21results about How to "Strong salt tolerance" patented technology

The invention relates to the technical field of biology, in particular to application of coding sites of soybean PRR5a and PRR5b genes in the aspect of regulating and controlling salt tolerance and application of a mutant material generated by the coding sites in the aspect of regulating and controlling the salt tolerance of soybeans. According to the invention, an effective target for editing soybean PRR5a and PRR5b genes is subjected to double-strand breakage under mediation of endonuclease Cas9, so that different allele mutants of PRR5a and PRR5b are obtained. By knocking out the PRR5a and PRR5b genes, a new strategy is provided for enhancing the salt tolerance of the soybeans, reliable means and materials are also provided for cultivating new varieties of the soybeans and improving the salt tolerance, and the method plays an important role in research and production of crops.

The invention discloses a method for strengthening formation and denitrification performance of salt-tolerant aerobic granular sludge in high-salinity wastewater treatment, which comprises the following specific implementation processes: culturing S-AGS by taking activated sludge of a town sewage treatment system and salt-tolerant sludge of an industrial wastewater treatment system as a mixed inoculum, and optimizing the operating conditions of an SBR (sequencing batch reactor); 16SrRNA high-throughput sequencing and co-occurrence network analysis are applied to identify S-AGS core species, a theoretical basis is provided for directional strengthening of structural stability and denitrification performance of the S-AGS core species, screening and expanding culture are performed on core functional strains, Thaura T-1 and PseudofuvimonasP-1 obtained through screening and expanding culture are compounded to prepare a composite functional microbial inoculum, and on the basis of mixed inoculum and reactor conditions, the composite functional microbial inoculum can be used for preparing the high-efficiency denitrification agent for the S-AGS. The composite functional microbial agent is added, so that the synergistic strengthening effect on the denitrification performance of the S-AGS is achieved. According to the method, mixed sludge is inoculated, efficient nitrogen and carbon removal performance is achieved under high salt stress, a core strain is screened and subjected to expanding culture and compounded to serve as a composite functional bacterial agent, and the nitrogen removal efficiency of nitrification / denitrification in the biological treatment process under salt stress can be improved.

The invention relates to a branched ultrahigh-salt and ultrahigh-temperature resistant polyacrylamide polymer as well as a preparation method and application thereof. The preparation method comprises the following steps: obtaining the polyacrylamide polymer with a branched structure by adopting a reducing agent octamethylpentaethylenehexamine; the branched polymer has a larger hydrodynamic volume and a denser intermolecular network structure, has better shear resistance, and can obtain a higher resistance factor and a higher residual resistance factor in a porous medium; and the problems of polymer molecule curl and obvious solution viscosity reduction caused by the electrostatic shielding effect can be weakened under the high-salt condition.

This invention relates to the field of broad bean paste technology and discloses a low-salt broad bean paste production process based on the synergistic fermentation of compound microorganisms. The process includes: uniformly mixing broad bean koji with drained chili embryos to obtain a solid mixture; spraying an A-phase initiation conditioning solution onto the solid mixture and inoculating it with *Lactobacillus plantarum*, mixing until moistened to obtain a primary fermentation mash; controlling the primary fermentation mash to undergo constant-temperature fermentation until the pH value drops to a preset acidic range to obtain an acidified mature mash; injecting a B-phase hypertonic regulating solution into the acidified mature mash and inoculating it with *Tetracoccus halophilus* and *Zygosacchariformis*, mixing uniformly to obtain a phase-inversion fermentation mash; cooling the phase-inversion fermentation mash and conducting anaerobic fermentation under air-isolated conditions to obtain the low-salt broad bean paste product. This invention solves the problems of insufficient umami and softening in low-salt fermentation by segmented salt control driving synergistic succession of microorganisms, combined with chemical regulation to protect enzyme activity and enhance texture, significantly improving the flavor and taste of the product.

The application discloses a kind of knock-out genes and its application in tomato salt stress tolerance, and the base sequence of the tomato gene is as shown in SEQ ID NO.1;The amino acid sequence of tomato SlERF.J2 protein encoded by SlERF.J2 gene is as shown in SEQ ID NO.2.The application obtains SlERF.J2 knock-out mutant strain by CRISPR / Cas9 technology, and it is found by combining salt stress experiment that the chlorophyll content of knockout plant is reduced at 300mM NaCl treatment, and the content of malondialdehyde (MDA) is reduced, indicating that the damage of lipid peroxidation is reduced.In addition, the expression of SlERF.J2 gene is inhibited under salt stress, suggesting that it may regulate tomato salt tolerance by regulating chloroplast function and antioxidant pathway.The application provides a theoretical basis for analyzing the molecular mechanism of ERF family genes and the breeding of tomato salt-tolerant varieties.

