5results about How to "Strong salt tolerance" patented technology

This invention relates to the field of broad bean paste technology and discloses a low-salt broad bean paste production process based on the synergistic fermentation of compound microorganisms. The process includes: uniformly mixing broad bean koji with drained chili embryos to obtain a solid mixture; spraying an A-phase initiation conditioning solution onto the solid mixture and inoculating it with *Lactobacillus plantarum*, mixing until moistened to obtain a primary fermentation mash; controlling the primary fermentation mash to undergo constant-temperature fermentation until the pH value drops to a preset acidic range to obtain an acidified mature mash; injecting a B-phase hypertonic regulating solution into the acidified mature mash and inoculating it with *Tetracoccus halophilus* and *Zygosacchariformis*, mixing uniformly to obtain a phase-inversion fermentation mash; cooling the phase-inversion fermentation mash and conducting anaerobic fermentation under air-isolated conditions to obtain the low-salt broad bean paste product. This invention solves the problems of insufficient umami and softening in low-salt fermentation by segmented salt control driving synergistic succession of microorganisms, combined with chemical regulation to protect enzyme activity and enhance texture, significantly improving the flavor and taste of the product.

The application discloses a kind of knock-out genes and its application in tomato salt stress tolerance, and the base sequence of the tomato gene is as shown in SEQ ID NO.1;The amino acid sequence of tomato SlERF.J2 protein encoded by SlERF.J2 gene is as shown in SEQ ID NO.2.The application obtains SlERF.J2 knock-out mutant strain by CRISPR / Cas9 technology, and it is found by combining salt stress experiment that the chlorophyll content of knockout plant is reduced at 300mM NaCl treatment, and the content of malondialdehyde (MDA) is reduced, indicating that the damage of lipid peroxidation is reduced.In addition, the expression of SlERF.J2 gene is inhibited under salt stress, suggesting that it may regulate tomato salt tolerance by regulating chloroplast function and antioxidant pathway.The application provides a theoretical basis for analyzing the molecular mechanism of ERF family genes and the breeding of tomato salt-tolerant varieties.

The invention discloses a composite microbial preparation and application thereof in treatment of mariculture wastewater, and relates to the technical field of microbial agents. According to the preparation method, bacillus velezensis, halobacillus dakamoyi and Starproteia are used as fermentation strains, fermentation is performed to obtain a fermentation bacterial liquid, and then gamma-polyglutamic acid, sodium alginate and calcium chloride are utilized to perform immobilization treatment on the fermentation bacterial liquid to prepare the compound microbial preparation. The prepared composite microbial preparation is added into the mariculture wastewater, so that the total nitrogen content, the total phosphorus content and the COD content in a water body can be effectively reduced, the efficient treatment of the mariculture wastewater is realized, and the water quality stability is improved. Wherein the bacillus velezensis, the halobacillus dakamoyi and the Starproteinia are combined for use, and a synergistic effect on reducing the removal efficiency of total nitrogen in the mariculture wastewater is achieved.

The application discloses a tackifier, a preparation method of the tackifier, a composite plug oil displacement agent and an enhanced oil recovery method. The tackifier contains structural unit A, structural unit B, structural unit C and structural unit D, and the mass ratio of the structural unit A, the structural unit B, the structural unit C and the structural unit D is (2000-20000):(2000-40000):(4000-60000):(6000-100000), n is 1-15, m is 1-20, and the viscosity average molecular weight of the tackifier is 45-50 million. The tackifier has the following advantages: under the condition of a temperature of 60-70 DEG C and a salinity of 10000 mg / L, the viscosity of a polymer solution is greater than 100 mPa s; when the concentration of the polymer solution is 1000 mg / L, the surface tension is less than 40 mN / m, the tackifier has the function of emulsifying and dispersing crude oil, and the oil displacement efficiency of a physical model is increased by more than 12%.

The invention discloses an all-weather self-adaptive light-electricity-water-hydrogen integrated floating device, and belongs to the technical field of seawater resource utilization and hydrogen energy preparation, the all-weather self-adaptive light-electricity-water-hydrogen integrated floating device comprises a base, the top of the base is provided with an electrolytic hydrogen production mechanism, the electrolytic hydrogen production mechanism comprises an electrolytic tank fixedly connected with the base, an electrolytic part is arranged in the electrolytic tank, the electrolytic tank is communicated with a hydrogen storage ring, and the hydrogen storage ring is communicated with a water storage tank. The top of the electrolytic cell communicates with a collecting mechanism, the collecting mechanism comprises a water collecting tank communicating with the electrolytic cell, the top of the water collecting tank communicates with a collecting part, the top of the collecting part is provided with an air cavity and a hydrogel interlayer, and a gap is formed between the air cavity and the hydrogel interlayer. Autonomous photovoltaic power supply, all-weather efficient water absorption and evaporation of hydrogel, rapid circulation of gas in the gas cavity and rapid condensation and liquefaction of the surface of the gas cavity can be realized, and liquefied fresh water is recovered and supplied to the electrolytic cell for electrolytic hydrogen production. According to the invention, 'seawater vapor collection 'and'photovoltaic electrolytic hydrogen production' can be completed at the same time, and the equipment cost and the occupied area are remarkably reduced.