering part enables the accuracy and the resolution ratio of the analysis result to be limited greatly. According to the invention, the femtosecond laser is used as the post-ionization means of the neutral particle, so that the ionization efficiency of the sample is substantially improved. And the sample ionization component is analyzed by using the flight time mass spectrum way.

Laser desorption / ionization time-of-flight mass spectrometer (“LDI-TOF-MS”) devices, and methods, that accurately measure the mass of analytes contained in a sample and which also measure the quantities of analytes present in a sample in a consistent manner from instrument-to-instrument and over time on a single instrument. In particular, the invention provides LDI-TOF-MS devices and methods in which: 1) the energy of the laser pulse and the area of the sample illuminated (fluence) is consistent and controlled so as to produce consistent conditions for analyte desorption and ionization; 2) the mass analyzer behaves in a reproducible manner; and 3) the detection system produces a signal that consistently represents the arrival of ions of different masses.

A laser optical bench for use with a laser desorption / ionization mass spectrometer. The laser optical bench includes a laser for producing light, a focusing structure that receives light from the laser and focuses predominantly in a single plane, an attenuator that receives light from the focusing structure, beam steering structure for directing light from the attenuator from the target; and a final focusing element for focusing light from the beam steering structure on the target. Further focusing elements may be included for further focusing and dispersing the light beam in different planes. Additionally, photodetectors or photodiodes may be included for energy measurement and sensing a lasing event.

The invention provides an automatic sampling device for neutral desorption and an ionization mass spectrum analyzing method. The device is of a box structure with an inlet end and an outlet end and comprises a rotatable workbench, a sampling needle, a clamping mechanism and a motor, wherein the rotatable workbench is arranged in a cavity of the box, the sampling needle is hung above the workbench and can vertically move, the clamping mechanism is used for clamping the sampling needle, and the motor is used for driving the workbench and the sampling needle to move. According to the method, quick and batched neutral-desorption electrospraying extraction ionization mass spectrum analyzing on thick liquid samples is achieved by adopting a neutral-desorption electrospraying extraction ionization device comprising the automatic sampling device to be matched with a mass spectrum scanner. Therefore, by adopting the automatic sampling device, quick and batched mass spectrum analyzing on thick liquid can be achieved without any preprocessing under the normal pressure; meanwhile, due to the fact that the neutral desorption device is in a closed environment, the outside influence can be excluded as much as possible; the device can perform automatic sampling and sample replacing under the control of a circuit board and a motor and is simple in structure, convenient to use and capable of saving the human cost.

The invention provides an ultra-sonic jet multi-photon resonance ionization type mass analyzing device. The laser beam ionization mass analyzing device includes a pulsed gas ejecting device 12 for ejecting in pulse mode carrier gas containing sample molecules into a vacuum vessel 17, a laser beam irradiation system for irradiating laser beam for selective photo-reaction of sample molecules in said pulsed gas, repeller and extraction electrodes 18 and 19 generating an electric field for extraction of sample molecular ions generated by the photo reaction and a mass analyzing device 26 for mass analysis of extracted sample molecular ions. The laser beam irradiation system is set to irradiate laser beam to sample molecules near a position whereat a pressure time distribution of pulsed gas translating in the vacuum vessel 17 transitions from a flat-top pressure distribution to a triangular pressure distribution.

The invention provides application of a hexagonal boron nitride nanosheet in preparing a matrix solution and a mass spectrometric detection method, and particularly provides application of a hexagonal boron nitride naonsheet in preparing a matrix solution. The matrix solution is used for matrix assistant laser desorption / ionization mass spectrometric detection. Therefore, a matrix can be prevented from generating background peaks in the analysis process not to interfere the detection of a sample, so that the accuracy of the mass spectrometric detection can be improved.

Prior work on differential mobility analysis (DMA) combined with mass spectrometry (MS) has shown how to couple the output of the DMA with the inlet of an atmospheric pressure ionization mass spectrometer (APCI-MS). However, the conventional ion inlet to an APCI-MS is a round orifice, while conventional DMA geometries make use of elongated slits. The coupling of two systems with such different symmetries limits considerably the resolutions attainable by the DMA in a DMA-MS combination below the value of the DMA alone. The purpose of this invention is to overcome this limitation in the case of a parallel plate DMA. One solution involves use of an elongated rather than a circular MS sampling hole, with the long dimension of the MS inlet hole aligned with that of the DMA slit. Another involves use of a more elongated orifice in the DMA exit and a more circular hole on the MS inlet, the two being connected either through a short transfer conduit or through an ion guide. The DMAs described can also be coupled profitably to detectors and analyzers other than mass spectrometers.

The present invention is devoted to an analytical method for directly determining carbon number and molecular weight distributions of isoparaffin mixtures. The analytical means uses low in-scan emitter-current field-ionization mass spectroscopy to substantially reduced or eliminated molecular ion fragmentation for isoparaffins, which allows direct measurement of isoparaffin molecular ions for their distributions. The analytical means is capable of directly characterizing isobutane to C50+ isoparaffins, covering naphtha to gas oil ranges.

