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Automatic sampling device for neutral desorption and ionization mass spectrum analyzing method

A neutral desorption and automatic sampling technology, applied in the field of pharmaceutical and chemical analysis, can solve the problems of limited number of samples, slow analysis speed, poor stability, etc., to save labor costs, improve analysis speed, and expand the scope of application.

Active Publication Date: 2016-05-11
江西恒烑科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the above-mentioned prior art, ND-EESI devices are mostly built by hand, especially the sampling devices are generally exposed to the air, and there are deficiencies such as poor air tightness, limited number of analyzed samples, poor stability, and slow analysis speed. In order to further ensure the ND-EESI The reliability of EESI-MS mass spectrometry analysis is based on the ionization mechanism of ND-EESI, and it is especially important to design a commercially available and highly stable sampling device for ND-EESI

Method used

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  • Automatic sampling device for neutral desorption and ionization mass spectrum analyzing method
  • Automatic sampling device for neutral desorption and ionization mass spectrum analyzing method
  • Automatic sampling device for neutral desorption and ionization mass spectrum analyzing method

Examples

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Effect test

Embodiment 1

[0053] The mass spectrometric analysis of dipalmitophosphatidylcholine (DPPC) standard substance was carried out by the above-mentioned ND-EESI-MS method. The results of the analysis can be found in image 3 .

[0054] In this example, if image 3 As shown, using the above method, set the scanning range of the mass spectrometer to 50 to 2000Da, and the phospholipids are mainly located in the range of 700 to 900Da, so the target substance is amplified and analyzed, and it is found that m / z757 can be detected, and through multi-stage It was identified by tandem mass spectrometry and proved to be DPPC.

Embodiment 2

[0056] In this embodiment, the device and method of the present invention are used to sample and analyze the sputum of normal people and the sputum of lung cancer patients respectively.

[0057] Atmospheric pressure mass spectrometry method is used to quickly determine the sputum fingerprints of lung cancer patients and normal people. In the physical examination, the sputum is detected to determine the possibility of lung cancer. If the sputum is the same as the fingerprint of lung cancer patients or Similarly, high-resolution CT and other examinations are further carried out to confirm the diagnosis, so as to achieve a quick diagnosis or exclude the possibility of lung cancer, which is of great significance for the rapid diagnosis or exclusion of lung cancer.

[0058] In this embodiment, after the sputum is collected with the sample collection bottle 132, according to the method of the present invention, a mixed solution of methanol, water and acetic acid (volume ratio 50:48:2...

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Abstract

The invention provides an automatic sampling device for neutral desorption and an ionization mass spectrum analyzing method. The device is of a box structure with an inlet end and an outlet end and comprises a rotatable workbench, a sampling needle, a clamping mechanism and a motor, wherein the rotatable workbench is arranged in a cavity of the box, the sampling needle is hung above the workbench and can vertically move, the clamping mechanism is used for clamping the sampling needle, and the motor is used for driving the workbench and the sampling needle to move. According to the method, quick and batched neutral-desorption electrospraying extraction ionization mass spectrum analyzing on thick liquid samples is achieved by adopting a neutral-desorption electrospraying extraction ionization device comprising the automatic sampling device to be matched with a mass spectrum scanner. Therefore, by adopting the automatic sampling device, quick and batched mass spectrum analyzing on thick liquid can be achieved without any preprocessing under the normal pressure; meanwhile, due to the fact that the neutral desorption device is in a closed environment, the outside influence can be excluded as much as possible; the device can perform automatic sampling and sample replacing under the control of a circuit board and a motor and is simple in structure, convenient to use and capable of saving the human cost.

Description

technical field [0001] The invention belongs to the fields of medicine and chemical analysis, and relates to a sampling device for liquid desorption in mass spectrometry, in particular to an automatic sampling device for neutral desorption of viscous liquid and an ionization mass spectrometry method. Background technique [0002] Lung cancer is a disease with high morbidity and mortality. Sputum examination is currently the only specimen for non-invasive diagnosis of non-small cell lung cancer (NSCLC). Sputum specimens have the advantages of non-invasiveness, easy collection, storage and transportation. What is more important is that sputum is the physiological covering of the tracheal mucosa, which can more objectively reflect the pathophysiological changes of the bronchi. In addition to the traditional sputum cytology examination, modern sputum detection methods include sputum-based cytology, sputum automatic / semi-automatic DNA image analyzer, sputum molecular marker exami...

Claims

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Application Information

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IPC IPC(8): G01N1/14G01N30/06G01N30/72
CPCG01N1/14G01N30/06G01N30/7266
Inventor 魏益平陈焕文刘坤徐建军张建勇丁健桦熊剑文
Owner 江西恒烑科技有限公司
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