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High flux protein multidemension array chromatogram separating system

A chromatographic separation, high-throughput technology, applied in material separation, measurement devices, analytical materials, etc., can solve problems such as analytical throughput limitation and analysis time extension, and achieve improved analytical throughput, improved analytical throughput, and separation efficiency. improved effect

Inactive Publication Date: 2005-01-12
FUDAN UNIV
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Problems solved by technology

Although the two-dimensional chromatography system has been commercialized last year, it still has great limitations in the analysis throughput. A sample needs multiple elution cycles (multi-salinity gradient and reversed-phase gradient), which makes The analysis time is greatly extended. Generally, it takes 2-3 days to analyze a proteome sample that has been simplified by pre-fractionation.

Method used

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  • High flux protein multidemension array chromatogram separating system
  • High flux protein multidemension array chromatogram separating system
  • High flux protein multidemension array chromatogram separating system

Examples

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Embodiment Construction

[0027] 1. Establishment of high-throughput protein multidimensional array chromatography separation system

[0028] 1. Production of multi-channel chromatographic interface:

[0029] Take a polyetheretherketone (PEEK) capillary tube with a length of 5 cm and an inner diameter of 500 μm, drill 30 small holes with a diameter of 380 μm on one side of the PEEK capillary with a drill bit of 380 μm, and the distance between the holes is 1 mm, and wash away the inside of the PEEK tube. debris and blow dry with nitrogen. A quartz capillary with an outer diameter of 480 μm was inserted along the PEEK capillary, and 30 quartz capillaries (75 μm i.d.; 380 μm o.d.) with a length of 5 cm and an outer diameter of 380 μm were inserted into the small holes to offset the built-in capillary, and the PEEK central channel was extracted. The built-in capillary is then glued around the intersection of the quartz capillary and the PEEK capillary, such as image 3 .

[0030] 2. Fabrication of mult...

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Abstract

Based on first dimension chromatographic separation, the system applies multichannel chromatogram array to second chromatographic separation so that analysis efficiency is raised greatly. Multichannel chromatogram array is possible to expand to 2-50 channels. Multichannels are interfaced to first dimension chromatogram to complete separation procedure of enrichment and gradient elution from one column to another, providing effective measure for analyzing samples of protein group. Comparing with method of single channel one or two dimensions chromatogram, the invented method shortens analytical cycle, provides easy operation. Integrated assembles in the invention can be utilized in combined with laser ionization mass spectrum so as to realize automatic chromatograph-mass spectrograph for protein group.

Description

technical field [0001] The invention is a high-throughput protein multidimensional array chromatographic separation system and a separation method using the system. Background technique [0002] Proteomics is one of the hottest areas of scientific research at present. The essence of its research is the large-scale separation and analysis of proteins at the cellular level. Unlike the static genome, proteins have complex post-translational modifications, and their expression in cells is diverse and dynamic. Due to the high complexity of proteomic samples, the rise and development of proteomics pose new demands and challenges to existing analysis techniques. The development of high-throughput and high-separation technology platforms has become the bottleneck of current proteomics research. [0003] At present, the core technology of proteome research in the world is two-dimensional gel electrophoresis-mass spectrometry. Although two-dimensional gel electrophoresis is the mo...

Claims

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Application Information

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IPC IPC(8): G01N30/46
Inventor 张祥民刘春丽
Owner FUDAN UNIV
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