The invention relates to a bacillus sp. Strain for producing a lipopeptide surfactant and application thereof, the bacillus sp. Strain is named as R3-1, and is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and the preservation number is CGMCC No.30660. The invention further discloses a preparation method of the bacillus sp. Strain for producing the lipopeptide surfactant and application of the bacillus sp. Strain for producing the lipopeptide surfactant. The lipopeptide surfactant produced by culturing and fermenting the cyst bacillus strain R3-1 comprises a surfactin and a progen. Compared with the prior art, the bacillus sp. Strain disclosed by the invention can simultaneously generate two lipopeptide surfactants, is strong in acid and alkali resistance, salt resistance and temperature adaptability, has a relatively good emulsifying effect on various hydrocarbon substances, and also has relatively good emulsifying capacity at relatively low concentration. Therefore, the method has great application potential in the aspects of oil exploitation and environmental pollution treatment caused by oil sludge.

The invention provides a surfactant for drilling fluid as well as a preparation method and application of the surfactant, and belongs to the technical field of drilling compositions. The preparation method of the surfactant for the drilling fluid comprises the following steps: (1) carrying out ring-opening reaction on dihydric alcohol and maleic anhydride to obtain a first intermediate; (2) carrying out esterification reaction on the first intermediate and a long-chain alcohol compound under the action of a catalyst to obtain a second intermediate; and (3) carrying out sulfonation reaction on the second intermediate, sultone and alkali metal hydride to obtain the product. According to the invention, the surfactant is endowed with excellent salt resistance and high temperature resistance through two functional groups of anion (-OR4SO2M1) and nonionic (-COOR2), the biodegradability is good, and the environmental protection performance is excellent. In addition, the drilling fluid has good compatibility and good inhibition performance in drilling fluid, and is suitable for drilling operation of high-temperature and high-salinity carbonate reservoirs.

The present invention discloses a Bacillus subtilis N51, a bacterial agent, a preparation method and an application thereof, and belongs to the field of biotechnology. Bacillus subtilis N51 was deposited in the China General Microbiological Culture Collection Center on April 24, 2024, with the deposit address being No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.30428. The Bacillus subtilis N51 disclosed in the present invention has excellent salt tolerance. Under salt stress conditions, the strain can improve the stress resistance of plants, and can improve the plant height, root length, lateral root density, aboveground fresh weight and aboveground dry weight of plants. The present invention also discloses a method for preparing a bacterial agent. The method of the present invention can obtain a bacterial agent with a high number of mature spores, and the process is simple, which is conducive to the expansion of production and application of the strain.

The invention discloses a methanophilic strain for producing an extracellular polymeric substance and a method for producing the extracellular polymeric substance by using the methanophilic strain, and belongs to the technical field of industrial biology. The methanophilic strain for producing the extracellular polymeric substances is classified and named as extracellular polymeric substance methanophilic bacteria NG09, can grow by using methane or methanol as a unique carbon source, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 14, 2024, and has the preservation number of CGMCC No.30951. The methanophilic strain for producing the extracellular polymeric substances has the advantages that the methanophilic bacteria can be used for producing the extracellular polymeric substances, the methanophilic bacteria can be used for producing the extracellular polymeric substances, the methanophilic bacteria can be used for producing the extracellular polymeric substances, and the methanophilic bacteria can be used for producing the extracellular polymeric substances. When the methanophilic strain for producing the extracellular polymeric substance is used for producing the extracellular polymeric substance, carbon emission reduction is realized through microbial carbon sequestration, and a harmless preparation can be produced by utilizing a non-grain-based one-carbon raw material and is widely applied to the fields of environmental restoration, agricultural production and the like.

The invention discloses a method for preparing a high-salt phosphate-functionalized silicide material for efficient uranium extraction with ultra-low feed amount, comprising mixing phytic acid, ethanol and water in a certain proportion, adding vinyl triethoxysilane, stirring evenly, heating and reacting, naturally cooling to room temperature, centrifuging to obtain a precipitate, washing, and vacuum drying to obtain a phosphate-functionalized silicide material. The process is simple and easy, the synthesis conditions are mild, and the phosphate-functionalized silicide material prepared by the method of the invention has a fast uranium adsorption rate and excellent salt resistance at an ultra-low feed amount of 0.06 g / L.

This invention discloses genes nyxgjy1 This invention, applied to improving plant salt tolerance, falls under the field of molecular breeding technology. It creatively obtains a gene from moso bamboo, which is adapted to grow in slightly acidic soil, that enhances plant salt tolerance. nyxgjy1 Genes, genes nyxgjy1 The CDS sequence is shown in SEQ ID NO.3; the gene was expressed heterologously in rice. nyxgjy1 Overexpression was found nyxgjy1 Rice plants with this gene exhibit stronger salt tolerance, and the expression level is positively correlated with the plant's salt tolerance—the higher the expression level, the stronger the salt tolerance. nyxgjy1 Genes can be used for the selection of salt-tolerant plant varieties and the identification of salt tolerance, which is of great significance for shortening the breeding process, reducing the breeding workload, and accelerating the utilization / transformation of saline-alkali land.

