Nanowire Assisted Laser Desorption/Ionization Mass Spectrometric Analysis

a mass spectrometric analysis and laser desorption technology, applied in the direction of instruments, particle separator tube details, separation processes, etc., can solve the problems of difficult to select a suitable matrix substance for a given specimen, unable to perform an anlaysis for an unknown substance in a mixture, and its use is largely limited

Inactive Publication Date: 2008-10-09
KOREA INST OF SCI & TECH
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Problems solved by technology

However, the exact mechanism of the ionization is yet to be elucidated and therefore it is challenging to select a suitable matrix substance for a given specimen.
Besides, MALDI has a disadvantage that its use is largely limited to substances having a molecular weight greater than 1,000 Da because low molecular weighted matrix substance and matrix decomposed product are indicated on the mass spectrometry spectrum in the course of ionization of a specimen by a laser-activated matrix.
In addition, the ionization of a decomposed product is determined according to the selection of a matrix and thus it becomes necessary to select a most suitable matrix substance and it becomes difficult to perform an anlaysis for an unknown substance in a mixture (G. Suizdak, 1.
Further, MALDI method has another disadvantage that the spatial distribution of a crystallized specimen obtained via specimen preparation process is not uniform and therefore the amount of a specimen being excited by laser varies depending on the location of irradiation.
However, even with the above method, it is still difficult to perform an accurate quantitative analysis of a specimen via MALDI method.
Meanwhile, in case of DIOS, a specimen solution penetrates the porous structure of a board and becomes crystallized in the course of injecting a specimen solution on the porous silicon board thus making it difficult to limit the size of crystals and also hard to make the crystal center of a specimen correspond to a point of laser irradiation.
Since DIOS is only applicable to silicon, it cannot select an energy transfer medium suitable for various kinds of specimens.
Further, an effective desorption / ionization becomes difficult because the laser energy can be transferred via a two-dimensional way.

Method used

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  • Nanowire Assisted Laser Desorption/Ionization Mass Spectrometric Analysis

Examples

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example 1

Mass Spectrometric Analysis using Nanowires

[0086]A surface of a silicon board was deposited with Au via sputtering vapor deposition method to a thickness of 2 nm, wherein the area for deposition was made circular with a diameter of 200 μm using a mask. Then, the board was supplied with SiCl4 at 500° C. via chemical vapor deposition (CVD) method, thereby allowing the growth of silicon nanowires, and FIG. 1 shows the silicon nanowires grown on the board. The silicon nanowires used were restricted to have a length of 5 μm, a diameter of 100 nm and a density of about 1.8×106 NWs / mm2. The board with thus grown nanowires was inserted into a specimen inspection plate of a mass spectrometric analysis device. The mass analysis was performed using Reflex 3 from Bruker Daltonics (Germany). The specimens used were two peptides, angiotensin and leucine enkephalin. The diameter of nanowire spot was restricted to be 0.2 mm and the crystals of specimens were irradiated by the circular spot of laser...

example 2

Mass Spectrometric Analysis using Nanowire Suspension

[0087]A surface of a silicon board was deposited with Au via sputtering vapor deposition method to a thickness of 2 nm, wherein InN nanowires were grown by supplying with InCl3 and NH3 at 500° C. via chemical vapor deposition (CVD) method. Nanowire suspension was prepared by separating nanowires from the board by placing the above silicon board in isopropyl alcohol and then applying with ultrasonic wave for 10 sec. The MALDI measurement was performed using the nanowire suspension by using MALDI target from Bruker Daltonics (Germany) instead of the typical matrix. Taking advantage of the special property of the MALDI target that crystal center of a specimen is concentrated on ankers, a small amount of nanowire suspension was treated to form nanowire islets around the ankers. Then, peptides as a specimen was dropped in the above nanowire islets and allowed to form crystals of the specimen. Upon inspection, it was found that nanowire...

example 3

Detection of Coupling Reaction using Nanowires

[0088]A nanowire target where nanowires are grown as in Example 1 was added with hepatitis B antigen solution and allowed to incubate at a 37° C. water-saturated thermostat for an hour so that the hepatitis B antigen can be fixed. Then, the nanowire target was dipped into a washing solution to separate unfixed hepatitis B antigens. A specimen solution containing the hepatitis B antigen was added to the nanowires to induce an antibody-antigen reaction and then placed the nanowire target into the washing solution to remove the specimen which was left after the antibody-antigen reaction. Then, it was confirmed that MALDI enables to perform quantitative as well as qualitative analyses of antigen-antibody coupler which was coupled to nanowires. Likewise, it is possible to quantitatively detect the couplers between DNAs, between DNA and RNA or between proteins using the nanowire MALDI target. Further, it is also possible to quantitatively dete...

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Abstract

This invention relates to a nanowire-assisted method for mass spectrometric analysis of a specimen. More specifically, by using nanowire which can fix a specimen and perform desorption/ionization of the specimen while effectively transferring laser energy to the specimen to be irradiated, thereby enabling to perform mass spectrometric analysis without using a matrix solution. This invention, by effectively performing desorption/ionization of a specimen using the above-mentioned nanowire, enables to effectively perform qualitative-, quantitative-, and micro-analyses of specimens as well as low molecular weighted specimens. Further, this invention enables to the typical device of mass spectrometric analysis used in MALDI-T of MS. In particular, this invention can perform mass spectrometric analysis of a specimen with molecular weight of less than 1,000 Da and perform quantitative analysis by fixing a specimen with a predetermined area.

Description

TECHNICAL FIELD[0001]This invention relates to a nanowire-assisted method for mass spectrometric analysis of a specimen. More specifically, this invention, by using nanowire, can fix a specimen and perform desorption / ionization of the specimen while effectively transferring laser energy to the specimen to be irradiated, thereby performing mass spectrometric analysis without using a matrix solution.BACKGROUND OF INVENTION[0002]Mass spectrometer is an analytical device for measuring a mass of a compound. It generally determines molecular weight of a compound by measuring the value of mass-to-charge (m / z) by ionizing the compound by charging. There are many methods to ionize a compound such as electron ionization using electron beams, high speed collision of atoms, a method using laser, and a method to spray a specimen into an electric field.[0003]For a biochemical substance with an extremely large mass such as proteins and nucleic acids, MALDI-Tof Mass Spectroscopy (Matrix Assisted La...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01D59/44H01J49/16G01N27/62
CPCH01J49/0418B82B1/00G01N27/623G01N27/64
Inventor CHOI, HEON JINPYUN, JAE CHUL
Owner KOREA INST OF SCI & TECH
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