Quality detection method of traditional Chinese medicine of isatis root

A quality detection method, the technology of Radix Radix, applied in the field of natural medicinal chemistry, can solve the problem of not being able to control the quality of Radix Radix as a whole

Active Publication Date: 2010-06-02
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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Problems solved by technology

[0002] Banlangen (RADIXISATIDIS) is the dried root of Isatis indigotica Fort., a cruciferous plant. It has the effects of clearing heat and detoxifying, cooling blood and relieving throat. Throat dansha, big head plague, erysipelas, carbuncle [1] , Modern pharmacodynamic studies have shown that Banlangen has pharmacological effects such as antibacterial, antiviral, antiendotoxin, immune enhancement and antitumor [2-6] , at present, the quality control method of Radix isatidis is mainly based on the quantitative analysis of components such as indigo and indirubin [7，8] , not enough to control the quality of Banlangen as a whole, as a common clinical traditional Chinese medicine, its authenticity is affected by many factors such as its place of origin, harvest time, and drying conditions. Therefore, it is necessary to establish a scientific quality detection method Evaluate the quality of Radix Radix, (References: [1] Pharmacopoeia Committee of the People's Republic of China. Pharmacopoeia of the People's Republic of China [M]. Beijing: Chemical Industry Press, 2005: 142-143. [2] Zhao Yanling, Cao Lin, Wang Jiabo , et al. Cluster analysis and antibacterial activity correlation analysis of fingerprints of n-butanol parts of Radix isatidis [J]. Experimental study on the relationship between lectin potency and inhibition of cold virus [J]. Journal of Shanghai University of Traditional Chinese Medicine, 2001, 15(3): 238-240. [4] Hou Qi, Cheng Guifang, Zhang Chengyi, et al. Four kinds of stimulants and the effect of anti-inflammatory drugs on the production of IL-8 by HL-60 cells[J]. Acta Pharmaceutica Sinica, 2000, 35(3): 173-176. [5] Xu Yimin, Lu Pingcheng, Wang Yongzhen, etc. Isatidis polysaccharide promotes immune function Experimental research on [J]. Journal of Integrated Traditional Chinese and Western Medicine, 1991, 11(6): 357-359. [6] Liang Yonghong, Hou Huaxin, Li Danrong, etc. Study on anticancer activity of isatidis dione B in vitro [J]. Chinese herbal medicine, 2000, 31(7): 531-533. [7] Luo Weiwei, He Yingju, Wang Ling, etc. Determination of the content of indigo and indirubin in Radix isatidis extract by HPLC [J]. West China Pharmaceutical Journal, 2004, 19(6) : 455-456. [8] Wang Banglin, Wang Qiang. HPLC Analysis of Indirubin and Indigo in Isatidis and Folium Isatidis [J]. Tianjin Pharmacy, 1994, 69(3): 30-33.)

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  • Quality detection method of traditional Chinese medicine of isatis root

Examples

Experimental program
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Effect test

Embodiment 1

[0017] (1) Preparation of test sample solution

[0018] Take Banlangen as raw material, weigh 2g of Banlangen herbal decoction pieces, add 40mL water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, filter, and concentrate under reduced pressure And dry to obtain the Radix Radix Radix water extract, add 25mL water to dissolve the Radix Radix Radix Radix obtained water extract, add 25mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, concentrate under reduced pressure and dry, the residue is 50% ( V / V) Methanol is dissolved and fixed to 10mL and shaken up, which is the test solution 1; the part after extraction is extracted 3 times by adding 25mL water-saturated n-butanol, and the water-saturated n-butanol extract is combined and concentrated under reduced pressure And dry, residue is dissolved with 50% (V / V) methanol and is settled to 10mL, is need t...

Embodiment 2

[0026] (1) Preparation of test sample solution

[0027] Take Banlangen as raw material, weigh 4g of Banlangen herbal decoction pieces, add 80mL of water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, filter, and concentrate under reduced pressure And dry to obtain the Radix Radix Radix water extract, add 50mL water to dissolve the Radix Radix Radix Radix obtained water extract, add 50mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, concentrate under reduced pressure and dry, the residue is 50% ( V / V) Methanol is dissolved and fixed to 10mL and shaken, which is the test solution 1; the part after extraction is extracted 3 times by adding 50mL water-saturated n-butanol, and the water-saturated n-butanol extract is combined and concentrated under reduced pressure And dry, residue is dissolved with 50% (V / V) methanol and is settled to 10mL, is need t...

Embodiment 3

[0035] (1) Preparation of test sample solution

[0036] Take Radix isatidis as raw material, weigh 6g of Radix isatidis medicinal herbal pieces, add 120mL water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, filter, and concentrate under reduced pressure And dry to obtain the Radix Radix Radix water extract, add 50mL water to dissolve the Radix Radix Radix Radix obtained water extract, add 50mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, concentrate under reduced pressure and dry, the residue is 50% ( V / V) Methanol is dissolved and fixed to 10mL and shaken, which is the test solution 1; the part after extraction is extracted 3 times by adding 50mL water-saturated n-butanol, and the water-saturated n-butanol extract is combined and concentrated under reduced pressure And dry, residue is dissolved with 50% (V / V) methanol and is settled to 10mL, i...

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Abstract

The invention provides a quality detection method of a traditional Chinese medicine of isatis root. The quality detection method comprises the following steps of: after processing the traditional Chinese medicine of the isatis root by sample pre-processing methods, such as extracting with water, processing with ethanol, sequentially leaching, concentrating, determining the volume with ethyl acetate and water saturated n-butyl alcohol, and the like, obtaining the fingerprint of the traditional Chinese medicine of the isatis root by electro-spray-ionization mass spectrum; measuring the content of purine and nucleosides by utilizing a high performance liquid chromatography (HPLC); and integrating the fingerprint and the measured data on the content to evaluate the quality of the traditional Chinese medicine of the isatis root. The quality detection method is accurate, rapid, convenient and effective.

Description

technical field [0001] The invention belongs to the field of natural medicine chemistry, and in particular relates to a quality detection method of Chinese medicine Radix isatidis, Background technique [0002] Banlangen (RADIXISATIDIS) is the dried root of Isatis indigotica Fort., a cruciferous plant. It has the effects of clearing heat and detoxifying, cooling blood and relieving throat. Throat dansha, big head plague, erysipelas, carbuncle [1] , Modern pharmacodynamic studies have shown that Banlangen has pharmacological effects such as antibacterial, antiviral, antiendotoxin, immune enhancement and antitumor [2-6] , at present, the quality control method of Radix isatidis is mainly based on the quantitative analysis of components such as indigo and indirubin [7，8] , not enough to control the quality of Banlangen as a whole, as a common clinical traditional Chinese medicine, its authenticity is affected by many factors such as its place of origin, harvest time, and dryi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/315A61P11/04A61P31/04A61P31/12A61P35/00A61P37/04G01N27/62G01N30/02G01N30/86A61K125/00
Inventor 刘志强闫峻刘舒皮子凤宋凤瑞刘忠英
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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