The invention discloses a composite microbial preparation and application thereof in treatment of mariculture wastewater, and relates to the technical field of microbial agents. According to the preparation method, bacillus velezensis, halobacillus dakamoyi and Starproteia are used as fermentation strains, fermentation is performed to obtain a fermentation bacterial liquid, and then gamma-polyglutamic acid, sodium alginate and calcium chloride are utilized to perform immobilization treatment on the fermentation bacterial liquid to prepare the compound microbial preparation. The prepared composite microbial preparation is added into the mariculture wastewater, so that the total nitrogen content, the total phosphorus content and the COD content in a water body can be effectively reduced, the efficient treatment of the mariculture wastewater is realized, and the water quality stability is improved. Wherein the bacillus velezensis, the halobacillus dakamoyi and the Starproteinia are combined for use, and a synergistic effect on reducing the removal efficiency of total nitrogen in the mariculture wastewater is achieved.

The invention provides a supramolecular compound as well as a preparation method and application thereof. The supramolecular compound has a structure as shown in a formula I, the supramolecular compound provided by the invention is formed by the organic acid and the organic alkali through a counter-ion coupling effect and has extremely high surface activity, and the excellent surface performance endows the supramolecular compound with outstanding foamability and foam stability, so that the stability of a liquid-gas interface is facilitated; in addition, the supramolecular compound provided by the invention also has good salt tolerance, is wide in use temperature range, and can meet the application requirements of foam flooding in high-temperature and high-salt oil fields.

The application discloses a tackifier, a preparation method of the tackifier, a composite plug oil displacement agent and an enhanced oil recovery method. The tackifier contains structural unit A, structural unit B, structural unit C and structural unit D, and the mass ratio of the structural unit A, the structural unit B, the structural unit C and the structural unit D is (2000-20000):(2000-40000):(4000-60000):(6000-100000), n is 1-15, m is 1-20, and the viscosity average molecular weight of the tackifier is 45-50 million. The tackifier has the following advantages: under the condition of a temperature of 60-70 DEG C and a salinity of 10000 mg / L, the viscosity of a polymer solution is greater than 100 mPa s; when the concentration of the polymer solution is 1000 mg / L, the surface tension is less than 40 mN / m, the tackifier has the function of emulsifying and dispersing crude oil, and the oil displacement efficiency of a physical model is increased by more than 12%.

The present invention relates to the field of genetic engineering technology, and in particular to an insertion-deletion molecular marker of a rice salt-tolerant gene OsCam1-1, and its application, primer pair and identification method. The physical position of the insertion-deletion molecular marker is located at the 1205bp to 1215bp of the promoter genome sequence of the rice salt-tolerant gene OsCam1-1, and the base is ACATGATTGA, and the salt-tolerant rice variety lacks the base. By screening molecular markers closely linked to the salt-tolerant gene, rice varieties with strong salt tolerance can be identified. The molecular markers of the present invention can be used for the identification and selection of salt-tolerant genotypes during the germination period of rice seeds, and individuals carrying excellent salt-tolerant alleles can be obtained, which can overcome the shortcomings of conventional breeding methods such as the long time period required, and cultivate new rice varieties with strong salt tolerance.

The invention provides a saline-alkali land drainage ditch plant slope fixing method, which belongs to the technical field of plant slope fixing, and comprises the following steps: S1, constructing ridges and ditches: in spring or autumn, digging ridges and ditches on the slope surface of the drainage ditch, 45-55 cm above the highest water level, and building a ridge every 30-40 m; S2, irrigating and pressurizing salt water: before planting, irrigating and pressurizing salt water into the ridges and ditches; S3, selecting plant varieties: selecting tamarisk and weeping willow as planting seedlings; S4, digging planting holes: when the ridges and ditches are dry and the soil is not too sticky to the shovel, digging planting holes; S5, transplanting: transplanting weeping willows and tamarisks alternately and at intervals into the planting holes dug in step S4; the time interval from seedling removal to planting of tamarisks and weeping willows is ≤3 days; S6, irrigating seedling protection water; irrigating seedling protection water in time after transplanting, and irrigating thoroughly; S7, management and maintenance; the invention adopts the method of planting weeping willows and tamarisks on the slope of the drainage ditch, and using the plant root system to protect the slope of the drainage ditch, and the slope protection effect is remarkable.

