Method for one-step desalting and enriching on low-abundance protein target

A low-abundance protein enrichment technology, applied in the field of biochemical analysis, can solve the problems of limited protein or peptide adsorption, insufficient salt elution, loss, etc., and achieve the effects of reduced sample area, improved sensitivity, and high sensitivity
CN1811407AInactive Publication Date: 2006-08-02FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2006-08-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method capable of implementing on-target one-step desalination and enrichment of trace protein or polypeptide by means of combined application of hydrophobic polymer microblotting technique and matrix auxiliary laser analysis ionization mass spectrography. Said microblotting hydrophobic polymer has good adsorption and enrichment effect for protein and polypeptide, so that it can implement one-step desalination and direct MALDI-TOF-MS analysis. Said invention can be extensively used in the field of protein phylogeny.
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Description

Technical field

[0001] The invention belongs to the technical field of biochemical analysis, and specifically relates to a method for desalination and enrichment of trace samples. Background technique

[0002] With the smooth implementation of the Human Genome Project (HGP), people realized that functional genomics focuses on suggesting the biological functions of genes and clarifying the laws of gene activity at the overall level of the genome. Genes are only carriers of genetic information, and life activities The executor and embodiment of the gene is the expression product of the gene---protein. In the post-genomic era, the central task of life sciences is to clarify the expression rules and biological functions of all proteins that are expressed by the genome that actually perform life activities. That is, the research center of life sciences will shift from genomics to proteome. The proteome is much more complex than the genome. The development of proteomics requires not on...

Claims

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