Based on first dimension chromatographic separation, the system applies multichannel chromatogram array to second chromatographic separation so that analysis efficiency is raised greatly. Multichannel chromatogram array is possible to expand to 2-50 channels. Multichannels are interfaced to first dimension chromatogram to complete separation procedure of enrichment and gradient elution from one column to another, providing effective measure for analyzing samples of protein group. Comparing with method of single channel one or two dimensions chromatogram, the invented method shortens analytical cycle, provides easy operation. Integrated assembles in the invention can be utilized in combined with laser ionization mass spectrum so as to realize automatic chromatograph-mass spectrograph for protein group.

An atmospheric pressure ionization mass spectrometry system has an atmospheric pressure ionization chamber for ionizing a sample, an evacuated intermediate evacuation chamber into which generated ions are introduced through a capillary tube, and a vacuum chamber further downstream therefrom into which ions are introduced for mass separation. A partition wall separating the atmospheric pressure ionization chamber from the intermediate evacuation chamber includes a small orifice having a diameter corresponding to an internal channel diameter of the capillary tube. The capillary tube is detachably installed on the partition wall so that an outlet end of the capillary tube abuts on the small orifice. The internal channel of the capillary tube is in communication with the small orifice. The capillary tube can be installed and removed from the system without breaching the vacuum.

This invention relates to a nanowire-assisted method for mass spectrometric analysis of a specimen. More specifically, by using nanowire which can fix a specimen and perform desorption / ionization of the specimen while effectively transferring laser energy to the specimen to be irradiated, thereby enabling to perform mass spectrometric analysis without using a matrix solution. This invention, by effectively performing desorption / ionization of a specimen using the above-mentioned nanowire, enables to effectively perform qualitative-, quantitative-, and micro-analyses of specimens as well as low molecular weighted specimens. Further, this invention enables to the typical device of mass spectrometric analysis used in MALDI-T of MS. In particular, this invention can perform mass spectrometric analysis of a specimen with molecular weight of less than 1,000 Da and perform quantitative analysis by fixing a specimen with a predetermined area.

The invention discloses a method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum. The method comprises the following steps: A, extracting euphausia superb oil through alcohol; B, ionizing the euphausia superb oil, then introducing a mass spectrometer and performing detection to obtain a mass spectrum profile map; and C, comparing the mass spectrum profile map through MassLynx, and then determining a lipidomic profile of samples. The method is capable of achieving real-time mass spectrum detection without the need of carrying out pretreatment on the samples, is applicable to lipidomic detection of foods, and is capable of accelerating the development of shrimp oil which takes euphausia superb as a raw material.

Biological material is detected in a sample using a MALDI-MS technique (Matrix Assisted Laser Desorption and Ionization-Mass Spectroscopy). A liquid comprising the sample and a MALDI matrix material is prepared and used to form a continuous stream of the liquid. The stream is separated into successive parts to form drops, which are launched into flight, or the stream is launched into flight and then separated into drops. Drop forming techniques may be used that are known from ink jet printers. Material from the drops is ionized while in flight. Mass spectra from the ionized material of respective drops are measured. Preferably, before the drops are formed the liquid is diluted to a level where the majority of drops at most one micro-organism is present per drop.

The invention provides a quality detection method of a traditional Chinese medicine of isatis root. The quality detection method comprises the following steps of: after processing the traditional Chinese medicine of the isatis root by sample pre-processing methods, such as extracting with water, processing with ethanol, sequentially leaching, concentrating, determining the volume with ethyl acetate and water saturated n-butyl alcohol, and the like, obtaining the fingerprint of the traditional Chinese medicine of the isatis root by electro-spray-ionization mass spectrum; measuring the content of purine and nucleosides by utilizing a high performance liquid chromatography (HPLC); and integrating the fingerprint and the measured data on the content to evaluate the quality of the traditional Chinese medicine of the isatis root. The quality detection method is accurate, rapid, convenient and effective.

Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and / or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.

Inside a first-stage intermediate vacuum chamber (6) of an atmospheric-pressure ionization mass-spectrograph apparatus, cluster ions that are the cause of background noise are generated mainly at area (A), and fragment ions are generated mainly at area (B). Accordingly, in cases of in-source CID analysis mode, a DC voltage that is lower than that of a skimmer (8) is applied to a first ion guide (7), so that an accelerating electric field is generated at area (B). This enables sufficient energy to be given to the ions, to facilitate fragmentation. In cases when in-Source CID analysis is not conducted, a DC voltage that is lower than that of the first ion guide (7) is applied to the exit end (3b) of a desolvation tube (3), so that no electric field is generated at area (B) and an accelerating electric field is generated only at area (A). This enables inhibition of generation of both the cluster ions and the fragment ions, and attainment of a high-quality chromatogram.

The invention belongs to the technical field of radiation monitoring, and relates to a preparation method of a plutonium source for a laser resonance ionization mass spectrometric test. The preparation method comprises the following steps of electrodeposition: electrodepositing plutonium oxide on a rhenium ribbon; vacuum titanium evaporation: evaporating metallic titanium on the rhenium ribbon on which the plutonium oxide is electrodeposited. By utilizing the preparation method of the plutonium source for the laser resonance ionization mass spectrometric test, which is provided by the invention, the loss of plutonium in the preparation process of the plutonium source can be reduced; the generation of difficult-to-decompose Pu compounds of PuC and the like is prevented; the atomization efficiency of the plutonium is improved; the formation of a plutonium ion is prevented.