The present invention discloses a rose RcMYB14 transcription factor and its application; the nucleotide sequence of the rose 'Yueyuefen' RcMYB14 transcription factor is shown in SEQ ID No.1; the sequence of the corresponding protein is shown in SEQ ID NO:2. The RcMYB14 gene can be induced to express by salt stress and high temperature stress. The gene is transformed into tobacco and Arabidopsis thaliana by Agrobacterium-mediated genetic transformation, which can significantly reduce the damage of plants caused by salt and high temperature stress, and can increase the growth of transgenic Arabidopsis thaliana, and significantly enhance the salt tolerance and heat tolerance of transgenic plants. The present invention lays a foundation for the study of the molecular mechanism of plant salt tolerance and high temperature resistance, and provides excellent genes for the future breeding of salt-tolerant and high temperature-resistant plant varieties by molecular technology; in addition, the transcription factor of the present invention provides an important possibility for breeding stress-resistant rose varieties, which has practical significance for the production of roses.

The invention discloses an all-weather self-adaptive light-electricity-water-hydrogen integrated floating device, and belongs to the technical field of seawater resource utilization and hydrogen energy preparation, the all-weather self-adaptive light-electricity-water-hydrogen integrated floating device comprises a base, the top of the base is provided with an electrolytic hydrogen production mechanism, the electrolytic hydrogen production mechanism comprises an electrolytic tank fixedly connected with the base, an electrolytic part is arranged in the electrolytic tank, the electrolytic tank is communicated with a hydrogen storage ring, and the hydrogen storage ring is communicated with a water storage tank. The top of the electrolytic cell communicates with a collecting mechanism, the collecting mechanism comprises a water collecting tank communicating with the electrolytic cell, the top of the water collecting tank communicates with a collecting part, the top of the collecting part is provided with an air cavity and a hydrogel interlayer, and a gap is formed between the air cavity and the hydrogel interlayer. Autonomous photovoltaic power supply, all-weather efficient water absorption and evaporation of hydrogel, rapid circulation of gas in the gas cavity and rapid condensation and liquefaction of the surface of the gas cavity can be realized, and liquefied fresh water is recovered and supplied to the electrolytic cell for electrolytic hydrogen production. According to the invention, 'seawater vapor collection 'and'photovoltaic electrolytic hydrogen production' can be completed at the same time, and the equipment cost and the occupied area are remarkably reduced.

The invention discloses application of a peanut AhNF-YA3 gene in improving the oil content and / or salt tolerance of plants, and belongs to the technical field of gene engineering. The nucleotide sequence of the peanut AhNF-YA3 gene for improving the oil content and / or the salt tolerance of the plant is as shown in SEQ ID NO: 1. After the gene is used for constructing a plant expression vector and transforming peanuts, the oil content of peanut seed kernels can be remarkably improved, and the oil content is at least improved by 3.9 percent compared with that of wild types; the salt tolerance can be obviously improved, at least 350mM NaCl can be tolerated, and the method has a relatively good application prospect in the field of improving the oil content and / or the salt tolerance of the plants.

The invention discloses bacillus subtilis N51 as well as a fungicide, a preparation method and application thereof, and belongs to the technical field of biology. The bacillus subtilis N51 is preserved in the China General Microbiological Culture Collection Center on April 24, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.30428. The bacillus subtilis N51 is preserved in the China General Microbiological Culture Collection Center. The bacillus subtilis N51 disclosed by the invention has excellent salt tolerance, and under the condition of salt stress, the strain can improve the stress resistance of plants, and can improve the plant height, the root length, the lateral root density, the overground fresh weight and the overground dry weight of the plants. The invention further discloses a preparation method of the microbial inoculum, the microbial inoculum with high mature spore number can be obtained through the method, the process is simple and convenient, and expanded production and application of strains are facilitated.

The invention relates to the technical field of oilfield chemistry, in particular to a salt-assisted drilling and grinding fluid system as well as a preparation method and application thereof, the salt-assisted drilling and grinding fluid system comprises the following raw materials in percentage by mass: 1.8-3.2% of a salt inhibitor, 1.5-2% of a tackifier, 0.5-2% of a lubricant and the balance of water; the salt inhibitor is one or more of metal complexing agents, and the lubricant is modified silicone grease. According to the salt hydrotropy drilling and grinding fluid system, solubilization and crystallization inhibition effects on salt scale of a high-salt-content oil and gas reservoir can be achieved, and the phenomena that a production channel is blocked and a pipe column is blocked by salt due to underground salt crystallization of the high-salt-content oil and gas reservoir can be effectively avoided; and during mechanical drilling and grinding dredging operation, drilling and grinding liquid is dissolved and scales are formed, the drilling and grinding time efficiency is improved, and the risks of damage, wellhead stabbing leakage and the like caused by sudden release of high pressure to eject an oil pipe are reduced. In addition, the hydrotropy drilling and grinding fluid system further has good temperature resistance and salt resistance, good resistance reduction performance and good chip carrying capacity.